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Colorectal cancer (CRC) is widely prevalent and represents a significant contributor to global cancer-related mortality. There remains a pressing demand for advancements in CRC treatment modalities. The E3 ubiquitin ligase is a critical enzyme involved in modulating protein expression levels via posttranslational ubiquitin-mediated proteolysis, and it is reportedly involved in the progression of various cancers, making it a target of recent interest in anticancer therapy. In the present study, using comprehensive expression analysis involving spatial transcriptomic analysis with single-cell RNA sequencing in clinical CRC datasets, the ubiquitin-associated protein Shank-associated RH domain interactor (SHARPIN) was identified, located on amplified chromosome 8q, which could promote CRC progression. SHARPIN was found to be upregulated in tumor cells, with elevated expression observed in tumor tissues. This heightened expression of SHARPIN was positively associated with lymphatic invasion and served as an independent predictor of a poor prognosis in patients with CRC. In vitro and in vivo analyses using SHARPIN-overexpressing or -knockout CRC cells revealed that SHARPIN overexpression upregulated MDM2, resulting in the downregulation of p53, while SHARPIN silencing or knockout downregulated MDM2, leading to p53 upregulation, which affects cell cycle progression, tumor cell apoptosis and tumor growth in CRC. Furthermore, SHARPIN was found to be overexpressed in several cancer types, exerting significant effects on survival outcomes. In conclusion, SHARPIN represents a newly identified novel gene with the potential to promote tumor growth following apoptosis inhibition and cell cycle progression in part by inhibiting p53 expression via MDM2 upregulation; therefore, SHARPIN represents a potential therapeutic target for CRC.

Tobacco black shank, a destructive soil-borne disease caused by Phytophthora nicotianae , severely impacts tobacco production. Selenium (Se) is a beneficial trace element known to enhance plant stress resistance. While previous studies indicated Se’s efficacy against black shank, its differential effects on tobacco varieties with contrasting innate resistance remain unexplored. Herein, we integrated physiological, transcriptomic, and metabolomic analyses to elucidate the mechanisms of Se-induced resistance in a resistant (K326) and a susceptible (Zhongyan 100) tobacco cultivar. Our results showed that foliar application of 8 mg/L Se significantly reduced disease incidence and enhanced antioxidant enzyme activities, membrane stability, and accumulation of protective compounds in both cultivars. Multi-omics analyses revealed that Se potently enhanced resistance in K326 by synergistically upregulating the phenylpropanoid biosynthesis pathway, promoting the synthesis of lignin precursors and phenolic acids, and maintaining purine metabolism to ensure energy supply. In contrast, the susceptible cultivar Zhongyan 100 showed limited metabolic capacity to translate Se-induced transcriptional changes into effective defence metabolite accumulation. These findings provide novel insights into the genotype-dependent mechanisms of Se-induced resistance and highlight the potential of precision Se application as a strategy to bolster defence in resistant cultivars against soil-borne diseases.

The black shank disease seriously affects the health of tobacco plants. Conventional control methods have limitations in terms of effectiveness or economic aspects and cause public health concerns. Thus, biological control methods have come into the field, and microorganisms play a key role in suppressing tobacco black shank disease. In this study, we examined the impact of soil microbial community on black shank disease basing on the structural difference of bacterial communities in rhizosphere soils. We used Illumina sequencing to compare the bacterial community diversity and structure in different rhizosphere soil samples in terms of healthy tobacco, tobacco showing typical black shank symptoms, and tobacco treated with the biocontrol agent, Bacillus velezensis S719. We found that Alphaproteobacteria in the biocontrol group, accounted for 27.2% of the ASVs, was the most abundant bacterial class among three groups. Heatmap and LEfSe analyses were done to determine the distinct bacterial genera in the three sample groups. For the healthy group, Pseudomonas was the most significant genus; for the diseased group, Stenotrophomonas exhibited the strongest enrichment trend, and Sphingomonas showed the highest linear discriminant analysis score, and was even more abundant than Bacillus; for the biocontrol group, Bacillus, and Gemmatimonas were the largely distributed genus. In addition, co-occurrence network analysis confirmed the abundance of taxa, and detected a recovery trend in the network topological parameters of the biocontrol group. Further functional prediction also provided a possible explanation for the bacterial community changes with related KEGG annotation terms. These findings will improve our knowledge of plant-microbe interactions and the application of biocontrol agents to improve plant fitness, and may contribute to the selection of biocontrol strains.

