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Orchids of Tropical America is an entertaining, informative, and splendidly illustrated introduction to the orchid family for enthusiasts and newcomers seeking to learn about more than 120 widespread orchid genera. Joe E. Meisel, Ronald S. Kaufmann, and Franco Pupulin bring alive the riot of colors, extraordinary shapes, and varied biology and ecology of the principal orchid genera ranging from Mexico and the Caribbean to Bolivia and Brazil. Orchids, likely the most diverse family of plants on earth, reach their peak diversity in the tropical countries of the Western Hemisphere, including, for example, more than 2,500 species in Brazil and 4,000 in Ecuador. The book also highlights reserves in the American tropics where travelers can enjoy orchids in the wild. Whether you journey abroad to see these unique plants, raise them in your home, or admire them from afar, this book offers fascinating insights into the diversity and natural history of orchids. Beyond the plant and flower descriptions, Orchids of Tropical America is packed with informative stories about the ecology and history of each genus. Pollination ecology is given in detail, with an emphasis on how floral features distinctive to the genus are linked to interaction with pollinators. This book also features information on medicinal and commercial uses, notes on the discoverers, and relevant historical data. The easy-to-use identification system permits quick recognition of the most common orchid groups in Central and South America. Genus descriptions are given in plain language designed for a nonscientific audience but will prove highly useful to advanced botanists as well. Descriptions focus on external morphology, and great care has been taken to ensure the guide is useful in the field without reliance on microscopes or dissections. Equally valuable as a field guide, a desktop reference, or a gift, Orchids of Tropical America will make an excellent addition to any orchid lover's library. Visit the website for this book at www.orchidsoftropicalamerica.com. \"The authors of this introductory text are acknowledged experts on tropical American orchids with decades of combined experience of orchids in both the wild and culture. You could not find a better trio of enthusiasts and specialists to introduce you to the wonderful and occasionally bizarre world of tropical American orchids.\"-from the Foreword by Phillip J. CribbOrchids of Tropical America is an entertaining, informative, and splendidly illustrated introduction to the orchid family for enthusiasts and newcomers seeking to learn about more than 120 widespread orchid genera. Joe E. Meisel, Ronald S. Kaufmann, and Franco Pupulin bring alive the riot of colors, extraordinary shapes, and varied biology and ecology of the principal orchid genera ranging from Mexico and the Caribbean to Bolivia and Brazil. Orchids, likely the most diverse family of plants on earth, reach their peak diversity in the tropical countries of the Western Hemisphere, including, for example, more than 2,500 species in Brazil and 4,000 in Ecuador. The book also highlights reserves in the American tropics where travelers can enjoy orchids in the wild.Whether you journey abroad to see these unique plants, raise them in your home, or admire them from afar, this book offers fascinating insights into the diversity and natural history of orchids. Beyond the plant and flower descriptions,Orchids of Tropical America is packed with informative stories about the ecology and history of each genus. Pollination ecology is given in detail, with an emphasis on how floral features distinctive to the genus are linked to interaction with pollinators. This book also features information on medicinal and commercial uses, notes on the discoverers, and relevant historical data.The easy-to-use identification system permits quick recognition of the most common orchid groups in Central and South America. Genus descriptions are given in plain language designed for a nonscientific audience but will prove highly useful to advanced botanists as well. Descriptions focus on external morphology, and great care has been taken to ensure the guide is useful in the field without reliance on microscopes or dissections. Equally valuable as a field guide, a desktop reference, or a gift,Orchids of Tropical America will make an excellent addition to any orchid lover's library.Visit the website for this book at www.orchidsoftropicalamerica.com.

