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Cancer cells disseminate and seed in distant organs, where they can remain dormant for many years before forming clinically detectable metastases. Here we studied how disseminated tumor cells sense and remodel the extracellular matrix (ECM) to sustain dormancy. ECM proteomics revealed that dormant cancer cells assemble a type III collagen-enriched ECM niche. Tumor-derived type III collagen is required to sustain tumor dormancy, as its disruption restores tumor cell proliferation through DDR1-mediated STAT1 signaling. Second-harmonic generation two-photon microscopy further revealed that the dormancy-to-reactivation transition is accompanied by changes in type III collagen architecture and abundance. Analysis of clinical samples revealed that type III collagen levels were increased in tumors from patients with lymph node-negative head and neck squamous cell carcinoma compared to patients who were positive for lymph node colonization. Our data support the idea that the manipulation of these mechanisms could serve as a barrier to metastasis through disseminated tumor cell dormancy induction.

For perennial plants, bud dormancy is a crucial step as its progression over winter determines the quality of bud break, flowering, and fruiting. In the past decades, many studies, based on metabolic, physiological, subcellular, genetic, and genomic analyses, have unraveled mechanisms underlying bud dormancy progression. Overall, all the pathways identified are interconnected in a very complex manner. Here, we review early and recent findings on the dormancy processes in buds of temperate fruit trees species including hormonal signaling, the role of plasma membrane, carbohydrate metabolism, mitochondrial respiration and oxidative stress, with an effort to link them together and emphasize the central role of reactive oxygen species accumulation in the control of dormancy progression.

The development of an efficient, safe, and environment-friendly technique to terminate tuber dormancy in potatoes ( Solanum tuberosum L.) is of great concern due to the immense scope of multiple cropping all over the globe. The breakage of tuber dormancy has been associated with numerous physiological changes, including a decline in the level of starch and an increase in the levels of sugars during storage of freshly harvested seed potatoes, although their consistency across genotypes and various dormancy-breaking techniques have not yet been fully elucidated. The purpose of the present research is to assess the efficacy of four different dormancy-breaking techniques, such as soaking in 90, 60, or 30 mg L −1 solutions of benzyl amino purine (BAP) and 30, 20, or 10 mg L −1 gibberellic acid (GA3) alone and in the combination of optimized concentrations; cold pre-treatment at 6, 4, or 2 °C; electric shock at 80, 60, 40, or 20 Vs; and irradiation at 3.5, 3, 2.5, 2, 1.5, or 1 kGy on the tuber dormancy period and sprout length of six genotypes. Furthermore, the changes that occurred in tuber weight and endogenous starch, sucrose, fructose, and glucose contents in experimental genotypes following the application of these techniques were also examined. Overall, the most effective technique to terminate tuber dormancy and hasten spout growth was the combined application of BAP and GA 3 , which reduced the length of dormancy by 9.6 days compared to the untreated control, following 6.7 days of electric current, 4.4 days of cold pre-treatment, and finally irradiation (3.3 days). The 60 mg L −1 solution of BAP greatly reduced the dormancy period in all genotypes but did not affect the sprout length at all. The genotypes showed a weak negative correlation (r =  − 0.4) ( P  < 0.05) of endogenous starch contents with dormancy breakage and weight loss or a moderate (r =  − 0.5) correlation with sprout length, but a strong positive correlation (r = 0.8) of tuber glucose, fructose, and sucrose contents with dormancy breakage and weight loss. During 3 weeks of storage, sprouting commencement and significant weight loss occurred as tuber dormancy advanced towards breakage due to a reduction in starch and an increase in the sucrose, fructose, and glucose contents of the tubers. These findings could be advantageous for postponing or accelerating seed potato storage as well as investigating related physiological research in the future.

