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result(s) for
"Descriptive and experimental mechanics"
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Reproducible reformation of a bilayer lipid membrane using microair bubbles
by
Toshihisa Osaki
,
Sho Takamori
,
Shoji Takeuchi
in
Bubbles
,
Descriptive and experimental mechanics
,
Design
2023
Planar bilayer lipid membranes (BLMs) are widely used as models for cell membranes in various applications, including drug discovery and biosensors. However, the nanometer‐thick bilayer structure, assembled through hydrophobic interactions of amphiphilic lipid molecules, makes such BLM systems mechanically and electrically unstable. In this study, we developed a device to reform BLMs using a microair bubble. The device consists of a double well divided by a separator with a microaperture, where a BLM was formed by infusing a lipid‐dispersed solvent and an aqueous droplet into each well in series. When the BLM ruptured, a microair bubble was injected from the bottom of the well to split the merged aqueous droplet at the microaperture, which resulted in the reformation of two lipid monolayers on the split droplets. By bringing the two droplets into contact, a new BLM was formed. An angled step design was introduced in the BLM device to guide the bubble and ensure the splitting of the merged droplet. We also elucidated the optimal bubble inflow rate for the reproducible BLM reformation. Using a 4‐channel parallel device, we demonstrated the individual and repeatable reformation of BLMs. Our approach will aid the development of automated and arrayed BLM systems.
This paper describes the microbubble‐assisted formation of a planar bilayer lipid membrane. Self‐assembly of a lipid monolayer occurs on aqueous droplets in a lipid‐dispersed solvent, and a bilayer is formed at the interface of these two droplets. However, the 5‐nm‐thick bilayer structure makes it unstable, often leading to droplet fusion. The device uses an injected microbubble to split the merged droplet, reforming monolayers on the split droplets. By bringing them into contact, a new bilayer is formed. We demonstrate reproducible bilayer formation.
Journal Article
Numerical Study on an Interface Compression Method for the Volume of Fluid Approach
by
Yuria Okagaki
,
Masahiro Ishigaki
,
Taisuke Yonomoto
in
computational fluid dynamics (CFD)
,
Descriptive and experimental mechanics
,
QC120-168.85
2021
Many thermohydraulic issues about the safety of light water reactors are related to complicated two-phase flow phenomena. In these phenomena, computational fluid dynamics (CFD) analysis using the volume of fluid (VOF) method causes numerical diffusion generated by the first-order upwind scheme used in the convection term of the volume fraction equation. Thus, in this study, we focused on an interface compression (IC) method for such a VOF approach; this technique prevents numerical diffusion issues and maintains boundedness and conservation with negative diffusion. First, on a sufficiently high mesh resolution and without the IC method, the validation process was considered by comparing the amplitude growth of the interfacial wave between a two-dimensional gas sheet and a quiescent liquid using the linear theory. The disturbance growth rates were consistent with the linear theory, and the validation process was considered appropriate. Then, this validation process confirmed the effects of the IC method on numerical diffusion, and we derived the optimum value of the IC coefficient, which is the parameter that controls the numerical diffusion.
Journal Article
Shedding of Cavitation Clouds in an Orifice Nozzle
2024
Focused on the unsteady property of a cavitating water jet issuing from an orifice nozzle in a submerged condition, this paper presents a fundamental investigation of the periodicity of cloud shedding and the mechanism of cavitation cloud formation and release by combining the use of high-speed camera observation and flow simulation methods. The pattern of cavitation cloud shedding is evaluated by analyzing sequence images from a high-speed camera, and the mechanism of cloud formation and release is further examined by comparing the results of flow visualization and numerical simulation. It is revealed that one pair of ring-like clouds consisting of a leading cloud and a subsequent cloud is successively shed downstream, and this process is periodically repeated. The leading cloud is principally split by a shear vortex flow along the nozzle exit wall, and the subsequent cloud is detached by a re-entrant jet generated while a fully extended cavity breaks off. The subsequent cavitation cloud catches the leading one, and they coalesce over the range of x/d≈1.8~2.5. Cavitation clouds shed downstream from the nozzle at two dominant frequencies. The Strouhal number of the leading cavitation cloud shedding varies from 0.21 to 0.29, corresponding to the injection pressure. The mass flow rate coefficient fluctuates within the range of 0.59~0.66 at the same frequency as the leading cloud shedding under the effect of cavitation.
