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4,425 result(s) for "Color Experiments."
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Development and performance simulations of a soft X‐ray and XUV split‐and‐delay unit at beamlines FL23/24 at FLASH2 for time‐resolved two‐color pump–probe experiments
The split‐and‐delay unit (SDU) at FLASH2 will be upgraded to enable the simultaneous operation of two temporally, spatially and spectrally separated probe beams when the free‐electron laser undulators are operated in a two‐color scheme. By means of suitable thin filters and an optical grating beam path a wide range of combinations of photon energies in the spectral range from 150 eV to 780 eV can be chosen. In this paper, simulations of the spectral transmission and performance parameters of the filter technique are discussed, along with a monochromator with dispersion compensation presently under construction. A set‐up for two‐color experiments at FLASH2 utilizing the split‐and‐delay unit at FL23 and FL24 is presented. It will be achieved by using suitable filters and an additional grating beam path with a dispersion compensated monochromator.
Radical Anions of Oxidized vs. Reduced Oxytocin: Influence of Disulfide Bridges on CID and Vacuum UV Photo-Fragmentation
The nonapeptide oxytocin (OT) is used as a model sulfur-containing peptide to study the damage induced by vacuum UV (VUV) radiations. In particular, the effect of the presence (or absence in reduced OT) of oxytocin’s internal disulfide bridge is evaluated in terms of photo-fragmentation yield and nature of the photo-fragments. Intact, as well as reduced, OT is studied as dianions and radical anions. Radical anions are prepared and photo-fragmented in two-color experiments (UV + VUV) in a linear ion trap. VUV photo-fragmentation patterns are analyzed and compared, and radical-induced mechanisms are proposed. The effect of VUV is principally to ionize but secondary fragmentation is also observed. This secondary fragmentation seems to be considerably enabled by the initial position of the radical on the molecule. In particular, the possibility to form a radical on free cysteines seems to increase the susceptibility to VUV fragmentation. Interestingly, disulfide bridges, which are fundamental for protein structure, could also be responsible for an increased resistance to ionizing radiations. Graphical Abstract
Experiments with light and color
This introduction to the power and versatility of light includes experiments in white light, the color spectrum, and how light changes direction when it passes from one medium to another.
Functional Identification of the Isopentenyl Diphosphate Isomerase Gene from Fritillaria unibracteata
Isopentenyl diphosphate isomerase (IPI) is a key enzyme in the synthesis of isoprenoids. In this paper, the in vivo biological activity of the IPI gene from Fritillaria unibracteata (FuIPI) was investigated. Combining a color complementation experiment with High-Performance Liquid Chromatography analysis showed that the FuIPI gene could accumulate β-carotene in Escherichia coli, and Glu190 was identified as a key residue for its catalytic activity. Bioinformatics analysis together with subcellular localization indicated that the FuIPI protein was localized in chloroplasts. Compared with wild-type Arabidopsis thaliana, FuIPI transgenic plants had higher abscisic acid content and strengthening tolerance to drought and salt stress. Overall, these results indicated that the FuIPI gene had substantial biological activity in vivo, hopefully laying a foundation for its further research and application in liliaceous ornamental and medicinal plants.
Delayed plumage maturation increases overwinter survival in North Island robins
Many bird species show delayed plumage maturation (DPM), retaining sub-adult plumage until after their first breeding season. Most explanations assume that DPM increases fitness over the breeding season. However, unless birds undergo a full moult before breeding, DPM could also be an adaptation to increase survival over the previous winter. The winter adaptation hypothesis has never been tested owing to the difficulty of measuring overwinter survival. We experimentally tested this hypothesis in North Island robins (Petroica longipes) using a closed island population where we could accurately estimate survival. The experiment involved dyeing 41 juveniles to mimic adult males, and comparing their survival with 41 control juveniles treated with the same peroxide base minus the pigment. The population was monitored with a series of resighting surveys, and mark-recapture analysis used to estimate overwinter survival. Survival probability was estimated to be 10% for dyed birds versus 61% for control birds in 2001, and 29% for dyed birds versus 40% for control birds in the winter of 2002, supporting the winter adaptation hypothesis for DPM. Access to suitable habitat is the key factor limiting juvenile survival in this population, and the locations where dyed juveniles were sighted suggest that they were often excluded from suitable areas.
Design of Gene Expression Microarray Experiments
This chapter contains sections titled: Introduction Gene Expression Microarray Technology Preprocessing of Microarray Fluorescence Intensities Introduction to Gene Expression Microarray Experimental Design Two‐Treatment Experiments Using Two‐Color Microarrays Two‐Color Microarray Experiments Involving More Than Two Treatments Multifactor Two‐Color Microarray Experiments Phase 2 Designs for Complex Phase 1 Designs References
Visual Color Quality Experiments at the Technische Universität Darmstadt
This chapter presents the method and the results of the color quality experiments of the authors' laboratory and reviews model equations for color quality for practical use. The presentation of the experiments follows a system that considers all relevant aspects for the lighting engineer with the motivation of understanding and modeling color quality as it is perceived and judged by the user of the light source in an interior lighting application. The chapter summarizes all semantic interpretation aspects and provides criterion values for all types of color quality metrics. First, it describes the semantic interpretation of color differences to interpret color fidelity indices, color discrimination measures, and to provide criterion values of white tone chromaticity for the binning of white LEDs. Second, the chapter also presents the semantic interpretation of the color appearance attributes modeled in a synoptic view of all three visual attributes, color preference, naturalness, and vividness.