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The aim of this study was to assess how soil organic C (SOC) stocks and earthworm communities were modified in agroforestry systems compared to treeless control plots and within the agroforestry plots (tree rows vs alleys). We used a network of 13 silvoarable agroforestry sites in France along a north/south gradient. Total earthworm abundance and biomass were significantly higher in the tree rows than those in the control plots, but were not modified in the alleys compared to those in the control plots. Earthworm species richness, Shannon index, and species evenness were significantly higher in the tree rows than those in the alleys. Total abundance of epigeic, epi-anecic, strict anecic, and endogeic was higher in the tree rows. Surprisingly, earthworm individual weight was significantly lower in the tree rows than that in the alleys and in the control plots. SOC stocks were significantly higher in the tree rows compared to that in the control plots across all sites. Despite higher SOC stocks in the tree rows, the amount of available C per earthworm individual was lower compared to those in the control. The absence of disturbance (no tillage, no fertilizers, no pesticides) in the tree rows rather than increased SOC stocks therefore seems to be the main factor explaining the increased total abundance, biomass, and diversity of earthworms. The observed differences in earthworm communities between tree rows and alleys may lead to modified and spatially structured SOC dynamics within agroforestry plots.

The composting potential of two epigeic earthworms (P. excavatus and P. sansibaricus) was studied in 2002 to breakdown the domestic waste under laboratory conditions. The experimental container with P. sansibaricus showed maximum mineralization and decomposition rate than that of P. excavatus. Except for exchangeable K (it was higher (P = 0.004) in a container with P. excavatus), the domestic waste processed by P. sansibaricus showed about 6% more total nitrogen (P = 0.002) and about 7% more available P (P = 0.269) at the end than by P. excavatus. As compared with the initial level organic C content as well as C:N ratio showed a considerable reduction that was noted higher in substrate with P. sansibaricus than those by P. excavatus (organic C, t-test: P = 0.870; C:N ratio, t-test: P = 0.002). The growth (biomass increase) and reproduction parameters such as mean individual live weight, maximum individual growth rate (mg wt./worm/day), number of cocoons and reproduction rate (cocoon/worm/day) were higher in bedding with P. sansibaricus. The maximum earthworm mortality was in vermibed having P. sansibaricus (~50% higher than by P. excavatus) (t-test: P = 0.423), since both species did not show a drastic difference in waste mineralization rate, but comparatively, P. excavatus exhibited better growth and reproduction performance, which further support the suitability of the species for large scale vermiculture operations.

Global environmental change substantially affects soil detritivores, including earthworms, impacting host-microbiota interactions and altering key soil biogeochemical processes such as litter decomposition. As microbial communities are inherently capable of rapid evolution, responses of earthworms and associated microbiota (i.e., earthworm holobionts) to global environmental change may likely involve the interplay of ecological and evolutionary processes and feedbacks. Although species-level responses of earthworms to global environmental change are well-studied, the potential ecological and evolutionary responses of earthworm holobionts to environmental change remain unexplored. Here, we provide a conceptual framework to elaborate on the complex network of earthworm host−microbiota interactions that modify their traits in response to global environmental change, jointly shaping their ecology and evolution. Based on literature, we synthesize evidence of global environmental change impacts on earthworm host-microbiota and discuss evidence of their ecological and evolutionary responses to environmental change. Lastly, we highlight the agro- and eco-system level consequences of environmental change-mediated shift in earthworm host-microbiota functions. Soil legacies of environmental change have cascading detrimental impacts on the abundance, diversity, and functional dynamics of earthworm host-microbiota interactions in agriculture and ecosystems. The primary mechanisms driving such responses of earthworm hosts and associated microbial communities to environmental change include altered litter quality and host dietary preferences, competitive interactions and exclusion, habitat homogenization, and a shift in soil physicochemical and biological processes. Therefore, advancing knowledge of the intricate animal-microorganism interactions is crucial for belowground biodiversity management in a changing global environment.

