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A sieve shaker or a sieving apparatus translates the mechanical action of separating coarser, solid particles into finer grains in sectors such as pharmaceuticals and materials engineering. Segregating particles according to their desired sizes enables a more comprehensive and precise analysis. The movement of the filtering process is determined by the vibrations generated by the sieve shaker. The sieve shaker requires calibration to ensure the accuracy of the measurement during the sieving process. However, the back-to-back method mentioned in ISO 16063-21 is challenging to apply directly to calibrate the sieve shaker due to the unique shape of its body component. Therefore, in this study, we adopt a point-symmetrical position to perform vibration measurements produced by the sieve shaker. The standard accelerometer B&K 8035 is used as a reference transducer to measure the amplitude of the sieve shaker. The measurement was conducted over a displacement range of 0.2 to 3.00 mm, with the highest measurement uncertainty of 2%. The correction values obtained through the calibration process can be used to improve the accuracy of the sieve shaker particle segregation process.

This article assesses how urban segregation and ethnic diversity in Stockholm have been shaped by spatial policy and migration trajectories over time. Much of the urban studies and planning literature defines segregation as a measure of residential mixing. In contrast, our research suggests that segregation could be understood as a lack of opportunities for interaction in public space. In the case of Stockholm, space syntax network analysis and the establishment of ethnicity as a statistical category suggest that despite the social infrastructure provided by the Swedish state, the city’s specific spatial configuration alongside its policies of housing allocation have resulted in severe constraints on the potential for co-presence between new immigrants and the native Swedish population. Spatial analysis suggests that the city’s public transport infrastructure is a contributory factor in maintaining separation between foreign-born and ethnic Swedes. Coupled with a high level of social deprivation amongst new immigrants, the result is a multi-dimensional spatial segregation process that persists amongst the second immigrant generation, reinforcing ethnic and socio-economic area-based housing segregation. We conclude that despite Sweden’s long-standing political vision of social integration, its capital is suffering from increasing ethnic spatial differentiation, which will most likely persist unless a greater consideration of spatial connectivity and an introduction of ethnic and racial equality data in policy and practice are brought to bear. 本文评估了斯德哥尔摩的城市隔离和种族多样性如何随着时间的推移而受到空间政策和迁移轨迹的影响。许多城市研究和规划文献将隔离定义为居住混合的衡量标准。相比之下，我们的研究表明，隔离可以被理解为缺乏公共空间互动的机会。就斯德哥尔摩而言，空间句法网络分析和种族作为统计类别的建立表明，尽管瑞典国家提供了社会基础设施，但该城市的特定空间配置以及住房分配政策导致了对新移民与瑞典本土人口共存的潜力的严重限制。空间分析表明，该市的公共交通基础设施是维持外国出生和瑞典族裔分离的一个促成因素。加上新移民的高度社会剥夺，结果是在第二代移民中持续存在多维空间隔离过程，加强了基于种族和社会经济地区的住房隔离。我们的结论是，尽管瑞典长期拥有社会融合的政治愿景，但其首都却面临着越来越多的种族空间分化，除非更多地考虑空间连通性，并在政策和实践中引入种族和种族平等数据，否则这种分化很可能会持续存在。

Humans are remarkably skilled at listening to one speaker out of an acoustic mixture of several speech sources. Two speakers are easily segregated, even without binaural cues, but the neural mechanisms underlying this ability are not well understood. One possibility is that early cortical processing performs a spectrotemporal decomposition of the acoustic mixture, allowing the attended speech to be reconstructed via optimally weighted recombinations that discount spectrotemporal regions where sources heavily overlap. Using human magnetoencephalography (MEG) responses to a 2-talker mixture, we show evidence for an alternative possibility, in which early, active segregation occurs even for strongly spectrotemporally overlapping regions. Early (approximately 70-millisecond) responses to nonoverlapping spectrotemporal features are seen for both talkers. When competing talkers’ spectrotemporal features mask each other, the individual representations persist, but they occur with an approximately 20-millisecond delay. This suggests that the auditory cortex recovers acoustic features that are masked in the mixture, even if they occurred in the ignored speech. The existence of such noise-robust cortical representations, of features present in attended as well as ignored speech, suggests an active cortical stream segregation process, which could explain a range of behavioral effects of ignored background speech.

