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Background Austropotamobius torrentium is a freshwater crayfish species native to central and south-eastern Europe, with an intricate evolutionary history and the highest genetic diversity recorded in the northern-central Dinarides (NCD). Its populations are facing declines, both in number and size across its entire range. By extanding current knowledge on the genetic diversity of this species, we aim to assist conservation programmes. Multigene phylogenetic analyses were performed using different divergence time estimates based on mitochondrial and, for the first time, nuclear DNA markers on the largest data set analysed so far. In order to reassess taxonomic relationships within this species we applied several species delimitation methods and studied the meristic characters with the intention of finding features that would clearly separate stone crayfish belonging to different phylogroups. Results Our results confirmed the existence of high genetic diversity within A. torrentium, maintained in divergent phylogroups which have their own evolutionary dynamics. A new phylogroup in the Kordun region belonging to NCD has also been discovered. Due to the incongruence between implemented species delimitation approaches and the lack of any morphological characters conserved within lineages, we are of the opinion that phylogroups recovered on mitochondrial and nuclear DNA are cryptic subspecies and distinct evolutionary significant units. Conclusions Geographically and genetically isolated phylogroups represent the evolutionary legacy of A. torrentium and are highly relevant for conservation due to their evolutionary distinctiveness and restricted distribution.

Morphologically variable species complexes can present significant challenges to conservation when taxonomic boundaries, and therefore conservation units, are ambiguous. In such cases, the definition of Evolutionarily Significant Units (ESUs) based on high-resolution genomic data can be an effective method to clarify genetic divergence and inform conservation actions. Here, we employ a population genomic approach using SNP data to delineate conservation units in a morphologically ambiguous plant species complex (Conospermum caeruleum; Proteaceae) from a global biodiversity hotspot. Over 200 individuals representing five subspecies and three informal morphological forms were sampled across a wide geographic range. Phylogenomic (ML tree and SplitsTree network) and population genomic (STRUCTURE, PCoA, FST) analyses resolved three divergent genetic groups that were incongruent with the current taxonomy, but consistent with geographic distribution. One ESU was comprised of three genetic subgroups, and these can be considered Management Units (MUs) to conserve population genetic structure and diversity. These MUs were surprisingly incongruent with the current subspecies-level taxonomy, but one MU was consistent with a recently documented morphological form, and all were consistent with ecogeographic distribution. We recommend a full taxonomic revision of the Conospermum genus based on a phylogenomic approach to assess any wider incongruence with morphology, and that conservation strategies should be informed by genetic data. Our study exemplifies the application of genomics to the conservation of a morphologically ambiguous species complex, enabling and enhancing the practical conservation of appropriate units of biodiversity.

Calculation of the cutoff probability in the Rand-ESU method. Identification of weak points of the method through the random sampling of spanning trees, also the Rand-ESU method with has implemented in the igraph library. Implemented in library igraph. Comparison of methods for counting subgraphs at four vertices. Prospects for the development of the spanning tree sampling method.

This article provides a new interpretation of the anonymous poem Moretum as erotic satire. Mindful of Shelley Haley's invitation to read it through a Black feminist lens, this article turns to recent Black feminist scholarship on pornography to argue that the presence of sex does not automatically negate the agency of Scybale, the African woman described in the poem as Simulus's custos . Further, I review the evidence for Simulus's own identity. Through a combination of Audre Lorde's Black queer lens and Paul Preciado's trans scholarship on the dildo, I further argue that by imagining Simulus as Black, queer, and/or trans, the power imbalance between Simulus and Scybale is greatly reduced. Lastly, I heed Haley's invitation to read Black protagonists of Latin poetry through Yoruba mythology, and turn to Henry Louis Gates Jr. to argue that Simulus's Blackness brings them closest to Esu, the genderqueer trickster god, which in turn helps us identify him with the author themselves.