Background and Aims: Organic farmers are showing increasing interest in using conservation tillage to improve the biological activities of soils. Here, we assessed whether conservation tillage in organic farming improves earthworm populations, root growth and soil physical quality in a sandy loam after 16 years of experiment. Methods: We compared the effect of a tillage gradient, with of two non-ploughed treatments (superficial tillage [ST] at 15 cm; very superficial tillage [VST] at 5–7 cm) and two ploughed treatments (moldboard ploughing [MP] at 30 cm; shallow moldboard ploughing [SMP] at 20 cm). Soil clod types, penetration resistance, abundance and activity of earthworms, root traits and biomass were assessed. Results: VST decreased soil compaction in topsoil (0 to 10 cm) compared to ploughed treatments (MP and SMP), but led to more compacted soil at 15 to 30 cm. Earthworm biomass (especially anecic) was higher under VST compared to MP and SMP and their galleries were better connected to the soil surface. However, there was no significant difference in the total volume of pores or diameter of galleries between 0 and 30 cm. Soil compaction in the non-ploughed treatments affected root traits, especially under VST, with lower specific root length, higher root diameter, and lower root tip elongation compared to MP and SMP. Conclusion: Biological activity did not compensate for the compaction of a sandy soil after 16 years without ploughing in organic farming. A more integrated approach (i.e. considering all 5 soil health principles) is needed to sustain soil health and functions, and meet current expectations about “ecological intensification”.

Two million tonnes of pesticides are currently used globally to improve crop production, yet these pesticides induce adverse effects on soil quality and biodiversity. Moreover, less than 1% of pesticides reach the target pests, while the rest contaminate the surrounding environment. In particular, earthworms are exposed to pesticides and are thus an ideal biological model for ecotoxicological research. Here, we review acute and sub-lethal effects of pesticides on earthworms. We found that pesticides alter growth, reproduction, behaviour, essential enzymes, and DNA of earthworms, even at low pesticide concentrations. These sub-lethal biomarkers allow to evaluate the overall response of an earthworm to pesticides, and to identify the risk for other non-target organisms.

Suppressor of cytokine signaling 2 (SOCS2), an E3 ubiquitin ligase, regulates the JAK/STAT signaling pathway, essential for cytokine signaling and immune responses. Its dysregulation contributes to cardiovascular diseases (CVDs) by promoting abnormal cell growth, inflammation, and resistance to cell death. This study aimed to elucidate the molecular mechanisms underlying the interactions between Lumbricus-derived proteins and peptides and SOCS2, with a focus on identifying potential therapeutic candidates for CVDs. Utilizing a multifaceted approach, advanced computational methodologies, including 3D structure modeling, protein–protein docking, 100 ns molecular dynamics (MD) simulations, and MM/PBSA calculations, were employed to assess the binding affinities and functional implications of Lumbricus-derived proteins on SOCS2 activity. The findings revealed that certain proteins, such as Lumbricin, Chemoattractive glycoprotein ES20, and Lumbrokinase-7T1, exhibited similar activities to standard antagonists in modulating SOCS2 activity. Furthermore, MM/PBSA calculations were employed to assess the binding free energies of these proteins with SOCS2. Specifically, Lumbricin exhibited an average ΔGbinding of −59.25 kcal/mol, Chemoattractive glycoprotein ES20 showed −55.02 kcal/mol, and Lumbrokinase-7T1 displayed −69.28 kcal/mol. These values suggest strong binding affinities between these proteins and SOCS2, reinforcing their potential therapeutic efficacy in cardiovascular diseases. Further in vitro and animal studies are recommended to validate these findings and explore broader applications of Lumbricus-derived proteins.

Background and aims Phytoremediation is an environment friendly, sustainable, and aesthetically pleasing technology for remediating heavy metal polluted soil. Earthworms are ubiquitous macrofauna in the soil ecosystem that play an important role in maintaining soil health and fertility. However, the understanding of earthworms' effect on phytoremediation remains limited. Methods In a greenhouse experiment, Lolium multiflorum was subjected to three levels of cadmium (0, 20, or 100 mg kg −1 ) fully crossed with two levels of earthworm treatments (i.e., with or without Eisenia foetida Savigny). Results Plant growth was inhibited while the root-shoot ratio and nitrogen accumulation in shoots were increased under 100 mg kg −1 cadmium. Earthworms interacted with cadmium level to affect the total phosphorus content in soil. Furthermore, earthworms enriched specific microorganisms and significantly influenced bacterial communities under 0 and 20 mg kg −1 cadmium. We observed a significant enrichment of specific microbial species in cadmium polluted soil when earthworms were present. Earthworms’ presence increased the sensitivity of fungal communities in soils polluted with cadmium. Conclusions Both earthworms and cadmium had certain impacts on the growth of plants, soil properties and microbial communities in root-zone soil. Moreover, the results suggest that earthworms may alleviate some negative effects of cadmium on soil microorganisms. The findings highlight the effect of earthworm on plant performance, soil properties, and root-zone microbial communities under cadmium stress, providing valuable insights into its role in phytoremediation of soils polluted with metals.