Many membraneless organelles (MLOs) formed through phase separation play crucial roles in various cellular processes. Although these MLOs co-exist in cells, how they maintain their independence without coalescence or engulfment remains largely unknown. Here, we investigated the molecular mechanism by which paraspeckles with core–shell architecture scaffolded by NEAT1_2 long noncoding RNAs exist as distinct MLOs. We identified NEAT1 deletion mutants that assemble paraspeckles that are incorporated into nuclear speckles. Several paraspeckle proteins, including SFPQ, HNRNPF and BRG1, prevent this incorporation and thus contribute to the segregation of paraspeckles from nuclear speckles. Shell localization of these proteins in the paraspeckles, which is determined by NEAT1_2 long noncoding RNA domains, is required for this segregation process. Conversely, U2-related spliceosomal proteins are involved in internalizing the paraspeckles into nuclear speckles. This study shows that the paraspeckle shell composition dictates the independence of MLOs in the nucleus, providing insights into the importance of the shell in defining features and functions of MLOs. Takakuwa, Yamazaki et al. show that the long noncoding RNA NEAT1 domains specify a set of proteins that localize to the outer shell and distinguish nuclear speckles from paraspeckles to preserve their structural identity.

A crucial step in life processes is the transfer of accurate and correct genetic material to offspring. During the construction of autonomous artificial cells, a very important step is the inheritance of genetic information in divided artificial cells. The ParMRC system, as one of the most representative systems for DNA segregation in bacteria, can be purified and reconstituted into GUVs to form artificial cells. In this study, we demonstrate that the eGFP gene is segregated into two poles by a ParM filament with ParR as the intermediate linker to bind ParM and parC -eGFP DNA in artificial cells. After the ParM filament splits, the cells are externally induced to divide into two daughter cells that contain parC -eGFP DNA by osmotic pressure and laser irradiation. Using a PURE system, we translate eGFP DNA into enhanced green fluorescent proteins in daughter cells, and bacterial plasmid segregation and inheritance are successfully mimicked in artificial cells. Our results could lead to the construction of more sophisticated artificial cells that can reproduce with genetic information. The accurate inheritance of the genetic material in offsprings is crucial for life. Here, the authors reported an artificial cell capable of the dynamic mimicry of the plasmid segregation process as well as the gene inheritance after division.

NME3 is a member of the nucleoside diphosphate kinase (NDPK) family localized on the mitochondrial outer membrane (MOM). Here, we report a role of NME3 in hypoxia-induced mitophagy dependent on its active site phosphohistidine but not the NDPK function. Mice carrying a knock-in mutation in the Nme3 gene disrupting NME3 active site histidine phosphorylation are vulnerable to ischemia/reperfusion-induced infarction and develop abnormalities in cerebellar function. Our mechanistic analysis reveals that hypoxia-induced phosphatidic acid (PA) on mitochondria is essential for mitophagy and the interaction of DRP1 with NME3. The PA binding function of MOM-localized NME3 is required for hypoxia-induced mitophagy. Further investigation demonstrates that the interaction with active NME3 prevents DRP1 susceptibility to MUL1-mediated ubiquitination, thereby allowing a sufficient amount of active DRP1 to mediate mitophagy. Furthermore, MUL1 overexpression suppresses hypoxia-induced mitophagy, which is reversed by co-expression of ubiquitin-resistant DRP1 mutant or histidine phosphorylatable NME3. Thus, the site-specific interaction with active NME3 provides DRP1 a microenvironment for stabilization to proceed the segregation process in mitophagy. NME3 is a member of NDPK family. Here, Chen et. al., discover that histidine phosphorylatable NME3 is required for hypoxia-induced mitophagy via PA-dependent interaction with Drp1, which is protected from MUL1-mediated ubiquitination for mitophagy.

