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Zoraptera represent one of the smallest and least-known insect orders. They live mainly in tropical and subtropical forests and have a cryptic lifestyle. To obtain a better understanding of the detailed antennal morphology and its potential use for taxonomic research in this group, for the first time we conducted a scanning electron microscopy study of the antennal sensilla of Zoraptera. We examined two species of Spiralizoros (Spiralizoridae) and a single species of Spermozoros (Zorotypidae). We identified 10 different sensilla structures belonging to five main types in Zoraptera. While Böhm sensilla, sensilla campaniformia, sensilla chaetica (subtypes C1–C2), sensilla trichodea, and sensilla basiconica (subtypes B1–B2) were present in all species, sensilla chaetica C3, sensilla basiconica B3 and sensilla styloconica were present only in Spermozoros . We discussed the possible functions of all observed sensilla based on their external morphology. Additionally, we preliminarily compared the variability of antennal sensilla between the families as well as two species of the same genus, and investigated the differences between both sexes as well as apterons and dealates of the same species. This study provides the first step toward future research on antennal morphology within Zoraptera and its significance for their systematics.

Diverse sensory organs, including mammalian taste buds and insect chemosensory sensilla, show a marked compartmentalization of receptor cells; however, the functional impact of this organization remains unclear. Here we show that compartmentalized Drosophila olfactory receptor neurons (ORNs) communicate with each other directly. The sustained response of one ORN is inhibited by the transient activation of a neighbouring ORN. Mechanistically, such lateral inhibition does not depend on synapses and is probably mediated by ephaptic coupling. Moreover, lateral inhibition in the periphery can modulate olfactory behaviour. Together, the results show that integration of olfactory information can occur via lateral interactions between ORNs. Inhibition of a sustained response by a transient response may provide a means of encoding salience. Finally, a CO 2 -sensitive ORN in the malaria mosquito Anopheles can also be inhibited by excitation of an adjacent ORN, suggesting a broad occurrence of lateral inhibition in insects and possible applications in insect control. Olfactory receptor neurons of fruitflies are shown to communicate with one another through ephaptic interactions with significant impact on olfactory behaviour; the results indicate that ephaptic effects may be more widespread than previously appreciated. Cross-talk between adjacent neurons It has been suggested that the electrical activity of one neuron can influence that of its unlinked neighbour, but this so-called ephaptic effect is widely assumed to be weak and has been relatively little studied. Here, John Carlson and colleagues demonstrate that olfactory receptor neurons (ORNs) of fruitflies communicate with one another through large ephaptic signals with significant impact on olfactory behaviour. The sustained response of one ORN is inhibited by the transient activation of its neighbour. The authors also demonstrate that a CO 2 -sensitive ORN in the malaria mosquito Anopheles can be inhibited by excitation of an adjacent ORN and that lateral inhibition of a CO 2 neuron in Drosophila can reduce a behavioural response to CO 2 . As CO 2 is a key cue used by mosquitoes to find human hosts, this work suggests a novel means of insect control. These findings suggest that ephaptic effects may be more widespread than previously appreciated, particularly among neurons housed in confined peripheral sensory compartments such as taste buds.

Geckos have the extraordinary ability to prevent their sticky feet from fouling while running on dusty walls and ceilings. Understanding gecko adhesion and self-cleaning mechanisms is essential for elucidating animal behaviours and rationally designing gecko-inspired devices. Here we report a unique self-cleaning mechanism possessed by the nano-pads of gecko spatulae. The difference between the velocity-dependent particle-wall adhesion and the velocity-independent spatula-particle dynamic response leads to a robust self-cleaning capability, allowing geckos to efficiently dislodge dirt during their locomotion. Emulating this natural design, we fabricate artificial spatulae and micromanipulators that show similar effects, and that provide a new way to manipulate micro-objects. By simply tuning the pull-off velocity, our gecko-inspired micromanipulators, made of synthetic microfibers with graphene-decorated micro-pads, can easily pick up, transport, and drop-off microparticles for precise assembling. This work should open the door to the development of novel self-cleaning adhesives, smart surfaces, microelectromechanical systems, biomedical devices, and more. A running gecko can adhere to any surfaces at wet or dry conditions, whilst it keeps its toe pads clean. Here, Xu et al . attribute this self-cleaning mechanism to the rate-independent adhesion response between dirt particles and the gecko’s foot and use synthetic microfibers to reproduce the effect.

