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Brazil is the largest producer of sugarcane and the world’s top pesticide market. Therefore, environmental consequences are of concern. The aim of the present study was to evaluate the acute and chronic toxicity of pesticide formulations largely used in sugarcane crops: the herbicide DMA® 806 BR (a.i. 2,4-D) and the insecticide Regent® 800 WG (a.i. fipronil), isolated and in mixture, to the Neotropical cladoceran Ceriodaphnia silvestrii. Toxicity tests with the individual formulated products indicated 48h-EC50 values of 169 ± 18 mg a.i./L for 2,4-D and 3.9 ± 0.50 µg a.i./L for fipronil. In the chronic tests, the 8d-EC50 values for reproduction were 55 mg a.i./L (NOEC/LOEC: 50/60 mg a.i./L) and 1.6 µg a.i./L (NOEC/LOEC: 0.40/0.80 µg a.i./L) for 2,4-D and fipronil, respectively. A significant decrease in reproduction of C. silvestrii in all concentrations tested of fipronil, except at the lowest, was observed. Regarding 2,4-D, the organisms had total inhibition of reproduction in the two highest concentrations. Probably your energy reallocation was focused (trade-off) only on its survival. The acute pesticide mixture toxicity (immobility) revealed a dose level dependent deviation with antagonism at low and synergism at high concentrations. For chronic mixture (reproduction) toxicity, antagonism occurred as a result of the interaction of the pesticides. Based on our results and concentrations measured in Brazilian water bodies, fipronil represents ecological risks for causing direct toxic effects on C. silvestrii. These results are worrisome given that agricultural production is likely to increase in the coming years.

Wine is a rich source of biologically active compounds, particularly polyphenols, which exhibit antioxidant and antiradical properties. The objective of this study was to optimize the vinification procedures of Polish wines from the hybrid white grape cv. ‘Johanniter’ and red grape cv. ‘Regent’, grown in the temperate climate of central Europe, by applying different skin maceration times: 4, 8, 12, 16, and 20 days. The wines were compared for their basic oenological characteristics and polyphenolic (UHPLC–MS) content as well as their antioxidant (FRAP test) and antiradical (DPPH test, ORAC-fl and EPR spectroscopy) capacities. Both wines demonstrated a substantial increase in their total phenolic content and antioxidant and antiradical capacities after a 4-day maceration; further treatment did not lead to considerable enrichment in bioactive compounds. Scavenging activities against nitroxyl radicals and DPPH were divergent for the tested wines and depended on the analytical method applied, which indicated distinct molecular mechanisms. In turn, the activity of peroxyl radical scavengers, antioxidant capacity, and the total content of phenolics were higher in all the red wine samples. The antioxidant and antiradical properties of the examined wines were comparable or even exceeded those determined for most wines produced in regions with a rich winemaking tradition.

The study presents the potential use of winemaking residues, specifically grape pomace, for energy purposes. The pomace was obtained from the cultivation of the Regent grape variety on three different rootstocks—125AA, 161-49, and SO4—as well as a control group grown on its own roots. The research included determining the calorific value and combustion heat, conducting a technical and elemental analysis of the potential biofuel, as well as estimating emission indicators (CO, CO2, NOx, SO2, and particulate matter) and the theoretical volume of flue gases based on stoichiometric equations. The study revealed significant differences among the combustion heat, tested properties and calorific value, ash content, and the total volume of flue gases. The highest calorific value (17.7 MJ kg−1) and combustion heat (18.9 MJ kg−1) were obtained for pomace from the SO4SO4 rootstock, while the lowest values were observed in the control group (17.0 MJ·kg−1 and 15.8 MJ·kg−1, respectively). The highest ash content was also recorded for the SO4 rootstock (9.2%), with the lowest in the control group (6.7%). The control group exhibited the lowest CO2 emissions at 1390.50 kg·Mg−1, while the highest emissions were found in the pomace from the SO4 rootstock (1478.8 kg·Mg−1). Regarding the total flue gas volume, the highest volume was estimated for the pomace from the 125AA rootstock (7.8 m3·kg−1) and the lowest for the control group (7.3 m3·kg−1). The research demonstrated that grape pomace possesses favorable energy properties and could serve as a potential biofuel, contributing to the fuel and energy balance of agricultural production enterprises. The analyzed biomass exhibits properties similar to agrobiomass.

The aim of the study was to assess the biological potential of extracts obtained from the grape pomace of three hybrid varieties-Regent, Rondo and Marechal Foch-as a natural source of bioactive compounds, with possible application in cosmetology and dermatology. Grape pomace, which is an important by-product of the winemaking process, is a rich source of polyphenols, flavonoids, anthocyanins and vitamin C, which exhibit antioxidant, anti-inflammatory and cytoprotective properties that are important for skin health. The conducted studies determined the antioxidant activity of the extracts (DPPH) and the content of total phenolic compounds, flavonoids, anthocyanins and vitamin C, Of the varieties analysed: Marechal Foch exhibited the highest antioxidant activity (10 µmol TE/g), while Regent demonstrated the highest flavonoid content (50.42 mg/g) and vitamin C content (35.6 mg/100 g). The Rondo extract had the highest content of anthocyanins (362.36 mg/g) and total phenolic compounds (18.31 mg/g), indicating strong protective potential for skin cells. Regent extract at a concentration of 25 μg/mL was found to have the greatest effect on fibroblast proliferation and migration, significantly increasing the percentage of living cells and the rate of regeneration. This correlates with the high flavonoid content, which is particularly important for skin cells. These results confirm that grape pomace, particularly from the Regent variety, is a valuable source of natural antioxidants with anti-aging and regenerative properties. The use of these raw materials in cosmetic formulations aligns with the principles of the circular economy and the idea of \"zero waste\", being an example of the sustainable use of by-products from the wine industry in the production of innovative bio-cosmetics.