An essential goal in conservation biology is delineating population units that maximize the probability of species persisting into the future and adapting to future environmental change. However, future‐facing conservation concerns are often addressed using retrospective patterns that could be irrelevant. We recommend a novel landscape genomics framework for delineating future “Geminate Evolutionary Units” (GEUs) in a focal species: (1) identify loci under environmental selection, (2) model and map adaptive conservation units that may spawn future lineages, (3) forecast relative selection pressures on each future lineage, and (4) estimate their fitness and likelihood of persistence using geo‐genomic simulations. Using this process, we delineated conservation units for the Yosemite toad (Anaxyrus canorus), a U.S. federally threatened species that is highly vulnerable to climate change. We used a genome‐wide dataset, redundancy analysis, and Bayesian association methods to identify 24 candidate loci responding to climatic selection (R2 ranging from 0.09 to 0.52), after controlling for demographic structure. Candidate loci included genes such as MAP3K5, involved in cellular response to environmental change. We then forecasted future genomic response to climate change using the multivariate machine learning algorithm Gradient Forests. Based on all available evidence, we found three GEUs in Yosemite National Park, reflecting contrasting adaptive optima: YF‐North (high winter snowpack with moderate summer rainfall), YF‐East (low to moderate snowpack with high summer rainfall), and YF‐Low‐Elevation (low snowpack and rainfall). Simulations under the RCP 8.5 climate change scenario suggest that the species will decline by 29% over 90 years, but the highly diverse YF‐East lineage will be least impacted for two reasons: (1) geographically it will be sheltered from the largest climatic selection pressures, and (2) its standing genetic diversity will promote a faster adaptive response. Our approach provides a comprehensive strategy for protecting imperiled non‐model species with genomic data alone and has wide applicability to other declining species.

The diversity on coral reefs has long captivated observers. We examine the mechanisms of speciation, role of ecology in speciation, and patterns of species distribution in a typical reef-associated clade—the diverse and colorful Calcinus hermit crabs—to address the origin of tropical marine diversity. We sequenced COI, 16S, and H3 gene regions for ∼90% of 56 putative species, including nine undescribed, \"cryptic\" taxa, and mapped their distributions. Speciation in Calcinus is largely peripatric at remote locations. Allopatric species pairs are younger than sympatric ones, and molecular clock analyses suggest that >2 million years are needed for secondary sympatry. Substantial niche conservatism is evident within clades, as well as a few major ecological shifts between sister species. Color patterns follow species boundaries and evolve rapidly, suggesting a role in species recognition. Most species prefer and several are restricted to oceanic areas, suggesting great dispersal abilities and giving rise to an ocean-centric diversity pattern. Calcinus diversity patterns are atypical in that the diversity peaks in the west-central oceanic Pacific rather than in the Indo-Malayan \"diversity center.\" Calcinus speciation patterns do not match well-worn models put forth to explain the origin of Indo-West Pacific diversity, but underscore the complexity of marine diversification.

State-of-charge balance is vital for allowing multiple energy storage units (ESUs) to make the most of stored energy and ensure safe operation. Concerning scenarios wherein boost converters are used as the interfaces between ESUs and loads, this paper proposes a balancing strategy for realizing consistent state-of-charge (SoC) levels and equal currents among different ESUs. This strategy is valid for both parallel and series applications. Its advantages also include its high precision of SoC equalization without extra sensors and fast convergence. A common outer voltage loop is used to accomplish tight voltage regulation, while multiple inner current loops are utilized to achieve current control and SoC balance simultaneously. Firstly, by introducing SoC-based current distribution ratios (CDRs) to modify current references online, the currents are gradually adjusted to eliminate SoC deviations. Secondly, to expedite the balancing process, current saturations are further adopted. Thirdly, the influences of accelerating factor and current limits in CDR expressions are analyzed, and their selection guidelines are subsequently provided. Fourthly, the controller design, consisting of a dual loop, is illustrated to guarantee sufficient stability margins. Fifthly, an experimental platform consisting of three battery ESUs is developed to verify the proposed strategy.

