Explore the vast range of titles available.

Freshness is one of the most important parameters for assessing the quality of avian eggs. Available techniques to estimate the degradation of albumen and enlargement of the air cell are either destructive or not suitable for high-throughput applications. The aim of this research was to introduce a new approach to evaluate the air cell of quail eggs for freshness assessment as a fast, noninvasive, and nondestructive method. A new methodology was proposed by using a thermal microcamera and deep learning object detection algorithms. To evaluate the new method, we stored 174 quail eggs and collected thermal images 30, 50, and 60 days after the labeled expiration date. These data, 522 in total, were expanded to 3610 by image augmentation techniques and then split into training and validation samples to produce models of the deep learning algorithms, referred to as “You Only Look Once” version 4 and 5 (YOLOv4 and YOLOv5) and EfficientDet. We tested the models in a new dataset composed of 60 eggs that were kept for 15 days after the labeled expiration label date. The validation of our methodology was performed by measuring the air cell area highlighted in the thermal images at the pixel level; thus, we compared the difference in the weight of eggs between the first day of storage and after 10 days under accelerated aging conditions. The statistical significance showed that the two variables (air cell and weight) were negatively correlated (R2 = 0.676). The deep learning models could predict freshness with F1 scores of 0.69, 0.89, and 0.86 for the YOLOv4, YOLOv5, and EfficientDet models, respectively. The new methodology for freshness assessment demonstrated that the best model reclassified 48.33% of our testing dataset. Therefore, those expired eggs could have their expiration date extended for another 2 weeks from the original label date.

This study was planned to evaluate the impact of different nano-curcumin levels on the growth rate, carcass, blood chemistry and caecal microbes of growing quail. A total of 270 Japanese quails at one-week-old were distributed to six equal groups; each group consisted of 45 unsexed birds with five replications (nine quails each). The 1st group was fed a basal diet, whereas the 2nd, 3rd, 4th, 5th and 6th groups were fed diets containing nano-curcumin (0.1, 0.2, 0.3, 0.4 and 0.5 g/kg diet, respectively). Nano-curcumin levels significantly increased (p ≤ 0.0001) body weight at 3 weeks and 5 weeks of age. Body weight gain during 1–3, 3–5 and 1–5 weeks of age was significantly increased (p < 0.0001) in groups treated with nano-curcumin levels (except at 0.3 g/kg; 1–3 weeks) compared to control. During 1 to 5 weeks, feed intake was decreased (p < 0.0001) in birds receiving nano-curcumin (0.1, 0.3 and 0.4 g/kg) diets. The best values of feed conversion ratio were recorded for the 0.4 g nano-curcumin-treated group. Carcass traits were not affected Nano-curcumin levels. The inclusion of nano-curcumin (0.2, 0.3 or 0.5 g/kg) significantly increased serum TP (p = 0.0004), albumin (p = 0.0078) and globulin (p < 0.0001). Quails fed with nano-curcumin (0.2 g/kg) exhibited the highest SOD and GSH activities, serum IgG and IgM concentrations and complement values compared to control. The addition of any level of nano-curcumin in the quail diet also significantly improved the lipid profile. In conclusion, supplemental nano-curcumin had beneficial impacts on growth, lipid profile, blood constituents, antioxidant indices, and immunity of growing quail, as well as increasing counts of lactic acid bacteria and reducing pathogenic bacteria.

Tissue morphogenesis is driven by local cellular deformations that are powered by contractile actomyosin networks. How localized forces are transmitted across tissues to shape them at a mesoscopic scale is still unclear. Analyzing gastrulation in entire avian embryos, we show that it is driven by the graded contraction of a large-scale supracellular actomyosin ring at the margin between the embryonic and extraembryonic territories. The propagation of these forces is enabled by a fluid-like response of the epithelial embryonic disk, which depends on cell division. A simple model of fluid motion entrained by a tensile ring quantitatively captures the vortex-like “polonaise” movements that accompany the formation of the primitive streak. The geometry of the early embryo thus arises from the transmission of active forces generated along its boundary.

