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To ensure food security and nutritional quality for a growing world population in the face of climate change, stagnant capture fisheries production, increasing aquaculture production and competition for natural resources, countries must be accountable for what they consume rather than what they produce. To investigate the sustainability of seafood consumption, we propose a methodology to examine the impact of seafood supply chains across national boundaries: the seafood consumption footprint. The seafood consumption footprint is expressed as the biomass of domestic and imported seafood production required to satisfy national seafood consumption, and is estimated using a multi-regional input output model. Thus, we reconstruct for the first time the global fish biomass flows in national supply chains to estimate consumption footprints at the global, country and sector levels (capture fisheries, aquaculture, distribution and processing, and reduction into fishmeal and fish oil) taking into account the biomass supply from beyond national borders.

Pumpkin seeds (Cucurbita pepo) and their derivatives have certain beneficial effects for farmed fish. Because of its great adaptability in diet, it is being investigated as a valuable option for commercial aquaculture feed in Asia. A trial study was performed to check the effects of pumpkin seed meal on rohu (Labeo rohita) fingerlings. This study aimed to investigate the effects of replacing the diet of L. rohita with fish meal (FM) and pumpkin seed meal (PSM) on the growth rate, carcass composition, and hematological profile. Six isonitrogenous and isocaloric meals (PSM-0%, PSM-15%, PSM-30%, PSM-45%, PSM 60%, and PSM-75%) were used in the experiment. Fingerlings of L. rohita were divided into three groups and fed for 80 days with PSM-based diets at 4% of their body weight. The group fed with a 45% PSM-based diet shows the maximum growth performance showing 21.23g weight gain, 383% weight gain %, 1.75 SGR and 98% survival rate. The fish fingerlings fed with the 45% PSM showed 3.12×106mm3 RBCs, 67.75 PLT, 8.92 g/100ml haemoglobin, 31.03% packed cell volume. This study shows that 45% PSM when replaced with FM had a beneficial impact on the growth, carcass composition and hematological parameters in L. rohita fingerlings.

Three experiments were performed to explore (i) the complete replacement of fish meal (FM) with a combination of fish residue meal (FRM, 65% round discarded fish + 35% byproduct), soy protein concentrate (SPC) from soymilk and corn gluten meal (CGM) in Trial 1 and (ii) the utilization of diets composed of increasing byproducts in FRM in the summer (Trial 2) and winter (Trial 3) seasons. In Trial 1, the ratio of (SPC + CGM):FM in the control diet (C) was 8:2. The FM component from diet C was replaced with FRM (diet, RM20), where the ratio of (SPC + CGM):FRM became 8:2, and this ratio was changed to 6:4, 4:6 and 2:8, and referred to as RM40, RM60 and RM80, respectively. In Trials 2 and 3, the ratios of round discarded fish and byproducts in FRM were adjusted to 65:35 (FRM1), 30:70 (FRM2) and 0:100 (FRM3), and the FRM component from diet RM40 in Trial 1 was replaced with FRM1, FRM2 and FRM3 to formulate diets RM1, RM2 and RM3, respectively. In Trials 1, 2 and 3, rearing periods were 10, 8 and 12 weeks, respectively. In Trials 1 and 3, there were no significant differences in growth parameters, nutrient retention efficiency or plasma constituents among the treatments, irrespective of the inclusion levels of FRM in the diets (p > 0.05). Although there were no significant differences in final mean weight (p > 0.05), daily feeding rate and feed conversion ratio in diet RM3 were significantly higher and lower, respectively, compared to the control group in Trial 2 (p < 0.05). These results suggest that FM can be entirely replaced with FRM, and that the total elimination of round discarded fish from FRM does not affect growth or health status in red sea bream either in summer or winter seasons.

This study analyzed the expression of growth‐related genes of olive flounder, Paralichthys olivaceus, fed a low‐fish meal (FM) diet to investigate the replacement of the FM diet in the field. The expression of growth hormone (GH)/insulin‐like growth factor (IGF) axis genes in the brain/pituitary/liver and plasma hormone concentrations were measured. A basal experimental diet was formulated using 63% FM and 12% soybean meal as the primary protein sources, and then two other diets were made by replacing 20% and 30% of the FM with soy protein concentrate, tankage meal, and poultry by‐products meal. Each diet was fed to duplicate groups of juvenile flounder (150 ± 3.0 g) twice a day. After 20 weeks, the plasma growth‐related hormone concentrations were similar between the control and FM20 groups. Moreover, GH/IGF axis gene mRNA expression in the brain/pituitary/liver was similar between the control and FM20 groups. Immunoblotting of muscle and liver showed the same pattern. This study showed the utility of replacing 20% of the FM diet and supports the possibility of field application.

