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Introducción: los estomas intestinales representan un impacto significativo en la calidad de vida de las personas; sin embargo, estos deben revertirse después de haberse restituido el tránsito intestinal o la resolución del proceso inflamatorio inicial. Por otro lado, la negación de la persona para su reversión puede deberse a la falta de información y orientación por parte de los profesionales de la salud. Por lo anterior es importante identificar las intervenciones de Enfermería en la atención de la persona con reversión del estoma intestinal. Materiales y métodos: se realizó una revisión integrativa de la literatura de alcance descriptivo en el período comprendido entre los años 2015 a 2020, a través de las bases de datos Wos, Pubmed, Scopus, Scielo y Cochrane. Se seleccionaron 36 artículos que cumplieron con los criterios de inclusión y exclusión con el respectivo análisis metodológico. Resultados: Se identificaron las siguientes intervenciones de Enfermería, para el preoperatorio: valoración preoperatoria, preparación intestinal y seguimiento a comorbilidades. El intraoperatorio: profilaxis, preparación de la piel, técnica quirúrgica y cierre de la pared abdominal. En el posoperatorio: cuidado de la herida quirúrgica, calidad de vida y educación. Discusión: es importante la reflexión sobre el tiempo de reversión, la técnica quirúrgica y la importancia de las intervenciones por Enfermería. Conclusión: Enfermería cumple un papel importante en la reversión del estoma, no solo por los cuidados físicos y la educación que se brinda, sino también en las intervenciones aplicables al contexto social y emocional que afectan el estilo de vida de la persona.

Objective: To evaluate the effect of a stoma nursing care program on the personal adjustment to an ostomy. Materials and methods: A quasi-experimental study with a control group. The sample of 105 patients with stoma was divided into two groups (intervention group and control group), with assessments in the first and sixth month after hospital discharge. Information was collected using the Elimination Ostomy Adjustment Scale. Student’s t-tests analysis were performed. Results: One month after hospital discharge, the adjustment to the stoma was satisfactory and similar (p > 0.05) in both groups, and in the sixth month, statistically more favorable in the intervention group (p < 0.001); a statistically significant difference between the two assessments (p < 0.001) is found in the intervention group only. Conclusions: The intervention program has a positive influence on the adjustment to the stoma, and its contribution is significant six months after hospital discharge. Systematic interventions of an ostomy care specialized nurse have shown positive effects on ostomy adjustment, which is recommended since the preoperative period.

It is well known that endogenous abscisic acid (ABA) levels increase rapidly in response to drought stress and that this induces stomatal closure. In Arabidopsis thaliana, ABA levels increased rapidly in the leaves and roots when intact wild-type whole plants were exposed to drought stress. However, if the leaves and roots were separated and exposed to drought independently, the ABA level increased only in the leaves. These results suggest that, under our experimental conditions, ABA is synthesized mainly in the leaves in response to drought stress and that some of the ABA accumulated in the leaves is transported to the roots. Tracer experiments using isotope-labeled ABA indicate that the movement of ABA from leaves to roots is activated by water deficit in the roots. We also demonstrate that the endogenous ABA level in the leaves increased only when the leaves themselves were exposed to drought stress, suggesting that leaves play a major role in the production of ABA in response to acute water shortage.

▪ Abstract  The plant hormone abscisic acid (ABA) plays a major role in seed maturation and germination, as well as in adaptation to abiotic environmental stresses. ABA promotes stomatal closure by rapidly altering ion fluxes in guard cells. Other ABA actions involve modifications of gene expression, and the analysis of ABA-responsive promoters has revealed a diversity of potential cis-acting regulatory elements. The nature of the ABA receptor(s) remains unknown. In contrast, combined biophysical, genetic, and molecular approaches have led to considerable progress in the characterization of more downstream signaling elements. In particular, substantial evidence points to the importance of reversible protein phosphorylation and modifications of cytosolic calcium levels and pH as intermediates in ABA signal transduction. Exciting advances are being made in reassembling individual components into minimal ABA signaling cascades at the single-cell level.

BackgroundGomphrena perennis (Gpe) and G. pulchella (Gpu) are perennial glyphosate-tolerant amaranthaceous weeds of central and northern Argentina where glyphosate-resistant soybeans are grown with no-till. The study of weeds leaves morphoanatomy is important to understand environmental adaptation and could be used to explain herbicide absorption. Leaf surfaces of Gomphrena species were previously described for phylogenetic studies, and morphoanatomy of two Gomphrena perennis populations was characterized for glyphosate tolerance. There are no reports on comparative studies of Gpe and Gpu. We hypothesize that these two species differ in their morpho anatomical characteristics.ObjectiveThe aim of this research was to characterize and compare their leaf morphoanatomy throughout their life cycle.MethodsFully developed leaves of the upper middle third were extracted from plants grown in pots in a greenhouse at three phenological stages: eight true leaves (S1), beginning of branching (S2), and full flowering (S3). In both leaf sides, densities of epidermal cells, stomata and trichomes, and ostiole lengths were quantified. In main and secondary bundle anatomic parameters were measured: thickness of total blade, upper and lower epidermis, and mesophyll, and sheath length.ResultsBoth species are amphiestomatic. Gpu had higher stomata densities in lower epidermis than Gpe at S1. Gpu had higher trichome densities than Gpe, whereas the latter presented higher values in anatomic variables mainly in secondary bundle (total blade and total mesophyll) at all stages.ConclusionsThere were no marked differences in the morphoanatomy between the two species throughout their life cycle.

