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Usage of chemicals such as malachite green to disinfect fish eggs in aquaculture has been banned in many countries around the world due to its environmental negative impact. This study aimed to evaluate the Zingiber officinale rhizomes extract as an antifungal agent during incubation period of Bunnei eggs and comparison of its effects with malachite green. For this purpose 24 hours after fertilization, methanolic extract of Zingiber officinale rhizomes in four groups with a concentration of 500, 750, 1000, and 1250 mg/L and three replications per concentration in a (10 minute) bath every 12 hours and malachite green group with a concentration of 0.1 mg/L for (6 minutes) bath was used twice a day and control group without antifungal agents under the same physiochemical conditions as other treatments were tested. The present study showed that the highest percentage of fungal infection was 41.82±0.5 % for the control group and the lowest 0.3±0.5 % was obtained for the treatment of 1250 mg/L of Zingiber officinale rhizomes. Also, the results showed that there were significant differences between treatments of different concentrates of  Zingiber officinale rhizomes  and  with malachite green group in fungal infection, percentage of fertilization and survival do not exist (P>0.05.)  No apparent deformity and abnormality were observed in the hatched larvae of Bunnei, so the extract of Zingiber officinale rhizomes with concentration 500 mg /L can be used to disinfect  Bunnei eggs during Incubation period and safe material for humans and the environment.  

This study was aimed to estimate the antioxidants, antibacterial, and antifungal activities of methanolic extract from soybean seeds against pathogenic microorganisms. The methanolic extract was prepared from defatted soybean flour. Total phenolics content (TPC), total flavonoid content (TFC), and the major phenolic, flavonoid, and isoflavone compounds in soybean extract were estimated by HPLC. On the other hand, biological activities such as antioxidants, antibacterial, and antifungal activities were evaluated. The value of phenolic compounds was 8.59 mg GAE/g extract, while the value of flavonoids was 0.82 mg QE/g extract. The chemicals listed below were chromatographed and identified: syringic acid, quercetin, gallic acid, benzoic acid, genistein, daidzein, p-coumaric acid, glycitein, and ferulic acid. The methanolic extract showed the antibacterial and antifungal activity against tested microorganisms. Considering the results, it is possible to employ the methanolic extract from soybean seeds, which is rich in phenolic chemicals, as an antioxidant, antibacterial, and antifungal agent. It functions well as a pure, natural product.

Globally, mosquito-borne diseases, particlulalry those transmitted by Culex quincquefasciatus pose a significant public health challenge. Traditional methods of eradication using synthetic insecticides pose environmental concerns and a risk of developing insecticide-resistant varieties. Here, the use of plant-based biopesticides offers a safer and sustainable alternative. The study aimed to investigate the insecticidal properties of Flemingia wightiana (FW) leaves by synthesising leaf extracts and silver nanoparticles. The toxicity of the test samples was tested on Oreochromis niloticus at concentrations of 0.1, 0.5, and 1 mg/L. Furthermore, the test samples were subjected to lethality assay on C. quinquefasciatus. Laboratory bioassays were conducted to evaluate the efficacy of crude extract and silver nanoparticles of F. wightiana at varying concentrations, specifically 0.5, 1, 2, and 4 mg/L. Ovicidal, emergency and larvicidal activity were studied. The results indicated significant larvicidal activity and exhibited better potential for toxicity against Culex larvae treated with AgNPs. FW-AgNPs have substantial effect in delaying the hatching of mosquito eggs. Moulting of larvae from one instar to the next was also delayed by treatment with AgNPs. The findings demonstrated that FW-AgNPs play a significant role in controlling C. quinquefasciatus populations.  

Objective: To estimate the hepatoprotective effects of the methanolic seed extract of Eugenia jambolana Lam. (Myrtaceae), in Wistar albino rats treated with carbon tetrachloride (CCl 4 ). Materials and Methods: Liver damage in rats treated with CCl 4 (1ml/kg/Bw, administered subcutaneously, on alternate days for one week) was studied by assessing parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), acid phosphatase (ACP) and bilirubin (total and direct). The effect of co-administration of Eugenia jambolana Lam. (doses 100, 200 and 400 mg/kg p. o.) on the above parameters was investigated. These biochemical observations were supplemented by weight and histological examination of liver sections. Liv.52 ® was used as positive control. Data were analyzed by one way anova, followed by Scheff′s/Dunnett′s test. Results: Administration of Eugenia jambolana Lam. (doses 100, 200 and 400 mg/kg p. o.) significantly prevented carbon tetrachloride induced elevation of serum SGOT, SGPT, ALP, ACP and bilirubin (total and direct) level. Histological examination of the liver section revealed hepatic regeneration, after administration of various doses of Eugenia jambolana Lam. The results were comparable to that of Liv.52 ®. Conclusion: The study suggests preventive action of Eugenia jambolana Lam. in carbon tetrachloride induced liver toxicity. Hepatic cell regeneration process was dose dependent.

Objective: To study analgesic and anti-inflammatory activities of a methanolic extract (ME) of Argyreia speciosa (AS) root powder. Materials and Methods: The study was carried out using male albino mice (20-25 gm) and male wistar rats (100-150gm). The ME was prepared using soxhlet extraction process. The effect of ME of A. speciosa was investigated for analgesic activity using acetic acid-induced abdominal constriction, tail immersion method and hot plate method. The anti-inflammatory activity of ME of AS roots was studied using carrageenan-induced rat paw edema. Result: The ME of A. speciosa root was used in pain and inflammation models. The analgesic activity of AS at the dose of (30,100, and 300 mg/kg p.o) showed significant (P< 0.01) decrease in acetic acid-induced writhing, whereas ME of A. speciosa at the dose of (100, 300 mg/kg p.o) showed significant (P< 0.01) increase in latency to tail flick in tail immersion method and elevated mean basal reaction time in hot plate method. The ME of the A. speciosa at doses (30, 100, and 300mg/kg) showed significant (P < 0.01) inhibition of carrageenan induced hind paw edema in rats. Conclusion: The ME of A. speciosa showed significant analgesic and anti-inflammatory activity in mice and rat.