In epiphytic orchids, distinctive groups of fungi are involved in the symbiotic association. However, little is known about the factors that determine the mycorrhizal community structure. Here, we analyzed the orchid mycorrhizal fungi communities associated with three sympatric Cymbidieae epiphytic tropical orchids ( Cyrtochilum flexuosum, Cyrtochilum myanthum , and Maxillaria calantha ) at two sites located within the mountain rainforest of southern Ecuador. To characterize these communities at each orchid population, the ITS2 region was analyzed by Illumina MiSeq technology. Fifty-five mycorrhizal fungi operational taxonomic units (OTUs) putatively attributed to members of Serendipitaceae, Ceratobasidiaceae and Tulasnellaceae were identified. Significant differences in mycorrhizal communities were detected between the three sympatric orchid species as well as among sites/populations. Interestingly, some mycorrhizal OTUs overlapped among orchid populations. Our results suggested that populations of studied epiphytic orchids have site-adjusted mycorrhizal communities structured around keystone fungal species. Interaction with multiple mycorrhizal fungi could favor orchid site occurrence and co-existence among several orchid species.

Vanilla planifolia is the source of the spice vanilla. This study is part of an international initiative to study the biology, including mycorrhizal fungi and cultivation practices of vanilla to improve its production in Mexico. The study focused on documenting mycorrhizal fungal diversity in vanilla. It also provided preliminary data on differences in mycorrhizal fungal communities associated with cultivation practices. A richer mycorrhizal community was observed in vanilla growing in a wild natural farm compared with those from a highly managed farm. Our research provides insights for sustainable vanilla production that can benefit Mexican farming communities. Social Impact Statement Vanilla planifolia is the source of the spice vanilla. This study is part of an international initiative to study the biology, including mycorrhizal fungi and cultivation practices of vanilla to improve its production in Mexico. The study focused on documenting mycorrhizal fungal diversity in vanilla. It also provided preliminary data on differences in mycorrhizal fungal communities associated with cultivation practices. A richer mycorrhizal community was observed in vanilla growing in a wild natural farm compared with those from a highly managed farm. Our research provides insights for sustainable vanilla production that can benefit Mexican farming communities. Summary Relatively little is known regarding differences in root symbionts (i.e., mycorrhizae) between epiphytic and terrestrial orchids. We characterized the mycorrhizal fungal communities of aerial and terrestrial roots of the orchid, Vanilla planifolia, from four Mexican farms representing different management systems. Amplicon sequencing identified 40 putative mycorrhizal fungi based on ITS sequence data, these included traditional orchid mycorrhizal fungi such as Ceratobasidium, and Thanatephorus in the order Cantharellales as well as Serendipitaceae in the order Sebacinales, and species of several genera traditionally considered as ectomycorrhizal fungi. Mycorrhizal fungal communities were similar in aerial and terrestrial roots, but differed in read abundances. Plants growing in wild‐natural conditions hosted a richer, but not statistically different, community of mycorrhizal fungi in comparison with plants in the highly managed farm. Soil characteristics including texture, organic matter, N, P, and K do not explain differences between fungal communities at these farms. This is one of the first reports of a diverse community of fungi traditionally considered to form ectomycorrhizas in association with aerial orchid roots. Further research is needed to understand the functional role of these putative mycorrhizal fungi in the ecology of V. planifolia, and if ectomycorrhizal fungi commonly occur in other hemiepiphytic and epiphytic orchids.

A review of comparative levels of reproductive success among nectariferous and nectarless orchids worldwide was compiled from a comprehensive survey of fruit set from 117 orchid species in the literature and from our own field studies. It confirms the hypothesis that nectariferous orchids are more successful in setting fruit than are nectarless species. Overall fruit set figures for nectarless and nectariferous orchids were 19.5 and 49.3% for North America, 27.7 and 63.1% for Europe. 41.4 and 74.4% for the temperate southern hemisphere, and 11.5 and 24.9% for the tropics, demonstrating that the dichotomy is consistent across all geographical areas. On average, the provision of nectar doubles the probability of fruit set in both temperate and tropical areas, but tropical orchids are remarkable in that all (whether nectarless or nectariferous, or terrestrial or epiphytic) display low fruit productivity (<50%). Fruiting failure in the tropics may be balanced by higher productivity per capsule. since tropical orchid fruits contain on average 150 times more seeds than temperate ones. Hybridization occurs more frequently among nectarless orchids in Britain and Europe than among nectariferous ones, and there is a significant positive association between orchid rarity and lack of nectar reward in the British Isles. Sexual reproduction in the Orchidaceae is predominantly pollinator dependent, but this can sometimes be successfully circumvented by asexual seed production (agamospermy) or. more frequently, by automatic self-pollination (autogamy). The proportion of highly successful nectarless orchids from all geographic areas is very low and comparable with that of orchids offering rewards other than nectar (approximately 14% of species in each case) emphasizing that high reproductive success is only associated with nectar reward (53% of species). It is suggested that the evolution of nectar production within the family has been the most frequent means of escaping the reproductive limitations of low pollinator visitation frequencies.