The utilisation of insect meal-based fish feed as a substitute for conventional fish meal-based fish feed is considered as a promising innovative alternative to boost circularity in aquaculture and aquaponics. Basic research on its use in aquaponics is limited. So far, no reports on the effects of fish waste water, derived from a recirculating aquaculture system using Black Soldier Fly (BSF) meal-based diets, were available on the growth performance of lettuce. Therefore, this study aimed to compare the effect of reusing fish waste water from tilapia culture (as a base for the nutrient solution) fed with a fish meal-based diet (FM) and a BSF meal-based diet on resource use and lettuce growth in decoupled aquaponic systems. A conventional hydroponics nutrient solution (HP) served as control, and inorganic fertilisers were added to all nutrient solutions to reach comparable target concentrations. The experiment was conducted in a controlled climate chamber in nine separate hydroponics units, three per treatment. Lettuce fresh and dry weight, number of leaves, relative leaf chlorophyll concentration, water consumption, and the usage of inorganic fertilisers were measured. Micro- and macronutrients in the nutrient solutions were monitored in time series. Similar lettuce yield was seen in all treatments, with no significant effects on fresh and dry weight, the number of leaves, and relative chlorophyll values. Water use per plant was also similar between treatments, while the amount of total inorganic fertiliser required was 32% lower in FM and BSF compared to HP. Higher sodium concentrations were found in the FM nutrient solutions compared to BSF and HP. The results confirm that BSF-based diet is a promising alternative to FM-based diet in aquaponics with no negative effects on lettuce growth. Additionally, BSF-based diet might be beneficial in intensive, professional aquaponics applications due to the lower sodium concentration in the nutrient solution.

The aim of this study was to determine the apparent digestibility coefficients and digestible values of crude protein, ethereal extract, gross energy and dry matter of mulberry leaf meal (MLM) (Morus alba L.) as Nile tilapia (Oreochromis niloticus) feed. A total of 135 Nile tilapia juveniles were used, and the indirect methodology (Cr2O3) was applied for digestibility determinations. Mulberry leave meal presented good apparent digestible coefficients of protein, ethereal extract and energy with respective values of 0.94, 0.58 and 0.39. The mulberry leave meal thus comprises adequate digestible protein and digestible energy values, similar or better than other leafy foods, presenting potential for inclusion in Nile tilapia diets.

Recently, ecological and economic issues have affected fish meal (FM) supply, the main source of protein for shrimp. This triggered a search for alternative dietary protein sources for shrimp production. We studied the consequences of replacing FM with a defatted insect meal, ŸnMealTM (YM), comprised of yellow mealworm (Tenebrio molitor). Growth and immune parameters of juvenile Pacific white shrimp (Litopenaeus vannanmei) were compared after an eight-week feeding trial. Shrimp were kept in aquaria with densities of 60 and 40 shrimp/m2 and fed one of five diets in which a proportion of FM was replaced by YM. All diets were isoproteic, isoenergetic, and balanced in lysine and methionine. After the feeding trial, shrimp were challenged with pathogenic bacteria (Vibrio parahaemolyticus). Growth and feed conversion parameters improved when YM was included in shrimp diets; with the highest weight gain and best food conversion ratio (FCR) achieved when 50% of FM was replaced by YM versus the control diet that contained no YM (initial weight: 1.60 g/shrimp; growth: 5.27 vs. 3.94 g/shrimp; FCR 1.20 vs. 1.59). In challenged shrimp, mortality rates were significantly less among groups that received YM, with a 76.9% lower mortality rate in the 50% FM replacement group versus the control.

