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Existing experimental studies on human balance have primarily focused on biomechanical responses of major lower-limb joints, while the role of hallux and lesser toes of human foot in response to external perturbations has not been fully explored. Although toe grip strength may significantly influence balance performance, a more physiological-relevant toe grip evaluation, has not yet been established. This study investigates the biomechanical grip strength of the hallux and lesser toes during perturbations by quantifying the shear interactions (i.e., horizontal traction forces) at the foot–ground interface. A robotic platform with an instrumented multi-axial force platform was employed to analyze the involvement of the hallux and lesser toes in maintaining standing balance due to random ground perturbations. Our results indicate that hallux and lesser toes demonstrated significant direction-specific shear responses, and the proportion of shear traction force in the hallux and lesser toes significantly increased, particularly for perturbations in the posterior half-plane (p < 0.05). A substantial increase in shear traction force underneath the lesser toes was observed during contralateral perturbation events (p < 0.05). Importantly, the lesser toes could generate substantial shear traction forces by enhancing griping following perturbation, a capability not shown in other foot regions. This study introduced a novel approach for precisely quantifying the grip functions of individual toes at in-vivo perturbing conditions. The information provided is envisaged to have important implications on improved interventions for posture and balance rehabilitation.

Mechanobiology relates cellular processes to mechanical signals, such as determining the effect of variations in matrix stiffness with cell tractions. Cell traction recorded via traction force microscopy (TFM) commonly takes place on materials such as polyacrylamide- and polyethylene glycol-based gels. Such experiments remain limited in physiological relevance because cells natively migrate within complex tissue microenvironments that are spatially heterogeneous and hierarchical. Yet, TFM requires determination of the matrix constitutive law (stress–strain relationship), which is not always readily available. In addition, the currently achievable displacement resolution limits the accuracy of TFM for relatively small cells. To overcome these limitations, and increase the physiological relevance of in vitro experimental design, we present a new approach and a set of associated biomechanical signatures that are based purely on measurements of the matrix’s displacements without requiring any knowledge of its constitutive laws. We show that our mean deformation metrics (MDM) approach can provide significant biophysical information without the need to explicitly determine cell tractions. In the process of demonstrating the use of our MDM approach, we succeeded in expanding the capability of our displacement measurement technique such that it can now measure the 3D deformations around relatively small cells (∼10 micrometers), such as neutrophils. Furthermore, we also report previously unseen deformation patterns generated by motile neutrophils in 3D collagen gels.

With the increasing requirements of agricultural machinery, the study of the contact relationship between the tire–soil interface and the improvement of traction efficiency has gradually become a main concern. In this study, the pattern on the agricultural tire was simplified into single-pitch pattern blocks. The pattern blocks were made of rubber material that was highly resistant to abrasion and bending. The experiment was carried out by pressing the three types of patterned block construction into the soil and the pure sliding under the soil. The simulation used the Coupled Eulerian–Lagrangian Method (CEL) to verify the experimental results. We found that the herringbone pattern block was subjected to the highest stress for the same depth of downward pressure. The horizontal force generated by the pure sliding was also the highest. The results showed that the numerically simulated and experimentally measured data exhibited similar trends and average values. In addition, the increase in the contact area between the tire and the soil reduced the compaction and sinking of the soil. The herringbone pattern structure not only had a large contact area but also produced the most significant shear force on the soil. Thus, it may generate greater traction in actual operations.

The goodness-of-fit analysis performed over the results provided by a model presented in a previous paper proved that the theoretical data were very well correlated with the experimental data with regard to the traction force (with Pearson coefficient r2 over 0.9); however, the model was less accurate in predicting traction efficiency, with r2 = 0.203. In order improve the model and obtain a better fit between the theoretical and experimental data (especially for the traction efficiency), the model was updated and modified by taking into account the geometry of the tire cross section, which was considered to be a deformable ellipse. Due to the deformable cross section, the minor axis of the tire–ground contact super ellipse decreased compared with the previous model (from 0.367 m to 0.222 m), while the major axis increased (from 0.530 m to 0.534 m). As a result, different data for the traction force and traction efficiency were obtained. The effect of the wheel travel reduction (wheel slip) over the tire–soil shear area was also investigated, and the hypothesis of a constant shear area (independent of wheel slip) provided the most accurate results. The goodness-of-fit analysis performed using the data predicted by the modified model showed that the Pearson coefficient increased significantly with regard to the traction efficiency (from 0.203 to 0.838), while it decreased by only 2.7% with regard to the data for the traction force, still preserving a high value (r2 = 0.896).

While cells within tissues generate and sense 3D states of strain, the current understanding of the mechanics of fibrous extracellular matrices (ECMs) stems mainly from uniaxial, biaxial, and shear tests. Here, we demonstrate that the multiaxial deformations of fiber networks in 3D cannot be inferred solely based on these tests. The interdependence of the three principal strains gives rise to anomalous ratios of biaxial to uniaxial stiffness between 8 and 9 and apparent Poisson’s ratios larger than 1. These observations are explained using a microstructural network model and a coarse-grained constitutive framework that predicts the network Poisson effect and stress–strain responses in uniaxial, biaxial, and triaxial modes of deformation as a function of the microstructural properties of the network, including fiber mechanics and pore size of the network. Using this theoretical approach, we found that accounting for the Poisson effect leads to a 100-fold increase in the perceived elastic stiffness of thin collagen samples in extension tests, reconciling the seemingly disparate measurements of the stiffness of collagen networks using different methods. We applied our framework to study the formation of fiber tracts induced by cellular forces. In vitro experiments with low-density networks showed that the anomalous Poisson effect facilitates higher densification of fibrous tracts, associated with the invasion of cancerous acinar cells. The approach developed here can be used to model the evolving mechanics of ECM during cancer invasion and fibrosis.