The Evolutionarily Significant Unit (ESU) was conceptualized in 1986 as a conservation unit below the species level, theoretically applicable to a wide range of taxa. The concept has gained support, and various definitions or criteria, some of which are inconsistent with each other, have since been proposed. Recent critiques of the ESU have pointed out the dominance of definitions biased to the identification of long-term isolation or neutral genetic variation, which has largely ignored the adaptive components. We present here the validity of such claims and show how the ESU definitions have actually been applied in research. We surveyed scientific journals for original papers supporting ESU designations and determined who among the proponents of ESU definitions have gained wider support. Our results indicate that indeed there are inconsistencies with the original concept and with the existing definitions. Although the original concept recommended both ecological and genetic data as the basis for identification of ESUs, which reflect true evolutionary variation, recent definitions have become biased to either neutral genetic variation or adaptive variation. The definition which uses genetic data to assess neutral genetic variation (long-term isolation) has gained major support, and therefore validates the earlier claims. To bridge the gap between the original concept and the practical application, we propose the use of partial ESU and full ESU designations. The application of full ESU should be limited solely to when both information about neutral genetic variation and adaptive variation are available. In other cases, in which only a part of the variation is examined, we should use the term partial ESU (e.g., molecular-based ESU) and continue to investigate focal populations from other aspects of variations to designate full ESU.

Currently, computerized systems, such as language learning, telephone advertising, criminal cases, computerized health care and education systems are rapidly spreading and creating an urgent need for improved productivity. Speech recordings are a rich source of personal, confidential data that can be used to support a wide variety of applications, from health profiling to biometric recognition. Therefore, it is important that the speech recordings are properly protected, so that they cannot be misused. The leakage of encrypted biometric information is irreversible and biometric links are renewable. The article proposes a block diagram of the identification of the users of the systems by individual voice characteristics, based on the joint use of the Deep Neural Network (DNN) method and $$i$$ i -vector in the model of the elementary speech units, distinguished by increased security from various types of attacks on the biometric identification system, which allowed identifying the users with probability of first and second errors genus 0.025 and 0.005. The analysis of the vulnerability of the modules of the biometric voice identification system was performed and a structural scheme of the voice identification system of the user identification by voice with enhanced the protection against attacks was proposed. The use of elementary speech units in the developed identification systems makes it possible to improve computational indicators, reduce subjective decisions in biometric systems, and increase the security against attacks on the voice biometric identification systems.

Conservation biologists have long debated the value of subspecies, which are morphologically and geographically identifiable but not necessarily evolutionarily distinctive. One example of a controversial subspecies is Cicindela formosa gibsoni, a tiger beetle that is nationally listed as threatened in Canada and whose taxonomic status is based primarily on its unique elytral (forewing) color pattern. To determine whether C. f. gibsoni represents one or more genetically distinctive units, we sampled 14 populations within or near this subspecies’ disjunct North American range and assessed their genetic differentiation from neighboring and phenotypically distinctive populations of C. f. formosa and C. f. fletcheri. Instead of clustering by color pattern, analyses of mitochondrial and nuclear markers recovered three geographically structured genetic groupings: a northern cluster from Canada, a southwestern cluster from northwestern Colorado, and a southeastern cluster of US populations east of the Continental Divide. These data, coupled with previously documented differences in larval morphology, suggest that populations of C. formosa in western Canada and northwestern Colorado may have independently evolved similar color patterns. Thus, we consider C. f. gibsoni to be endemic to Canada and describe the novel subspecies C. f. gaumeri ssp. nov. from northwestern Colorado. Both subspecies are evolutionarily significant units, and each deserves consideration for conservation listing. Collectively, our results reveal general congruence between mitochondrial and nuclear genetic data but conflict with color pattern, the conventional basis for subspecies designations in tiger beetles.