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Nucleic acid amplification testing has great potential for point-of-need diagnostic testing with high detection sensitivity and specificity. Current sample preparation is limited by a tedious workflow requiring multiple steps, reagents and instrumentation, hampering nucleic acid testing at point of need. In this study, we present the use of mixed cellulose ester (MCE) paper for DNA binding by ionic interaction under molecular crowding conditions and fluid transport by wicking. The poly(ethylene) glycol-based (PEG) reagent simultaneously provides the high pH for alkaline lysis and crowding effects for ionic binding of the DNA under high salt conditions. In this study, we introduce Paper-based Abridged Solid-Phase Extraction with Alkaline Poly(ethylene) Glycol Lysis (PASAP). The anionic mixed cellulose ester (MCE) paper is used as solid phase and allows for fluid transport by wicking, eliminating the need for pipetting skills and the use of a magnet to retain beads. Following the release of DNA from the cells due to the lytic activity of the PASAP solution, the DNA binds to the anionic surface of the MCE paper, concentrating at the bottom while the sample matrix is transported towards the top by wicking. The paper was washed by dipping it in 40% isopropanol for 10 s. After air-drying for 30 s, the bottom section of the paper (3 mm × 4 mm) was snapped off using the cap of a PCR tube and immersed in the colourimetric loop-mediated isothermal amplification (cLAMP) solution for direct amplification and colourimetric detection. The total sample processing was completed in 15 min and ready for amplification. cLAMP enabled the detection of 10 2 CFU/mL of Escherichia coli ( E. coli ) from culture media and the detection of E. coli in milk < 10 3  CFU/mL (10 CFU) after incubation at 68 °C for 60 min, demonstrating applicability of the method to complex biological samples.

Empowering aboriginal people to police themselves will help make Canada a greater society. This is just one small step in allowing its aboriginal communities to take their rightful place amongst the collage of beautiful colors that make up our beautiful country.

On the day of the assault, police told the media that Pasap had asked to go to a specific area of the city and became combative when he was told the bus route didn't go to that location.

In junior boys large school action: Campbell beat O'Neill 75-68, Balfour beat Riffel 89-77, Sheldon topped Miller 73-68 and Luther beat the Winston Knoll Wolverines 78-57. The group was then invited to compete at the Elite Canada competition over the weekend where [Sarah-Jane Laxdal] was 17th, [Jennifer Levasseur] was 20th and [Emily Thomson] was 21st in the senior all-around division. Regina's Emilie Chan was 21st in the junior all-around.

The National Indian Gaming Alliance was formed to help Indian bands establish and run casinos on reserves across the country, said Ed Pasap of Saskatchewan's White Bear band. Mr. Pasap said the alliance includes 15 Indian bands from British Columbia, Saskatchewan, Alberta, Manitoba, Ontario, Nova Scotia, New Brunswick and Quebec, and has been endorsed by the Assembly of First Nations.

Denise Faye Ryder, 33, made her first appearance on the murder charge Monday. She had been in custody since her arrest Saturday, the same day police were called to a North-Central home and found the body of Richard Scott Pasap. Provincial Court Judge Dennis Fenwick ordered Ryder to remain in custody overnight and to come back to court Tuesday morning. Ryder was supposed to be in Provincial court today anyway. \"She was charged with attempting to murder Richard Pasap,\" Crown prosecutor Jeff Kalmakoff told The Leader-Post Monday.

The city's first homicide of 2000 occurred on Feb. 26 when 18- year-old Lisa Faye Sheepskin was stabbed to death inside 1302 Angus St. during an altercation over money. Arnold James Dufour, 30, pleaded guilty to manslaughter and was sentenced to four years in prison. [Dave Wyatt] was uncertain of the name of the city's fourth homicide victim, saying the death was originally ruled accidental then recently upgraded to a homicide. Anyone with further information about this latest homicide is asked to call Regina police at 777-6500 or Crime Stoppers at 545- TIPS (545-8477).

[Frank Impey] said a fight broke out between Cote and [Morley Allan Pasap], with Cote getting the worst of it. (Earlier this week, Pasap pleaded guilty to assault causing bodily harm of Cote and was given a conditional sentence on top of five and half months' time served.) Cote fell unconscious, but when he came to, he began threatening his wife and again arguing with Pasap. That's when Favel stabbed him. \"She thought she was protecting her sister,\" [Ellen Gunn] said. In sentencing Favel, Gunn noted her \"very difficult life.\" She was placed in an Indian residential boarding school at the age of six. From Grades 4 to 8, she attended school in Regina where she was bullied and made to feel less important than white people. She was physically and sexually abused at age nine, and spent ages 10 to 14 bouncing around foster homes. By age 17, Favel was an alcoholic and homeless. She gave birth to six children, two of whom were placed for adoption. Favel also lost three siblings to suicide and one to freezing.