Aquaculture, the world’s fastest growing food sector, produces over half of all fish for human consumption. Aquaculture feeds include fishmeal and fish oil, extracted from wild-caught fish such as sardines, and poses ecological, food security, and economic drawbacks. Microalgae, yeasts, fungi, bacteria, and other alternative ingredients show promise as potential ingredients in aquafeeds that provide protein/amino acids, lipids, or omega-3 sources and sources of bioactive molecules. This review article discusses the issues that the literature often lacks data on, such as the recent development of using microorganisms, technological innovation, challenges, and opportunities to develop a low environmental footprint of aquaculture diet. The ingredients often require novel processing technology to improve digestibility and fish growth and reduce antinutritional factors. This is an important gap to fill because microalgae are the most frequently used organism in fish feed, particularly as a dietary supplement or mixed with other ingredients. The production, processing, and formulating steps can affect the nutritional qualities. Stepwise strategies are required to evaluate these ingredients for feed application, and in this article, I articulated the stepwise key approaches of evaluating nutritional and environmental response metrics to develop highly sustainable aquaculture feed using these microorganisms, which would guide a more judicious inclusion of these novel ingredients.

Protein hydrolysates have been used extensively as dietary protein for larval fish. Typically, they are expensive, difficult to produce, and show varying results when utilized for different species. This study proposed a practical hydrolysis method that utilizes endogenous enzymes within the body to “auto-hydrolyze”, or digest its tissue proteins with its own endogenous enzymes, and produce a fishmeal tailored to nutritional requirements and absorptive capacity of larval fish. The objectives for this experiment were to determine the: 1) effect of the proposed hydrolysis method on tissue protein breakdown level; and 2) effect of dietary inclusion of obtained hydrolysates on larval growth performance, using Zebrafish ( Danio rerio ). Whole-body adult Zebrafish were utilized to produce an unhydrolyzed fishmeal, and three fishmeals hydrolyzed for 1, 2, and 3 h, respectively. Three diets were formulated, defined by their dietary protein supply. The Unhydro diet contained unhydrolyzed Zebrafish meal. The 50% Hydro diet contained 50% Zebrafish meal hydrolysates and 50% unhydrolyzed Zebrafish meal. The 100% Hydro diet contained 100% Zebrafish meal hydrolysates. Five groups were utilized in this study, with three groups receiving one of the produced Zebrafish meal-based diets. Larvae fed a commercial starter diet and Artemia , respectively, were included as reference groups. Larval fish were randomly stocked into tanks (100 fish per tank) at 3 days-post-hatch (dph), and the trial was carried out until 18 dph. Electrophoretic analysis showed that the proposed hydrolysis method was able to efficiently hydrolyze the protein within Zebrafish body. The feeding trial results found no significant differences in weight, total length, or survival between the Unhydro, 50% Hydro, and 100% Hydro groups. The proposed hydrolysis method provides a practical and potentially cost-effective approach to producing species-specific fishmeal hydrolysates. Further research is necessary to determine whether the produced hydrolysates can improve the growth of larval fish in other species.

Background Sustainable aquaculture relies on multiple factors, including water quality, fish diets, and farmed fish. Replacing fishmeal (FM) with alternative protein sources is key for improving sustainability in aquaculture and promoting fish health. Indeed, great research efforts have been made to evaluate novel feed formulations, focusing especially on the effects on the fish gut microbiome. Few studies have explored host-environment interactions. In the present study, we evaluated the influence of novel insect-based ( Tenebrio molitor ) fish diets on the microbiome at the water-fish interface in an engineered rainbow trout ( Oncorhynchus mykiss ) farming ecosystem. Using 16S rRNA gene metabarcoding, we comprehensively analyzed the microbiomes of water, tank biofilm, fish intestinal mucus, fish cutis, and feed samples. Results Core microbiome analysis revealed the presence of a highly reduced core shared by all sample sources, constituted by Aeromonas spp., in both the control and novel feed test groups. Network analysis showed that samples were clustered based on the sample source, with no significant differences related to the feed formulation tested. Thus, the different diets did not seem to affect the environment (water and tank biofilm) and fish (cutis and intestinal mucus) microbiomes. To disentangle the contribution of feed at a finer scale, we performed a differential abundance analysis and observed differential enrichment/impoverishment in specific taxa, comparing the samples belonging to the control diet group and the insect-based diet group. Conclusions Omic exploration of the water-fish interface exposes patterns that are otherwise undetected. These data demonstrate a link between the environment and fish and show that subtle but significant differences are caused by feed composition. Thus, the research presented here is a step towards positively influencing the aquaculture environment and its microbiome.