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New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range of consumer items, are of significant interest. Hence, this research emphasizes the biological approach of Ag-NPs through Egyptian henna leaves ( Lawsonia inermis Linn.) extracts and analysis of the prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses of prepared Ag-NPs were carried out through UV–visible (UV–Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and Fourier transform infrared (FTIR) analysis. UV–Vis reveals that Ag-NPs have a maximum peak at 460 nm in visible light. Structural characterization recorded peaks that corresponded to Bragg’s diffractions for silver nano-crystal, with average crystallite sizes varying from 28 to 60 nm. Antibacterial activities of Ag-NPs were examined, and it is observed that all microorganisms are very sensitive to biologically synthesized Ag-NPs.

This study is on photocatalytic degradation of pharmaceutical residues of atenolol (ATL) and acetaminophen (ACT) present in secondary effluent under visible light irradiation stimulated by Ag doped ZnO (Ag-ZnO) photocatalyst. Lawsonia inermis leaf extract was used for reduction of Zinc sulphate to ZnO nanoparticles (NPs). Further, ZnO NPs were doped with Ag and characterized by XRD, FT-IR, SEM-EDX, surface area analyzer, UV-Vis, and photoluminescence spectrometry to analyze the structure, morphology, chemical composition, and optical property. FT-IR analysis revealed major functional groups such as OH, C=O, and SEM analysis depicted the polyhedron shape of the NPs with size range of 100 nm. Ag-ZnO NPs were used in the photocatalytic degradation of ATL and ACT, and its removal was evaluated by varying initial contaminant concentration, catalyst dosage, and initial pH. Findings indicate that Ag-ZnO NPs demonstrated relative narrow bandgap and efficient charge separation that resulted in enhanced photocatalytic activity under visible light illumination. The photocatalytic degradation of ATL and ACT fitted well with pseudo-first-order kinetic model. Further, it was found that under optimal conditions of 5 mg/L of contaminants, pH of 8.5, and catalyst dose of 1 g/L, degradation efficiency of 70.2% (ATL) and 90.8% (ACT) was achieved for a reaction time of 120 min. More than 60% reduction in TOC was observed for both contaminants and OH• pathway was found to be the major removal process. Ag-ZnO photocatalyst showed good recycling performance, and these findings indicate that it could be cost effectively employed for removing emerging contaminants under visible light radiation.

•We evaluated the efficacy of a topical henna preparation in contact dermatitis in prosthetic limb users.•There are no effective, available and cheap treatments for skin problems of these patients.•This study was a randomized, double-blind, placebo-controlled clinical trial.•The results showed that the contact dermatitis symptoms were significantly improved after the study in the henna group.•The topical formulation of henna might be considered as a complementary remedy for improving contact dermatitis in patients using lower limb prosthetics. Contact dermatitis is a common complication in prosthetic limb users. There are no effective, available and cheap treatments for skin problems of these patients. In traditional Iranian medicine, henna (Lawsonia inermis L.) is a plant that has anti-inflammatory, antimicrobial and skin-enhancing properties, all of which are beneficial for people with artificial limbs. The aim of this study was to assess the efficacy of a topical henna preparation in management of contact dermatitis in patients using lower limb prosthetics. The current randomized, double-blind, placebo-controlled clinical trial was conducted on ninety-five participants with lower extremity amputation using limb prosthetics, aged 12–70 years who complained of contact dermatitis. They were randomly assigned to receive either two weeks of topical henna preparation every night as the intervention group, or topical placebo as the control group. Participants were instructed to spread henna preparation on the surfaces of the amputated limb that were in contact with the prosthesis. Severity of contact dermatitis symptoms was set as the primary outcome measure. Edema, papules and erythema were evaluated by a physician using standard color atlas. Secondary outcomes included symptoms such as burning, itching, pain, thickness and skin sweating evaluated by a self-administered questionnaire. A significant improvement was observed in the symptoms of contact dermatitis including skin edema, itching, sweating, skin thinning and pain (p-value<0.05) in the henna group compared to the placebo group. Skin burning decreased more in the henna group compared to the placebo group, but this was not statistically significant (p-value = 0.052). Moreover, skin redness significantly increased in the henna group (p-value = 0.001). Topical formulation of henna might be a complementary choice for improving contact dermatitis in patients using lower limb prosthetics.

Background: Cervicitis is a prevalent gynecologic disease, which does not usually respond to conventional treatments. Long-term cervicitis can cause serious health problems such as inflammation, infertility, and cancer. Henna oil, an herbal product in Persian medicine, is recommended for uterine diseases like cervicitis. Objective: This study aims to evaluate the efficacy of Henna oil as a vaginal suppository in combination with an antibiotic regimen in the treatment of cervicitis. Materials and Methods: This randomized placebo-controlled trial, included 92 nonmenopausal women with cervicitis at the Baqaipur Clinic of Shahid Sadoughi hospital in Yazd and the Persian Medicine Health Center in Ardakan, Yazd, Iran. Participants were further divided into either the Henna oil vaginal suppository group or the placebo group (n = 46/each group). During the study, the antibiotic treatment was administered to both groups. Cervicitis symptoms were compared between the groups and within each group. Results: Of 92 included individuals, 41 in each group completed the study. Results: revealed that significant differences were observed in some outcomes, including vaginal discharge (p < 0.001), cervical ulcer size (p < 0.001), dyspareunia (p = 0.046), and postcoital bleeding (p < 0.001), indicating that the treatment was more effective in the henna group compared to the placebo group. Conclusion: Findings supported that the vaginal suppository of Henna oil in combination with antibiotic therapy could be effective in the improvement of clinical symptoms of cervicitis regardless of its pathology. Key words: Lawsonia inermis, Persian+traditional medicine, Uterine cervicitis, Infertility, Vaginal suppository, Clinical trial.