Continuous cropping challenges have gradually emerged as pivotal factors limiting the sustainable development of agricultural production. Allelopathicals are considered to be the primary obstacles. However, there is limited information on allelopathic accumulation across various continuous cropping years and its correlation with the associated challenges. Tobacco was subjected to varying planting durations: 1 year (CR), 5 years (CC5), 10 years (CC10), and 15 years (CC15). Our findings unveiled discernible disparities in tobacco growth patterns across diverse continuous cropping periods. Notably, the most pronounced challenges were observed in the CC5 category, characterized by yield reduction, tobacco black shank outbreaks, and a decline in beneficial flora. Conversely, CC15 exhibited a substantial reduction in challenges as the continuous cropping persisted with no significant differences when compared to CR. Within the tobacco rhizosphere, we identified 14 distinct allelopathic compounds, with 10 of these compounds displaying noteworthy variations among the four treatments. Redundancy analysis (RDA) revealed that eight allelopathic compounds exhibited autotoxic effects on tobacco growth, with MA, heptadecanoic acid, and VA ranking as the most potent inhibitors. Interaction network highlighted the pivotal roles of VA and EA in promoting pathogen proliferation and impeding the enrichment of 13 beneficial bacterial genera. Furthermore, a structural equation model elucidated that MA and EA primarily exert direct toxic effects on tobacco, whereas VA fosters pathogen proliferation, inhibits the enrichment of beneficial bacteria, and synergistically exacerbates the challenges associated with continuous cropping alongside EA. These findings suggested discernible disparities in tobacco growth patterns across the various continuous cropping periods. The most pronounced challenges were observed in CC5, whereas CC15 exhibited a substantial reduction in challenges as continuous cropping persisted. VA may play a pivotal role in this phenomenon by interacting with pathogens, beneficial bacterial genera, and EA.

This article aims to compare the performance of two types of chisel ploughs as well as study the influence of shank types on indicators, average fuel consumption, slip percentage, field efficiency, plough shank vibration, and noise in the tractor driver’s environment. The experiment included three factors: the first one was plough shank type (rigid or flexible), and the second factor was two driving speeds (4.5 and 7.7 km·h ), while the third factor was two tillage depths (10 and 20 cm). The greatest effect of tractor driving speed was observed during acceleration, resulting in increased slippage by 26.39%, plough shank vibration by 17.42 m·s , and noise around the driver by 94.61 dB, with a reduction of fuel consumption by 15.87 l·ha and field efficiency by 61.94%. The results showed that the plough with the flexible shank outperformed compared to the rigid shank by having less fuel consumption 19.56 l·ha and slip of 17.31%, as well as the best field efficiency of 70.06% with a unique shank vibration of 22.63 m·s and the lowest noise emission around the driver 89.77 dB. The high speed of the flexible shank has a positive effect on fuel consumption 14.20 l·ha and shank vibrations 27.81 m·s

Tobacco black shank (TBS) disease, caused by Phytophthora nicotianae ( P. nicotianae ), poses a severe threat to tobacco productivity, necessitating the identification of effective control methods. Sulfur dioxide (SO 2 ) has emerged as a signaling molecule involved in modulating plant stress responses, yet its role in inducing resistance to TBS in tobacco remains unclear. This study investigated the potential of enhancing TBS resistance through root irrigation with SO 2 derivatives and elucidates the underlying mechanisms. Our findings revealed that SO 2 derivative root irrigation significantly enhanced tobacco resistance to TBS. This was evidenced by reduced malondialdehyde levels, increased hydrogen peroxide accumulation, and elevated activities of antioxidant enzymes such as superoxide dismutase and peroxidase. Moreover, analyses of phytohormones—jasmonic acid (JA), salicylic acid, abscisic acid, and ethylene—indicated a notable increase in endogenous JA levels in SO 2 -pretreated plants. The application of the JA biosynthesis inhibitor diethyldithiocarbamate acid (DIECA) significantly decreased JA levels and attenuated the SO 2 -induced TBS resistance. Furthermore, transcription of several JA-responsive defense genes was significantly upregulated in SO 2 -pretreated plants during P. nicotianae infection. These results demonstrate that SO 2 application elevates endogenous JA levels, thereby activating the antioxidant defense system and enhancing TBS resistance in tobacco plants. This study advances our understanding of SO 2 -induced resistance mechanisms and offers an effective, economical, and environmentally friendly strategy for managing soil-borne fungal diseases in crop production.

