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Vetiveria zizanioides , renowned for its robust stability and exceptional capacity to sequester heavy metals, has garnered widespread application in tailings ecological restoration efforts. Arbuscular mycorrhizal fungi (AMF), which are capable of forming symbiotic relationships with more than 80% of terrestrial plant roots, play a pivotal role in enhancing plant nutrient uptake and bolstering resilience. In this study, we conducted a comprehensive investigation into the physiological and biochemical responses of Vetiveria zizanioides subjected to varying levels of copper stress (with copper concentrations ranging from 0 mg/kg to 400 mg/kg), with or without AMF inoculation. Additionally, we performed nontargeted metabonomic analyses to gain deeper insights into the metabolic changes that occur in vetiver grass under AMF inoculation and copper stress. Our findings revealed that Vetiveria zizanioides inoculated with AMF consistently demonstrated superior growth performance across all copper stress levels compared with noninoculated counterparts. Using nontargeted metabonomic analyses, inoculation with AMF affects the metabolism of phenylalanine and related pathways in vetiver as well as contributing to the promotion of the formation of phytochelatins (PCs) from glutamate, thereby alleviating copper stress. The results highlight the potential of AMF-inoculated Vetiveria zizanioides as a promising bioremediation tool capable of effectively mitigating the adverse effects of heavy metal pollution.

Salinity represents a major environmental factor limiting plant growth and productivity. In order to better understand the effects of arbuscular mycorrhizal fungus Claroideoglomus etunicatum and Indole-3-acetic acid (IAA) on the growth and chemical composition of vetiver grass (Vetiveria zizanioides) under salt stress, a factorial experiment was conducted in a completely randomized design with three replications. The experiment included four NaCl levels (0, 8, 16, and 24 decisiemens per meter (dS/m)) and four levels of treatments (no amendment application, application of IAA, application of C. etunicatum, and interaction of IAA and C. etunicatum) with three replications. The results of the experiment showed that the addition of sodium chloride increased the concentration of proline and the activities of catalase, peroxidase, and superoxide dismutase enzymes. The application of the growth regulator (IAA) and C. etunicatum significantly increased the fresh and dry weight (101%) of shoots, dry weight of roots, and the concentration of macro- and micro-elements in shoots under salinity condition (99.82% phosphorus; 9.79% Iron). The application of mycorrhiza and auxin significantly reduced the concentration of proline and the activities of catalase, peroxidase, and superoxide dismutase enzymes. In general, the addition of IAA and C. etunicatum to roots under salt stress conditions can improve growth and increase the concentration of some nutrients in vetiver shoots.

Phytoremediation of contaminated mine soils requires the use of fast-growing, deep-rooted, high-biomass, and metal-tolerant plants with the application of soil amendments that promote metal uptake by plants. A pot experiment was performed to evaluate the combined use of vetiver grass ( Chrysopogon zizanioides ) and humic acid for phytoremediation of Cu and Zn in mine soils. Vetiver plants were grown in soil samples collected from two mine sites of Spain mixed with a commercial humic acid derived from leonardite at doses of 0, 2, 10, and 20 g kg −1 . Plant metal concentrations and biomass were measured and metal bioavailability in soils was determined by a low molecular weight organic acid extraction. Results showed that humic acid addition decreased organic acid-extractable metals in soil. Although this extraction method is used to estimate bioavailability of metals, it was not a good estimator under these conditions due to competition with the strong chelators in the added humic acid. High doses of humic acid also promoted root growth and increased Cu concentrations in plants due to formation of soluble metal-organic complexes, which enhanced removal of this metal from soil and its accumulation in roots. Although humic acid was not able to improve Zn uptake, it managed to reduce translocation of Zn and Cu to aerial parts of plants. Vetiver resulted unsuitable for phytoextraction, but our study showed that the combined use of this species with humic acid at 10–20 g kg −1 could be an effective strategy for phytostabilization of mine soils.

India’s fast industrialization and population expansion have resulted in heavy metal accumulation from many operations, which has caused massive waste generation and poisoning of soils. Therefore, it is necessary to design reclamation to improve th T.Ne soil. Phytoremediation presents itself as a viable, economical, and environmentally sustainable solution to this problem. This study was carried out by using plants namely, aloe-vera (Aloe-Barbadensis), tulsi (Ocimum Tenuiflorium), and vetiver (Chrysopogon Zizanoides) plants which were planted in a simulated soil of Cd, Zn and Pb, for 4 weeks. The sample of plant and soil were taken in 9 different pots, (15 cm diameter and 25 cm height) among 9 potted soils one will be tested as a controlled sample. An aqueous solution of lead, cadmium and zinc were added separately to the dry soil samples. The moisture level of the soil was maintained to near field water capacity (35.6%) and equilibrated for two weeks. The saplings of vetiver grass, aloe vera and tulsi were selected and pruned (the shoots were originally 20 cm high and the roots 8 cm long), and then transplanted into the pots. The AAS test was conducted after 4 weeks of growing in simulated soil. Tulsi demonstrated the highest efficacy in reducing Zn concentrations from 300 mg/kg to 188.3 mg/kg, followed by vetiver (179.3 mg/kg) and Aloe vera (158.3 mg/kg). Similarly, for Pb, tulsi exhibited the most substantial reduction (from 600 mg/kg to 188.3 mg/kg), followed by vetiver (164.3 mg/kg) and Aloe vera (179.6 mg/kg). Regarding Cd, tulsi reduced concentrations from 80 mg/kg to 18.62 mg/kg, while vetiver achieved a 17.62 mg/kg reduction. The result highlights Tulsi’s superior remediation potential, attributed to its efficient heavy metal uptake and translocation mechanisms. Thus, using these plants in the phytoremediation process, the heavy metals are extracted more economically than other plants. This technique highlights the innate ability of hyper-accumulator plant species, which flourish in situations high in heavy metals, to extract contaminants from contaminated soil.

