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The aim of this study was to investigate the antibacterial interactions of pulegone and 1,8‐cineole with monolaurin ornisin against Staphylococcus aureus. The individual and combined antibacterial activities of the compounds were evaluated using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration index (FICi), and time‐kill methods. Furthermore, the mechanism of the antibacterial action of the compounds was tested by measuring the release of cell constituents. The MIC values of pulegone, 1,8‐cineole, nisin, and monolaurin were 5.85 µl/ml, 23.43 µl/ml, 6.25 µg/ml, and 0.031 mg/ml, respectively. A synergistic antibacterial activity (FICi = 0.5) was found between 1,8‐cineole and nisin. The time‐kill assay showed that the populations of S. aureus exposed to 1,8‐cineole, nisin, and their combination were decreased by 5.9, 5.3, and 7.1 log CFU (colony‐forming units)/mL, respectively. The combination of 1,8‐cineole and nisin also induced the highest release of cell constituents. It was concluded that the combination of 1,8‐cineole and nisin could be considered as a novel and promising combination which may reduce the required dose of each antibacterial compound. The antibacterial activity of pulegone and 1,8‐cineole combined with monolaurin or nisin was investigated against Staphylococcus aureus. A synergistic antibacterial effect was found between 1,8‐cineole and nisin. This combination also induced the highest release of cell constituents. Therefore, the combination of 1,8‐cineole and nisin could be considered as a novel and effective antibacterial combination to control S. aureus.

Terpenoid-based essential oils are economically important commodities, yet beyond their biosynthetic pathways, little is known about the genetic architecture of terpene oil yield from plants. Transport, storage, evaporative loss, transcriptional regulation and precursor competition may be important contributors to this complex trait. Here, we associate 2.39 million single nucleotide polymorphisms derived from shallow whole-genome sequencing of 468 Eucalyptus polybractea individuals with 12 traits related to the overall terpene yield, eight direct measures of terpene concentration and four biomass-related traits. Our results show that in addition to terpene biosynthesis, development of secretory cavities, where terpenes are both synthesized and stored, and transport of terpenes were important components of terpene yield. For sesquiterpene concentrations, the availability of precursors in the cytosol was important. Candidate terpene synthase genes for the production of 1,8-cineole and α-pinene, and β-pinene (which comprised > 80% of the total terpenes) were functionally characterized as a 1,8-cineole synthase and a β/α-pinene synthase. Our results provide novel insights into the genomic architecture of terpene yield and we provide candidate genes for breeding or engineering of crops for biofuels or the production of industrially valuable terpenes.

Altering the radiation intensity in controlled environments can influence volatile organic compound (VOC) biosynthetic pathways, including those of terpenoids and phenylpropanoids. In turn, the concentrations of these compounds can have a profound effect on flavor and sensory attributes. Because sweet basil ( Ocimum basilicum ) is a popular culinary herb, our objectives were to (1) determine the extent radiation intensity and carbon dioxide (CO 2 ) concentration influence seedling terpenoid and phenylpropanoid concentrations; (2) determine if differences in phenylpropanoid and terpenoid concentrations influence consumer preference; and (3) characterize consumer preferences to better inform production and marketing strategies. “Nufar” sweet basil was grown with CO 2 concentrations of 500 or 1,000 μmol ⋅ mol –1 under sole-source radiation intensities of 100, 200, 400, or 600 μmol ⋅ m –2 ⋅ s –1 with a 16 h photoperiod to create daily light integrals of 6, 12, 23, and 35 mol ⋅ m –2 ⋅ d –1 . After 2 weeks, concentrations of the terpenoids 1,8 cineole and linalool and the phenylpropanoids eugenol and methyl chavicol were quantified, and consumer sensory panel evaluations were conducted to quantify preferences. Overall, increasing radiation intensity from 100 to 600 μmol ⋅ m –2 ⋅ s –1 increased 1,8 cineole, linalool, and eugenol concentrations 2. 4-, 8. 8-, and 3.3-fold, respectively, whereas CO 2 concentration did not influence VOCs. Contrary to our hypothesis, increased VOC concentrations were not correlated with consumer preference. However, overall liking was correlated with aftertaste and flavor. The conclusion that consumer preference is dependent on flavor can be drawn. However, increasing VOC concentrations to increase flavor did not improve flavor preference. Many consumer sensory preference characteristics (favorable preference for aftertaste, bitterness/sweetness, color, flavor, overall liking, and texture) were correlated with basil grown under a radiation intensity of 200 μmol ⋅ m –2 ⋅ s –1 . This led us to determine that consumers prefer to detect the characteristic basil flavor made up of 1,8 cineole, eugenol, and linalool, which was not as prevalent in basil grown under 100 μmol ⋅ m –2 ⋅ s –1 , but too high in basil grown under 400 and 600 μmol ⋅ m –2 ⋅ s –1 , which led to lower consumer preference.

