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"Heterotrophic bacteria."
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Investigating the physical and chemical factors affecting the microbial status of water in the water distribution network of Babol City with an emphasis on the HPC index
Safe and hygienic drinking water is crucial for public health. Secondary bacterial contamination, often caused by improper transport and distribution conditions, poses a significant threat. This study aimed to investigate the physical and chemical factors influencing the microbial status of drinking water in Babol's distribution network, focusing on the heterotrophic plate count (HPC) index.
Thirty-two water samples were collected randomly from Babol's rural areas and analyzed for HPC, residual free chlorine, turbidity, pH, and temperature. HPC was measured using the plate spreading technique on R2A agar. Descriptive statistics and regression analysis were employed to analyze the data.
Heterotrophic bacteria were detected in 61.4% of the samples, with 9.6% exceeding 500 CFU/ml. The average HPC was 140 CFU/ml, while residual free chlorine averaged 0.33 mg/l. In 21.4% of samples, residual chlorine was below 0.2 mg/l, and 19% had no detectable chlorine. Turbidity ranged from 0.05 to 3 NTU. Statistical analysis revealed significant positive correlations between HPC and pH, MPN, turbidity, and temperature (α < 0.05).
Regular HPC monitoring in Babol's drinking water distribution network is essential to identify contaminated areas and ensure adequate residual free chlorine levels (0.2-0.8 mg/l) for maintaining bacteriological water quality.
Journal Article
Isolation of Aerobic Heterotrophic Bacteria from a Microbial Mat with the Ability to Grow on and Remove Hexavalent Chromium through Biosorption and Bioreduction
Water pollution with toxic hexavalent chromium, Cr(VI), is an environmental threat that has a direct impact on living organisms. The use of microorganisms from microbial mats to remove Cr(VI) has scarcely been investigated. Here, we isolated aerobic heterotrophic bacteria from a Cr-polluted microbial mat found in a mining site in Oman, and investigated their ability to remove Cr(VI), and the underlying mechanism(s) of removal. All isolates fell phylogenetically into the genera
Enterobacter
,
Bacillus,
and
Cupriavidus
, and could completely remove 1 mg L
−1
Cr(VI) in 6 days. The strains could tolerate up to 2000 mg L
−1
Cr(VI), and exhibited the highest Cr(VI) removal rate at 100 ± 9 mg L
−1
d
−1
. Using scanning electron microscopy (SEM) coupled with elemental analysis, the strains were shown to adsorb Cr(VI) at their cell surfaces. The functional groups OH, NH
2
, Alkyl, Metal-O, and Cr(VI)-O were involved in the biosorption process. In addition, the strains were shown to reduce Cr(VI) to Cr(III) with the involvement of chromate reductase enzyme. We conclude that the aerobic heterotrophic bacteria isolated from Cr-polluted microbial mats use biosorption and bioreduction processes to remove Cr(VI) from wastewater.
Journal Article
Weekly variations of viruses and heterotrophic nanoflagellates and their potential impact on bacterioplankton in shallow waters of the central Red Sea
ABSTRACT
Bacterioplankton play a pivotal role in marine ecosystems. However, their temporal dynamics and underlying control mechanisms are poorly understood in tropical regions such as the Red Sea. Here, we assessed the impact of bottom-up (resource availability) and top-down (viruses and heterotrophic nanoflagellates) controls on bacterioplankton abundances by weekly sampling a coastal central Red Sea site in 2017. We monitored microbial abundances by flow cytometry together with a set of environmental variables including temperature, salinity, dissolved organic and inorganic nutrients and chlorophyll a. We distinguished five groups of heterotrophic bacteria depending on their physiological properties relative nucleic acid content, membrane integrity and cell-specific respiratory activity, two groups of Synechococcus cyanobacteria and three groups of viruses. Viruses controlled heterotrophic bacteria for most of the year, as supported by a negative correlation between their respective abundances and a positive one between bacterial mortality rates and mean viral abundances. On the contrary, heterotrophic nanoflagellates abundance covaried with that of heterotrophic bacteria. Heterotrophic nanoflagellates showed preference for larger bacteria from both the high and low nucleic acid content groups. Our results demonstrate that top-down control is fundamental in keeping heterotrophic bacterioplankton abundances low (< 5 × 10 5 cells mL−1) in Red Sea coastal waters.
The weekly variations of bacterioplankton standing stocks in the central Red Sea coastal water are apparently controlled by top-down regulators (viruses and heterotrophic nanoflagellates).