The antennal sensilla play an important role in many behavioral activities of insects. The fungivorous beetle Triplax ainonia Lewis (Erotylidae) is an important pest which prefers to feed on Pleurotus mushrooms. In order to clarify the types, number, and distribution of the antennal sensilla of male and female T . ainonia , scanning electron microscopy was used. The results showed that there were five sensillum types on the antennae of adults male and female, including Böhm’s bristles (BB), sensilla chaetica (three subtypes: SC 1, SC 2, and SC 3), sensilla basiconica (three subtypes: SB 1, SB 2, and SB 3), sensilla trichodea (ST), and sensilla styloconica (SS). Among all the sensilla, the number of SB 2 was the most abundant in both sexes. We found that there was no sexually dimorphic in the sensillum types, but there were differences in the number, lengths, and diameters of some sensilla between males and females. Based on the information of the morphology and distribution of the sensilla, the potential functions of the antennal sensilla of T . ainonia adults were discussed. The results of this study provide a basis for further study on the behavioral ecology and electrophysiology of the fungivore beetles of the Erotylidae.

Odorant binding proteins (Obps) are remarkable in their number, diversity, and abundance, yet their role in olfactory coding remains unclear. They are widely believed to be required for transporting hydrophobic odorants through an aqueous lymph to odorant receptors. We construct a map of the Drosophila antenna, in which the abundant Obps are mapped to olfactory sensilla with defined functions. The results lay a foundation for an incisive analysis of Obp function. The map identifies a sensillum type that contains a single abundant Obp, Obp28a. Surprisingly, deletion of the sole abundant Obp in these sensilla does not reduce the magnitude of their olfactory responses. The results suggest that this Obp is not required for odorant transport and that this sensillum does not require an abundant Obp. The results further suggest a novel role for this Obp in buffering changes in the odor environment, perhaps providing a molecular form of gain control. Insects use their sense of smell to find mates, to find food and – in the case of insects that transmit diseases such as malaria and Zika – to find us. If we can understand how insect scent detection works at the molecular and cellular level, we may be able to devise new ways of manipulating the insects’ sense of smell and prevent them from finding us. Insects contain a family of proteins called odorant binding proteins that are intriguing in several ways. They are numerous (there are 52 kinds in the fruit fly Drosophila), they are diverse and some are made in remarkably large amounts in the antennae. Fine hair-like structures known as olfactory sensilla protrude from the surface of the antennae. Odorant binding proteins are widely believed to carry odorant molecules through the fluid inside the sensilla to olfactory neurons, which then send signals that trigger the insect’s response to the scent. Larter et al. have now mapped the most abundant odorant binding proteins to the various olfactory sensilla of Drosophila. This revealed that a type of sensillum known as ab8 contained only one abundant odorant binding protein, called Obp28a. Unexpectedly, Larter et al. found that ab8 sensilla that are deprived of this protein respond strongly to odorant molecules. This result suggests that Obp28a is not required to transport odorants to the neurons in ab8; indeed, it appears that these neurons do not require an abundant odorant binding protein in order to respond to a scent. Instead, Obp28a helps to moderate the effects of sudden changes in the level of an odorant in the environment, so that concentrated odors do not trigger too large a response from the olfactory neurons. The details of the role that Obp28a plays in olfactory sensilla remain to be investigated in future studies, and the map created by Larter et al. also lays a foundation for studying the roles of other odorant binding proteins. The discovery that Obp28a is not needed to transport odorant molecules also raises questions about how insects are able to detect smells. Many odorant molecules repel water, so how do these molecules travel through the fluid in the sensilla if odorant binding proteins are not needed to transport them?

Two sympatric species Helicoverpa armigera and Helicoverpa assulta use (Z)-11-hexadecenal and (Z)-9-hexadecenal as sex pheromone components in reverse ratio. They also share several other pheromone gland components (PGCs). We present a comparative study on the olfactory coding mechanism and behavioral effects of these additional PGCs in pheromone communication of the two species using single sensillum recording, in situ hybridization, calcium imaging, and wind tunnel. We classify antennal sensilla types A, B and C into A, B1, B2, C1, C2 and C3 based on the response profiles, and identify the glomeruli responsible for antagonist detection in both species. The abundance of these sensilla types when compared with the number of OSNs expressing each of six pheromone receptors suggests that HarmOR13 and HassOR13 are expressed in OSNs housed within A type sensilla, HarmOR14b within B and C type sensilla, while HassOR6 and HassOR16 within some of C type sensilla. We find that for H. armigera, (Z)-11-hexadecenol and (Z)-11-hexadecenyl acetate act as behavioral antagonists. For H. assulta, instead, (Z)-11-hexadecenyl acetate acts as an agonist, while (Z)-9-hexadecenol, (Z)-11-hexadecenol and (Z)-9-hexadecenyl acetate are antagonists. The results provide an overall picture of intra- and interspecific olfactory and behavioral responses to all PGCs in two sister species.