Journal Article
An Experimental Study on the Effect of Distance and Sheltering Area of a Group of Linearly Arranged Sacrificial Piles on Reducing Local Scour around a Circular Bridge Pier under Clear-Water Conditions
2024
One of the major problems associated with bridge piers is ensuring their safety against local scouring caused by the erosive action of flow. Numerous countermeasures have been developed and tested to solve this problem, among which sacrificial piles are highly recognized due to their high performance, economy, durability, and ease of construction. Several factors affect the performance of sacrificial piles, such as their number, size, degree of submergence, and geometric arrangement parameters. In this study, the performance of a group of linearly arranged cylindrical sacrificial piles in reducing local scour around a circular bridge pier was investigated by varying the number of piles (or sheltering area) and distance between piles and the pier under clear-water conditions. Three values of distance between piles and the pier and three values of sheltering area (or number of piles) were tested. The efficiencies of sacrificial piles in different configurations were presented in terms of the percentage reduction in maximum scour depth at an unprotected pier under the same hydraulic conditions. The results of this experiment show that when linearly arranged sacrificial piles are placed close to the pier (at distance D; D is the pier diameter), an increase in the number of piles (or sheltered area) results in an increased scour depth, and when placed far from the pier (2D and 3D), an increase in the number of piles results in a decrease in scour depth around the pier. In addition, for 40% and 60% sheltering conditions, scour depth increased with an increase in the spacing between piles and the pier, while for 80% sheltering conditions, optimum protection was observed at a distance of 2D. Overall, two piles placed at distance D provided optimum protection with a scour depth reduction of 41.6%, while minimum protection was recorded when the same were placed at a spacing of 3D from the pier (25.5%).
Journal Article
Computational Approach for the Fluid-Structure Interaction Design of Insect-Inspired Micro Flapping Wings
2022
A flight device for insect-inspired flapping wing nano air vehicles (FWNAVs), which consists of the micro wings, the actuator, and the transmission, can use the fluid-structure interaction (FSI) to create the characteristic motions of the flapping wings. This design will be essential for further miniaturization of FWNAVs, since it will reduce the mechanical and electrical complexities of the flight device. Computational approaches will be necessary for this biomimetic concept because of the complexity of the FSI. Hence, in this study, a computational approach for the FSI design of insect-inspired micro flapping wings is proposed. This approach consists of a direct numerical modeling of the strongly coupled FSI, the dynamic similarity framework, and the design window (DW) search. The present numerical examples demonstrated that the dynamic similarity framework works well to make different two FSI systems with the strong coupling dynamically similar to each other, and this framework works as the guideline for the systematic investigation of the effect of characteristic parameters on the FSI system. Finally, an insect-inspired micro flapping wing with the 2.5-dimensional structure was designed using the proposed approach such that it can create the lift sufficient to support the weight of small insects. The existing area of satisfactory design solutions or the DW increases the fabricability of this wing using micromachining techniques based on the photolithography in the micro-electro-mechanical systems (MEMS) technology. Hence, the proposed approach will contribute to the further miniaturization of FWNAVs.
Journal Article
Numerical Investigation of 3D Flow Properties around Finite Emergent Vegetation by Using the Two-Phase Volume of Fluid (VOF) Modeling Technique
by
Norio Tanaka
,
Amina
in
Air entrainment
,
Aspect ratio
,
aspect ratio (AR = vegetation width-length ratio)
2022
This study predicts how the Free Surface Level (FSL) variations around finite length vegetation affect flow structure by using a numerical simulation. The volume of fluid (VOF) technique with the Reynolds stress model (RSM) was used for the simulation. Multizone Hexahedral meshing was adopted to accurately track the free surface level with minimum numerical diffusion at the water–air interface. After the validation, finite length emergent vegetation patches were selected based on the aspect ratio (AR = vegetation width-length ratio) under constant subcritical flow conditions for an inland tsunami flow. The results showed that the generation of large vortices was predominated in wider vegetation patches (AR > 1) due to the increase and decrease in the FSL at the front and back of the vegetation compared to longer vegetation patches (AR ≤ 1), as this offered more resistance against the approaching flow. The wider vegetation patches (AR > 1) are favorable in terms of generating a large area of low velocity compared to the longer vegetation patch (AR < 1) directly downstream of the vegetation patch. On the other hand, it has a negative impact on the adjacent downstream gap region, where a 14.3–34.9% increase in velocity was observed. The longer vegetation patches (AR < 1) generate optimal conditions within the vegetation region due to great velocity reduction. Moreover, in all the AR vegetation cases, the water turbulent intensity was maximum in the vegetation region compared to the adjacent gap region and air turbulent intensity above the FSL, suggesting strong air entrainment over this region. The results of this study are important in constructing vegetation layouts based on the AR of the vegetation for tsunami mitigation.