Journal Article
Prokaryotic community composition in a great shallow soda lake covered by large reed stands (Neusiedler See/Lake Fertő) as revealed by cultivation- and DNA-based analyses
Little is known about the detailed community composition of heterotrophic bacterioplankton in macrophyte-dominated littoral systems, where a considerable amount of dissolved organic carbon originates from aquatic macrophytes instead of phytoplankton. The aim of the present study was to reveal the effect of macrophytes on the microbial community and to elucidate their role in a macrophyte-dominated shallow soda lake, which can be characterised by a mosaic of open waters and reed marsh. Therefore, 16S rRNA gene amplicon sequencing, the most probable number method, cultivation of bacterial strains, EcoPlate and cultivation-based substrate utilisation techniques were applied. Differences in the structures of microbial communities were detected between the water and the sediment samples and between vegetated and unvegetated water samples. Planktonic bacterial communities of an inner pond and a reed-covered area showed significant similarities to each other. Woesearchaeia was the dominant archaeal taxon in the water samples, while Bathyarchaeia, ‘Marine Benthic Group D' and 'DHVEG-1’ were abundant in the sediment samples. The most probable number of heterotrophic bacteria was lower in the open water than in the reed-associated areas. The vast majority (83%) of the isolated bacterial strains from the water samples of the reed-covered area were able to grow on a medium containing reed extract as the sole source of carbon.
Journal Article
Marine macroalga-associated heterotroph Bacillus velezensis as prospective therapeutic agent
Marine macroalgae and their accompanying microbial flora were proved to be the reservoir of potential bioactive compounds with promising pharmacological applications. Heterotrophic bacteria concomitant with the marine algae were isolated and screened for their antibacterial potential against clinically recognized pathogens. The bacterial isolate with greater bioactive properties was identified as Bacillus velezensis MBTDLP1 (phylum Firmicutes), which was isolated from the marine macroalga Laurencia papillosa, by integrated morphological, biochemical and molecular characterization. B. velezensis showed promising antibacterial property against methicillin-resistant Staphylococcus aureus and Vibrio parahemolyticus with inhibition zone of 32–36 mm. Organic ethyl acetate extract of the isolate also displayed prospective antibacterial activity against the test pathogens (minimum inhibitory concentration 7.5–15 µg/mL), coupled with promising antioxidant (IC50 0.1–0.9 mg/mL against oxidants), anti-inflammatory (IC50 0.01 mg/mL against 5-lipoxygenase), and carbolytic enzyme attenuation properties (IC50 0.1–0.4 mg/mL in response to α-amylase and α-glucosidase). Significant anticancer potential against breast carcinoma (MCF-7) cells (IC50 0.03 mg/mL) coupled with lesser cytotoxicity to the normal fibroblast (3T3L) cells (IC50 0.14 mg/mL) were also recognized. The apoptosis assay could give reasonable outcome as the organic extract of B. velezensis induced apoptosis to 81% of the cancer cells while maintaining almost 60% viability in normal cells. The results put forward that B. velezensis MBTDLP1 could be used to isolate bioactive compounds with therapeutic potential and biomedical applications.
Journal Article
Differential visible spectral influence on carbon metabolism in heterotrophic marine flavobacteria
The visible spectrum of solar radiation is known to stimulate photoheterotrophic bacterial carbon metabolism. However, its impact on ‘strictly’ heterotrophic bacteria remains less explored. Here, we show that heterotrophic flavobacteria exhibit enhanced uptake and mineralization of dissolved organic carbon with increasing wavelengths of visible light, without employing any ‘known’ light-harvesting mechanisms. RNA sequencing identified blue light as a major constraint in the extracellular enzymatic hydrolysis of polymeric carbohydrates and acquisition of sugars, despite acting as a stimulus for inorganic carbon sequestration. In contrast, green–red and continuous full-spectrum lights activated diverse hydrolytic enzymes and sugar transporters, but obstructed inorganic carbon fixation. This ‘metabolic switching’ was apparent through limited nutrient uptake, suppressed light-sensitivity, oxidative stress response and promotion of inorganic carbon sequestration pathways under blue light. The visible light impact on metabolism may be of significant ecological relevance as it appears to promote cell-mediated mineralization of organic carbon in ‘green-colored’ chlorophyll-rich copiotrophic coastal seawater and inorganic carbon sequestration in ‘blue-colored’ oligotrophic open ocean. Thus, a novel regulatory role played by light on heterotrophic metabolism and a hidden potential of flavobacteria to sense and respond differentially to monochromatic lights influencing marine carbon cycling were unraveled.