Rosmarinus officinalis L. is an imperative herb used in pharmaceutical yet knowledge on chemical and activity profile of essential oil (EO) to harvest seasons and accessions from the Himalayan region is limited. Thus, accessions were evaluated to determine the EO content, compositional, antimicrobial, and cytotoxic potential of rosemary in different harvest seasons during 2018‒2019. EO content was 30.5% higher in IHBT/RMAc-1 compared with IHBT/RMAc-2 accession while 27.9% and 41.6% higher in the autumn as compared with summer and rainy season, respectively. Major EO compound was 1,8-cineole; ranged from 32.50‒51.79% during harvest seasons and 38.70‒42.20% in accessions. EO was active against both the tested Gram-positive bacteria ( Micrococcus   luteus MTCC 2470 and Staphylococcus aureus MTCC 96). EOs showed inhibition of Gram-negative bacteria ( Salmonella typhi MTCC 733), while Klebsiella pneumoniae MTCC 109 was found to be resistant. The rosemary EO of T1 (Rainy season IHBT/RMAc-1) was most effective against S. aureus MTCC 96 with the minimum inhibitory concentration (MIC) of 4% (v/v). In vitro cytotoxicity evaluation showed no potential anti-proliferative activity of EO. The rosemary EO profile in the western Himalayan region was influenced by harvesting seasons and genetic variability within the accessions; furthermore, a promising antibacterial agent in pharmaceutical and flavour industries.

is a genus from the family Lamiaceae, whose essential oils has long been used in various forms such as in management of plant pathogens and insect pests, in traditional medicine as well as in culinary and cosmetics. Its major chemical components such as menthol, carvone have now been successfully commercialized in the industry as antimicrobials/insecticidal agents. Current review focuses on chemical composition of essential oils of some species from different geographical regions with their insecticidal (repellent, antifeedant, and ovicidal) and antimicrobial efficacies against bacterial, fungal plant pathogens and insects of stored products. Reports of the researchers on chemical analysis of essential oils of species revealed that most of the oils being rich in pulegone, menthon, menthol, carvone, 1, 8-cineole, limonene and β-caryophyllene. Reviewed literature revealed that, essential oils from different species possess potential antimicrobial activity against plant pathogens and have insecticidal activity against stored product insects. Thus, antimicrobial and insecticidal properties of essential oils of species offer the prospect of using them as natural pesticides with a commercial value, having social acceptance due to its sustainability and being environment friendly.

The essential oil from Rosmarinus officinalis L., a composite mixture of plant-derived secondary metabolites, exhibits antifungal activity against virulent candidal species. Here we report the impact of rosemary oil and two of its components, the monoterpene α-pinene and the monoterpenoid 1,8-cineole, against Candida albicans , which induce ROS-dependent cell death at high concentrations and inhibit hyphal morphogenesis and biofilm formation at lower concentrations. The minimum inhibitory concentrations (100% inhibition) for both rosemary oil and 1,8-cineole were 4500 μg/ml and 3125 μg/ml for α-pinene, with the two components exhibiting partial synergy (FICI = 0.55 ± 0.07). At MIC and 1/2 MIC, rosemary oil and its components induced a generalized cell wall stress response, causing damage to cellular and organelle membranes, along with elevated chitin production and increased cell surface adhesion and elasticity, leading to complete vacuolar segregation, mitochondrial depolarization, elevated reactive oxygen species, microtubule dysfunction, and cell cycle arrest mainly at the G1/S phase, consequently triggering cell death. Interestingly, the same oils at lower fractional MIC (1/8-1/4) inhibited virulence traits, including reduction of mycelium (up to 2-fold) and biofilm (up to 4-fold) formation, through a ROS-independent mechanism.

