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"Soils Kuwait Classification."
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Kuwait soil taxonomy
\"This book provides guidelines to key soil taxa in the deserts of Kuwait and guidance to associated procedures for laboratory analyses of soils, leading to land use planning on informed decisions. Soils are essential to provide food, feed, and fiber in addition to multiple ecosystem services that sustain life on earth. To achieve the above services sustainably, it is essential to use soils rationally based on their potential for specific uses. This requires establishing national soil classification systems to assess soils locally and to provide guidance to other countries where similar soils may be occurring. Once soil classification is established, it becomes easier to adopt technologies established on similar soils and environmental conditions without conducting long-term and expensive experimental trial. The taxa are established based on soil's morphological, physical, chemical, and mineralogical properties and climatic factors. It offers opportunities to maintain future soil surveys and their correlation to the soils of Kuwait. The book is useful in other arid region countries where similar soil and environmental conditions are existing, such as Bahrain, Oman, Qatar, and Saudi Arabia. The book also has international relevance, as it was prepared by extracting definitions from USDA-NRCS keys to soil taxonomy, and sections related to soils of Kuwait are added in the book. The book is a unique and excellent addition to the international soil literature.\"--Page 4 of cover.
Book
Oil-Contaminated Soil Modeling and Remediation Monitoring in Arid Areas Using Remote Sensing
Oil contamination is a major source of pollution in the environment. It may take decades for oil-contaminated soils to be remedied. This study models oil-contaminated soils using one of the world’s greatest environmental disasters, the onshore oil spill in the desert of Kuwait in 1991. This work uses state-of-art remote sensing technologies and machine learning to investigate the oil spills during the first Gulf War. We were able to identify oil-contaminated and clear locations in Kuwait using unsupervised classification over pre- and post-oil spill data. The research area’s pre-war and post-war circumstances, in terms of oil spills, were discovered by developing spectral signatures with different wavelengths and several spectral indices utilized for oil-contamination detection. Following that, we use this data for sampling and training to model various oil-contaminated soil levels. In addition, we analyze two separate datasets and used three modeling methodologies, Random Tree (RT), Support Vector Machine (SVM) and Random Forest (RF). The results show that the suggested approach is effective in detecting oil-contaminated soil. As a result, the location and degree of contamination may be established. The results of this analysis can be a valid support to the studies of an appropriate remediation.
Journal Article
Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill
Kuwaiti habitats with two-decade history of oil pollution were surveyed for their inhabitant oil-utilizing bacterioflora. Seawater samples from six sites along the Kuwaiti coasts of the Arabian Gulf and desert soil samples collected from seven sites all over the country harbored oil-utilizing bacteria whose numbers made up 0.0001–0.01% of the total, direct, microscopic counts. The indigenous bacterioflora in various sites were affiliated to many species. This was true when counting was made on nitrogen-containing and nitrogen-free media. Seawater samples harbored species belonging predominantly to the Gammaproteobacteria and desert soil samples contained predominantly Actinobacteria. Bacterial species that grew on the nitrogen-free medium and that represented a considerable proportion of the total in all individual bacterial consortia were diazotrophic. They gave positive acetylene-reduction test and possessed the
nifH
genes in their genomes. Individual representative species could utilize a wide range of aliphatic and aromatic hydrocarbons, as sole sources of carbon and energy. Quantitative determination showed that the individual species consumed crude oil,
n
-octadecane and phenanthrene, in batch cultures. It was concluded that the indigenous microflora could be involved in bioremediation programs without bioaugmentation or nitrogen fertilization. Irrigation would be the most important practice in bioremediation of the polluted soil desert areas.
Journal Article
Indigenous soil bacteria with the combined potential for hydrocarbon consumption and heavy metal resistance
Introduction
Transconjugant bacteria with combined potential for hydrocarbon utilization and heavy metal resistance were suggested by earlier investigators for bioremediation of soils co-contaminated with hydrocarbons and heavy metals. The purpose of this study was to offer evidence that such microorganisms are already part of the indigenous soil microflora.
Methods
Microorganisms in pristine and oily soils were counted on nutrient agar and a mineral medium with oil as a sole carbon source, in the absence and presence of either sodium arsenate (As V), sodium arsenite (As III) or cadmium sulfate, and characterized via 16S rRNA gene sequencing. The hydrocarbon-consumption potential of individual strains in the presence and absence of heavy metal salts was measured.
