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Climate change amplifies the intensity and occurrence of dry periods leading to drought stress in vegetation. For monitoring vegetation stresses, sun-induced chlorophyll fluorescence (SIF) observations are a potential game-changer, as the SIF emission is mechanistically coupled to photosynthetic activity. Yet, the benefit of SIF for drought stress monitoring is not yet understood. This paper analyses the impact of drought stress on canopy-scale SIF emission and surface reflectance over a lettuce and mustard stand with continuous field spectrometer measurements. Here, the SIF measurements are linked to the plant’s photosynthetic efficiency, whereas the surface reflectance can be used to monitor the canopy structure. The mustard canopy showed a reduction in the biochemical component of its SIF emission (the fluorescence emission efficiency at 760 nm—ϵ760) as a reaction to drought stress, whereas its structural component (the Fluorescence Correction Vegetation Index—FCVI) barely showed a reaction. The lettuce canopy showed both an increase in the variability of its surface reflectance at a sub-daily scale and a decrease in ϵ760 during a drought stress event. These reactions occurred simultaneously, suggesting that sun-induced chlorophyll fluorescence and reflectance-based indices sensitive to the canopy structure provide complementary information. The intensity of these reactions depend on both the soil water availability and the atmospheric water demand. This paper highlights the potential for SIF from the upcoming FLuorescence EXplorer (FLEX) satellite to provide a unique insight on the plant’s water status. At the same time, data on the canopy reflectance with a sub-daily temporal resolution are a promising additional stress indicator for certain species.

Experimental data collected from 40 grasslands on 6 continents show that nutrients and herbivores can serve as counteracting forces to control local plant diversity; nutrient addition reduces local diversity through light limitation, and herbivory rescues diversity at sites where it alleviates light limitation. Shedding light on grazing and biodiversity Human activity has affected grassland biodiversity through the addition of both nutrients and grazing. Theory predicts that these factors could balance each other because they have opposing effects on light limitation, and this international collaboration across 40 experimental sites on six continents — from the 41 Nutrient Network (NutNet) cooperative — puts the theory to the test. The results demonstrate a consistent counteracting effect, with nutrient addition and herbivores jointly controlling plant diversity via light: nutrients reduce ground-level light thereby reducing plant diversity, and herbivores increase plant diversity by reducing competition for light among plants. This work will contribute towards more accurate modelling of the effects of grazing practices and nitrogen deposition on biodiversity in the world's grasslands. In a second paper in this issue of Nature , Yann Hautier et al . studied the influence of eutrophication in the NutNet grassland sites and show that the use of fertilizers is not only a threat to grassland biodiversity but also to the stabilizing effect it has on ecosystem functioning. Human alterations to nutrient cycles 1 , 2 and herbivore communities 3 , 4 , 5 , 6 , 7 are affecting global biodiversity dramatically 2 . Ecological theory predicts these changes should be strongly counteractive: nutrient addition drives plant species loss through intensified competition for light, whereas herbivores prevent competitive exclusion by increasing ground-level light, particularly in productive systems 8 , 9 . Here we use experimental data spanning a globally relevant range of conditions to test the hypothesis that herbaceous plant species losses caused by eutrophication may be offset by increased light availability due to herbivory. This experiment, replicated in 40 grasslands on 6 continents, demonstrates that nutrients and herbivores can serve as counteracting forces to control local plant diversity through light limitation, independent of site productivity, soil nitrogen, herbivore type and climate. Nutrient addition consistently reduced local diversity through light limitation, and herbivory rescued diversity at sites where it alleviated light limitation. Thus, species loss from anthropogenic eutrophication can be ameliorated in grasslands where herbivory increases ground-level light.

Estuarine fronts have significant effects on estuarine circulation, water quality, and productivity. However, there are limited studies on the joint ecological effects of sediment and plume fronts caused by tidal mixing and a low-salinity plume in and off the Changjiang River Estuary (CRE). Based on observational data during the summers of 1988–2016, we analyzed the spatial correlations between environmental factors and chlorophyll-a (Chl-a) concentrations along the two frontal boundaries. The water mass that is shoreward of the sediment front was characterized by the highest nutrients and total suspended matter (TSM); extensive light limitation caused by TSM consequently led to the lowest Chl-a. The water mass between the sediment and plume fronts displayed the highest Chl-a, which benefited from increased light availability due to rapid deposition of TSM across the sediment front and higher nutrients contributed by the plume and coastal upwelling. Shelf water mass beyond the plume front showed the lowest TSM and nutrients; the distinct reduction of nutrient supply resulted in a relatively low Chl-a. The spatial pattern of summer phytoplankton biomass tended to be a result of the balance of light and nutrients constructed by the two fronts and coastal upwelling in and off the CRE.