Synergistic interactions between constituents of essential oils have been reported for several areas of research. In the present study, mechanisms that could explain the synergistic action of the two major insecticidal constituents of rosemary oil, 1,8-cineole and camphor against the cabbage looper, Trichoplusia ni were investigated. 1,8-Cineole was more toxic than camphor when applied topically to larvae and when coadministered in their ratio naturally occurring in rosemary oil, the binary mixture was synergistic. However, when injected directly into larvae, camphor was more toxic than 1,8-cineole. GC-MS analyses showed that penetration of topically-applied camphor was significantly enhanced when it was mixed with 1,8-cineole in the natural ratio. A bioassay combining injection and topical application methods confirmed the increased penetration of both compounds when mixed, showing the same bioactivity as seen for higher amounts applied individually. Lowered surface tension as well as increased solubility of camphor by 1,8-cineole, along with the interaction between 1,8-cineole and the lipid layer of the insect’s cuticle may explain the enhanced penetration of camphor. Because of the similarities in biological function of animal and microbial membranes, our finding has potential for application in other fields of study.

Medicinal or herbal spices are grown in tropical moist evergreen forestland, surrounding most of the tropical and subtropical regions of Eastern Himalayas in India (Sikkim, Darjeeling regions), Bhutan, Nepal, Pakistan, Iran, Afghanistan, a few Central Asian countries, Middle East, USA, Europe, South East Asia, Japan, Malaysia, and Indonesia. According to the cultivation region surrounded, economic value, and vogue, these spices can be classified into major, minor, and colored tropical spices. In total, 24 tropical spices and herbs (cardamom, black jeera, fennel, poppy, coriander, fenugreek, bay leaves, clove, chili, cassia bark, black pepper, nutmeg, black mustard, turmeric, saffron, star anise, onion, dill, asafoetida, celery, allspice, kokum, greater galangal, and sweet flag) are described in this review. These spices show many pharmacological activities like anti-inflammatory, antimicrobial, anti-diabetic, anti-obesity, cardiovascular, gastrointestinal, central nervous system, and antioxidant activities. Numerous bioactive compounds are present in these selected spices, such as 1,8-cineole, monoterpene hydrocarbons, γ-terpinene, cuminaldehyde, trans-anethole, fenchone, estragole, benzylisoquinoline alkaloids, eugenol, cinnamaldehyde, piperine, linalool, malabaricone C, safrole, myristicin, elemicin, sinigrin, curcumin, bidemethoxycurcumin, dimethoxycurcumin, crocin, picrocrocin, quercetin, quercetin 4’-O-β-glucoside, apiol, carvone, limonene, α-phellandrene, galactomannan, rosmarinic acid, limonene, capsaicinoids, eugenol, garcinol, and α-asarone. Other than that, various spices are used to synthesize different types of metal-based and polymer-based nanoparticles like zinc oxide, gold, silver, selenium, silica, and chitosan nanoparticles which provide beneficial health effects such as antioxidant, anti-carcinogenic, anti-diabetic, enzyme retardation effect, and antimicrobial activity. The nanoparticles can also be used in environmental pollution management like dye decolorization and in chemical industries to enhance the rate of reaction by the use of catalytic activity of the nanoparticles. The nutritional value, phytochemical properties, health advantages, and both traditional and modern applications of these spices, along with their functions in food fortification, have been thoroughly discussed in this review

The terpenoids are a major class of natural products with a range of functions in plants. In the grape, monoterpenes, sesquiterpenes and C₁₃‐norisoprenoids are present either in a free volatile form, or bound to sugars and rendered non‐volatile. Of the many terpenoids in grapes, only a relatively small number makes an important contribution to wine aroma. This review summarises new developments in understanding terpenoids, including the monoterpenes linalool, cis‐rose oxide, 1,8‐cineole and wine lactone; the sesquiterpene rotundone; and the C₁₃‐norisoprenoids β‐damascenone, β‐ionone, 1,1,6‐trimethyl‐1,2‐dihydronaphthalene and (E)‐1‐(2,3,6‐trimethylphenyl)buta‐1,3‐diene. Their origin, formation and behaviour during winemaking and ageing, and viticultural influences on their concentration, will be discussed, as well as their role in different wine styles.

According to the main volatile components of leaf essential oils (LEOs), such as monoterpenes, sesquiterpenes and diterpenes, camphor trees can be subdivided into five chemotypes: the borneol, camphor, cineole, linalool and nerolidol types. Through ab initio modelling, protein-based searches and transcript analysis of long-read isoform sequencing and short-read RNA sequencing data, a high-confidence set of 29 919 protein-coding gene models (concealing 37 295 protein-coding transcripts) was predicted by Maker2 in the C. camphora genome and was located on the 12 pseudochromosomes. Acknowledgements This work is supported by grants from the National Natural Science Foundation of China (Grant Nos. 32060354, 31860079 and 32160397) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).