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Obtaining crop above-ground biomass (AGB) information quickly and accurately is beneficial to farmland production management and the optimization of planting patterns. Many studies have confirmed that, due to canopy spectral saturation, AGB is underestimated in the multi-growth period of crops when using only optical vegetation indices. To solve this problem, this study obtains textures and crop height directly from ultrahigh-ground-resolution (GDS) red-green-blue (RGB) images to estimate the potato AGB in three key growth periods. Textures include a grayscale co-occurrence matrix texture (GLCM) and a Gabor wavelet texture. GLCM-based textures were extracted from seven-GDS (1, 5, 10, 30, 40, 50, and 60 cm) RGB images. Gabor-based textures were obtained from magnitude images on five scales (scales 1–5, labeled S1–S5, respectively). Potato crop height was extracted based on the generated crop height model. Finally, to estimate potato AGB, we used (i) GLCM-based textures from different GDS and their combinations, (ii) Gabor-based textures from different scales and their combinations, (iii) all GLCM-based textures combined with crop height, (iv) all Gabor-based textures combined with crop height, and (v) two types of textures combined with crop height by least-squares support vector machine (LSSVM), extreme learning machine, and partial least squares regression techniques. The results show that (i) potato crop height and AGB first increase and then decrease over the growth period; (ii) GDS and scales mainly affect the correlation between GLCM- and Gabor-based textures and AGB; (iii) to estimate AGB, GLCM-based textures of GDS1 and GDS30 work best when the GDS is between 1 and 5 cm and 10 and 60 cm, respectively (however, estimating potato AGB based on Gabor-based textures gradually deteriorates as the Gabor convolution kernel scale increases); (iv) the AGB estimation based on a single-type texture is not as good as estimates based on multi-resolution GLCM-based and multiscale Gabor-based textures (with the latter being the best); (v) different forms of textures combined with crop height using the LSSVM technique improved by 22.97, 14.63, 9.74, and 8.18% (normalized root mean square error) compared with using only all GLCM-based textures, all Gabor-based textures, the former combined with crop height, and the latter combined with crop height, respectively. Therefore, different forms of texture features obtained from RGB images acquired from unmanned aerial vehicles and combined with crop height improve the accuracy of potato AGB estimates under high coverage.

Predicting the crop nitrogen (N) nutrition status is critical for optimizing nitrogen fertilizer application. The present study examined the ability of multiple image features derived from unmanned aerial vehicle (UAV) RGB images for winter wheat N status estimation across multiple critical growth stages. The image features consisted of RGB-based vegetation indices (VIs), color parameters, and textures, which represented image features of different aspects and different types. To determine which N status indicators could be well-estimated, we considered two mass-based N status indicators (i.e., the leaf N concentration (LNC) and plant N concentration (PNC)) and two area-based N status indicators (i.e., the leaf N density (LND) and plant N density (PND)). Sixteen RGB-based VIs associated with crop growth were selected. Five color space models, including RGB, HSV, L*a*b*, L*c*h*, and L*u*v*, were used to quantify the winter wheat canopy color. The combination of Gaussian processes regression (GPR) and Gabor-based textures with four orientations and five scales was proposed to estimate the winter wheat N status. The gray level co-occurrence matrix (GLCM)-based textures with four orientations were extracted for comparison. The heterogeneity in the textures of different orientations was evaluated using the measures of mean and coefficient of variation (CV). The variable importance in projection (VIP) derived from partial least square regression (PLSR) and a band analysis tool based on Gaussian processes regression (GPR-BAT) were used to identify the best performing image features for the N status estimation. The results indicated that (1) the combination of RGB-based VIs or color parameters only could produce reliable estimates of PND and the GPR model based on the combination of color parameters yielded a higher accuracy for the estimation of PND (R2val = 0.571, RMSEval = 2.846 g/m2, and RPDval = 1.532), compared to that based on the combination of RGB-based VIs; (2) there was no significant heterogeneity in the textures of different orientations and the textures of 45 degrees were recommended in the winter wheat N status estimation; (3) compared with the RGB-based VIs and color parameters, the GPR model based on the Gabor-based textures produced a higher accuracy for the estimation of PND (R2val = 0.675, RMSEval = 2.493 g/m2, and RPDval = 1.748) and the PLSR model based on the GLCM-based textures produced a higher accuracy for the estimation of PNC (R2val = 0.612, RMSEval = 0.380%, and RPDval = 1.601); and (4) the combined use of RGB-based VIs, color parameters, and textures produced comparable estimation results to using textures alone. Both VIP-PLSR and GPR-BAT analyses confirmed that image textures contributed most to the estimation of winter wheat N status. The experimental results reveal the potential of image textures derived from high-definition UAV-based RGB images for the estimation of the winter wheat N status. They also suggest that a conventional low-cost digital camera mounted on a UAV could be well-suited for winter wheat N status monitoring in a fast and non-destructive way.