Results
Pristine and oil-contaminated soil samples harbored indigenous bacteria with the combined potential for hydrocarbon utilization and As and Cd resistance in numbers up to 4 × 10
5
CFU g
−1
. Unicellular bacteria were affiliated to the following species arranged in decreasing order of predominance:
Bacillus subtilis
,
Corynebacterium pseudotuberculosis
,
Brevibacterium linens
,
Alcaligenes faecalis
,
Enterobacter aerogenes
, and
Chromobacterium orangum
. Filamentous forms were affiliated to
Nocardia corallina
,
Streptomyces flavovirens
,
Micromonospora chalcea
, and
Nocardia paraffinea
. All these isolates could grow on a wide range of pure aliphatic and aromatic hydrocarbons, as sole sources of carbon and energy, and could consume oil and pure hydrocarbons in batch cultures. Low As concentrations, and to a lesser extent Cd concentrations, enhanced the hydrocarbon-consumption potential by the individual isolates.
Conclusion
There is no need for molecularly designing microorganisms with the combined potential for hydrocarbon utilization and heavy metal resistance, because they are already a part of the indigenous soil microflora.
Journal Article
Moderately thermophilic, hydrocarbonoclastic bacterial communities in Kuwaiti desert soil: enhanced activity via Ca(2+) and dipicolinic acid amendment
Pristine and oil-contaminated desert soil samples from Kuwait harbored between 10 and 100 cells g(-1) of hydrocarbonoclastic bacteria capable of growth at 50 °C. Enrichment by incubation of moistened soils for 6 months at 50 °C raised those numbers to the magnitude of 10(3) cells g(-1). Most of these organisms were moderately thermophilic and belonged to the genus Bacillus; they grew at 40-50 °C better than at 30 °C. Species belonging to the genera Amycolatopsis, Chelativorans, Isoptericola, Nocardia, Aeribacillus, Aneurinibacillus, Brevibacillus, Geobacillus, Kocuria, Marinobacter and Paenibacillus were also found. This microbial diversity indicates a good potential for hydrocarbon removal in soil at high temperature. Analysis of the same desert soil samples by a culture-independent method (combined, DGGE and 16S rDNA sequencing) revealed dramatically different lists of microorganisms, many of which had been recorded as hydrocarbonoclastic. Many species were more frequent in the oil contaminated than in the pristine soil samples, which may reflect their hydrocarbonoclastic activity in situ. The growth and hydrocarbon consumption potential of all tested isolates were dramatically enhanced by amendment of the cultures with Ca(2+) (up to 2.5 M CaSO4). This enhanced effect was even amplified when in addition 8 % w/v dipicolinic acid was amended. These novel findings are useful in suggesting biotechnologies for waste hydrocarbon remediation at moderately high temperature.
Journal Article
Actinomucor elegans var. kuwaitiensis isolated from the wound of a diabetic patient
The new variety of Actinomucor elegans var. kuwaitiensis, isolated from an open necrotic wound of a diabetic patient is described. This strain differed from the two previously described varieties of A. elegans, A. elegans var. elegans and A. elegans var. meitauzae by nearly 1% or more sequence divergence within D1/D2 regions of 28S rRNA and the ITS region of rRNA genes. Like the other two varieties, no zygospores were observed, however, there was evidence suggesting intersexual diploidy, a feature not described previously in this species. Additionally, A. elegans var. kuwaitiensis was pathogenic to white mice causing 100% mortality within 5 days.
Journal Article
A study of the enzymatic profile of soil isolates of Nocardia asteroides
In this study, using the API-ZYM system, we have reported the enzyme profile of 42 soil strains and 2 clinical strains of Nocardia asteroides isolated locally. Of the 19 enzymes tested, only 7 were demonstrable in over 90% of the soil isolates. These included alkaline phosphatase, esterase lipase, leucine arylamidase, acid phosphatase, phosphohydrolase, alpha-glucosidase and beta-glucosidase. In addition, beta-galactosidase activity was demonstrated in all the strains by the O-nitrophenyl-beta-D-galactopyranoside (ONPG) test. The enzymes which were not demonstrable in > 95% of the strains included valine arylamidase, cystine arylamidase, trypsin, chymotrypsin, alpha-galactosidase, beta-glucoronidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase and alpha-fucosidase. With the exception of valine arylamidase, which was lacking in all but one isolate, the enzyme profiles of the soil isolates were comparable with the clinical isolates of N. asteroides reported in previous studies. The reasons for this difference in the two sets of isolates is not clear. The study reinforces the view that specific differences in the enzymatic profiles of Nocardia species could be used for their rapid identification. However, more extensive studies are needed to establish the reproducibility of this method. To the best of our knowledge, this is the first study of the enzymatic profile of soil isolates of N. asteroides originating from a single geographic region.
Journal Article