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Copper ferrite−copper oxide (CuO-CuFe2O4) nanoparticles as a semiconductor composite with p–n junction were synthesized by co-precipitation reaction. Then, a novel CuO-CuFe2O4 carbon paste modified electrode was fabricated which displays an effectual electrocatalytic response to the oxidation of acetaminophen (AC) and codeine (CO). A linear range of 0.01–1.5 μmol L−1 and 0.06–10.0 μmol L−1 with the detection limits of 0.007 μmol L−1 and 0.01 μmol L−1 were achieved for AC and CO, respectively. The practical usage of the proposed sensor revealed reasonable results for quantification of AC and CO in biological fluids. [Display omitted] •Copper ferrite−copper oxide nanoparticles as a semiconductor composite with p–n junction were synthesized by co-precipitation reaction.•The structure and morphology of nanocomposite was characterized using XRD, FE-SEM and FT-IR spectra.•CuO-CuFe2O4 carbon paste modified electrode was fabricated which displays an effectual electrocatalytic response to the oxidation of acetaminophen and codeine.•No interference was seen from typical species existing in biological samples which suggest selectivity of the modified electrode for accurate analysis of AC and CO in biological samples.

Copper ferrite-copper oxide (CuO-CuFe O ) nanoparticles as a semiconductor composite with p-n junction were synthesized by co-precipitation reaction. Then, a novel CuO-CuFe O carbon paste modified electrode was fabricated which displays an effectual electrocatalytic response to the oxidation of acetaminophen (AC) and codeine (CO). A linear range of 0.01-1.5 μmol L and 0.06-10.0 μmol L with the detection limits of 0.007 μmol L and 0.01 μmol L were achieved for AC and CO, respectively. The practical usage of the proposed sensor revealed reasonable results for quantification of AC and CO in biological fluids.