Windbreaks are among the most widespread agroforestry systems in the temperate biome. Tree windbreaks are expected to have unique soil properties and biotic communities, compared to adjacent agricultural fields. We compared key soil properties and communities of earthworms, bacteria and fungi along an agricultural transect through a tree windbreak into a cropped field. Soil properties, earthworm abundance and diversity, and bacterial and fungal alpha-diversity (16S-rRNA and ITS gene sequencing) were assessed along transects at distances of 0 (directly under the tree canopy), 8 (ecotone in the cropped field) and 50 m from the tree row in six mature (20- to 70-years-old) tree windbreaks located in southeastern Québec, Canada. Soil organic C, total N and C:N ratio were higher, while soil pH was lower in the tree row than in the adjacent cropped fields at distances of 8 m and 50 m from the windbreak. Each group of biota had a distinct pattern along the agricultural transect. Earthworm abundance and biomass were greater at 50 m than 8 m from the windbreak. Epigeic, endogeic, and anecic earthworms varied similarly along the agricultural transect. Fungal diversity was higher at 8 m than at 0 m from the windbreak. Bacterial diversity was higher at 50 m than at 0 m from the windbreak. Tree windbreaks made the soil environment in the ecotone more favorable for fungal communities, but less favorable for earthworm communities. Greater bacterial diversity was associated with agricultural activities than windbreaks.

AimsThis study was aimed to investigate the effect of earthworm Amynthas morrisi on the metal bioaccumulation by two plant species, Sedum alfredii Hance (Sedum) and maize (Zea mays L.), in a co-cropping system and possible influencing pathways, and assess the remediation potential of all combination of these organisms to identify the best option.MethodsIn this study, an eight-week microcosm experiment was conducted to investigate the main and interactive effects of the earthworm Amynthas morrisi, the hyperaccumulator plant Sedum alfredii Hance (Sedum), and maize (Zea mays L.) on C and N forms, and microbial characteristics and diethylene triamine penta acetic acid (DTPA) extractable metals of a soil heavily contaminated by multiple metals (i.e., Cd, Zn, Pb, and Cu). In addition, plant growth and metal accumulation were evaluated and the possible influencing pathways of metal accumulation by the two plant species were assessed. Finally, a remediation strategy was proposed based on the amounts of metals removed by sedum and maize.ResultsThe soil quality index achieved after eight weeks of experiments, was best with Sedum, and worst in the control with no plants and no earthworms. A path analysis suggests that earthworms exerted strong effects on plant metal accumulation by changing plant growth, with soil microbes playing a mediating role. The association of Sedum and Maize significantly increased the Zn concentration and decreased the Pb and Cu concentrations in the aboveground parts of Sedum compared with Sedum alone, whereas Sedum decreased the Zn, Cd, Pb, and Cu concentrations in the aboveground parts of maize compared with maize alone treatment. PCA showed that when maize was co-cropped with Sedum, metal transfer from its roots to the aboveground parts decreased.ConclusionsThe Earthworm + Sedum + Maize treatment displayed the most effective Cd and Zn removal, indicating that the combined introduction of A. morrisi and S. alfredii can effectively remediate soils co-contaminated by Cd and Zn in maize cropping systems.

Earthworms are fascinating animals capable of crawling and burrowing through various terrains using peristaltic motion and the directional friction response of their epidermis. Anisotropic anchoring governed by tiny appendages on their skin called setae is known to enhance the earthworm's locomotion. A multi‐material fabrication technique is employed to produce soft skins with bristles inspired by the earthworm epidermis and their setae. The effect of bristles arranged in triangular and square grids at two spatial densities on the locomotion capability of a simple soft crawling robot comprised of an extending soft actuator covered by the soft skin is investigated experimentally. The results suggest that the presence of bristles results in a rostral to caudal friction ratio of µR/µC > 1 with some variations across bristle arrangements and applied elongations. Doubling the number of bristles increases the robot's speed by a factor of 1.78 for the triangular grid while it is less pronounced for the rectangular grid with a small factor of 1.06. Additionally, it is observed that increasing the actuation stroke for the skin with the high‐density triangular grid, from 15% to 30%, elevates the speed from 0.5 to 0.9 mm s−1, but further increases in stroke to 45% may compromise the durability of the actuators with less gains in speed (1 mm s−1). Finally, it is demonstrated that a crawling robot equipped with soft skin can traverse both a linear and a curved channel. Earthworm‐inspired modular artificial skins are integrated into a soft linear actuator to investigate the effect of friction anisotropy on limbless locomotion. The skins feature stiff bristles in different arrangements on a soft shell fabricated using multi‐material silicone casting. Comprehensive experiments reveal the role of bristle arrangements and density, stroke, and actuator geometry on the robot's locomotion speed.