In this study, we isolate and analyse a new set of microsatellite loci for Cattleya walkeriana. Twenty-two primer pairs were screened for C. walkeriana (n = 32) and assessed for their transferability to Cattleya loddigesii (n = 12) and Cattleya nobilior (n = 06). All loci amplified for C. walkeriana; however, for C. loddigesii and C. nobilior, four and five primers, respectively, did not present amplification. The polymorphic loci presented between 2 and 13 alleles per locus for both C. walkeriana and C. loddigesii, with respective averages of 5.1 and 4.2. For C. nobilior, we found between two and five alleles per locus, with an average of 2.6. For C. walkeriana, observed heterozygosity varied from 0.100 to 0.966, whereas expected heterozygosity ranged from 0.097 to 0.900. The observed and expected heterozygosity for C. loddigesii and C. nobilior were also estimated. We found no significant linkage disequilibrium between any pair of loci, and evidence of null alleles at four loci (Cw16, Cw24, Cw30 and Cw31) for C. walkeriana. The combined power to exclude the first parent and combined non-exclusion probability of identity were 0.999 and 2.3 × 10−20, respectively. These new loci can be used in studies of germplasm resources, and assessments of genotypic and genetic diversity and population structure, thus improving the accuracy of such analyses and their applicability in the conservation and protection of these endangered species.

Cattleya walkeriana, one of the most improved Brazilian Cattleyas, is a popular tropical orchid endemic from Brazil and currently endangered. In the present study, for the first time microsatellite markers were developed for C. walkeriana and their transferability was tested for the species C. loddigesii and C. nobilior. The markers were used for genotyping 26 C. walkeriana specimens from different growers and from different levels of improvement. The transferability was successful, with five polymorphic loci transferred to C. loddigesii and six polymorphic loci to C. nobilior. Eight loci were polymorphic, revealing a maximum of two to ten alleles per locus in C. walkeriana and two to four and two to five in C. loddigesii and C. nobilior, respectively. There was no significant linkage disequilibrium in the studied loci. For C. walkeriana, the observed and expected heterozygosities ranged from 0 to 0.963 and from 0.138 to 0.841, respectively. These markers identified polymorphisms and may be used to study the genetic diversity, gene flow or hybridization of these species.

Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index (FIS) ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations.

We have successfully developed a method to induce early in vitro flowering of the self-pollinated seedlings of a tropical orchid hybrid, Dendrobium Madame Thong-In. Transition of vegetative shoot apical meristem to inflorescence meristem was observed when young protocorms were cultured in modified KC liquid medium. In contrast, protocorms cultured on Gelrite-solidified medium only produced axillary shoots and roots. CW was required to trigger the transitional shoot apical meristem and BA enhanced inflorescence stalk initiation and flower bud formation. However, normal flower development was deformed in liquid medium but developed fully upon transferring to two-layered (liquid over Gelrite-solidified) medium. Under optimal condition, in vitro flowering was observed about 5 months after seed sowing. Segregation of flower colours was observed in these seedlings and seedpods formed upon artificial pollination of the in vitro flowers.

Geodorum (Orchidaceae) has approximately ten species, and is mainly distributed in tropical Asia. Among them, Geodorum eulophioides Schltr. is of major conservation concern as it has only four known populations, two each found in China and others in Burma. In this study, we developed 18 polymorphic microsatellites markers in G. eulophioides with the number of alleles ranging from two to 17. The expected and observed heterozygosities ranged from 0.046 to 0.918 and from 0.048 to 1.0000, respectively. The cross-species amplification rates for the sympatric G. recurvum (Roxb.) Alston and G. densiflorum (Lam.) Schltr. were 100 and 83.33 %, respectively. These markers developed would be useful for conservation and population genetic studies.