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"Germplasm resources"
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SSR markers development and their application in genetic diversity of burdock (Arctium lappa L.) germplasm
Background
Arctium lappa
L. is a medicinal edible homologous plant, commonly known as burdock or bardana, which belongs to the Asteraceae family and is abundant all over the world. Genetic diversity assessment is essential for
A. lappa
germplasm resource conservation and breeding. The assessment techniques include morphological, biochemical, and DNA marker analysis. However, the limited number of available DNA markers is insufficient to conduct related genetic diversity assessment studies.
Results
In this study, we conducted RNA sequencing of the
A. lappa
cultivar 'Yanagikawa Ideal' and developed SSR markers to characterize the genetic diversity and population structure of 56
A. lappa
accessions and 8 wild relative accessions. A total of 4,851 simple sequence repeats (SSRs) loci were identified. The proportions of mono-, di- and tri-nucleotide repeat motifs were 30.40%, 21.50% and 33.10%, respectively. We developed and verified the reliability of 28 SSR core primer pairs through electronic polymerase chain reaction (ePCR) and the PCR amplification process. The polymorphism information content (PIC) values of the 28 SSR core primer pairs ranged from 0.246 to 0.848, with 14 pairs of SSR primers displaying high polymorphism (PIC > 0.5). The 28 SSR core primer pairs showed 100% mobility in
Arctium tomentosum
Miller and 96.43% mobility in
Synurus deltoides
(Aiton) Nakai, indicating their high versatility. The average Shannon information index (I) was 1.231, and the average observed heterozygosity (Ho) was 0.132, the average expected heterozygosity (He) was 0.564. The 64 accessions were divided into three clusters at a genetic distance of 0.558. AMOVA analysis shows 83% genetic variation within populations and 17% among populations, highlighting implications for conservation and breeding strategies.
Conclusion
Our study provides 28 newly high-quality SSR markers to enhance genetic resource conservation and breeding programs for
A. lappa
, as well as to support comparative genomics and cross-species breeding strategies for related species.
Journal Article
Assessing the potential of genetic resource introduction into elite germplasm: a collaborative multiparental population for flint maize
Abstract Key message Implementing a collaborative pre-breeding multi-parental population efficiently identifies promising donor x elite pairs to enrich the flint maize elite germplasm. Abstract Genetic diversity is crucial for maintaining genetic gains and ensuring breeding programs’ long-term success. In a closed breeding program, selection inevitably leads to a loss of genetic diversity. While managing diversity can delay this loss, introducing external sources of diversity is necessary to bring back favorable genetic variation. Genetic resources exhibit greater diversity than elite materials, but their lower performance levels hinder their use. This is the case for European flint maize, for which elite germplasm has incorporated only a limited portion of the diversity available in landraces. To enrich the diversity of this elite genetic pool, we established an original cooperative maize bridging population that involves crosses between private elite materials and diversity donors to create improved genotypes that will facilitate the incorporation of original favorable variations. Twenty donor × elite BC1S2 families were created and phenotyped for hybrid value for yield related traits. Crosses showed contrasted means and variances and therefore contrasted potential in terms of selection as measured by their usefulness criterion (UC). Average expected mean performance gain over the initial elite material was 5%. The most promising donor for each elite line was identified. Results also suggest that one more generation, i.e., 3 in total, of crossing to the elite is required to fully exploit the potential of a donor. Altogether, our results support the usefulness of incorporating genetic resources into elite flint maize. They call for further effort to create fixed diversity donors and identify those most suitable for each elite program.
Journal Article
Heirloom seeds and their keepers : marginality and memory in the conservation of biological diversity
Farmers and gardeners have long appreciated a wide variety of plants and have nurtured them for meals, medicine, and exchange. But diversity too often has been surrendered to monocultures of fields and spirits, predisposing much of modern agriculture to uniformity and, consequently, vulnerability. Today it is primarily at the individual level - such as growing and saving a strange old bean variety or a curious-looking gourd - that any lasting conservation actually takes place. Heirloom Seeds and Their Keepers offers a much-needed, scientifically researched perspective on the contribution of seedsaving that illustrates its critical significance to the preservation of both cultural knowledge and crop diversity around the world. It opens new conversations between anthropology and biology, and between researchers and practitioners, as it honors conservation as a way of life.
Book
Valuing crop biodiversity: on-farm genetic resources and economic change
This book examines the challenges faced by farmers trying to maintain crop biodiversity in developing and transitional economies. Using a collection of empirical case studies of farmers and crop scientists across a range of agricultural economies and income levels, it presents economic tools and methods for valuing and managing crop biodiversity. It discusses the economic benefits of crop biodiversity for farmers and suggests ways in which crop biodiversity can be supported by national policies. The book provides an indispensable 'tool kit' for all those concerned with the development of strategies to facilitate sustainable management and conservation of crop genetic diversity for future generations.
eBook
Genetic resources, justice, and reconciliation : Canada and global access and benefit sharing
\"When the oral history of a medicinal plant as a genetic resource is used to develop a blockbuster drug, how is the contribution of indigenous peoples recognized in research and commercialization? What other ethical, legal, and policy issues come into play? Is it accurate for countries to self-identify as users or providers of genetic resources? This edited collection, which focuses on Canada, is the result of research conducted in partnership with indigenous peoples in that country, where melting permafrost and new sea lanes have opened the region's biodiversity, underscoring Canada's status as a user and provider of genetic resources and associated indigenous knowledge\"-- Provided by publisher.
Book
The genetic diversity of cacao and its utilization
The cacao (Theobroma cacao) plant is an important Neo-Tropical species whose natural habitat is the Amazon basin. Over the last 30 years there has been a considerable geographical expansion in the availability of cacao genetic resources. As a result the plant has a rich genetic diversity that exists at two levels: that of the primitive populations in the area of original distribution of the species, and that of the derived cultivated populations. This book provides a comprehensive review of our current knowledge of the diversity of the species. It starts by examining the diversity and inheritance of the characteristics of primitive populations in the Amazonian and Caribbean regions. It then looks at the evolution of diversity within cultivated populations first in South America and around the Caribbean, and then beyond the Americas. The book describes the inter-relationships between populations based on morphological and molecular markers. It also examines the conservation of genetic resources and how these genetic resources can be utilized to produce new cultivars.
eBook
Genetic resources and traditional knowledge : case studies and conflicting interests
This study describes efforts to define and protect traditional knowledge and the associated issues of access to genetic resources, from the negotiation of the Convention on Biological Diveristy through to the Declaration of Rights of Indigenous Peoples and the Nagoya Protocol.
Book
Farmers' bounty
Biological diversity is as crucial in agriculture as it is in nature, and it is equally important to the economic health of both industrial and nonindustrial societies. This book offers a sweeping assessment of crop diversity and the potential for its preservation. Stephen B. Brush develops a framework for investigating biological diversity in agriculture that focuses on the knowledge and practice of farmers, and he shows how this human ecology perspective can be applied to three global issues that affect crop resources.Brush defines the dimensions of crop diversity and outlines the essential questions surrounding it. He describes the techniques used to maintain diversity in major crops of three cradles of agriculture in which he has worked: potatoes in the Peruvian Andes, maize in Mexico, and wheat in Turkey. Finally, he explores the policy issues surrounding genetic erosion of crop varieties, conservation of crop diversity, and ownership of genetic resources.
eBook