Many species, including humans exhibit a wide range of social behaviors that are crucial for the adaptation and survival of most species. Brain organization and function are shaped by genetic and environmental factors, although their precise contributions have been relatively understudied in the context of artificial selection. We used divergent lines of quail selected on their high versus low level of motivation to approach a group of conspecifics (S + and S-, respectively) to investigate the influence of genetic selection and early social environment on sociability. We observed distinct sex-and brainregion-specific expression patterns of three neuronal markers: mesotocin, and vasotocin, the avian homologues of mammalian oxytocin and vasopressin, as well as aromatase, the enzyme that converts androgens into estrogens. These markers displayed pronounced and neuroanatomically specific differences between S + and S-quail. Additionally, in a second experiment, we assessed the influence of early social environment on social skills in juvenile birds. Mixing S + and S-resulted in more S-males approaching the group without affecting the sociability of S + or other behaviors, suggesting that the early social environment may influence the results of genetic selection. In conclusion, the divergent quail lines offer a valuable model for unraveling the neuronal and behavioral mechanisms underlying social behaviors.

Poultry production utilizes many available technologies in terms of farm-industry automation and sanitary control. However, there is a lack of robust techniques and affordable equipment for avian embryo detection and sexual segregation at the early stages. In this work, we aimed to evaluate the potential use of thermal micro cameras for detecting embryos in quail eggs via thermal images during the first 168 h (7 days) of incubation. We propose a methodology to collect data during the incubation period. Additionally, to support the visual analysis, YOLO deep learning object detection algorithms were applied to detect unfertilized eggs; the results showed its potential to distinguish fertilized eggs from unfertilized eggs during the incubation period, after filtering radiometric images. We compared YOLOv4, YOLOv5 and SSD-MobileNet V2 trained models. The mAP@0.50 of the YOLOv4, YOLOv5 and SSD-MobileNet V2 was 98.62%, 99.5% and 91.8%, respectively. We also compared three testing datasets for different intervals of rotation of eggs, as our hypothesis was that fewer turning periods could improve the visualization of fertilized egg features, and applied three treatments: 1.5 h, 6 h, and 12 h. The results showed that turning eggs in different periods did not exhibit a linear relation, as the F1 Score for YOLOv4 of detection for the 12 h period was 0.569, that for the 6 h period was 0.404 and that for the 1.5 h period was 0.384. YOLOv5 F1 Scores for 12 h, 6 h and 1.5 h were 1, 0.545 and 0.386, respectively. SSD-MobileNet V2 performed F1 scores of 0.60 for 12 h, 0.22 for 6 h and 0 for 1.5 h turning periods.

The primary driver of nest failure for terrestrial birds is depredation, especially for ground-nesting species that arc vulnerable to a diverse guild of predators. However, descriptions of how complex predator-prey interactions happen, and ultimately lead to nest failures, are scarce. Herein, we provide observations collected from cameras stationed at nest sites as part of a larger study on Northern Bobwhite (Colinus virginianus) and Scaled Quail (Callipepla squamata) nesting behavior in the Oklahoma Panhandle during 2016. We observed 2 multi-episode (i.e.. multiple predator visits) diurnal nest depredation events at a nest of each species. In both instances, repeated nest depredation by hispid cotton rats (Sigmodon kispidus) as well as 2 different snake species resulted in nest abandonment. We observed that cotton rats routinely visited nest sites (up to 11 times in 1 diurnal period), and often entered the nest during oft' bouts (64% of off bouts) of the incubating females. By detailing interspecific interactions involved in nest depredation. we provide a glimpse into the complexity of the nesting ecology of ground-dwelling birds. These observations further reinforce the potential pitfalls of categorizing nest depredation events without camera data. Received 17 March 2022. Accepted 17 September 2022.

