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Background Social tolerance strongly influences the patterns of affiliation and aggression in animal societies. However, not much is known about the variation of social tolerance in species living in dispersed social systems that combine solitary foraging activities with the need of coordinating social interactions with conspecifics on a regular basis. This study aims to investigate the sources of variation in social tolerance within a Malagasy primate radiation with dispersed social systems, the mouse lemurs ( Microcebus spp.). Six mouse lemur species were selected as model species that belong to three different taxonomic clades, live in two types of forest environments (dry and humid), and differed in this study with respect to their reproductive activity. Six male–female and six male–male dyads of each species were tested temporarily in a standardized social encounter paradigm in Madagascar to collect data on joint use of space, non-agonistic body contacts, aggression rates, the number of conflicts and the establishment of intra- and intersexual dominance. Results Male–female dyads of the six species differed significantly in the frequency of affiliative and agonistic behaviors. In contrast, the variations between male–male dyads could not be explained by one parameter only, but clade membership, forest type, reproductive state as well as species were all suggested to be partially influential. Only one species ( Microcebus mamiratra ) showed signals of unambiguous female dominance in all male–female dyads, whereas the others had no or only a few dyads with female dominance. Conclusions Variations in social tolerance and its consequences are most likely influenced by two factors, ecology (via forest type) and physiology (via reproductive activity), and only to a lesser extent by clade membership. The study suggests that mouse lemur females have higher aggression rates and more agonistic conflicts with males when females in the population are reproducing, at least in resource-rich humid forests. The study confirms a high degree of social plasticity between species in these small solitary foragers that supports their taxonomic distinctiveness and requires further scientific attention.

Understanding the mechanisms by which similar species coexist in sympatry is a major driver of ecological research. Niche partitioning and ecological plasticity can facilitate spatial and habitat use overlap between generalist and specialist species. Mouse lemurs (Microcebus spp.) are a highly speciose group of small primates that are endemic to the forests of Madagascar. In northwestern Madagascar, the relatively widespread M. murinus occurs sympatrically with the microendemic M. ravelobensis. We investigated spatial distributions and densities of these two species across a mangrove–dry forest habitat gradient in Mariarano commune. We used capture-mark-recapture techniques and nocturnal line transect surveys along six transects during June and July 2017. Spatial capture-recapture and distance sampling models were used to estimate lemur densities across habitat types. The congeners displayed differential patterns of spatial distribution and densities. Microcebus murinus was found in similar densities across all habitat types, while M. ravelobensis was found at much higher densities in dry forests compared with mangroves. This suggests that the generalist M. murinus uses a wider array of habitats more evenly than the specialist M. ravelobensis. Our study provides empirical evidence of how cryptic lemur species differ in their habitat use and distribution across an environmental gradient and provides new insights into their ecology in an understudied habitat. Lemurs are one of the most threatened groups of mammals in the world, and understanding how these species are distributed across different forest types is crucial for planning and implementing conservation measures to protect lemur habitat.

Species can either maintain a certain social organization in different habitats or show different social organizations in similar habitats. The reasons underlying this variability are not always clear but might have consequences for population dynamics, especially under changing environmental conditions. Among mammals, the primate genus Microcebus lives in small groups of closely related females, derived from female philopatry and dispersed males, as illustrated by the well-studied Microcebus murinus. Here, we studied the genetic structure of a population of the congeneric Microcebus griseorufus, inhabiting three adjacent habitats with different resource availabilities. In order to learn more about the plasticity of the species’ social organization under these different conditions, we analyzed the spatial arrangement of mitochondrial haplotypes of 122 individuals. The study revealed high haplotype diversity and a pronounced difference in spatial distribution between the sexes. Females exhibited spatial aggregation of haplotypes, suggesting a system of female philopatry and matrilines, similar to M. murinus. Male haplotypes were dispersed, and males were more likely to carry rare haplotypes, indicating higher dispersal activity. These findings hint towards the unity of the social organization across the genus Microcebus, suggesting a phylogenetic origin of the social organization. Yet, with decreasing resources, the clustering of female haplotypes declined and approached a random distribution in the marginal habitat, with cluster sizes correlating with resource availability as predicted by the socioecological model. Our study supports the notion that social organization is shaped by both phylogenetic origin and ecological conditions, at least in these small primates.Significance statementImpacts of habitat degradation are mostly described in terms of changes in population densities in relation to the reduction of resources. This neglects the possible effects of altered social organizations due to declining resources or population densities. Using a genetic sampling of three subpopulations of mouse lemurs in Madagascar along a gradient of food availability up to the limit of the species’ ecological tolerance, we show that their social organization consisting of spatial clusters of closely related females and overdispersed males converges towards random spatial distributions of both sexes with declining food availability.