Generally, there are scant data about the constituents and eventually the biological activity of essential oils (EOs) from aromatic plants that grow naturally in Sudan. The present study aimed to determine the chemical composition, and antioxidant and enzyme inhibitory activities of EO extracted from the fruit of Chamaecyparis obtusa (Siebold and Zucc.) Endl. (family Cupressaceae), root of Chrysopogon nigritanus (Benth.) Veldkampis (family Poaceae) and aerial part of Lavandula coronopifolia Poir (family Lamiaceae). The fruit of C. obtusa contained only monoterpenes, mainly hydrogenated ones, with α-pinene (69.07%) as the major component. Oxygenated sesquiterpenes comprised the highest content of the C. nigritanus root EO with cedr-8-en-15-ol (28.69%) as the major constituent while aerial parts of L. coronopifolia contained both monoterpenes and sesquiterpenes and the oxygenated monoterpene lavandulol (26.56%) as dominant compounds. The EO of the root of C. nigritanus significantly displayed (p < 0.05) the highest anti-DPPH radical, Fe3+- and Cu2+-reducing and metal-chelating activities, while that of C. obtusa fruit significantly exerted (p < 0.05) the best anti-ABTS radical and total antioxidant activity. The two EOs significantly exhibited (p < 0.05) the highest anti-acetylcholinesterase and -butyrylcholinesterase activities, respectively, while EO of L. coronopifolia was the only oil to show a considerable inhibitory effect against the tyrosinase and α-glucosidase enzymes. In conclusion, EOs from these three plants could be natural agents with promising functional properties for food, cosmetics, and pharmaceutical applications.

Grassroots have received more attention than the traditional method as soil reinforcement materials, especially the use of vetiver and other vegetation protection methods to treat expansive soil slope, have been tried and applied. To study the influence of grassroots on the strength properties of expansive soil, the laws of vetiver root growth over time and its vertical distribution of root content( δ ) were firstly investigated by the experiment of planting vetiver. Then different δ and depth of planted soil were obtained. Simultaneously different δ and water content( ω ) of grafted soil were made. With the direct shear test, the shear strength parameters of root-soil with different δ were analyzed. The shear test on root-soil composites with different δ was carried out to compare the strength characteristics of planted and grafted soil. The results showed that the δ of vetiver decreased with the increase of depth, and the δ of each layer increased with the growth period. The δ of 180d was 70.5% higher than that of 90d. The cohesion( c ) of root-soil can be increased by more than 97%, and internal friction angle( φ ) can be increased by more than 15.4% after 180 days. The c of 90 d vetiver root system can be increased by more than 18%, and the φ can be increased by more than 1.5%. At each depth, the c and φ of composite soil increases with the increase of δ , and the increment of cohesion (Δ c ) and the increment of internal friction angle (Δ φ ) increase with the increment of δ . But the increase in the ω will weaken the shear strength parameters of root-soil. Under the condition of the planted root system and grafted root system, the influence degree of δ on strength parameter of root-soil is different, and the law of strength parameters versus δ of grafted soil of 365d is similar to that of planted soil of 90d. And the root reinforcement of grafted soil is weaker than planted soil. Hence the grafted soil can´t accurately reflect the root-soil interaction of the existing root system.