Currently, the potential utilization of fruits and vegetable waste as a source of micronutrients and antioxidants has increased. The present study, therefore, aimed to determine the antimicrobial and anti-inflammatory activities of Citrus nobilis peel extract. A modified solvent evaporation technique was employed for peel extract preparation. For effective utilization of the natural product, quantitative analysis of phenolic compounds was carried out using liquid chromatography and mass spectroscopy technique. Phenolic and flavonoids were present in high amounts, while β-carotene and lycopene were present in vestigial amounts. The antimicrobial efficiency of peel extract was evaluated against four bacterial strains including Staphylococcus aureus (MTCC 3160), Klebsiella pneumoniae (MTCC 3384), Pseudomonas aeruginosa (MTCC 2295), and Salmonella typhimurium (MTCC 1254), and one fungal strain Candida albicans (MTCC 183), and zone of inhibition was comparable to the positive control streptomycin and amphotericin B, respectively. The extract of Citrus nobilis peels showed effective anti-inflammatory activity during human red blood cell membrane stabilization (HRBC) and albumin denaturation assay. The extracts also exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity ranging from 53.46 to 81.13%. Therefore, the obtained results suggest that Citrus nobilis peel could be used as an excellent source of polyphenols and transformed into value-added products.

Quitral ( ), a Chilean and Argentine parasitic mistletoe, is traditionally used by Mapuche natives to treat stomach ulcers, nervous disorders, and cholesterol reduction, although scientific support is scarce. Methanolic and chloroform extracts from its leaves and stems were prepared. Chemical analysis included antioxidant capacity assays (ORAC-FL and DPPH) and chromatographic determinations. The antimicrobial activity was tested against nine bacteria and two yeast strains. Additionally, cytotoxicity (hemolysis) and toxicity (against ) assays were performed. The results revealed that the methanolic leaf extracts had the highest ORAC-FL value, with DPPH assays showing solvent-dependent differences. Thirty-one compounds were tentatively identified, of which 61% were phenolic compounds, primarily flavonoids like quercetin and its derivatives. Antimicrobial results showed activity against Gram-positive bacteria ( , , , and ), but not against yeast and . Methanolic extracts induced dose-dependent erythrocyte hemolysis, while chloroform extracts showed no relevant cytotoxicity. Toxicity against was also dose-dependent for methanolic extracts; leaf extract reduced survival at 50 mg mL after 24 h. These findings partially validate some traditional uses, highlight the importance of solvent polarity in extraction and biological effects, and establish quitral as a flavonoid source.

Currently, the potential utilization of fruits and vegetable waste as a source of micronutrients and antioxidants has increased. The present study, therefore, aimed to determine the antimicrobial and anti-inflammatory activities of peel extract. A modified solvent evaporation technique was employed for peel extract preparation. For effective utilization of the natural product, quantitative analysis of phenolic compounds was carried out using liquid chromatography and mass spectroscopy technique. Phenolic and flavonoids were present in high amounts, while β-carotene and lycopene were present in vestigial amounts. The antimicrobial efficiency of peel extract was evaluated against four bacterial strains including (MTCC 3160), (MTCC 3384), (MTCC 2295), and (MTCC 1254), and one fungal strain (MTCC 183), and zone of inhibition was comparable to the positive control streptomycin and amphotericin B, respectively. The extract of peels showed effective anti-inflammatory activity during human red blood cell membrane stabilization (HRBC) and albumin denaturation assay. The extracts also exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity ranging from 53.46 to 81.13%. Therefore, the obtained results suggest that peel could be used as an excellent source of polyphenols and transformed into value-added products.

Dracaena reflexa, a traditionally significant medicinal plant, has not been extensively explored before for its phytochemical and biological potential. The present study was conducted to evaluate the bioactive phytochemicals and in vitro biological activities of D. reflexa, and perform in silico molecular docking validation of D. reflexa. The bioactive phytochemicals were assessed by preliminary phytochemical testing, total bioactive contents, and GC-MS analysis. For biological evaluation, the antioxidant (DPPH, ABTS, CUPRAC, and ABTS), antibacterial, thrombolytic, and enzyme inhibition (tyrosinase and cholinesterase enzymes) potential were determined. The highest level of total phenolic contents (92.72 ± 0.79 mg GAE/g extract) was found in the n-butanol fraction while the maximum total flavonoid content (110 ± 0.83 mg QE/g extract) was observed in methanolic extract. The results showed that n-butanol fraction exhibited very significant tyrosinase inhibition activity (73.46 ± 0.80) and acetylcholinesterase inhibition activity (64.06 ± 2.65%) as compared to other fractions and comparable to the standard compounds (kojic acid and galantamine). The methanolic extract was considered to have moderate butyrylcholinesterase inhibition activity (50.97 ± 063) as compared to the standard compound galantamine (53.671 ± 0.97%). The GC-MS analysis of the n-hexane fraction resulted in the tentative identification of 120 bioactive phytochemicals. Furthermore, the major compounds as identified by GC-MS were analyzed using in silico molecular docking studies to determine the binding affinity between the ligands and the enzymes (tyrosinase, acetylcholinesterase, and butyrylcholinesterase enzymes). The results of this study suggest that Dracaena reflexa has unquestionable pharmaceutical importance and it should be further explored for the isolation of secondary metabolites that can be employed for the treatment of different diseases.