The post-harvest phase of potato tuber dormancy and sprouting are essential in determining the economic value. The intricate transition from dormancy to active growth is influenced by multiple factors, including environmental factors, carbohydrate metabolism, and hormonal regulation. Well-established environmental factors such as temperature, humidity, and light play pivotal roles in these processes. However, recent research has expanded our understanding to encompass other novel influences such as magnetic fields, cold plasma treatment, and UV-C irradiation. Hormones like abscisic acid (ABA), gibberellic acid (GA), cytokinins (CK), auxin, and ethylene (ETH) act as crucial messengers, while brassinosteroids (BRs) have emerged as key modulators of potato tuber sprouting. In addition, jasmonates (JAs), strigolactones (SLs), and salicylic acid (SA) also regulate potato dormancy and sprouting. This review article delves into the intricate study of potato dormancy and sprouting, emphasizing the impact of environmental conditions, carbohydrate metabolism, and hormonal regulation. It explores how various environmental factors affect dormancy and sprouting processes. Additionally, it highlights the role of carbohydrates in potato tuber sprouting and the intricate hormonal interplay, particularly the role of BRs. This review underscores the complexity of these interactions and their importance in optimizing potato dormancy and sprouting for agricultural practices.

Development of an efficient and eco-friendly technique to break tuber dormancy in potato ( Solanum tuberosum L.) is highly demanded due to the production of two or more crops annually. Several physiological and hormonal changes have been found to be related to the breaking of tuber dormancy; however, their consistency with genotypes and different protocols have not been well clarified. This study aims to evaluate the effectiveness of four dormancy-breaking methods, that is, plant growth regulator (PGR) dipping in 30, 60, or 90 mgL −1 benzyl amino purine (BAP) and 10, 20, or 30 mgL −1 gibberellic acids (GA3) alone and in the combination of optimized concentrations; electric current application at 20, 40, 60, or 80 Vs; cold pre-treatment at 2, 4, or 6 °C; irradiation at 1, 1.5, 2, 2.5, 3, or 3.5 kGy. In addition, changes in endogenous levels of abscisic acid (ABA), zeatin (ZT), and gibberellin A 1 (GA 1 ) in six potato genotypes after subjecting to these methods were investigated. Overall, the highest effective method for dormancy duration was the PGR application which shortened the duration by 18 days, followed by electric current (13 days), cold pre-treatment (9 days), and then irradiation (7 days). The solution of 60 mgL −1 BAP significantly reduced the dormancy duration in all genotypes but did not have a significant effect on the sprout length. While 20 mgL −1 GA 3 produced maximum sprout length with a non-significant effect on dormancy duration. The genotype × PGR interaction for dormancy duration was more pronounced in short- and medium-term dormancy genotypes than in long-term dormancy genotypes. The genotypes displayed a significant positive correlation between dormancy duration and ABA levels but exhibited a negative correlation between dormancy duration and ZT as well as GA 1 levels. From the first to the third week of storage, ABA was decreased in tubers while, however, ZT and GA 1 were increased. The obtained results could be useful for the postharvest storage of potato tuber and the related field of physiological investigation in future.

Class actions play an important role in civil litigation, but they suffer from a serious problem. No matter how many plaintiffs arc in the class, the individual claims are often miniscule, which can make distributing the inevitable settlement futile. Very few people make a claim, and it often costs more to mail the check than the check is worth. To solve this problem, Steven Shepherd proposed importing the cy pres doctrine (a trusts and estates device that allows courts to rewrite an unenforceable trust) into the class actions context. Shepherds framework instructs courts to devise the next best alternative for distributing the funds from a class action settlement. Oftentimes, this will involve donating the money to a charity whose work aligns with the policy goals of the statute creating the cause of action. Courts across the country have adopted this framework with open arms, and the doctrine has developed a mind of its own. Courts are now bending over backwards to approve these cy pres settlements, often overlooking clear legal errors, glaring public policy concerns, and flagrant ethical violations to justify them. But there is a more fundamental problem: cy pres is unconstitutional. Rule number one of federal jurisdiction is that plaintiffs must satisfy the irreducible constitutional minimum of Article III standing to sue. Plaintiffs lack standing when the requested remedy would not redress the harm, and cy pres settlements do not redress the harm the class has suffered. This Note argues courts should replace cy pres with an escheatment scheme that ensures class actions redress the plaintiffs harms. Escheatment serves the twin goals of both ensuring the class members receive their money (thereby satisfying the redressability requirement) while also preventing the egregious misconduct many attorneys engage in while negotiating these settlements.

