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Tissue microarray (TMA) technology is a high-throughput research tool, which has greatly facilitated and accelerated tissue analyses by in-situ technologies. TMAs are amenable to every research method that can be applied on the standard whole sections at enhanced speed. It plays a central role in target verification of results from cDNA arrays, expression profiling of tumors and tissues, and is proving to be a powerful platform for proteomic research. In this review article, primarily meant for students of pathology and oncology, we briefly discuss its basic methodology, applications and merits and limitations.

Tissue microarrays are a high-throughput method for the investigation of biomarkers in multiple tissue specimens at once. This technique allows for the analysis of up to 500 tissue samples in a single experiment using immunohistochemistry and in situ hybridization. Recently, cell lines and xenografts have been reduced to a tissue microarray format and are being applied to preclinical drug development. In clinical research, tissue microarrays are applied at multiple levels: comprehensive analysis of samples in the context of a clinical trial or across a population. Tissue microarrays play a central role in translational research, facilitating the discovery of molecules that have potential roles in the diagnosis, prognosis and prediction of response to therapy.

Tissue microarrays provide a high-throughput method for assessing a large number of samples by incorporating small cores of tissue into an array that can fit onto one microscope slide. Analyses of tissue microarrays were previously limited by semiquantitative protein expression analysis using brown stain (chromagen-based) methods. These methods are imperfect for protein expression analyses because of a smaller dynamic range and decreased ability for multiplexing many markers, as compared with objective quantitation of protein expression in tumor samples with fluorescence microscopy by a new technology called Automated Quantitative Analysis (AQUA™). By using AQUA analysis, tissue microarrays can serve a unique role as both a discovery tool and as a validation tool for nucleic-acid expression profiling-based target discoveries with results equivalent to enzyme-linked immunosorbent assay quantitation. The identification of novel prognostic markers can identify subsets of patients at high or low risk upon diagnosis, as well as new targets for potential future therapeutic development or metastatic disease treatment decisions. Thus, AQUA provides an unparalleled opportunity to advance personalized medicine through its ability to multiplex, quantitate and localize protein expression.