Explore the vast range of titles available.

Background Fragaria nilgerrensis (FN) provides a rich source of genetic variations for strawberry germplasm innovation. The color of strawberry fruits is a key factor affecting consumer preferences. However, the genetic basis of the fruit color formation in F. nilgerrensis and its interspecific hybrids has rarely been researched. Results In this study, the fruit transcriptomes and flavonoid contents of FN (white skin; control) and its interspecific hybrids BF1 and BF2 (pale red skin) were compared. A total of 31 flavonoids were identified. Notably, two pelargonidin derivatives (pelargonidin-3-O-glucoside and pelargonidin-3-O-rutinoside) were revealed as potential key pigments for the coloration of BF1 and BF2 fruits. Additionally, dihydroflavonol 4-reductase ( DFR ) (LOC101293459 and LOC101293749) and anthocyanidin 3-O-glucosyltransferase ( BZ1 ) (LOC101300000), which are crucial structural genes in the anthocyanidin biosynthetic pathway, had significantly up-regulated expression levels in the two FN interspecific hybrids. Moreover, most of the genes encoding transcription factors (e.g., MYB, WRKY, TCP, bHLH, AP2, and WD40) related to anthocyanin accumulation were differentially expressed. We also identified two DFR genes (LOC101293749 and LOC101293459) that were significantly correlated with members in bHLH, MYB, WD40, AP2, and bZIP families. Two chalcone synthase ( CHS ) (LOC101298162 and LOC101298456) and a BZ1 gene (LOC101300000) were highly correlated with members in bHLH, WD40 and AP2 families. Conclusions Pelargonidin-3-O-glucoside and pelargonidin-3-O-rutinoside may be the key pigments contributing to the formation of pale red fruit skin. DFR and BZ1 structural genes and some bHLH, MYB, WD40, AP2, and bZIP TF family members enhance the accumulation of two pelargonidin derivatives. This study provides important insights into the regulation of anthocyanidin biosynthesis in FN and its interspecific hybrids. The presented data may be relevant for improving strawberry fruit coloration via genetic engineering.

Delayed greening of young leaves is a ubiquitous and visually striking phenomenon in the tropics. Here, we investigated the potential ecological functions of red coloration patterns in young leaves. To detect any protective function of the red coloration on the young leaves, leaf damage by insect herbivores was recorded in the field. To determine capacity for chemical defense, the concentrations of tannins and anthocyanins were measured in both young and mature leaves. To test the hypothesis that anthocyanins function as photo-protective molecules, chlorophyll content, maximum photochemical efficiency of ( / ), non-photochemical quenching ( ), and effective quantum yield of ( ) were measured in the field. Phylogenetic relationships were analyzed to test the relationary significance of the occurrence of redness in young leaves. Compared to the coloration in non-red leaves, young red leaves had significant higher anthocyanins and tannins content and lower herbivore damages. Young, red leaves had the lowest / values, which were significantly lower than those of non-red leaves. values in young red leaves were comparable to those of other groups. Although young red leaves had high , these values were significantly lower than those of the other three groups. The results suggest that the red coloration of young leaves protects them from insect herbivory primary by chemical defense through high concentrations of tannins and anthocyanins. Additionally, low / values in young red leaves indicate that anthocyanins might not be functioning as light attenuators to compensate for insufficient photo-protection mediated by . And finally, red coloration in young leaves is predominantly a result of adaptation to heavy herbivory stress but without significant intrinsic phylogenetic relationship of plant species.

Ornaments can evolve to reveal individual quality when their production/maintenance costs make them reliable as ‘signals’ or if their expression level is intrinsically linked to condition by some unfalsifiable mechanism (indices). The latter has been mostly associated with traits constrained by body size. In red ketocarotenoid-based colorations, that link could, instead, be established with cell respiration at the inner mitochondrial membrane (IMM). The production mechanism could be independent of resource (yellow carotenoids) availability, thus discarding costs linked to allocation trade-offs. A gene coding for a ketolase enzyme (CYP2J19) responsible for converting dietary yellow carotenoids to red ketocarotenoids has recently been described. We treated male zebra finches with an antioxidant designed to penetrate the IMM (mitoTEMPO) and a thyroid hormone (triiodothyronine) with known hypermetabolic effects. Among hormone controls, MitoTEMPO downregulated CYP2J19 in the bill (a red ketocarotenoid-based ornament), supporting the mitochondrial involvement in ketolase function. Both treatments interacted when increasing hormone dosage, indicating that mitochondria and thyroid metabolisms could simultaneously regulate coloration. Moreover, CYP2J19 expression was positively correlated to redness but also to yellow carotenoid levels in the blood. However, treatment effects were not annulated when controlling for blood carotenoid variability, which suggests that costs linked to resource availability could be minor.