Adhesive contact with the effect of tangential force may have important implications in friction and wear performances of small-sized devices and joining technologies. In the present work, adhesive contact involving tangential loading but before gross slip between spherical objects has been simulated through molecular dynamics (MD) to reveal the interaction between adhesion and the applied forces. When only the normal force is present, the results on force–displacement relationship and interfacial traction have been presented to compare with the predictions of Johnson–Kendall–Roberts (JKR), Maugis–Dugdale (M–D) and the Double–Hertz (D–H) models with the purpose of evaluating their applicability. In the presence of additional tangential forces, their interaction with adhesion has been studied in depth through loading and unloading. Distribution of the shear traction at the interface which is different from that in the existent models has been obtained. Those altogether may help to develop reasonable continuum models for adhesive contact under inclined forces.

The ocean is extremely rich in mineral resources. To cope with the shortage of land mineral resources, countries are focusing on the development of deep-sea mineral mining technology. Owing to its superior traction performance, the deep-sea polymetallic nodule collector (DPNC) has become the preferred solution for ocean mining. This paper proposes a dynamic traction calculation model to address the shortcomings in the classical static traction calculation model with consideration of the dynamic variation rule of grouser–soil interaction in the DPNC process. Lab tests were conducted to formulate materials similar to deep-sea soil, and the corresponding shear stress–displacement models were established using the discrete element method (DEM) and Magic Formula to describe the “shear stress–displacement” relationship more accurately. Considering Kunlong 500, which is a Chinese DPNC, as an example, the periodicity and dynamics of the dynamic traction force were analyzed and compared with the numerical simulation results. The dynamic traction force was smaller than the static traction force and fluctuated significantly when considering the dynamic grousers–soil interaction. The magnitude and fluctuation of the dynamic traction force were influenced by the ratio of the grouser height to the spacing. In the DPNC design, the ratio of the grouser height to the spacing should be optimized according to the properties of the deep-sea subsoil to improve the traction performance and stability of the DPNC.

The ability of bacteria to colonize and grow on different surfaces is an essential process for biofilm development. Here, we report the use of synthetic hydrogels with tunable stiffness and porosity to assess physical effects of the substrate on biofilm development. Using time-lapse microscopy to track the growth of expanding Serratia marcescens colonies, we find that biofilm colony growth can increase with increasing substrate stiffness, unlike what is found on traditional agar substrates. Using traction force microscopy-based techniques, we find that biofilms exert transient stresses correlated over length scales much larger than a single bacterium, and that the magnitude of these forces also increases with increasing substrate stiffness. Our results are consistent with a model of biofilm development in which the interplay between osmotic pressure arising from the biofilm and the poroelastic response of the underlying substrate controls biofilm growth and morphology.

Based on our direct shear creep experiment and the direct shear rheological constitutive model, a semi-empirical time-dependent parameter of the shear strength is obtained by Mohr–Coulomb shear strength theory, and different time-dependent traction force calculations between deep-sea sediment and a tracked miner are conducted by the work-energy principle. The time-dependent traction force calculation under its influencing factors, including the time, track shoe number, and grounding pressure, are analyzed and proved to be valid by the traction force experiment of a single-track shoe. The results show that the time-dependent cohesion force obtained by a semi-empirical way can be easily used to deduce the time-dependent traction force models under the different grounding pressure distributions and applied into deep-sea engineering application conveniently; the verified traction force models by the traction force experiment of a single-track shoe illustrate that traction force under the decrement grounding pressure distribution is the worst among the four kinds of grounding pressure distributions and suggested for evaluating the most unfavorable traction force and calculating the trafficability and stability of the deep-sea tracked miner.

The heterodimeric GABA B receptor, composed of GB1 and GB2 subunits, is a metabotropic G protein-coupled receptor (GPCR) activated by the neurotransmitter GABA. GABA binds to the extracellular domain of GB1 to activate G proteins through GB2. Here we show that GABA B receptors can be activated by mechanical forces, such as traction force and shear stress, in a GABA-independent manner. This GABA-independent mechano-activation of GABA B receptor is mediated by a direct interaction between integrins and the extracellular domain of GB1, indicating that GABA B receptor and integrin form a mechano-transduction complex. Mechanistically, shear stress promotes the binding of integrin to GB1 and induces an allosteric re-arrangement of GABA B receptor transmembrane domains towards an active conformation, culminating in receptor activation. Furthermore, we demonstrate that shear stress-induced GABA B receptor activation plays a crucial role in astrocyte remodeling. These findings reveal a role of GABA B receptor in mechano-transduction, uncovering a ligand-independent activation mechanism for GPCRs. In this study, the heterodimeric GABA B receptor, a class C G protein-coupled receptor for the neurotransmitter GABA, is found to be allosterically activated by mechanical forces in a GABA independent manner through a direct interaction with integrin.