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The fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) has successfully invaded Africa, where it has significantly impacted maize and sorghum production. Management of FAW in Africa predominantly relies on synthetic insecticides, which are expensive, and negatively impact the environment and beneficial insects. We, therefore, conducted field surveys in Uganda in 2017 and 2019 to identify egg and larval parasitoids of FAW for possible use in integrated pest management (IPM) programs. Parasitoids were identified by their mitochondrial DNA cytochrome c oxidase subunit 1 (mtCOI) gene sequences. We identified 13 parasitoid species belonging to three families of Hymenoptera: Platygastridae, Braconidae and Ichneumonidae, as well as one Dipteran family (Tachinidae). Coccygidium spp. and Chelonus bifoveolatus were the most abundant and widely distributed parasitoids. Overall, parasitism averaged 9.2% and ranged from 3.1% to 50% in 2017, and 0.8% to 33% in 2019. Parasitism by Sturmiopsis parasitica, Diolcogaster sp., and Cotesia flavipes on FAW in maize crops are reported for the first time. Our results suggest high biological diversity of FAW parasitoids, which should be exploited in the IPM of the FAW in Uganda.

The study of the technical and economic aspects of rearing natural enemies is essential for its effective use as a biological control agent in the field.The aim of this study was to determine the cost of production of the parasitoid, Telenomus remus (Nixon; Hymenoptera: Platygastridae), reared in eggs of its natural host, Spodoptera frugiperda (J. E. Smith; Lepidoptera: Noctuidae), and the alternative host, Corcyra cephalonica (Stainton; Lepidoptera: Pyralidae). The insects were reared in accordance with their respective methodologies of laboratory rearing. The cost of production of this biocontrol agent is US$ 0.0004 when reared with S. frugiperda eggs and US$ 0.0002 with C. cephalonica eggs.

Synopeas maximum Awad & Talamas, sp. nov. , the first reported parasitoid associated with the soybean gall midge, Resseliella maxima Gagné, is described based on morphological and molecular data. Parasitoids were reared from soybean stems infested by R. maxima in Minnesota. A phylogenetic reconstruction of the genus Synopeas Förster was performed with COI sequences (n=2412) available on the Barcode of Life Data System (BOLD). Phylogenetic and barcode gap analyses suggest 279 Synopeas species in this dataset, with S. maximum sequences forming a monophyletic clade that is distinct from relatives. The Synopeas maximum clade was close to specimens from Canada and the United States, suggesting it is native to North America. We present a taxonomic treatment of S. maximum to facilitate its identification, including comparison to morphologically similar species. This project provides baseline data for further ecological study of R. maxima parasitism, and its management in soybean.

Spodopotera frugiperda is a worldwide invasive pest that has caused significant economic damage. According to the classical biological control approach, natural enemies that can control invasive pests come from the same area of origin as the pests that have experienced coadaptation processes. However, the new association’s approach suggests that local natural enemies are equally capable of controlling invasive pests. Due to the lack of data on the association of S. frugiperda and local natural enemies, research was conducted through a rapid survey to study the diversity of parasitoids associated with S. frugiperda. The results showed 15 parasitoid species associated with S. frugiperda. Four egg parasitoids, eight larval parasitoids, and three larval–pupal parasitoids were found to be associated with S. frugiperda for three years after it was first discovered in Indonesia. Eleven of them are new reports of parasitoids associated with S. frugiperda in Indonesia. A new association was found between S. frugiperda and twelve parasitoid species, consisting of three egg parasitoids (Platygasteridaesp.01, Platygasteridaesp.02, and Telenomus remus), six larval parasitoids (Apanteles sp., Microplitis sp., Campoletis sp., Coccygidium sp., Eupelmus sp., and Stenobracon sp.), and three larval–pupal parasitoids (Brachymeria lasus, B. femorata, and Charops sp.). Telenomus remus is the most dominant parasitoid, with a higher abundance and parasitism rate. The result suggests another method for selecting biological control using the new association approach since local natural enemies can foster quick adaptation to invasive pests.

The egg parasitoid Trissolcus vassilievi (Mayr) is a key natural enemy of sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae).This laboratory study examined how the aggregation of parasitoids varied in response to host density and numbers of foraging conspecifics. Five host densities (1, 2, 4, 8, and 15 clutches of E. integriceps eggs), were offered simultaneously to 1, 2, 4, 8, or 16 female wasps in a standardized arena (30 cm diameter) and patch residence times were recorded. Patch residence time was strongly correlated with parasitism, and increased as the number of host clutches increased up to eight, but declined when more clutches were offered. Wasps displayed low mobility and tended to remain in the patch initially encountered, even when it contained few egg masses. At higher wasp densities, patches were occupied more quickly, host exploitation began sooner, and per capita handling time was reduced. However, total patch residence times were similar across all densities. Thus, females responded to conspecifics with increased movement, which increased dispersal and resulted in a more homogeneous distribution of wasps among patches. Pseudo-interference, resulting from wasps remaining in some patches and neglecting others, had greater impact on final levels of host exploitation than did actual interference (patch abandonment following conspecific encounters).These phenomena resulted in decreased parasitoid searching rates and a 2-fold increase in host survival at higher wasp densities.

