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The extreme conditions in arid ecosystems make these environments sensitive to environmental changes. Particularly, land use and seasonal changes are determinants of their soil carbon dynamics. The effect of those elements on soil respiration (RS) is still poorly known in several arid regions of the world. This study investigates the seasonal effect on the RS and its controlling factors throughout different land use systems in northeastern Mexico. RS and 34 biotic and abiotic variables were measured across agricultural crops, natural shrublands, livestock farms, walnut orchards, and industrially influenced soils during the dry and wet seasons. Six variables (soil water content, soil organic matter, soil temperature, silt, and pH) were found as drivers of RS on both local and regional scales. Seasonal and land use had a transversal effect on RS and its controlling factors. RS dynamics were primarily modulated by soil water content, with the wet season and managed lands showing increased sensitivity to climatic and anthropogenic changes. These results indicate that land management strategies are critical for carbon cycling, particularly in water-limited regions like northeastern Mexico, where land use changes are occurring at an accelerated pace.