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\"A pathbreaking investigation into homeostasis, the condition of that regulates human physiology within the range that makes possible not only the survival but also the flourishing of life. Antonio Damasio makes clear that we descend biologically, psychologically, and even socially from a long lineage that begins with single living cells; that our minds and cultures are linked by an invisible thread to the ways and means of ancient unicellular life and other primitive life-forms; and that inherent in our very chemistry is a powerful force, a striving toward life maintenance that governs life in all its guises, including the development of genes that help regulate and transmit life.\"-- Supplied by publisher.

The epithelial [Na.sup.+] channel (ENaC) emerged early in vertebrates and has played a role in [Na.sup.+] and fluid homeostasis throughout vertebrate evolution. We previously showed that proteolytic activation of the channel evolved at the water-to-land transition of vertebrates. Sensitivity to extracellular [Na.sup.+], known as [Na.sup.+] self-inhibition, reduces ENaC function when [Na.sup.+] concentrations are high and is a distinctive feature of the channel. A fourth ENaC subunit, [delta], emerged in jawed fishes from an [alpha] subunit gene duplication. Here, we analyzed 849 [alpha] and [delta] subunit sequences and found that a key Asp in a postulated [Na.sup.+] binding site was nearly always present in the [alpha] subunit, but frequently lost in the [delta] subunit (e.g. human). Analysis of site evolution and codon substitution rates provide evidence that the ancestral [alpha] subunit had the site and that purifying selection for the site relaxed in the [delta] subunit after its divergence from the [alpha] subunit, coinciding with a loss of [delta] subunit expression in renal tissues. We also show that the proposed [Na.sup.+] binding site in the [alpha] subunit is a bona fide site by conferring novel function to channels comprising human [delta] subunits. Together, our findings provide evidence that ENaC [Na.sup.+] self- inhibition improves fitness through its role in [Na.sup.+] homeostasis in vertebrates.

The Strange Order of Things is a pathbreaking investigation into homeostasis, the condition that regulates human physiology within the range that makes possible not only survival but also the flourishing of life. Antonio Damasio makes clear that we descend biologically, psychologically, and even socially from a long lineage that begins with single living cells; that our minds and cultures are linked by an invisible thread to the ways and means of ancient unicellular existence and other primitive life-forms; and that inherent in our very chemistry is a powerful force, a striving toward life maintenance that governs life in all its guises, including the development of genes that help regulate and transmit life. The Strange Order of Things is a landmark reflection that spans the biological and social sciences, offering a new way of understanding the origins of life, feeling, and culture.

[This corrects the article DOI: 10.1371/journal.pgen.1009233.].[This corrects the article DOI: 10.1371/journal.pgen.1009233.].

Under saline conditions, Suaeda salsa, as a typical halophyte, accumulates large amounts of Na.sup.+ in its leaves during optimal growth. Key transporters involved in Na.sup.+ accumulation in plants are HKT-type protein, the plasma membrane Na.sup.+/H.sup.+ transporter SOS1, and the tonoplast Na.sup.+/H.sup.+ antiporter NHX1. In this study, the function of SsHKT1;1 and its coordinate expression with SsSOS1 and SsNHX1 to regulate Na.sup.+ homeostasis in S. salsa was investigated. We showed, by yeast complementation assays, that SsHKT1;1 encoded a Na.sup.+-selective transporter, which located to the plasma membrane and was preferentially expressed within the stele, and was particularly abundant in xylem parenchyma and pericycle cells. When compared with a treatment of 25 mM NaCl, 150 mM NaCl greatly decreased the transcripts of SsHKT1;1, but maintained a relatively constant level of the expression of SsSOS1 in roots. Consequently, the synergistic effect of SsHKT1;1 and SsSOS1 would result in greater Na.sup.+ loading into the xylem under 150 mM NaCl than 25 mM NaCl. In leaves, 150 mM NaCl up-regulated the abundance of SsNHX1 compared with levels in 25 mM NaCl. This enabled the permanent sequestering of Na.sup.+ into leaf vacuoles. Overall, SsHKT1;1 functioned in reducing Na.sup.+ retrieval from the root xylem, and played an important role in coordinating with SsSOS1 and SsNHX1 to maintain Na.sup.+ accumulation in S. salsa under saline conditions.