Journal Article
Differential utilization patterns of dissolved organic phosphorus compounds by heterotrophic bacteria in two mountain lakes
Although phosphorus limitation is common in freshwaters and bacteria are known to use dissolved organic phosphorus (DOP), little is known about how efficiently DOP compounds are taken up by individual bacterial taxa. Here, we assessed bacterial uptake of three model DOP substrates in two mountain lakes and examined whether DOP uptake followed concentration-dependent patterns. We determined bulk uptake rates by the bacterioplankton and examined bacterial taxon-specific substrate uptake patterns using microautoradiography combined with catalyzed reporter deposition–fluorescence in situ hybridization. Our results show that in the oligotrophic alpine lake, bacteria took up ATP, glucose-6-phosphate and glycerol-3-phosphate to similar extents (mean 29.7 ± 4.3% Bacteria), whereas in the subalpine mesotrophic lake, ca. 40% of bacteria took up glucose-6-phosphate, but only ∼20% took up ATP or glycerol-3-phosphate. In both lakes, the R-BT cluster of Betaproteobacteria (lineage of genus Limnohabitans) was over-represented in glucose-6-phosphate and glycerol-3-phosphate uptake, whereas AcI Actinobacteria were under-represented in the uptake of those substrates. Alphaproteobacteria and Bacteroidetes contributed to DOP uptake proportionally to their in situ abundance. Our results demonstrate that R-BT Betaproteobacteria are the most active bacteria in DOP acquisition, whereas the abundant AcI Actinobacteria may either lack high affinity DOP uptake systems or have reduced phosphorus requirements.
In phosphorus-limited mountain lakes, the most abundant taxa, the AcI lineage of Actinobacteria and the R-BT cluster of Betaproteobacteria, exhibit strikingly different dissolved organic phosphorus uptake patterns.
Graphical Abstract Figure.
In phosphorus-limited mountain lakes, the most abundant taxa, the AcI lineage of Actinobacteria and the R-BT cluster of Betaproteobacteria, exhibit strikingly different dissolved organic phosphorus uptake patterns.
Journal Article
Water physicochemical quality as driver of spatial and temporal patterns of microbial community composition in lake ecosystems
This study aimed at monitoring and management of the surface water and potentially pathogenic microbes of Lake Tonga (Algeria) with respect to. It characterized the main bacterial diversity patterns of Lake Tonga and predicted from water physicochemical parameters and water quality index (WQI) the distribution of bacterial species and the main indicator groups of faecal water contamination. Water samples were taken monthly at three sampling sites of different water depths. Several physicochemical parameters were measured; of which some were included in computing WQI to characterize the water quality of the lake. Counting, isolation and bacterial identification methods were used to characterize the existing aerobic heterotrophic bacteria. The composition of the microbial community of the waterbody of Lake Tonga included an abundant culturable bacterial flora belonging to several bacterial families and whose specific richness varied between water depths of the sites sampled. Species richness of the bacteria identified phenotypically varied between 7 and 11 per sample. The site with shallow water was the richest in bacterial species, compared to moderate and deep waters. The redundancy analysis showed the main physicochemical drivers of the microbial community composition. Our findings showed that high WQI scores indicated the water quality deterioration which triggered the increase in total load of faecal indicator bacterial groups. This study identified in Lake Tonga an important culturable aerobic bacterial flora whose specific richness and distribution varied spatially following the effects of water physicochemical parameters. Lake Tonga needs an integrated management plan to mitigate human disturbances declining water quality.
Journal Article
Antibiotic-active heterotrophic Firmicutes sheltered in seaweeds: can they add new dimensions to future antimicrobial agents?
Appearance of drug-resistant microorganisms prompted researchers to unravel new environments for development of novel antimicrobial agents. Culture-supported analysis of heterotrophic bacteria associated with seaweeds yielded 152 strains, in that larger share of the isolates was embodied by Bacillus atrophaeus SHB2097 (54%), B. velezensis SHB2098 (24%), B. subtilis SHB2099 (12%), and B. amyloliquefaciens SHB20910 (10%). One of the most active strains characterized as B. atrophaeus SHB2097 (MW821482) with an inhibition zone more than 30 mm on spot-over-lawn experiment, was isolated from a seaweed Sargassum wightii, was selected for bioprospecting studies. Significant antibacterial potential was displayed by bacterial organic extract against vancomycin-resistant Enterococcus faecalis, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and Klebsiella pneumonia with minimum inhibitory concentration 6.25 µg/mL and comparable to the antibiotics ampicillin and chloramphenicol. The genes of type 1 pks (MZ222383, 700 bp) and hybrid nrps/pks (MZ222389, 1000–1400 bp) of B. atrophaeus MW821482 could be amplified. The bacterium displayed susceptibility to the commercially available antibiotic agents, and was negative for the pore-forming non-hemolytic hemolysin BL (hbl) and enterotoxin (nhe) genes, and therefore, was not pathogenic. The bacterium was found to possess genes (1000–1400 bp) involved in the biosynthesis of siderophore-class of compounds (MZ222387 and MZ222388) that showed 99% of similarity in BLAST search, and showed production of siderophore. Noteworthy antibacterial activities against clinically important pathogenic bacteria in conjunction with occurrence of genes coding for antimicrobial metabolites inferred that the marine heterotrophic bacterium B. atrophaeus SHB2097 could be used for the development of antibacterial agents against the emerging antibiotic resistance.
Journal Article