Background The control of plant anthocyanin accumulation is via transcriptional regulation of the genes encoding the biosynthetic enzymes. A key activator appears to be an R2R3 MYB transcription factor. In apple fruit, skin anthocyanin levels are controlled by a gene called MYBA or MYB1 , while the gene determining fruit flesh and foliage anthocyanin has been termed MYB10 . In order to further understand tissue-specific anthocyanin regulation we have isolated orthologous MYB genes from all the commercially important rosaceous species. Results We use gene specific primers to show that the three MYB activators of apple anthocyanin ( MYB10/MYB1/MYBA) are likely alleles of each other. MYB transcription factors, with high sequence identity to the apple gene were isolated from across the rosaceous family (e.g. apples, pears, plums, cherries, peaches, raspberries, rose, strawberry). Key identifying amino acid residues were found in both the DNA-binding and C-terminal domains of these MYBs. The expression of these MYB10 genes correlates with fruit and flower anthocyanin levels. Their function was tested in tobacco and strawberry. In tobacco, these MYBs were shown to induce the anthocyanin pathway when co-expressed with bHLHs, while over-expression of strawberry and apple genes in the crop of origin elevates anthocyanins. Conclusions This family-wide study of rosaceous R2R3 MYBs provides insight into the evolution of this plant trait. It has implications for the development of new coloured fruit and flowers, as well as aiding the understanding of temporal-spatial colour change.

Pyropia yezoensis , commonly known as “Nori” or “Laver” is an economically important marine crop. In natural or selected populations of P. yezoensis , coloration mutants are frequently observed. Various coloration mutants are excellent materials for genetic research and study photosynthesis. However, the candidate gene controlling the Pyropia coloration phenotype remains unclear to date. QTL-seq, in combination with kompetitive allele-specific PCR (KASP) and RNA-seq, can be generally applied to population genomics studies to rapidly identify genes that are responsible for phenotypes showing extremely opposite traits. Through cross experiments between the wild line RZ and red-mutant HT, offsprings with 1–4 sectors chimeric blade were generated. Statistical analyses revealed that the red thallus coloration phenotype is conferred by a single nuclear allele. Two-pair populations, consisting of 24 and 56 wild-type/red-type single-genotype sectors from F1 progeny, were used in QTL-seq to detect a genomic region in P. yezoensis harboring the red coloration locus. Based on a high-quality genome, we first identified the candidate region within a 3.30-Mb region at the end of chromosome 1. Linkage map-based QTL analysis was used to confirm the candidate region identified by QTL-seq. Then, four KASP markers developed in this region were used to narrow down the candidate region to a 1.42-Mb region. Finally, we conducted RNA-seq to focus on 13 differentially expressed genes and further predicted rcl-1 , which contains one non-synonymous SNP [A/C] in the coding region that could be regulating red thallus coloration in P. yezoensis . Our results provide novel insights into the underlying mechanism controlling blade coloration, which is a desirable trait in algae.

Cultivating red oak lettuce in plant factories often encounters challenges in achieving the desired red leaf coloration. To make the leaves a pleasant red color, anthocyanins are key substances that need to be induced. This study aimed to evaluate the effects of increasing light intensity and irrigation methods on the growth and leaf color of red oak lettuce in a controlled environment. Two light intensities (300 and 400 µmol m−2 s−1) with white LEDs and two irrigation methods (circulating vs. non-circulating irrigation) were applied seven days before harvesting. The results indicated that plants grown with circulating irrigation exhibited significantly higher fresh and dry weights than those grown under non-circulating conditions, regardless of light intensity. When non-circulating irrigation was applied, shoot fresh weight decreased by approximately 22% on the harvesting day compared to the circulating treatments. Under the 400 µmol m−2 s−1 light intensity with non-circulating irrigation (400N-C), plants displayed the lowest lightness (L*) at 40.7, increased redness (a*) to −7.4, and reduced yellowness (b*) to 11.0. These changes in coloration were optimized by day 5 after treatment. Additionally, spectral indices, including normalized difference vegetation index and photochemical reflectance index, varied significantly among treatments. The 400N-C treatment also resulted in the highest anthocyanin content and antioxidant activity in red oak lettuce. These findings suggest that combining high light intensity with non-circulating irrigation before harvest can improve both the coloration and quality of red oak lettuce in plant factories with artificial lighting.

