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This study aimed to investigate the protective effect of (L.) essential oil on rooster sperm motility during 4°C short-term storage. essential oil was analyzed using gas chromatography coupled to mass spectrometry to identify the active components. 10 of 45-week-old Hubbard commercial broilers were subjected to biweekly semen collections during 3 weeks. At each collection, sperm was pooled and divided into four aliquots and then diluted with Tris extender supplemented with 870, 87, or 8.7 µg/ml of essential oil, identified as treatments R, R5, and R10, respectively. Tris-based extender without any supplementation was considered as a control group. Diluted sperm was then stored at 4°C in the refrigerator and analyzed at 0, 6, 24, and 48 h using a computer-assisted sperm analyzer. Different semen parameters were measured including total motility, progressive motility, gametes velocities (straight line velocity [VSL], curvilinear velocity [VCL], and average path velocity [VAP]), amplitude of the lateral head displacement [ALH], and beat-cross frequency [BCF]. The phytochemical analysis of essential oil revealed the presence of 25 active components including seven major molecules: Camphor (18.88%), camphene (5.17%), 1,8-cineole (7.85%), β-thujene (13.66%), α-thujene (4.87%), chrysanthenone (12.05%), and β-cubenene (7.97%). The results showed a beneficial effect of essential oil on sperm cells motility, particularly when using the lowest concentrations, 8.7 and 87 µg/ml. Progressive motility and gametes velocities (VCL, VSL, and VAP), materializing the quality of gametes motility, showed highly statistically significant values (p<0.01) in 8.7 and 87 µg/ml treatments, especially from 6 h of storage at 4°C. Conversely, the highest concentration (870 µg/ml) showed harmful effects with a total spermicidal activity after 24 h of storage. The current results revealed the positive impact of essential oil on rooster sperm at 4°C short-term storage probably through fighting against oxidative stress and cold shock damages.

Functional food packaging films were prepared using a binary mixture of chitosan and gelatin through crosslinking with genipin and hybridization with rosemary essential oil and quercetin. The mixture of chitosan and gelatin produced the compatible film, and the added fillers also showed good compatibility. The physical properties of the chitosan/gelatin film were not greatly affected by crosslinking with genipin, and the functionality of the composite film was increased by the addition of rosemary essential oil and quercetin. The bioactive additives did not significantly affect the hydrophobicity and water vapor barrier properties of the chitosan/gelatin film but significantly changed the color, while the mechanical and thermal properties were slightly affected. The addition of these functional fillers significantly improved the UV protection, antioxidant, and antibacterial properties of the chitosan/gelatin film. Therefore, the novel chitosan/gelatin film with genipin crosslinking and the integration of rosemary essential oil and quercetin is considered to have high potential for applications in active food packaging.

Active films based on sodium caseinate (SC) were fabricated via the casting method; these films were reinforced with zinc oxide nanoparticles (ZnO NPs) and rosemary essential oil (REO). The films’ optical, mechanical, and barrier and antimicrobial properties were evaluated. Further characterization was done using SEM, XRD, and FTIR spectroscopy. The results disclosed that the incorporation of ZnO NPs and REO into the SC matrix significantly improved the mechanical and barrier properties as well as moisture resistance of the SC film. The simultaneous incorporation of ZnO NPs and REO considerably reduced the water vapor permeability (WVP) of the films but increased their flexibility and strength. The microscopic and spectroscopic characterization revealed that the additives were highly compatible with the SC film matrix given the formation of uniform and homogeneous composite films. The nanocomposite films also exhibited noteworthy antimicrobial activity against the tested bacteria. In summary, this type of biopolymer-based active film can be considered as a promising material for food packaging applications.

Rosemary (Rosmarinus officinalis L.) is a plant worldwide cultivated mainly for essential oils, extracts, and as a spice. Up-to-date results showed diversity in composition of the essential oils, which may influence their quality, biological activity, and thermal properties. Therefore, the aim of this study was to investigate the chemical composition, antimicrobial activity, and thermal properties of the rosemary essential oils originating from Serbia and Russia. Additionally, oils were added to the sunflower oils in order to investigate possible antioxidant activity during the frying. Investigation of the chemical profile marked α-pinene, eucalyptol, and camphor as the most abundant compounds in both oils. However, overall composition influenced in such manner that Russian oil showed significantly higher antimicrobial activity, while Serbian oil proved to be better antioxidant agent in case of frying of sunflower oil. This would significantly influence possible application of the oils, which could be used as an antioxidant agent for extension of the food shelf life, or antimicrobial agent for protection against different microbial strains.