Olfactory communication is highly important for nocturnal mammals, especially for solitary foragers, but knowledge is still limited for nocturnal primates. Mouse lemurs ( Microcebus spp.) are nocturnal solitary foragers with a dispersed lifestyle and frequently use chemo-sensory signalling behaviour for governing social interactions. Different mouse lemur species can co-occur in a given forest but it is unknown whether olfaction is involved in species recognition. We first screened 24 captive mouse lemurs (9  M. murinus , 15  M. lehilahytsara ) for their olfactory learning potential in an experimental arena and then tested the species discrimination ability with urine odour in an operant conditioning paradigm in four individuals. The majority of the screened animals (75%) did not pass the screening criteria within a 2-week test period. However, all four final test animals, two M. murinus and two M. lehilahytsara , were successfully trained in a 5-step-conditioning process to reliably discriminate conspecific from heterospecific urine odour (requiring an overall median of 293 trials). Findings complement previous studies on the role of acoustic signalling and suggest that olfaction may be an important additional mechanism for species discrimination.

Seasonal reproduction is widespread among primates but the degree of reproductive synchrony and plasticity can vary, even between closely related species. This study compares the dynamics of female reproductive seasonality in two mouse lemur species, Microcebus murinus and M. ravelobensis, in Ankarafantsika National Park, Madagascar, across 24 years. We collected 4321 records of female reproductive state from 1033 individual females (319 M. murinus, 714 M. ravelobensis). The analyses revealed disparate reproductive schedules: While female M. murinus showed high degrees of reproductive synchrony throughout all years, leading to the production of two successive litters, the seasonal onset of estrus (= reproductive activation) in female M. ravelobensis was more flexible than in M. murinus, starting 2-4 weeks earlier, varying by up to 4 weeks between years, and being less synchronized. M. ravelobensis females became reproductively active later in years with more rainfall, in particular rain in February, but the timing of reproductive activation was not related to differences in temperatures. The likelihood of early conception was significantly lower in M. ravelobensis than in M. murinus. This was partly due to delayed reproductive activation in young animals, and a lower likelihood of early conception for females with low body mass in M. ravelobensis. Our results suggest high, adaptive reproductive plasticity in M. ravelobensis that may enable individuals to respond flexibly to yearly environmental changes and expand the reproductive period under favorable conditions. These species differences in reproductive schedules may be the result of the divergent evolutionary histories of the two mouse lemur species in different parts of Madagascar.

Mouse lemurs Microcebus spp. are small, nocturnal primates endemic to Madagascar. The genus is extraordinarily diverse, with 25 extant species, several of which have been described recently. The Endangered Claire's mouse lemur Microcebus mamiratra was first described in 2006, but, similarly to other newly described mouse lemurs, remains understudied, and estimates of its population size are unavailable, hampering effective conservation management. We conducted line transect distance sampling surveys of M. mamiratra across several habitat types in and around Lokobe National Park on the island of Nosy Be in north-western Madagascar. Using a systematic random design we surveyed 15 transects over a 6-week period in 2023, recording 92 detections from a total survey effort of 46.5 km. We estimate the density of M. mamiratra on Nosy Be to be 125.1 individuals/km2, which extrapolates to an estimate of c. 4,700 individuals across the forested areas of its range on the island. Our results indicate that Nosy Be harbours moderately high densities of M. mamiratra, with the highest encounter rates in the unprotected secondary and degraded forests around Lokobe National Park. Our population estimate will inform future conservation status assessments and conservation planning for this range-restricted species and provide a baseline for monitoring population changes over time. We present recommendations for the conservation of M. mamiratra and highlight the potential for lemur watching, sustained by the strong tourism industry on Nosy Be, to help protect lemur habitat and generate economic opportunities for local communities.