DNA segregation by bacterial ParAB S systems is mediated by transient tethering interactions between nucleoid-bound dimers of the ATPase ParA and centromere ( parS )-associated complexes of the clamp-forming CTPase ParB. The lifetime of these interactions is limited by the ParB-dependent activation of ParA ATPase activity. Here, we elucidate the functional interplay between ParA and ParB in the model bacterium Myxococcus xanthus . We demonstrate that the N-terminal ParA-binding motif of ParB associates with a conserved bipartite binding pocket at the ParA dimer interface, in a manner dependent on ParB clamp closure. Moreover, we show that ParB and non-specific DNA interact cooperatively with ParA and synergistically induce structural changes in its Walker A and Walker B motifs that correlate with the activation of ParA ATPase activity. These results advance our understanding of the mechanism underlying DNA transport by the ParAB S system and may help to unravel the mode of action of related cargo-positioning systems. ParABS systems partition chromosomal DNA and low-copy plasmids in bacteria. Here, the authors elucidate the mode of interaction between ParA and ParB and clarify how ParB stimulates the ATPase activity of ParA to drive the segregation process.

Linear and nonlinear barotropic vorticity model frameworks are constructed to understand the formation of the monsoon trough in boreal summer over the western North Pacific. The governing equation is written with respect to specified zonal background flows, and a wave perturbation is prescribed in the eastern boundary. Whereas a uniform background mean flow leads no scale contraction, a confluent background zonal flow causes the contraction of zonal wavelength. Under linear dynamics, the wave contraction leads to the development of smaller scale vorticity perturbations. As a result, there is no upscale cascade. Under nonlinear dynamics, cyclonic (anticyclonic) wave disturbances shift northward (southward) away from the central latitude due to the vorticity segregation process. The merging of small-scale cyclonic and anticyclonic perturbations finally leads to the generation of a pair of large-scale cyclonic and anti-cyclonic vorticity gyres, straddling across the central latitude. The large-scale cyclonic circulation due to nonlinear upscale cascade can be further strengthened through a positive convection-circulation feedback.

The management and treatment of Medical Waste (MW) are of great concern owing to its potential hazard to human health and the environment, particularly in developing countries. In Bhutan, although guidelines exist on the prevention and management of wastes, the implementation is still hampered by technological, economic, social difficulties and inadequate training of staff responsible for handling these waste. The study aimed at assessing the awareness and practice of medical waste management among health care providers and support staff at the National Referral Hospital and its compliance with the existing National guidelines and policies. An observational cross-sectional study was conducted from March to April 2019. Three research instruments were developed and used; (i) Demographic questionnaire, (ii) Awareness questions, and (iii) the Observational checklist. The data was coded and double entered into Epi data version 3.1 and SPSS version 18 was used for analysis. Descriptive statistics were used to present the findings of the study. The majority of the respondents were female (54.1%) with a mean age of 32.2 (±7.67) years, most of whom have not received any waste management related training/education (56.8%). About 74.4% are aware of medical waste management and 98.2% are aware on the importance of using proper personal protective equipment. Only 37.6% knew about the maximum time limit for medical waste to be kept in hospital premises is 48 hours. About 61.3% of the observed units/wards/departments correctly segregated the waste in accordance to the national guidelines. However, half of the Hospital wastes are not being correctly transported based on correct segregation process with 58% of waste not segregated into infectious and general wastes. The awareness and practice of medical waste management among healthcare workers is often limited with inadequate sensitization and lack of proper implementation of the existing National guidelines at the study site. Therefore, timely and effective monitoring is required with regular training for healthcare workers and support staff. Furthermore, strengthening the waste management system at National Referral Hospital would provide beneficial impact in enhancing safety measures of patients.

Any material that is undesirable or unusable is considered waste. Waste can be in any form (Liquid, solid or gas) but generally, waste is a solid form. There are various types of waste like paper, displeasing food, torn clothes, dried plants, kitchen waste, etc., Skin disease, diarrhea, tuberculosis, whooping cough, pneumonia etc.., are some other common diseases spread due to immoral waste management. Separating waste allows us to salvage more items, preventing their scraping in landfills. By reducing landfills disposal Segregating waste is important not only to reduce its impact on the environment but also to prevent health issues that can arise from the disposal of waste and toxins. Although the problem of waste segregation is a big challenge, there are several methods for automatic segregation, which eliminates the need for human hands. The proposed system utilizes Faster R-CNN algorithms to segregate waste into bio-degradable and non-biodegradable categories which, effectively eliminating the need for human involvement in the segregation process. This method employs artificial intelligence techniques to achieve waste segregation.