Journal Article
Numerical Analysis of Non-Newtonian Fluid Effects on the Equilibrium Position of a Suspended Particle and Relative Viscosity in Two-Dimensional Flow
2024
A solvent in suspension often has non-Newtonian properties. To date, in order to determine these properties, many constitutive equations have been suggested. In particular, power-law fluid, which describes both dilatant and pseudoplastic fluids, has been used in many previous studies because of its simplicity. Then, the Herschel–Bulkley model is used, which describes fluid with yield stress. In this study, we considered how a non-Newtonian solvent affected the equilibrium position of a particle and relative viscosity using the regularized lattice Boltzmann method for fluid and a two-way coupling scheme for the particle. We focused on these methods so as to evaluate the non-Newtonian effects of a solvent. The equilibrium position in Bingham fluid was closer to the wall than that in Newtonian or power-law fluid. In contrast, the tendency of relative viscosity in Bingham fluid for each position was similar to that in power-law fluid.
Journal Article
Experimental Analysis of Local Condensation Heat Transfer Characteristics of CF3I Inside a Plate Heat Exchanger
2023
Due to its low global warming potential (GWP) and good environmental properties, CF3I can be a suitable component of refrigerant mixtures in the field of refrigeration and air conditioning. In this work, the local condensation heat transfer characteristics of CF3I were experimentally investigated in a plate heat exchanger (PHE). The condensation heat transfer experiments were carried out under conditions of vapor qualities from 1.0 to 0.0, at saturation temperatures of 25–30 °C, mass fluxes of 20–50 kg/m2s, and heat fluxes of 10.4–13.7 kW/m2. Local heat transfer coefficients were found to vary in both the horizontal and vertical directions of the plate heat exchanger showing similar trends in all mass fluxes. In addition, the characteristics of local heat flux and wall temperature distribution as a function of distance from the inlet to the outlet of the refrigerant channel were explored in detail. The comparison of the experimental data of CF3I with that of R1234yf in the same test facility showed that the heat transfer coefficients of CF3I were comparable to R1234yf at a low vapor quality and a mass flux of 20 kg/m2s. However, R1234yf exhibited a transfer coefficient about 1.5 times higher at all vapor qualities and a mass flux of 50 kg/m2s. The newly developed correlation predicts well the experimentally obtained data for both CF3I and R1234yf within ±30%.
Journal Article
Utilization of Geogrid and Water Cushion to Reduce the Impact of Nappe Flow and Scouring on the Downstream Side of a Levee
2022
Water overflowing from a levee generates scour holes on the toe, which progresses towards the backward crest of the levee and results in nappe flow generation. The direct collision of nappe flow on the downstream area causes levee failure. It is important to introduce a novel countermeasure against scouring caused by nappe flow. Hence, the present study utilized a new technique to reduce scouring due to nappe flow by introducing a combination of pooled water and geogrids. Herein, laboratory experiments were conducted with the three cases for rigid bed (R), named as NR, G1R, G2R (N, G1 and G2 represent no geogrid, geogrid 1 and geogrid 2, respectively), and moveable bed (M), named as NM (nothing moveable), G1M (geogrid 1 moveable), G2M (geogrid 2 moveable), to elucidate the effect of dimensionless pooled water depth (DP*), overtopping depth (DC*) and the aperture size of geogrids (d*) on flow structure and scouring. The results showed that the scour depth was reduced by around 17–31% during the NM cases, 57–78% during the G1M cases and 100% during the G2M cases by increasing the DP* from 0.3 to 0.45. Hence, the combination of geogrids with pooled water (G1M, G2M) performed a vital role in suppressing the scouring, but the results of G2M were more advantageous in terms of scouring countermeasures.
Journal Article