The regent honeyeater Anthochaera phrygia is a Critically Endangered Australian songbird, with current population estimates of < 300 individuals remaining in the wild. Low nest success is a factor preventing the recovery of the population, and management remedies are needed. However, a lack of data on intervention success raises uncertainty and impedes planning. To identify management priorities under uncertainty, we engaged with conservation practitioners and key stakeholders to develop and evaluate potential nest protection interventions. Four categories of threats were considered: avian predators, mammalian predators, extreme weather events and avian competitors. The interventions with the highest predicted probabilities of nest success under each threat category were, respectively: lethal control of avian predators, the use of tree collars to control arboreal mammalian predators, the provisioning of supplementary food and nesting resources during extreme weather events, and control of the noisy miner Manorina melanocephala, a competitor species. Our analysis shows that by applying a combination of conservation actions alongside improvements in nest detection, it is possible, based on the opinion of experts, to provide a pathway for the recovery of the regent honeyeater.

Although the court of Turin's role in the new balance of power in Europe during the War of the Spanish Succession is well known, far less is known about the strategic function of its collateral courts, such as the court of the princes of Savoy-Carignano. Based on the correspondence of the Savoy ambassador to Madrid, Costanzo uperti (1690-95), this article focuses on these courts to demonstrate the formal and informal diplomatic interplay among male and female aristocrats from 1640 to the end of the seventeenth century. une such noblewoman, ulimpia Mancini of Carignano-Soissons, was an Italian who grew up in the French court and maintained a close relationship with Louis XIV. As the wife of a prince of the Savoy-Carignano branch, she held important positions in Turin, Paris, and Madrid. Mother to the famous prince and military warrior Eugene of Savoy, after she lost her powerful status in France, she sought to find a place in the Madrid court as lady-in-waiting to Queen Marie Louise de urleans. Her mother-in-law, Marie de Bourbon-Soissons, played an outstanding role in maintaining the honour and prestige of the court of Carignano. Si le role de la cour de Turin dans le nouvel equilibre des puissances europeennes pendant la Guerre de Succession d'Espagne est bien connu, on en sait moins sur la fonction strategique d'autres cours, telle que celle des princes de Savoie-Carignan. S'appuyant sur la correspondance de l'ambassadeur de Savoie a Madrid Costanzo uperti (1690-1695), cet article s'interesse a ces cours, afin d'explorer le jeu diplomatique, formel et informel, auquel s'adonnerent les nobles - hommes et femmes - de 1640 a la fin du XVIIe siecle. Une de ces femmes nobles, ulympe Mancini de Carignan-Soissons, etait une Italienne elevee a la cour de France et proche du roi Louis XIV Epouse du prince Eugene Maurice de Savoie-Carignan, elle exerca une influence importante a Turin, Paris et Madrid. Mere du celebre prince et militaire Eugene de Savoie, elle obtint une place de dame d'honneur de la reine Marie-Louise d'urleans a la cour de Madrid, apres sa disgrace en France. Sa belle-mere, Marie de Bourbon-Soissons, joua un role de premier plan dans le maintien de l'honneur et du prestige de la cour de Savoie-Carignan.

Grapevine ( Vitis vinifera L.) is economically very important for the production of wine, table grapes and raisins. However, grapevine is threatened by a brought range of pathogens. A destructive disease worldwide is powdery mildew caused by the ascomycete Erysiphe necator. In the grapevine cultivar `Regent’ a resistance locus against E. necator , Ren3 , was previously reported. It spans an interval of approximately seven Mb on chromosome 15. We attempted to delimit this interval to facilitate its further molecular analysis. New simple sequence repeat markers targeted to the Ren3 region were designed. They were applied for fine mapping in the cross populations of ‘Regent’ × ‘Lemberger’ and ‘Regent’ × ‘Cabernet Sauvignon’ that segregate for E. necator resistance. Complementarily we scored E. necator infection levels of ‘Regent’ × ‘Lemberger’ progeny at different time points over the course of the vegetation period in 2015 and 2016. Subsequent QTL analysis revealed a maximum LOD value that shifted during the season from marker GF15-10 located at 2.2 Mb to marker GF15-53 located at 3.5 Mb and to marker ScORA7* located at 9.4 Mb on chromosome 15 (positions according to the grapevine reference genome of PN40024). To investigate the Ren3 -encoded resistance mechanism we performed detached leaf infection assays for microscopic studies. These revealed that Ren3 carrying individuals react with a hypersensitive response. Results of detached leaf assays on recombinants in the Ren3 locus indicate that not only one, but two distinct genetic regions on chromosome 15 mediate hypersensitive response against E. necator .

The aim of the study was the physicochemical characterization of wines produced using indigenous yeasts isolated from spontaneously fermented grape musts, obtained from cold climate grapes. Saccharomyces cerevisiae MH020215 and Nakawazaea ishiwadae MG971259 yeast strains were used in this study. The musts obtained from white and red grapes of Johanniter and regent varieties were used as a fermentation raw material. In the produced wines, content of ethyl alcohol, total extract, sugars, free amino nitrogen was analyzed, along with determination of total and volatile acidity and volatile compounds profile. Additionally, organoleptic evaluation was performed. Wines obtained with native S. cerevisiae MH020215 strains were characterized with more favorable enological properties. Synthesis of desirable volatile compounds, especially esters, contributed to the creation of desirable aromatic profile of those wines. Moreover, those beverages contained higher levels of carbonyl compounds (especially acetaldehyde) and lower methanol content. Wines obtained using N. ishiwadae MG971259 cultures were represented by high total acidity level and substantial fusel alcohol content (mainly butanol, propanol), which resulted in an unfavorable sensory profile of the product.