Lentiviruses chronically infect a broad range of mammalian species and have been transmitted from primates to humans, giving rise to multiple outbreaks of HIV infection over the past century. Although the circumstances surrounding these recent zoonoses are becoming clearer, the nature and timescale of interaction between lentiviruses and primates remains unknown. Here, we report the discovery of an endogenous lentivirus in the genome of the gray mouse lemur (Microcebus murinus), a strepsirrhine primate from Madagascar, demonstrating that lentiviruses are capable of invading the primate germ line. Phylogenetic analysis places gray mouse lemur prosimian immunodeficiency virus (pSIVgml) basal to all known primate lentiviruses and, consistent with this, its genomic organization is intermediate between the nonprimate lentiviruses and their more derived primate counterparts. Thus, pSIVgml represents the first unambiguous example of a viral transitional form, revealing the acquisition and loss of genomic features during lentiviral evolution. Furthermore, because terrestrial mammal populations in Madagascar and Africa are likely to have been isolated from one another for at least 14 million years, the presence of pSIVgml in the gray mouse lemur genome indicates that lentiviruses must have been infecting primates for at least this period of time, or have been transmitted between Malagasy and African primate populations by a vector species capable of traversing the Mozambique channel. The discovery of pSIVgml illustrates the utility of endogenous sequences for the study of contemporary retroviruses and indicates that primate lentiviruses may be considerably older and more broadly distributed than previously thought.

It is important to understand how sympatric congeners can co-occur within the same landscapes to better understand niche differentiation and how each species respond to habitat modification. We compiled previously published, long-term data from several studies on encounter rates of two sympatric mouse lemurs (Microcebus ravelobensis and M. murinus) in different survey sites (surveyed portions of continuous forest and individual fragments) using trapping (N = 16 sites; 42 samples) and visual (N = 42 sites; 73 samples) survey methods in Ankarafantsika National Park, Madagascar (total N = 58 sites; 115 samples). A sample reflects multiple surveys, visual, and/or trapping (N = 2–21) along a line-transect and/or trapline within 1 year. We found higher encounter rates of M. ravelobensis and M. murinus in continuous forests and fragmented forests, respectively. Contrary to studies conducted at a smaller scale, yearly encounter rates for M. ravelobensis were negatively associated with rainfall but were positively associated with rainfall for M. murinus. Like previous studies, we found a negative correlation in encounter rates between M. ravelobensis and M. murinus in continuous forests. However, in fragmented forests there was a significant positive relationship between the two species. Our data suggest that M. ravelobensis preferred continuous forest habitat, whereas the congeneric M. murinus preferred fragmented forest habitat. Such results are likely related to species-specific ecological requirements: whereas the continuous forest has conditions that M. ravelobensis finds preferential (i.e., wetter, taller, and denser forests), the forest fragments are drier, habitat that is preferred by M. murinus.

Despite increasing interest, animal personality is still a puzzling phenomenon. Several theoretical models have been proposed to explain intraindividual consistency and interindividual variation in behaviour, which have been primarily supported by qualitative data and simulations. Using an empirical approach, I tested predictions of one main life-history hypothesis, which posits that consistent individual differences in behaviour are favoured by a trade-off between current and future reproduction. Data on life-history were collected for individuals of a natural population of grey mouse lemurs (Microcebus murinus). Using open-field and novel-object tests, I quantified variation in activity, exploration and boldness for 117 individuals over 3 years. I found systematic variation in boldness between individuals of different residual reproductive value. Young males with low current but high expected future fitness were less bold than older males with high current fecundity, and males might increase in boldness with age. Females have low variation in assets and in boldness with age. Body condition was not related to boldness and only explained marginal variation in exploration. Overall, these data indicate that a trade-off between current and future reproduction might maintain personality variation in mouse lemurs, and thus provide empirical support of this life-history trade-off hypothesis.

Mutations are the raw material on which evolution acts, and knowledge of their frequency and genomic distribution is crucial for understanding how evolution operates at both long and short timescales. At present, the rate and spectrum of de novo mutations have been directly characterized in relatively few lineages. Our study provides the first direct mutation-rate estimate for a strepsirrhine (i.e., the lemurs and lorises), which comprises nearly half of the primate clade. Using high-coverage linked-read sequencing for a focal quartet of gray mouse lemurs (Microcebus murinus), we estimated the mutation rate to be among the highest calculated for a mammal at 1.52 × 10–8 (95% credible interval: 1.28 × 10−8–1.78 × 10−8) mutations/site/generation. Further, we found an unexpectedly low count of paternal mutations, and only a modest overrepresentation of mutations at CpG sites. Despite the surprising nature of these results, we found both the rate and spectrum to be robust to the manipulation of a wide range of computational filtering criteria. We also sequenced a technical replicate to estimate a false-negative and false-positive rate for our data and show that any point estimate of a de novo mutation rate should be considered with a large degree of uncertainty. For validation, we conducted an independent analysis of context-dependent substitution types for gray mouse lemur and five additional primate species for which de novo mutation rates have also been estimated. These comparisons revealed general consistency of the mutation spectrum between the pedigree-based and the substitution-rate analyses for all species compared.