This work aims at developing an adequate theoretical basis for comparing assimilation of the ancestral C₃ pathway with CO₂ concentrating mechanisms (CCM) that have evolved to reduce photorespiratory yield losses. We present a novel model for C₃, C₂, C₂ + C₄ and C₄ photosynthesis simulating assimilatory metabolism, energetics and metabolite traffic at the leaf level. It integrates a mechanistic description of light reactions to simulate ATP and NADPH production, and a variable engagement of cyclic electron flow. The analytical solutions are compact and thus suitable for larger scale simulations. Inputs were derived with a comprehensive gas-exchange experiment. We show trade-offs in the operation of C₄ that are in line with ecophysiological data. C₄ has the potential to increase assimilation over C₃ at high temperatures and light intensities, but this benefit is reversed under low temperatures and light. We apply the model to simulate the introduction of progressively complex levels of CCM into C₃ rice, which feeds > 3.5 billion people. Increasing assimilation will require considerable modifications such as expressing the NAD(P)H Dehydrogenase-like complex and upregulating cyclic electron flow, enlarging the bundle sheath, and expressing suitable transporters to allow adequate metabolite traffic. The simpler C₂ rice may be a desirable alternative.

• Boreal forests undergo a strong seasonal photosynthetic cycle; however, the underlying processes remain incompletely characterized. Here, we present a novel analysis of the seasonal diffusional and biochemical limits to photosynthesis (A net) relative to temperature and light limitations in high-latitude mature Pinus sylvestris, including a high-resolution analysis of the seasonality of mesophyll conductance (g m) and its effect on the estimation of carboxylation capacity ( V C max ). • We used a custom-built gas-exchange system coupled to a carbon isotope analyser to obtain continuous measurements for the estimation of the relevant shoot gas-exchange parameters and quantified the biochemical and diffusional controls alongside the environmental controls over Anet. • The seasonality of A net was strongly dependent on V C max and the diffusional limitations. Stomatal limitation was low in spring and autumn but increased to 31% in June. By contrast, mesophyll limitation was nearly constant (19%). We found that V C max limited A net in the spring, whereas daily temperatures and the gradual reduction of light availability limited A net in the autumn, despite relatively high V C max . • We describe for the first time the role of mesophyll conductance in connection with seasonal trends in net photosynthesis of P. sylvestris, revealing a strong coordination between g m and A net, but not between g m and stomatal conductance.

Phototrophic microorganisms are promising resources for green biotechnology. Compared to heterotrophic microorganisms, however, the cellular economy of phototrophic growth is still insufficiently understood. We provide a quantitative analysis of light-limited, light-saturated, and light-inhibited growth of the cyanobacterium Synechocystis sp. PCC 6803 using a reproducible cultivation setup. We report key physiological parameters, including growth rate, cell size, and photosynthetic activity over a wide range of light intensities. Intracellular proteins were quantified to monitor proteome allocation as a function of growth rate. Among other physiological acclimations, we identify an upregulation of the translational machinery and downregulation of light harvesting components with increasing light intensity and growth rate. The resulting growth laws are discussed in the context of a coarse-grained model of phototrophic growth and available data obtained by a comprehensive literature search. Our insights into quantitative aspects of cyanobacterial acclimations to different growth rates have implications to understand and optimize photosynthetic productivity.

Colimitation of primary production is increasingly recognized as a dominant process across aquatic and terrestrial ecosystems. In streams, both nutrient availability and light availability have been shown to independently limit primary production, but colimitation by both light and nutrients is rarely considered. We used a series of nutrient-diffusing substrates (NDS) bioassays deployed across a range of light availability conditions in a single-study stream over two summers to determine the light level at which the limiting factor for benthic periphyton accrual transitioned from light to nutrients. Stream periphyton accrual was nutrient-limited in high-light patches, and light-limited in low-light patches, with the transition from being predominantly light-limited to being predominantly nutrient-limited occurring when daily light fluxes exceeded 3.5 mol m⁻² day⁻¹. We quantified light at each NDS bioassay location and at 5 m intervals throughout our two adjacent 160 m study reaches—one in structurally complex old-growth riparian forest and one bordered by more uniform second-growth forest. Although both reaches were colimited overall, the resource (light or nutrients) dominating limitation differed between the two riparian forest age/structure conditions. In the old-growth section, about three quarters of the reach was predominantly nutrient-limited, whereas in the second-growth reach only about a quarter of the streambed was nutrient-limited. In this stream, colimitation of benthic periphyton accrual by light and nutrients at the reach scale was an emergent property of the ecosystem that manifested as a result of high heterogeneity in riparian forest structure.