In adult Lepidoptera the labial palps are best known for their role in CO2 detection, but they can also bear sensilla chaetica which function is unknown. The number and distribution of sensilla chaetica in labial palps was studied using a bright field microscope. To determine if these sensilla have a gustatory function, we performed single sensillum electrophysiology recordings from palp and antennal sensilla of adult moths of Cydia pomonella (L.), Grapholita molesta (Busck) and Lobesia botrana (Denis and Shieffermüller). Each sensillum was stimulated with 3 doses of one of four test stimulus (sucrose, fructose, KCl and NaCl). Overall, responses (spikes/s−1) increased with dose, and were higher in the palps than in the antennae, and higher to sugars than to salts. With sugars the response increased with concentration in the palp but not in the antenna. With salts there was a drop in response at the intermediate concentration. The number and position of sensilla chaetica on labial palps was variable among individuals. Sensilla were located in the most exposed areas of the palp. Differences in sensilla distribution were detected between species. Such differences among species and between palps and antenna suggest that taste sensilla on the palps have an unforeseen role in adaptation.

The sense of smell enables insects to recognize olfactory signals crucial for survival and reproduction. In insects, odorant detection highly depends on the interplay of distinct proteins expressed by specialized olfactory sensory neurons (OSNs) and associated support cells which are housed together in chemosensory units, named sensilla, mainly located on the antenna. Besides odorant-binding proteins (OBPs) and olfactory receptors, so-called sensory neuron membrane proteins (SNMPs) are indicated to play a critical role in the detection of certain odorants. SNMPs are insect-specific membrane proteins initially identified in pheromone-sensitive OSNs of Lepidoptera and are indispensable for a proper detection of pheromones. In the last decades, genome and transcriptome analyses have revealed a wide distribution of SNMP-encoding genes in holometabolous and hemimetabolous insects, with a given species expressing multiple subtypes in distinct cells of the olfactory system. Besides SNMPs having a neuronal expression in subpopulations of OSNs, certain SNMP types were found expressed in OSN-associated support cells suggesting different decisive roles of SNMPs in the peripheral olfactory system. In this review, we will report the state of knowledge of neuronal and non-neuronal members of the SNMP family and discuss their possible functions in insect olfaction.

The antennal flagellum of the locust S. gregaria is an articulated structure bearing a spectrum of sensilla that responds to sensory stimuli. In this study, we focus on the basiconic-type bristles as a model for sensory system development in the antenna. At the end of embryogenesis, these bristles are found at fixed locations and then on only the most distal six articulations of the antenna. They are innervated by a dendrite from a sensory cell cluster in the underlying epithelium, with each cluster directing fused axons topographically to an antennal tract running to the brain. We employ confocal imaging and immunolabeling to (a) identify mitotically active sense organ precursors for sensory cell clusters in the most distal annuli of the early embryonic antenna; (b) observe the subsequent spatial appearance of their neuronal progeny; and (c) map the spatial and temporal organization of axon projections from such clusters into the antennal tracts. We show that early in embryogenesis, proliferative precursors are localized circumferentially within discrete epithelial domains of the flagellum. Progeny first appear distally at the antennal tip and then sequentially in a proximal direction so that sensory neuron populations are distributed in an age-dependent manner along the antenna. Autotracing reveals that axon fasciculation with a tract is also sequential and reflects the location and age of the cell cluster along the most distal annuli. Cell cluster location and bristle location are therefore represented topographically and temporally within the axon profile of the tract and its projection to the brain.

The praying mantis Creobroter nebulosa Zheng (Mantedea: Hymenopodidae) is an insect that has medicinal and esthetical importance, and being a natural enemy for many insects, the species is used as a biological control agent. In this publication, we used scanning electron microscopy (SEM) to study the fine morphology of antennae of males and females of this species. The antennae of both sexes are filiform and consist of three parts: scape, pedicel, and flagellum (differing in the number of segments). Based on the external morphology and the sensilla distribution, the antennal flagellum is could be divided into five regions. Seven sensilla types and eleven subtypes of sensilla were observed: grooved peg sensillum (Sgp), Bohm bristles (Bb), basiconic sensillum (Sb), trichoid sensillum (StI, StII), campaniform sensillum (Sca), chaetic sensillum (ScI, ScII, ScIII), and coeloconic sensillum (ScoI, ScoII). In Mantodea, the ScoII is observed for the first time, and it is located on the tip of the flagellum. The external structure and distribution of these sensilla are compared to those of other insects and possible functions of the antennal sensilla are discussed. The males and females of the mantis could be distinguished by the length of antennae and number of Sgp. Males have antennae about 1.5 times longer and have significantly larger number of Sgp compared to females. The sexual difference in distribution of the Sgp suggests that this type of sensilla may play a role in sex-pheromones detection in mantis.