The present work was carried out to investigate the effects of dietary propolis supplementation to laying Japanese quail (Coturnix coturnix japonica) on egg production, egg quality, physiological and immunological aspects under heat stress conditions. A total of 200, 21-day-old, Japanese quail females were distributed equally into standard wired cages in two identical environmentally-controlled rooms (10 cages per room, 10 birds per cage). From 29-70 d of age, the quail birds in the first room remained at a normal temperature of 24°C (C group), whereas the quail birds in the second room were kept under heat stress at 35°C (HS group). Each group was further assigned to 2 propolis subgroups (5 cages per subgroup); one of them received a basal diet without propolis supplementation (-PR subgroup), while, the other received 1 g propolis/ kg basal diet (+PR subgroup). In the present study, performance and egg production of laying quail were significantly (P<0.001) impaired by HS treatment and improved by the PR treatment. Similarly, the negative and positive effects of HS and PR, respectively, were appeared on the egg shell thickness and yolk index. Stress indicators in laying quail were significantly (P<0.001) increased by HS, while, PR significantly (P<0.05) moderated these levels in the HS+PR group when compared to the HS-PR quail group. In addition to the positive impact of PR on the plasma levels of calcium, phosphorus, and albumin, it also normalized the plasma levels of alanine aminotransferase and cholesterol in the heat-stressed quail birds. Moreover, the quail birds in the HS groups expressed lower immunological aspects than those in the C group, while, the addition of propolis to the diets enhanced the immune status of laying quail birds under HS conditions. These results strongly suggest that dietary propolis supplementation could be a successful attempt to maintain the performance and egg production of laying Japanese quail at convenient levels under heat stress conditions.

The quail populations are considered to be one of the largest among the poultry species. Although quail egg and meat production are growing rapidly, still, quail farming practices and welfare aspects are not well established. Stocking density is one of the factors that can significantly affect the welfare, health, and performance of birds, but according to The Expert Group for Technical Advice on Organic Production, no allowed space standards have been reported for new hybrids and different breeds of quail. This review presents an overview of 1) the benefits of egg and meat of quail, 2) the global market of quail products, 3) the factors affecting the stocking density rate, and 4) the effects of stocking density rates and housing systems on the social behavior, welfare, physiological indices, and performance parameters of broiler and laying quail. Conclusively, larger space and enriched aviaries reduce aggressive behavior, and improve the quail welfare and immunological indices. However, the effect of stocking density on some blood biochemical indices and growth performance parameters showed mixed results. A better understanding of the relationship between housing, health, growth performance, and welfare aspects would assist in the implementation of welfare-economic standards for quail production. According to available data, stocking density ranges for broiler and laying Japanese quail are suggested; however, these stocking rates should be tested under different conditions.

Background The Japanese quail (Coturnix japonica) is a popular domestic poultry species and an increasingly significant model species in avian developmental, behavioural and disease research. Results We have produced a high-quality quail genome sequence, spanning 0.93 Gb assigned to 33 chromosomes. In terms of contiguity, assembly statistics, gene content and chromosomal organisation, the quail genome shows high similarity to the chicken genome. We demonstrate the utility of this genome through three diverse applications. First, we identify selection signatures and candidate genes associated with social behaviour in the quail genome, an important agricultural and domestication trait. Second, we investigate the effects and interaction of photoperiod and temperature on the transcriptome of the quail medial basal hypothalamus, revealing key mechanisms of photoperiodism. Finally, we investigate the response of quail to H5N1 influenza infection. In quail lung, many critical immune genes and pathways were downregulated after H5N1 infection, and this may be key to the susceptibility of quail to H5N1. Conclusions We have produced a high-quality genome of the quail which will facilitate further studies into diverse research questions using the quail as a model avian species.

Early amniote development is highly self-organized, capable of adapting to interference through local and long-range cell–cell interactions. This process, called embryonic regulation 1 , has been well illustrated in experiments on avian embryos, in which subdividing the epiblast disk into different parts not only redirects cell fates to eventually form a complete and well-proportioned embryo at its original location, but also leads to the self-organization of additional, fully formed embryos 2 , 3 in the other separated parts. The cellular interactions underlying embryonic self-organization are widely believed to be mediated by molecular signals, yet the identity of such signals is unclear. Here, by analysing intact and mechanically perturbed quail embryos, we show that the mechanical forces that drive embryogenesis self-organize, with contractility locally self-activating and the ensuing tension acting as a long-range inhibitor. This mechanical feedback governs the persistent pattern of tissue flows that shape the embryo 4 – 6 and also steers the concomitant emergence of embryonic territories by modulating gene expression, ensuring the formation of a single embryo under normal conditions, yet allowing the emergence of multiple, well-proportioned embryos after perturbations. Thus, mechanical forces act at the core of embryonic self-organization, shaping both tissues and gene expression to robustly yet plastically canalize early development. Mechanical forces act at the core of bird embryonic self-organization, shaping both tissues and gene expression to robustly yet plastically canalize early development.