Objectives: To characterize the national and international literature on the teaching of self-care to bowel ostomates and their relatives. Methods: It is an integrative review, in LILACS and MEDLINE electronic databases, in the period from 1996 to 2006, with the keywords 'teaching', 'ostomates' and 'nursing'. Results: The sample was composed of eight articles, which reported the importance of teaching strategies applied with patients and their families regarding self-care and management of the stoma and collectors, however, no study has brought specific and systematized teaching strategies. Conclusion: The analyzed literature has demonstrated the importance of teaching strategies addressing the issue of selfcare for the ostomates, but expressed the lack of researches and publications on the implementation of contextualized actions and with appropriate language for these patients and their families. Objetivos: Caracterizar a literatura nacional e internacional sobre o ensino do autocuidado a pacientes estomizados intestinais e seus familiares. Métodos: Trata-se de uma revisão integrativa nas bases de dados eletrônicas LILACS e MEDLINE, no período de 1996 a 2006, com as palavras-chave \"ensino\", \"ostomizados\" e \"enfermagem\". Resultados: A amostra foi composta por oito artigos, os quais relataram a importância de estratégias de ensino aos pacientes e familiares sobre o autocuidado com o estoma e equipamentos coletores, porém, nenhum estudo trouxe estratégias de ensino específicas e sistematizadas. Conclusão: A literatura analisada demonstrou a importância das estratégias de ensino sobre o autocuidado do paciente estomizado, entretanto, expressou a escassez de pesquisas e publicações sobre a implementação de ações contextualizadas e com linguagem adequada aos pacientes e seus familiares. Objetivos: Caracterizar la literatura nacional e internacional sobre la enseñanza de autocuidado para pacientes ostomizados intestinales y a sus familias. Métodos: Se trata de una revisión integradora en las bases de datos electrónicas MEDLINE y LILACS en el periodo 1996-2006, utilizando las palabras clave \"escuela\", \"ostomizados\" y \"enfermería\". Resultados: La muestra estuvo compuesta por ocho artículos, los cuales mostraron la importancia de las estrategias de enseñanza a los pacientes y familiares sobre el autocuidado con el orificio y el equipo de recolección, sin embargo, ningún estudio ha mostrado estrategias de enseñanza específicas y sistematizadas. Conclusión: La revisión de la literatura demostró la importancia de la enseñanza de estrategias de autocuidado en los pacientes ostomizados, sin embargo, mostró la falta de investigaciones y publicaciones sobre la implementación de acciones en el contexto y con un lenguaje apropiado para los pacientes y sus familias.

Plant aquaporins are believed to facilitate water transport across cell membranes. However, the relationship between aquaporins and drought resistance in plants remains unclear. VfPIP1, a putative aquaporin gene, was isolated from Vicia faba leaf epidermis, and its expression was induced by abscisic acid (ABA). Our results indicated that the VfPIP1 protein was localized in the plasma membrane, and its expression in V. faba was induced by 20% polyethylene glycol 6000. To further understand the function of VfPIP1, we obtained VfPIP1-expressing transgenic Arabidopsis thaliana plants under the control of the CaMV35S promoter. As compared to the wild-type control plants, the transgenic plants exhibited a faster growth rate, a lower transpiration rate, and greater drought tolerance. In addition, the stomata of the transgenic plants closed significantly faster than those of the control plants under ABA or dark treatment. These results suggest that VfPIP1 expression may improve drought resistance of the transgenic plants by promoting stomatal closure under drought stress.

Oscillations in cytosolic free Ca2+ concentration ([Ca2+]cyt) are an important component of Ca2+-based signal transduction pathways. This fact has led us to investigate whether oscillations in [Ca2+]cyt are involved in the response of stomatal guard cells to the plant hormone abscisic acid (ABA). We show that ABA induces oscillations in guard-cell [Ca2+]cyt. The pattern of the oscillations depended on the ABA concentration and correlated with the final stomatal aperture. We examined the mechanism by which ABA generates oscillations in guard-cell [Ca2+]cyt by using 1-(6-([17 beta-3-methoxyestra- 1,3,5(10)-trien-17-yl]amino)hexyl)-1H-pyrrole 2,5-dione (U-73122), an inhibitor of phosphoinositide-specific phospholipase C (PI-PLC)-dependent processes in animals. U-73122 inhibited the hydrolysis of phosphatidylinositol 4,5-bisphosphate by a recombinant PI-PLC, isolated from a guard-cell-enriched cDNA library, in a dose-dependent manner. This result confirms that U-73122 is an inhibitor of plant PI-PLC activity. U-73122 inhibited both ABA-induced oscillations in [Ca2+]cyt and stomatal closure. In contrast, U-73122 did not inhibit external Ca2+-induced oscillations in guard-cell [Ca2+]cyt and stomatal closure. Furthermore, there was no effect of the inactive analogue 1-(6-([17 beta- 3-methoxyestra-1,3,5(10)-trien-17-yl] amino)hexyl)-2,5-pyrrolidinedione on recombinant PI-PLC activity or ABA-induced and external Ca2+-induced oscillations in [Ca2+]cyt and stomatal closure. This lack of effect suggests that the effects of U-73122 in guard cells are the result of inhibition of PI-PLC and not a consequence of nonspecific effects. Taken together, our data suggest a role for PI-PLC in the generation of ABA-induced oscillations in [Ca2+]cyt and point toward the involvement of oscillations in [Ca2+]cyt in the maintenance of stomatal aperture by ABA

Three published estimates of the frequency of polyploidy in angiosperms (30 to 35 percent, 47 percent, and 70 to 80 percent) were tested by estimating the genome size of extinct woody angiosperms with the use of fossil guard cell size as a proxy for cellular DNA content. The inferred chromosome numbers of these extinct species suggest that seven to nine is the primitive haploid chromosome number of angiosperms and that most angiosperms (approximately 70 percent) have polyploidy in their history