The present study investigated the impact of edible coatings containing encapsulated antimicrobial and antibrowning agents on the quality and shelf life of fresh-cut potatoes. Polysaccharides extraction from potato peels using ultrasound/microwave-assisted extraction (UAE/MAE) was optimized via central composite design (CCD) and response surface methodology (RSM). Maximum starch recovery (216.55 ± 10.37 mg amylose/g potato peel) was achieved with UAE. Starch, pectin, and chitosan combination was the basis of the coatings. Rosemary essential oil (REO) and ascorbic acid (AA) were selected as antimicrobial and antioxidant agents, incorporated in free or encapsulated form via spray drying (SP) and electrospinning (EL). Over 14 days at 4 °C, coated potatoes exhibited improved appearance, reduced browning, enzyme activity, weight loss, hardness, and microbial load compared to controls. Notably, the potato starch-pectine coating with encapsulated REO/AA effectively inhibited browning and microbial growth, demonstrating the potential of edible coatings to enhance freshness and shelf life in fresh-cut potatoes. Graphical Abstract

The aim of this study is to develop three-layer chitosan films enriched with rosemary oil nanoemulsion (RONE) at concentrations of 0.5%, 1%, and 1.5%, to to enhance antimicrobial properties and control oil release. Gas chromatography–mass spectrometry (GC–MS) identified 1,8-Cineole (20.52%), borneol (19.6%), and α-pinene (17.57%) as the main components of rosemary essential oil (REO). RONE prepared via ultrasonic homogenization, had a particle size of 70.75 nm, a polydispersity index (PDI) of 0.299, and a zeta potential of − 8 mV. Films with RONE exhibited reduced hydrophobicity and water vapor permeability (WVP) but maintained tensile strength (TS) and elongation at break (EAB). Scanning electron microscopy (SEM) confirmed smooth and compact film structures. Films containing 1% and 1.5% RONE showed enhanced antimicrobial activity against Staphylococcus aureus and Bacillus subtilis . Minced beef stored at 4 °C for 12 days with these films demonstrated reduced growth of Pseudomonas spp. and H₂S-producing bacteria. Additionally, peroxide value (PV) and total volatile nitrogen (TVN) levels remained within acceptable limits. These results highlight the potential of RONE-enriched chitosan-based multilayer films as sustainable food packaging solutions with antimicrobial, antioxidant, and prolonged-release properties to extend shelf life.

This study was conducted to assess the impacts of using two essential oils, rosemary and ginger, on growing rabbits’ performance, carcass traits, meat composition, blood biochemicals, and the redox status of growing New Zealand White (NZW) rabbits. A total of 120 unsexed NZW rabbits, 42-days-old, were assigned randomly to five experimental groups (n = 24, 6 replicates with 4 rabbits each). The first group received a basal diet (control), the second to fifth groups were dietary supplemented daily with rosemary essential oil (REO) and ginger essential oil (GEO) at doses of 0.25 and 0.5% for each supplementation (REO-0.25, REO-0.5, GEO-0.25, and GEO-0.5), respectively. The growth traits were studied for 7 weeks, from the 7th to the 13th week of the rabbits’ age. The results revealed that final body weight, weight gain, and average daily gain increased significantly (p < 0.01) in the REO-0.5 and GEO-0.5 treatments compared to the control group. Daily feed intake decreased (p = 0.005) in essential oil treatments. Meanwhile, the feed conversion ratio improved significantly (p = 0.001) in REO and GEO at the high doses compared to the control group. The weight percentages of liver and giblets increased (p < 0.001) with both treatments of REO and GEO compared to the control group. The dietary supplementation with REO and GEO did not affect (p > 0.05) the meat composition of Longissimus dorsi and hind leg muscles. Meanwhile, REO and GEO supplementation significantly decreased cholesterol levels in the rabbit meat. Thiobarbituric acid reactive substance concentrations decreased by 10 and 15% in the meat of REO-0.5 and GEO-0.5 treatments, respectively, compared to the other groups. In the same trend, REO and GEO treatments induced a significant (p = 0.001) reduction in the plasma cholesterol concentrations and triglycerides compared to the control. The total antioxidant capacity increased by 7.60% and the malondialdehyde decreased by 11.64% in the plasma of GEO-0.5 treatment than the control. Thus, the dietary supplementation of REO and GEO have a beneficial effect in improving the productivity and meat quality of growing rabbits.