An understanding of regulating factors and early warning of Ulva prolifera biomass increase may reduce harm or prevent bloom disasters in the Yellow Sea. We investigated the minimum nutrient concentration and light-limiting depth (Z lim) for the growth of floating U. prolifera thalli. Bioavailable dissolved nitrogen (BDN) concentrations in most parts of the study area were almost always higher than the minimum N concentrations required for the growth of floating thalli, indicating no N limitation for the growth of floating thalli. However, the minimum N concentration required for the development of germlings into thalli was higher than BDN in the majority of the area north of 35° N. This indicated that germlings floating out of Subei Shoal were unable to grow into thalli because of N limitation. The minimum P concentration required for germling development was higher than the total dissolved P north of 35° N. This suggested that P limitation occurred for germlings floating out of Subei Shoal. The Z lim for the floating thalli was <0.1 m in most parts of Subei Shoal, which explained why the rapid growth of floating thalli only occurred when they floated out from the Subei Shoal. A grid pattern with the phased multiple increase in biomass per day was designed to predict the possible accumulated multiple increase in biomass (AcMp) when U. prolifera drifted northward following different trajectories. The predicted AcMp values in 2017, 2010, and 2009 were close to the ratio of the coverage area from remote sensing data. Such a grid pattern facilitates quick decisions in disaster prevention and reduction.

• The concentrations of nonstructural carbohydrates (NSCs) in plant tissues are commonly used as an indicator of total plant carbon (C) supply; but some evidence suggests the possibility for high NSC concentrations during periods of C limitation. Despite this uncertainty, NSC dynamics have not been investigated experimentally under long-term C limitation. • We exposed saplings of 10 temperate tree species differing in shade tolerance to 6% of ambient sunlight for 3 yr to induce C limitation, and also defoliated one species, Carpinus betulus, in the third season. Growth and NSC concentrations were monitored to determine C allocation. • Shade strongly reduced growth, but after an initial two-fold decrease, NSC concentrations of shaded saplings recovered to the level of unshaded saplings by the third season. NSC concentrations were generally more depleted under shade after leaf flush, and following herbivore attacks. Only under shade did artificial defoliation lead to mortality and depleted NSC concentrations in surviving individuals. • We conclude that, irrespective of shade tolerance, C storage is maintained under prolonged shading, and thus high NSC concentrations can occur during C limitation. Yet, our results also suggest that decreased NSC concentrations are indicative of C limitation, and that additional leaf loss can lead to lethal C shortage in deep shade.

1. Potential variability in nutrient limitation among tree size classes, functional groups and species calls for an integrated community- and ecosystem-level perspective of lowland tropical rainforest nutrient limitation. In particular, canopy trees determine ecosystem nutrient conditions, but competitive success for nutrients and light during the sapling bottleneck determines canopy composition. 2. We conducted an in situ multi-nutrient sapling fertilization experiment at La Selva Biological Station, Costa Rica, to determine how functional group identity, species identity and light availability can impact nutrient limitation of stem growth in three functional groups and nine species. 3. Despite high soil fertility, we found nutrient-light limitation in two functional groups and four species. Unexpectedly, the nitrogen-fixing (\"N₂ fixers\") and shade-tolerant functional groups were significantly nutrient limited, while the light-demanding functional group was not. 4. This was partially explained by species-level variation in nutrient limitation within these functional groups, with only some species conforming to the prediction of stronger nutrient limitation in light demanders compared to shade-tolerants. 5. Most surprisingly, we found strong nutrient limitation at low-light levels in the N₂ fixers (which were shade-tolerant), but not in the shade-tolerant non-fixers. We hypothesize that the N₂ fixers were actually nitrogen limited at low-light levels because of their nitrogen-rich leaves and the high carbon cost of their symbionts. 6. This finding suggests a highly shade-tolerant, N₂ fixation strategy, in addition to the perception that N₂ fixation is mostly advantageous in high-light environments during early and gap succession. The shade-tolerant, N₂ fixation strategy may be part of a sapling and canopy tree feedback, where the canopy N₂ fixers enrich the soil N, enhancing growth of their shade-tolerant saplings relative to non-fixing competitors, enabling further canopy domination by shade-tolerant N₂ fixers, as seen at La Selva. 7. Synthesis. The pervasiveness of functional group- and species-specific nutrient and light co-limitation in our saplings indicates that these interactions likely play an important role in the dynamics of lowland tropical rainforest nutrient limitation, potentially via other such sapling and canopy tree feedbacks as the one hypothesized.