Tobacco black shank (TBS), caused by Phytophthora nicotianae , is one of the most harmful diseases of tobacco. There are many studies have examined the mechanism underlying the induction of disease resistance by arbuscular mycorrhizal fungi (AMF) and β-aminobutyric acid (BABA) alone, but the synergistic effects of AMF and BABA on disease resistance have not yet been studied. This study examined the synergistic effects of BABA application and AMF inoculation on the immune response to TBS in tobacco. The results showed that spraying BABA on leaves could increase the colonization rate of AMF, the disease index of tobacco infected by P.nicotianae treated with AMF and BABA was lower than that of P.nicotianae alone. The control effect of AMF and BABA on tobacco infected by P.nicotianae was higher than that of AMF or BABA and P.nicotianae alone. Joint application of AMF and BABA significantly increased the content of N, P, and K in the leaves and roots, in the joint AMF and BABA treatment than in the sole P. nicotianae treatment. The dry weight of plants treated with AMF and BABA was 22.3% higher than that treated with P.nicotianae alone. In comparison to P. nicotianae alone, the combination treatment with AMF and BABA had increased Pn, Gs, Tr, and root activity, while P. nicotianae alone had reduced Ci, H 2 O 2 content, and MDA levels. SOD, POD, CAT, APX, and Ph activity and expression levels were increased under the combined treatment of AMF and BABA than in P.nicotianae alone. In comparison to the treatment of P.nicotianae alone, the combined use of AMF and BABA increased the accumulation of GSH, proline, total phenols, and flavonoids. Therefore, the joint application of AMF and BABA can enhance the TBS resistance of tobacco plants to a greater degree than the application of either AMF or BABA alone. In summary, the application of defense-related amino acids, combined with inoculation with AMF, significantly promoted immune responses in tobacco. Our findings provide new insights that will aid the development and use of green disease control agents.

Tobacco black shank induced by Phytophthora nicotianae causes significant yield losses in tobacco plants. MicroRNAs (miRNAs) play a pivotal role in plant biotic stress responses and have great potential in tobacco breeding for disease resistance. However, the roles of miRNAs in tobacco plants in response to P. nicotianae infection has not been well characterized. In this study, we found that Nta-miR6155, a miRNA specific to Solanaceae crops, was significantly induced in P. nicotianae infected tobacco. Some of predicted target genes of Nta-miR6155 were also observed to be involved in disease resistance. To further investigate the function of miR6155 in tobacco during P. nicotianae infection, Nta-miR6155 overexpression plants (miR6155-OE) were generated in the Honghua Dajinyuan tobacco variety (HD, the main cultivated tobacco variety in China). We found that the Nta-miR6155 overexpression enhanced the resistance in tobacco towards P. nicotianae infections. The level of reactive oxygen species (ROS) was significantly lower and antioxidant enzyme activities were significantly higher in miR6155-OE plants than those in control HD plants during P. nicotianae infection. In addition, we found that the accumulation of salicylic acid and the expression of salicylic acid biosynthesis and signal transduction-related genes is significantly higher in miR6155-OE plants in comparison to the control HD plants. Furthermore, we found that Nta-miR6155 cleaved target genes NtCIPK18 to modulate resistance towards P. nicotianae in tobacco plants. Additionally, phenotypic analysis of miR6155-OE plants showed that Nta-miR6155 could inhibit the growth of tobacco by suppressing nitrogen uptake and photosynthesis. In conclusion, our findings indicated that miR6155 plays a crucial role in the regulation of growth and resistance against P. nicotianae infections in tobacco plants.

Black shank disease caused by Phytophthora nicotianae is one of the most important diseases in tobacco worldwide and can result in a devastating loss in tobacco cultivation. Many efforts have been carried out to identify the chromosome segment from Nicotiana plumbaginifolia containing a resistance locus carrying a gene named Php ; however, the Php gene has not been cloned, and knowledge of the potential mechanism of the Php gene in the resistant lines is limited. To further characterize the resistance mechanism of the Php gene, we first used the resistant line “RBST” and the susceptible cultivar “Honghuadajinyuan” (HD) to obtain the near-isogenic line RBS89 containing the Php gene from RBST. RBS89 showed high resistance to black shank disease. Transcriptomic and iTRAQ analyses were applied to explore the potential defense mechanisms in RBS89 plants in comparison with HD plants with or without inoculation. Many differentially expressed genes (DEGs) and proteins were identified, and some pathogenesis-related (PR) proteins were extensively abundant in the RBS89 plants when compared with the HD plants in response to black shank disease. Importantly, overexpression of the PR gene NtPR-1B in HD plants improved the resistance of tobacco plants to black shank disease, indicating that NtPR-1B and Php genes might have similar roles in protecting tobacco from black shank disease. However, the relationship between NtPR-1B and Php genes requires further analysis. Therefore, our study provides valuable information for breeding tobacco cultivars with black shank disease resistance and sheds light on the defense mechanism of black shank disease in tobacco for enhancing Phytophthora resistance in other Solanaceae crops.

Key words Tobacco black shank; Prevention; Treatment; Field (ProQuest: ... denotes formulae omitted.) 1 Introduction Tobacco is classified within the genus Nicotiana, family Solanaceae, order Tubiflorae, class Dicotyledoneae, and division Angiospermae. The mild consequences include a reduction in yield, while severe cases may result in the cessation of harvest activities, thereby causing significant economic losses for tobacco farmers. [...]this situation incurs substantial economic losses. (2) Relative control efficiency = ( Disease index in the control group - Disease index in the treatment group ) / Disease index in the control ...