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An essential introduction to cardiac electrophysiology
This book provides undergraduate and postgraduate students with an accessible and comprehensive overview of the fascinating area of cardiac electrophysiology. Based on lectures presented to intercalating BSc medical students, it has been designed with the undergraduate in mind, but offers enough scope to be worthwhile at the postgraduate level.
Book
Robots take over electrophysiology
Image-guided targeting of fluorescently labeled neurons enables automated electrophysiological recordings of defined neuronal cell types.
Journal Article
Determination of Cell Capacitance Using the Exact Empirical Solution of ∂Y/∂Cm and Its Phase Angle
Measures of membrane capacitance offer insight into a variety of cellular processes. Unfortunately, popular methodologies rely on model simplifications that sensitize them to interference from inevitable changes in resistive components of the traditional cell-clamp model. Here I report on a novel method to measure membrane capacitance that disposes of the usual simplifications and assumptions, yet is immune to such interference and works on the millisecond timescale. It is based on the exact empirical determination of the elusive partial derivative, ∂Y/∂Cm, which heretofore had been approximated. Furthermore, I illustrate how this method extends to the vesicle fusion problem by permitting the determination of ∂Yv/∂Cv, thereby providing estimates of fusion pore conductance and vesicle capacitance. Finally, I provide simulation examples and physiological examples of how the method can be used to study processes that are routinely interrogated by measures of membrane capacitance.
Journal Article