Despite the economic importance of Theobroma cacao, surprisingly little is known about its pollination ecology. Ceratopogonid midges are considered to be the main pollinators, but the limited available evidence on the sexual reproduction is based almost exclusively on cultivated cacao and knowledge is nonexistent for wild populations. We documented flower visitors in wild and cultivated plants by applying glue on 2237 flowers of wild and cultivated cacao trees in Bolivia to trap floral visitors. We caught 631 insects belonging to seven orders, corresponding to a mean capture rate of 0.3 insects per flower. The most abundant and diverse insect order on both cacao types was Hymenoptera, represented mainly by small parasitoids. Hymenoptera were more abundant on wild cacao, whereas species richness was higher on cultivated cacao. The abundance and species richness of Diptera were not significantly different between wild and cultivated cacao. However, species composition and proportion of Diptera species differed between both wild and cultivated cacao. Ceratopogonidae were only represented by 13 individuals belonging to seven species. Cacao pollen was carried by only a single specimen of Encyrtidae. We were thus unable to identify actual pollinators. We found significant differences among the visitor assemblages between wild and cultivated cacao, which suggest that midges alone were probably too rare to act as main or even sole pollinators of cacao in our study region. Potential additional pollinators would be small Diptera (e.g., Chloropidae and Phoridae) and Hymenoptera (e.g., Eulophidae and Platygastridae).

Gall cytological and histochemical features established by the constant feeding activity of the associated gall inducer may be changed due to the attack of parasitoids. We accessed two tri-trophic systems involving the globoid bivalve-shaped gall on Mimosa gemmulata Barneby (Fabaceae) and its galling undescribed species of Lopesia (Diptera: Cecidomyiidae), which may be ectoparasitized by Torymus sp. (Hymenoptera: Torymidae) or endoparasitized by a polyembryonic Platygastridae (Hymenoptera), as models of study. The ectoparasitoid species paralyzes and kills Lopesia sp. larva, which stops the feeding stimuli, while the endoparasitoid larvae feed in Lopesia sp. larva body and keep it alive for a certain time. Our hypothesis is that the time lapse of Lopesia sp. feeding impairment by the two parasitoids will cause distinct cytological and histochemical responses in the ecto- and endoparasitized galls compared to the non-parasitized condition. In both parasitoidism cases, the impairment of the feeding activity of the galling Lopesia sp. directs the common storage and nutritive cells toward a similar process of induced cell death, involving cell collapse and loss of membrane integrity. The cell metabolism is maintained mainly by mitochondria, and by the translocation of lipids from the common storage tissue, via plasmodesmata, through the living sclereids of the mechanical zone toward the nutritive tissue. Accordingly, the parasitoid impairment on the feeding activity of Lopesia sp. larvae causes precocious senescence, but similar cytological alterations, and no impact over the histochemical profiles, regarding lipids, reactive oxygen species, and secondary metabolites, which support gall metabolism along the parasitoid cycles.

We report the results of DNA barcoding week-long Malaise trap catches from 11 sites in three Thai conservation areas, concentrating on the parasitoid Hymenoptera, particularly the superfamily Ichneumonoidea. From a total of 15,338 parasitoid wasp sequences, 13,473 were barcode compliant and could be assigned to a family based on morphology and sequence data. These collectively represented 4917 unique BINs (putative species) in 46 families, with the Scelionidae, Ichneumonidae, Eulophidae, Braconidae and Platygastridae being, by far, the most abundant. Spatial proximity had a strong positive effect on the numbers of BINs shared between traps.

Platygastridae (Hymenoptera) is a diverse family of parasitoid wasps for which few studies of internal morphology have been conducted. The monophyly of the group is undisputed based on recently published molecular data, but based on morphology, the family is diagnosable from other platygastroids only by a combination of character reductions. In the present study we explored the mesosoma of Platygastroidea and found two new synapomorphies for Platygastridae: an externally visible anterior mesofurcal pit, which corresponds to an invagination that connects to the anterior portion of the mesofurca, and internally, a posteriorly shifted origin of the first wing flexors. The absence of a mesofurcal bridge and the exclusively mesopectal origin of the fore wing flexors are treated as synapomorphies for Platygastridae+Janzenellidae. Phylogenetic implications and evolutionary hypotheses regarding these traits are discussed.

Proterosceliopsis was erected by Ortega-Blanco et al. (2014) in their treatment of scelionid genera in Cretaceous amber from Álava, Spain. The generic description appears to have been based on specimens in which only the dorsal aspects of the mesosoma and metasoma were visible, as characters of the mesopleuron, metapleuron, lateral pronotum, and ventral metasoma were not mentioned. We here provide a comprehensive description of the genus that includes characters from throughout the body and we reinterpret some of the characters presented by Ortega-Blanco et al. (2014). Our analysis of Proterosceliopsis in the context of extant and fossil platygastroids places this group as a lineage well outside of the current families. We here designate Proterosceliopsis as the type genus of a new family, Proterosceliopsidae Talamas, Johnson, Shih & Ren, fam. nov. , and describe five new species: Proterosceliopsis ambulata Talamas, Shih & Ren, sp. nov. , P. nigon Talamas, Shih & Ren, sp. nov. , P. plurima Talamas, Shih & Ren, sp. nov. , P. torquata Talamas, Shih & Ren, sp. nov. , and P. wingerathi Talamas, Shih & Ren, sp. nov. We provide a key to all presently known species in the genus. The oldest known specimen of Platygastridae s.s., in Burmese amber, is presented and compared to Proterosceliopsidae fam. nov.