The foremost objective of this work is to assess the microcapsules composition (polymer-based and polymer/clay-based) effect, on the release of rosemary essential oil into w/o medium and evaluate their antioxidant activity. Calcium alginate (CA) and calcium alginate/montmorillonite hybrid (CA-MTN) microcapsules were developed following an ionotropic crosslinking gelation and were used as host materials for the encapsulation of rosemary essential oil. The unloaded/loaded CA and hybrid CA-MTN microcapsules were characterized by Fourier transform infra-red (FT-ATR) spectroscopy, thermal analysis (TGA), scanning electron microscopy (SEM) and DPPH assay. The evaluation of the microcapsule’s physicochemical properties has shown that the clay filling with montmorillonite improved the microcapsule’s properties. The encapsulation efficiency improved significantly in hybrid CA-MTN microcapsules and exhibited higher values ranging from 81 for CA to 83% for hybrid CA-MTN and a loading capacity of 71 for CA and 73% for hybrid CA-MTN, owing to the large adsorption capacity of the sodic clay. Moreover, the hybrid CA-MTN microcapsules showed a time-extended release of rosemary essential oil compared to CA microcapsules. Finally, the DPPH assay displayed a higher reduction of free radicals in hybrid CA-MNT-REO (12.8%) than CA-REO (10%) loaded microcapsules. These results proved that the clay–alginate combination provides microcapsules with enhanced properties compared to the polymer-based microcapsules.

Salt-induced stress poses a significant barrier to agricultural productivity by impeding crop growth. Presently, environmentalists are dedicated to safeguarding food security by enhancing agricultural yields in challenging environments. Biostimulants play a crucial role in mitigating abiotic stresses in crop production, and among these, plant essential oils (EOs) stand out as organic substances with diverse biological effects on living organisms. Among the natural promoters of plant growth, Rosmarinus officinalis L. essential oil (RoEO) has gained considerable attention. Although the manifold effects of essential oils (EOs) on plant growth have been extensively demonstrated, their impact on salt stress tolerance in durum wheat seedlings remains unexplored. This investigation was undertaken to evaluate the biostimulatory capabilities of RoEO on the durum wheat cultivar “Mahmoudi.” The effects of three RoEO concentrations (1, 2.5, and 5 ppm) on seed germination, growth establishment, and the induction of salt resistance under salinity conditions (150 mM NaCl) were tested. At 5 ppm, RoEO enhanced seedlings’ tolerance to salinity by improving growth and reducing membrane deterioration and oxidative stress-induced damage. The expression profile analyses of seven stress-related genes (TdNHX1, TdSOS1, TdSOD, TdCAT, TdGA20-ox1, TdNRT2.1, and TdGS) using RT-qPCR showed enhancement of several important genes in durum wheat seedlings treated with 5 ppm RoEO, even under control conditions, which may be related to salt stress tolerance. The results indicate that the application of RoEO suggests a possible alternative strategy to increase salt tolerance in durum wheat seedlings towards better growth quality, thus increasing ROS scavenging and activation of antioxidant defense.

Although rosemary essential oil (EO) shows many biological activities, its topical benefits have not been clearly demonstrated. In this work, we assessed the effects on skin hydration and elasticity of rosemary EO after topical application via gel vehicles in human volunteers. To improve its topical efficacy, rosemary EO was loaded into lipid nanoparticles (NLCs) consisting of cetyl palmitate as a solid lipid, and non-ionic surfactants. Such NLCs were prepared using different ratios of EO/solid lipid and those containing EO 3% w/w and cetyl pamitate 7% w/w were selected for in vivo studies, showing the best technological properties (small particle size, low polydispersity index and good stability). Gels containing free EO or EO-loaded NLCs were applied on the hand skin surface of ten healthy volunteers twice a day for one week. Skin hydration and elasticity changes were recorded using the instrument Soft Plus. Gels containing EO-loaded NLCs showed a significant increase in skin hydration in comparison with gels containing free EO. Skin elasticity increased, as well, although to a lesser extent. The results of this study point out the usefulness of rosemary EO-loaded NLCs for the treatment of cutaneous alterations involving loss of skin hydration and elasticity.