Background Exaggerated signals, such as brilliant colours, are usually assumed to evolve through antagonistic coevolution between senders and receivers, but the underlying genetic mechanisms are rarely known. Here we explore a recently identified “redness gene”, CYP2J19 , that is highly interesting in this context since it encodes a carotenoid-modifying enzyme (a C4 ketolase involved in both colour signalling and colour discrimination in the red (long wavelength) spectral region.) Results A single full-length CYP2J19 was retrieved from 43 species out of 70 avian genomes examined, representing all major avian clades. In addition, CYP2J19 sequences from 13 species of weaverbirds (Ploceidae), seven of which have red C4-ketocarotenoid coloration were analysed. Despite the conserved retinal function and pleiotropy of CYP2J19 , analyses indicate that the gene has been positively selected throughout the radiation of birds, including sites within functional domains described in related CYP (cytochrome P450) loci. Analyses of eight further CYP loci across 25 species show that positive selection is common in this gene family in birds. There was no evidence for a change in selection pressure on CYP2J19 following co-option for red coloration in the weaverbirds. Conclusions The results presented here are consistent with an ancestral conserved function of CYP2J19 in the pigmentation of red retinal oil droplets used for colour vision, and its subsequent co-option for red integumentary coloration. The cause of positive selection on CYP2J19 is unclear, but may be partly related to compensatory mutations related to selection at the adjacent gene CYP2J40.

Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of flavonoid-metabolism proteins (6.5%). Following SSH, 566 clones were rescreened for differential gene expression using dot-blot hybridization. Of these, 53 were found to overexpressed during red coloration. The up-regulated expression of six genes was confirmed by Northern blot analyses. The expression of chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR) genes showed a positive correlation with anthocyanin accumulation in UV-B-irradiated lettuce leaves; flavonoid 3',5'-hydroxylase (F3',5'H) and anthocyanidin synthase (ANS) were expressed continuously in both samples. These results indicated that the genes CHS, F3H, and DFR coincided with increases in anthocyanin accumulation during the red coloration of lettuce leaves. This study show a relationship between red coloration and the expression of up-regulated genes in lettuce. The subtractive cDNA library and EST database described in this study represent a valuable resource for further research for secondary metabolism in the vegetable crops.

To determine how soil type, 2,4-dichlorophenoxyacetic acid (2,4-D) treatment, and storage affects color and anthocyanin accumulation of Red Norland potatoes, tubers were grown in sand or peat, with or without 2,4-D treatment, and measured at vine kill, harvest or after storage. Tubers grown in sand were less red and accumulated fewer anthocyanins than tubers grown in peat. 2,4-D treatment increased redness regardless of soil type. Redness loss varied greatly among tubers with storage. Tubers that lost color with storage had a two-fold reduction in anthocyanins, and a two-fold increase in benzoic and cinnamic acids compared to harvest, indicating chemical degradation of anthocyanidins via B-ring cleavage and autoxidation. Sand-grown potatoes did not exhibit greater cinnamic acids compared to peat-grown potatoes, suggesting that their color differences were due more to differences in biosynthesis than degradation during skin set. To improve Red Norland tuber color, research should focus on increasing biosynthesis of anthocyanins.

This work aimed to (i) determine the reliability of a portable Bluetooth colourimeter for fruit colour measurements; (ii) characterise the changes in quantitative skin colour attributes in a nectarine cultivar in response to time from harvest; and (iii) determine the influence of row orientation and training system on nectarine skin colour. The skin colour attributes measured with the colourimeter, namely L*, a* and b*, were calibrated and validated against a reference spectrophotometer. C* and h° were obtained from a* and b*. Skin colour was measured in situ from 42 days before to 6 days after harvest on ‘Majestic Pearl’ nectarines subjected to different row orientations and training systems. Validation models showed high reliability of colour estimations. The trends of colour attributes over time were characterised by cubic regression models, with h° proving to be the best parameter to describe changes of colour over time, with a clear link to the maturation process. No significant effects of row orientation and training system on skin colour were observed at harvest. Overall, the device proved reliable for fruit colour detection. Results of this study highlight the potential of h° as a quantitative index to monitor ripening prior to harvest in ‘Majestic Pearl’ nectarines.