Under saline conditions, Suaeda salsa, as a typical halophyte, accumulates large amounts of Na.sup.+ in its leaves during optimal growth. Key transporters involved in Na.sup.+ accumulation in plants are HKT-type protein, the plasma membrane Na.sup.+/H.sup.+ transporter SOS1, and the tonoplast Na.sup.+/H.sup.+ antiporter NHX1. In this study, the function of SsHKT1;1 and its coordinate expression with SsSOS1 and SsNHX1 to regulate Na.sup.+ homeostasis in S. salsa was investigated. We showed, by yeast complementation assays, that SsHKT1;1 encoded a Na.sup.+-selective transporter, which located to the plasma membrane and was preferentially expressed within the stele, and was particularly abundant in xylem parenchyma and pericycle cells. When compared with a treatment of 25 mM NaCl, 150 mM NaCl greatly decreased the transcripts of SsHKT1;1, but maintained a relatively constant level of the expression of SsSOS1 in roots. Consequently, the synergistic effect of SsHKT1;1 and SsSOS1 would result in greater Na.sup.+ loading into the xylem under 150 mM NaCl than 25 mM NaCl. In leaves, 150 mM NaCl up-regulated the abundance of SsNHX1 compared with levels in 25 mM NaCl. This enabled the permanent sequestering of Na.sup.+ into leaf vacuoles. Overall, SsHKT1;1 functioned in reducing Na.sup.+ retrieval from the root xylem, and played an important role in coordinating with SsSOS1 and SsNHX1 to maintain Na.sup.+ accumulation in S. salsa under saline conditions.

Abstract Background The gastrointestinal tract boasts the highest concentration of macrophages (MPs) in the body, acting as vital immune sentinels that oversee tissue balance. Three distinct subsets of MPs, namely CCR2+, Tim4+, and CCR2,Tim4 double-negative (DN) MPs, populate distinct gut niches. Their functional specialization suggests unique interactions within their non-hematopoietic tissue microenvironments. Despite their crucial roles, the intricacies of these interactions remain elusive. One recently discovered interaction involves a communication axis between MPs and neurons within the muscularis layer of the gut. Here, neurons secrete Colony Stimulating Factor 1 (CSF1), a survival factor for MPs. These MPs reciprocate by producing Bone Morphogenetic Protein 2 (BMP2), assisting neurons in regulating gut motility. Aims Based on the aforementioned interaction, we hypothesize that BMP2, derived from tissue-resident MPs, might influence functions of other tissue niches, subsequently prompting survival of MP via CSF1 production. Our aims are twofold: (1) Identify the role of BMP2 in gut macrophages; (2) Identify BMP2-responsive CSF1-producing tissue cells within the gut. Methods To discern the roles of MP subsets, we developed a conditional knockout mouse model, targeting Bmp2 expression in MPs. We validated successful Bmp2 deletion(BMP2 KO) through quantitative polymerase chain reaction (qPCR) and an in-situ hybridization immunofluorescence assay (RNAscope). Subsequently, single cell RNA (scRNAseq) was performed, combining samples from both control and KO small intestines. This was done to further confirm BMP2 KO and to identify BMP2-responsive CSF1-producing cells. Flow cytometry was then used to characterize the intestinal MPs in control and KO mice. Results Tim4+ MPs emerged as the primary source of BMP2. Comprehensive analysis using qPCR, RNAscope, and scRNAseq validated the complete KO of BMP2 across all macrophage subsets. The loss of MP-produced BMP2 led to a disruption in MP development. Notably, a marked reduction in DN and Tim4+ MPs was observed following deletion of Bmp2 in MPs, emphasizing the critical role of BMP2-driven CSF1 in sustaining these subsets. CSF1 producing cells co-expressing the cognate BMP receptor in the gut, were predominantly non-hematopoietic tissue cells. Among these, distinct fibroblast populations emerged as a potent but not the sole source of CSF1. Conclusions Our work underscores the involvement of multiple tissue cell populations, revealing an intricate network of interactions that extends beyond fibroblasts. This study unveils the multifaceted nature of tissue-MP interactions and their critical roles in maintaining gut homeostasis. Disturbances of these pathways may pave the way for disease by altering tissue homeostasis. Funding Agencies NSERC

In sport psychology, the use of biofeedback (BFB) is increasingly frequent, a noninvasive experimental procedure that allows the person to regulate their psychobiological functions and helps to become aware of internal processes that are not consciously controlled. Based on this, a new method was devised, PowerMens, which for the first time investigates these concepts integrated with specific training on visual attention. The subjects were 20 professional youth football players, divided into experimental and control groups. The research was conducted in pretest, training, and posttest, where the pre- and posttest consisted of a stress attention task. The experimental group conducted the BFB PowerMens training which integrates the BFB with Mental Games software promoting the control of the arousal level and the restoration of homeostasis. The aim of this research was to examine the psychophysiological reaction to the visual attention tasks that cause attentional and cognitive stress, predicting greater self-regulation and restoration of body homeostasis in the experimental group. The results are auspicious because they showed a better capacity for cognitive and emotional self-regulation, a restoration of homeostasis, and also an improvement in posttest time.