Soil contamination by cadmium (Cd) poses a serious environmental and public health concern. Phytoremediation, i.e., the use of plants to remove contaminants from soil, has been proposed for treatment of Cd-contaminated ecosystems. In this study, we demonstrated the potential of Vetiveria zizanioides , commonly known as vetiver, to serve as an effective phytoremediation agent. Two ecotypes, i.e., India and Sri Lanka, were grown in greenhouse pots and in the field. Soils were amended with cow manure, pig manure, bat manure, and an organic fertilizer. Among all amendments, pig manure performed best in both greenhouse and field studies in terms of increasing total V. zizanioides biomass production in both ecotypes. In both greenhouse and in the field, tissue of the Sri Lanka ecotype had higher Cd concentrations than did the India ecotype. In the greenhouse, the presence of Cd did not affect total biomass production or root dry weight. The Sri Lanka ecotype had 2.7 times greater adventitious root numbers and 3.6 times greater Cd accumulation in roots than did the India ecotype. In the field study, the Sri Lanka ecotype offers potential as an excluder species, as it accumulated Cd primarily in roots, with translocation factor values <1 and a bioconcentration coefficient for roots >1 for all experiments except for the pig manure amendment. In addition, the highest Cd concentration in the Sri Lanka ecotype root (71.3 mg kg −1 ) was consistent with highest Cd uptake (10.4 mg plant −1 ) in the cow manure treatment. The India ecotype contained lower root Cd concentrations, and Cd accumulation was slightly higher in shoots compared to roots, with translocation factor (TF) values >1. The India ecotype was therefore not considered as an excluder in the Cd-contaminated soil. With the use of excluder species combined with application of organic amendments, soil contamination by Cd may be treated by alternative remediation methods such as phytostabilization.

Environmental contamination by antibiotics not only perturbs the ecological balance but also poses a risk to human health by promoting the development of multiantibiotic-resistant bacteria. This study focuses on identifying the biochemical pathways associated with tetracycline (TC) transformation/degradation in vetiver grass that has the potential to be used as a biological remediation system in TC-contaminated water sources. A hydroponic experimental setup was used with four initial TC concentrations (0, 5, 35, 75 ppm), and TC uptake was monitored over a 30-day period. Results show that TC transformation in the media occurred during the first 5 days, where a decrease in the parent compound and an increase in the concentration of the isomers such as epitetracycline (ETC) and anhyrotetracycline (ATC) occurred, and TC disappeared in 20 days in tanks with vetiver grass. However, the isomers ETC and ATC remained in the control tanks for the duration of the trial. Transformation products of TC in plant tissue were analyzed by using ultra HPLC high-resolution Orbitrap mass spectrometery (HRMS/MS), which indicates amide hydrolysis of TC in vetiver roots. Metabolic profiling revealed that glyoxylate metabolism, TCA cycle, biosynthesis of secondary metabolites, tryptophan metabolism, and inositol phosphate metabolism were impacted in vetiver root by TC treatment.

Vetiver grass (Vetiveria zizanioides L. Nash) has a great application potential to the phytoremediation of heavy metals pollution. However, few studies explored the bioavailability and distribution of different speciations of As and Sb in V. zizanioides. This study aimed to clarify the allocation and accumulation of two inorganic species arsenic (As(III) and As(V)) and antimony (Sb(III) and Sb(V)) in V. zizanioides, to understand the self-defense mechanisms of V. zizanioides to these metal(loids) elements. Thus, an experiment was conducted under greenhouse conditions to identify distribution of As and Sb in plant roots and shoots. Antioxidant enzymes (superoxide dismutase, SOD) and changes of subcellular structures were tested to evaluate metal(loids) tolerance capacities of V. zizanioides. This study demonstrated that V. zizanioides had higher capacity to accumulate Sb than As. For Sb absorption, Sb(III) content is significantly higher than Sb(V) in tissues of V. zizanioides under all concentration levels, despite the oxidation of Sb(III) on the nutrient solution surface. Additional Sb was mainly accumulated in plant roots due to Sb immobilization by transforming it into precipitates. As was more easily transferred to aerial tissues and had low accumulation rates, probably due to its restricted uptake rather than restricted transport. In many cases, two inorganic species of As and Sb showed almost same biotoxicity to V. zizanioides estimated from its biomass, SOD activity, and MDA content as well as functional groups. In summary, the results of this study provide new insights into understanding allocation, accumulation and phytotoxicity effects of arsenic and antimony in V. zizanioides.Graphic abstractSchematic diagram of distribution of and biochemical responses to As(III), As(V), Sb(III), and Sb(V) in tissue of V. zizanioides.

Wastewater pollution is a pressing global environmental issue, which is intensified by urbanization and agricultural expansion. Rapid urbanization has resulted in increased municipal wastewater generation, necessitating the development of sustainable and effective treatment methods. The current study examines vetiver grass ( Vetiveria zizanioides ) as a phytoremediation method for municipal wastewater treatment, focusing on its ability to remove biochemical oxygen demand (BOD), chemical oxygen demand (COD), nitrogen (N), and phosphorus (P). Three vetiver plant densities (20, 40, and 60 plants/m 2 ) were evaluated to determine the optimal configuration for pollutant removal over a 9-week treatment period. The results demonstrated that a high-planting density vetiver (60 plants/m 2 ) achieved the highest overall removal efficiencies, reaching 89.7, 80.6, 60.5, and 40.8% for BOD, COD, nitrogen, and phosphate, respectively. These findings highlight the potential of high-planting density vetiver grass as a cost-effective and environmentally sustainable alternative for municipal wastewater treatment, providing valuable insights into planting density optimization under local Bahir Dar conditions and enabling scalable phytoremediation in resource-limited urban areas.