Growth-regulating factors (GRFs) are transcription factors that play a pivotal role in plant growth and development. This study identifies 12 Solanum tuberosum GRF transcription factors (StGRFs) and analyzes their physicochemical properties, phylogenetic relationships, gene structures and gene expression patterns using bioinformatics. The StGRFs exhibit a length range of 266 to 599 amino acids, with a molecular weight of 26.02 to 64.52 kDa. The majority of StGRFs possess three introns. The promoter regions contain a plethora of cis -acting elements related to plant growth and development, as well as environmental stress and hormone response. All the members of the StGRF family contain conserved WRC and QLQ domains, with the sequences of these two conserved domain modules exhibiting high levels of conservation. Transcriptomic data indicates that StGRFs play a significant role in the growth and development of stamens, roots, young tubers, and other tissues or organs in potatoes. Furthermore, a few StGRFs exhibit differential expression patterns in response to Phytophthora infestans , chemical elicitors, heat, salt, and drought stresses, as well as multiple hormone treatments. The results of the expression analysis indicate that StGRF1 , StGRF2 , StGRF5 , StGRF7 , StGRF10 and StGRF12 are involved in the process of tuber sprouting, while StGRF4 and StGRF9 may play a role in tuber dormancy. These findings offer valuable insights that can be used to investigate the roles of StGRFs during potato tuber dormancy and sprouting.

During autumn perennial trees cease growth and form structures called buds in order to protect meristems from the unfavorable environmental conditions, including low temperature and desiccation. In addition to increased tolerance to these abiotic stresses, reproductive buds modulate developmental programs leading to dormancy induction to avoid premature growth resumption, and flowering pathways. Stress tolerance, dormancy, and flowering processes are thus physically and temporarily restricted to a bud, and consequently forced to interact at the regulatory level. We review recent genomic, genetic, and molecular contributions to the knowledge of these three processes in trees, highlighting the role of epigenetic modifications, phytohormones, and common regulatory factors. Finally, we emphasize the utility of transcriptomic approaches for the identification of key structural and regulatory genes involved in bud processes, illustrated with our own experience using peach as a model.

Bud dormancy in temperate perennials ensures the survival of growing meristems under the harsh environmental conditions of autumn and winter, and facilitates an optimal growth and development resumption in the spring. Although the molecular pathways controlling the dormancy process are still unclear, DORMANCY-ASSOCIATED MADS-BOX genes ( DAM ) have emerged as key regulators of the dormancy cycle in different species. In the present study, we have characterized the orthologs of DAM genes in European plum ( Prunus domestica L.). Their expression patterns together with sequence similarities are consistent with a role of PdoDAM s in dormancy maintenance mechanisms in European plum. Furthermore, other genes related to dormancy, flowering, and stress response have been identified in order to obtain a molecular framework of these three different processes taking place within the dormant flower bud in this species. This research provides a set of candidate genes to be genetically modified in future research, in order to better understand dormancy regulation in perennial species.

Dormancy is a mechanism for perennial trees to resist unfavourable environmental conditions. However, the molecular mechanisms underlying dormancy in apricots have not been fully characterized. Here, we investigated the content of the abscisic acid (ABA) in floral buds, which exhibited a decreasing trend from paradormancy to ecodormancy stages (D1–D3). Combining mRNA and miRNA analyses, 1458 miRNAs corresponding to 28,501 target genes were identified at three stages. The results of DEGs and DE miRNAs showed that 38 DEGs and 10 DE miRNAs responded to low temperatures and short photoperiods were found involved in maintaining the dormancy, and four genes ( PaNCED2 , PaCYP707A4.1 , PaPYL , and PaABF1 ) and miR5776-x involved in ABA biosynthesis and signaling pathways. Based on weighted gene co-expression network analysis (WGCNA), PaABF1 was identified as the key factor maintaining the dormancy. The findings provide new molecular mechanisms for further research of regulation of dormancy in apricot.