Objective Infertility is one of the common problems in today’s modern world, it is estimated that 50% of infertility problems are related to men. Infertility in men has different causes. Oxidative stress and testis inflammation play an important role in sperm damage and infertility in men. The aim of the present study was to investigate the effect of Lawsonia inermis (L. inermis) on the changes made on the testicular tissue and sperm following the administration of lipopolysaccharide (LPS). Methods In this project, 40 mice were divided into four equal groups: (1) control: Tween 1% gavage, (2) Tween 1% + LPS (1 mg/kg; IP) and 3 and 4) L. inermis extract (300 and 500 mg/kg) along with LPS. After 7 days of the experimental period, the animals were anesthetized, and immediately, general sperm analysis was performed. Serum testosterone level, total antioxidant capacity and testicular histological changes were also investigated. Results In examining the quality of sperms, it was observed that the number, motility and viability of sperms in the LPS group are significantly reduced compared to the sham group. Relative improvement in sperm parameters was observed in the LPS group treated with a dose of 500 mg/kg L. inermis extract. The percentage of inhibition of oxidants (DPPH) was significantly decreased in the LPS group compared to the sham group. L. inermis extract increased the level of DPPH in the LPS group. Serum testosterone level was significantly decreased in all LPS and treatment groups. Histological examination also showed abnormality in the germinal epithelium and a decrease in the number of sperm cells in the seminiferous tubules in the LPS group. In the group treated with 500 mg/kg L. inermis extract, the number of sperms increased, and the layers of germ cells became better organized. In other cell lines in the germinal epithelium and Leydig cells, no difference was observed between the groups. Conclusion In general, the results of this study showed that LPS decreases sperm quality and increases testicular tissue damage, and L. inermis extract probably by reducing oxidants improves the number, motility and viability of sperms and testicular tissue damage.

The current study aimed to use biological treatment using plant extracts; extracted from some locally available plants: Lawsonia inermis (Henna), and Myrtus communis (Yass), against pathogenic bacteria isolated from heavy water in hospitals that do not have treatment plants. The two studied plants were extracted using two different solvents (water and alcohol). Diagnosis of these extracted using Gas chromatography (GC-MS) showed that the alcoholic extraction led to a higher percentage of effective phenolic compounds than the aqueous extract. Sewage water was used to isolate bacteria and according to the morphological, and biochemical, confirmed by using the vitke II instrument; six types of pathogenic bacteria were identified ( Staphylococcus lentus, Staphylococcus xylosus Sphingomonas paucimobilis, Escherichia coli, klebsiella oxytoca, Serratia ficaria ). The two Alcoholic plant extracted showed excellent antibacterial activity against all pathogenic bacteria than the water extracted as revealed by the diameter of the inhibition zone.

Considering the increased rate of microbial resistance to antibiotics and chemical side effects of antibiotics and antiseptics used for the treatment of periodontal disease, there is a need for an alternative antimicrobial agent with fewer complications. Medicinal herbs have recently become popular as novel antimicrobial agents. This study aimed to assess the antibacterial effects of hydroalcoholic extracts of , and on . Hydroalcoholic extracts of the three medicinal plants were obtained by the maceration technique and was cultured. Antimicrobial efficacy of the three medicinal plants was compared with that of 0.2% chlorhexidine (CHX) according to the Clinical and Laboratory Standards Institute protocol using agar disc diffusion and broth microdilution techniques. All tests were repeated three times. Hydroalcoholic extracts of all three plants had antimicrobial activity against . The minimum inhibitory concentration (MIC) of , , and was 78.1, 156.2, and 1666 μg/mL with no significant difference between them. The MIC of CHX was 3.33 μg/mL, which was significantly higher than that of B. serrata extract. Given that further studies confirm other properties of these extracts and their safety in terms of cytotoxicity and mutagenicity, hydroalcoholic extracts of and may be used in mouthwashes or local delivery systems to affect periodontal biofilm.

From the centuries, L. (Henna) is utilized in traditional health care system as a medicinal and cosmetic agent. The present study was intended to assess antiradical, DNA protective and antiproliferative activity of water extract of L. leaves (W-LI). Antioxidant activity was estimated using various assays such as DPPH, ABTS, superoxide anion radical scavenging, FRAP, deoxyribose degradation and DNA protection assay. Growth inhibitory effects of W-LI were assessed using MTT assay against different cancer cell lines HeLa, MCF-7, A549, C6 and COLO-205. From the results of antioxidant assays, it was found that W-LI quenched DPPH and ABTS cation radicals with IC value of 352.77 µg/ml and 380.87 µg/ml respectively. It demonstrated hydroxyl radical scavenging potential of 59.75 % at highest test dose of 1000 µg/ml in deoxyribose degradation assay. The results of FRAP assay showed that W-LI also possesses significant reducing activity. Extract inhibited hydroxyl radical induced pBR322 plasmid DNA strand scission, thus conferring DNA protection. Growth inhibition of various cancer cell lines was achieved to the varying extent on treatment with W-LI. Further, it was observed that activity was quite promising against colon cancer COLO-205 cells (GI 121.03 µg/ml). HPLC profiling of W-LI revealed the presence of different polyphenolic compounds such as ellagic acid, catechin, quercetin, kaempferol etc. which might be contributing towards antioxidant and cytotoxic activity. The present study demonstrated that polyphenols rich W-LI extract from leaves of possesses ability to inhibit oxidative radicals and cancer cells proliferation.