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This study investigated the clinical characteristics, treatments, and outcomes of envenomation involving cobra species in Thailand (Naja kaouthia, Naja siamensis, and Naja sumatrana). Data of patients who had been bitten by a cobra or inoculated via the eyes/skin in 2018–2021 were obtained from the Ramathibodi Poison Center. There were 1045 patients admitted during the 4-year study period (bite, n = 539; ocular/dermal inoculation, n = 506). Almost all patients with ocular/dermal inoculation had eye involvement and ocular injuries, but none had neurological effects. Most of the patients bitten by a cobra had local effects (69.0%) and neurological signs and symptoms (55.7%). The median interval between the bite and the onset of neurological symptoms was 1 h (range, 10 min to 24 h). Accordingly, patients should be observed closely in hospitals for at least 24 h after a bite. Intubation with ventilator support was required in 45.5% of patients and for a median duration of 1.1 days. Antivenom was administered in 63.5% of cases. There were nine deaths, most of which resulted from severe infection. Neurological effects and intubation were significantly more common after a monocled cobra bite than after a spitting cobra bite. The administration of antivenom with good supportive care, including the appropriate management of complications, especially wound infection, might decrease fatality.

BiVO4 as a promising semiconductor absorber is widely investigated as photoanode in photoelectrochemical water splitting. Herein, the rational design of 3D hierarchical ternary SnO2/TiO2/BiVO4 arrays is reported as photoanode for photoelectrochemical application, in which the SnO2 hierarchically hollow microspheres core/nanosheets shell arrays act as conductive skeletons, while the sandwiched TiO2 and surface BiVO4 are working as hole blocking layer and light absorber, respectively. Arising to the hierarchically ordered structure and synergistic effect between each component in the composite, the ternary SnO2/TiO2/BiVO4 photoanode enables high light harvesting efficiency as well as enhanced charge transport and separation efficiency, yielding a maximum photocurrent density of ≈5.03 mA cm−2 for sulfite oxidation and ≈3.1 mA cm−2 for water oxidation, respectively, measured at 1.23 V versus reversible hydrogen electrode under simulated air mass (AM) 1.5 solar light illumination. The results reveal that electrode design and interface engineering play important roles on the overall PEC performance. A novel 3D hierarchical ternary SnO2/TiO2/BiVO4 arrays photoanode is designed with excellent photoelectrochemical performance.

Envenoming by cobras (Naja spp.) often results in extensive local tissue necrosis when optimal treatment with antivenom is not available. This study investigated the cytotoxicity of venoms and purified cytotoxins from the Monocled Cobra (Naja kaouthia), Taiwan Cobra (Naja atra), and Equatorial Spitting Cobra (Naja sumatrana) in a mouse fibroblast cell line, followed by neutralization of the cytotoxicity by three regional antivenoms: the Thai Naja kaouthia monovalent antivenom (NkMAV), Vietnamese snake antivenom (SAV) and Taiwanese Neuro bivalent antivenom (NBAV). The cytotoxins of N. atra (NA-CTX) and N. sumatrana (NS-CTX) were identified as P-type cytotoxins, whereas that of N. kaouthia (NK-CTX) is S-type. All venoms and purified cytotoxins demonstrated varying concentration-dependent cytotoxicity in the following trend: highest for N. atra, followed by N. sumatrana and N. kaouthia. The antivenoms moderately neutralized the cytotoxicity of N. kaouthia venom but were weak against N. atra and N. sumatrana venom cytotoxicity. The neutralization potencies of the antivenoms against the cytotoxins were varied and generally low across NA-CTX, NS-CTX, and NK-CTX, possibly attributed to limited antigenicity of CTXs and/or different formulation of antivenom products. The study underscores the need for antivenom improvement and/or new therapies in treating local tissue toxicity caused by cobra envenomings.

Captive belugas ( Delphinapterus leucas ) often spit water as object play. One female beluga spit water at a ball to obtain it for play. We examined whether or not this behavior could be considered a tool-use behavior. When the ball was placed 10 cm from the poolside, the beluga poked the ball with her rostrum, while she often spit water at the ball at 50cm. This water-spitting behavior became more predominantly when the ball was placed at 30 cm or farther, corresponding to the maximum distance she could reach, suggesting her understanding of the cause-effect relationship. Next, the two balls were placed at 30 cm and 60 cm, and one was placed inside the rings, so it would not be easy to move it out by water-spitting. When the closer ball was placed in the ring, she tried to spit water at the ball farther away but without the ring, suggesting an understanding of efficiency in tool use. Furthermore, during this study, the beluga showed the behavior of throwing the ball obtained by spitting water at another one with her mouth. This generalization may be based on the causal understanding established through water-spitting.

A facile electron-charging and reducing method was developed to prepare Au/＇VVO3 nanocomposites for plasmonic solar water splitting. The preparation method involved a charging step in which electrons were charged into WO3 under negative bias, and a subsequent reducing step in which the stored electrons were used to reductively deposit Au on the surface of WO3. The electron-charged WO3 （c-WO3） exhibited tunable reducibility that could be easily controlled by varying the charging parameters, and this property makes this method a universal strategy to prepare metalAVO3 composites. The obtained Au/VVO3 nanocomposite showed greatly improved photoactivity toward the oxygen evolution reaction （OER） when compared with WO3. After Au decoration, the OER photocurrent was improved by a percentage of over 80% at low potentials （〈0.6 V vs. SCE）, and by a percentage of over 30% at high potentials （〉1.0 V vs. SCE）. Oxygen evolution measurements were performed to quantitatively determine the Faraday efficiency for OER, which reflected the amount of photocurrent consumed by water splitting. The Faraday efficiency for OER was improved from 74% at the WO3 photoanode to 94% at the Au-8/＇vVO3 composite photoanode, and this is the first direct evidence that the Au decoration significantly restrained the anodic side reactions and enhanced the photoelectrochemical （PEC） OER efficiency. The high photoactivity of the composite photoanode toward OER was ascribed to the plasmon resonance energy transfer （PRET） enhancement and the catalytic enhancement of Au nanoparticles （NPs）.

The Samar Cobra, Naja samarensis , is endemic to the southern Philippines and is a WHO-listed Category 1 venomous snake species of medical importance. Envenomation caused by N. samarensis results in neurotoxicity, while there is no species-specific antivenom available for its treatment. The composition and neutralization of N. samarensis venom remain largely unknown to date. This study thus aimed to investigate the venom proteome of N. samarensis for a comprehensive profiling of the venom composition, and to examine the immunorecognition as well as neutralization of its toxins by a hetero-specific antivenom. Applying C 18 reverse-phase high-performance liquid chromatography (RP-HPLC) and tandem mass spectrometry (LC-MS/MS), three-finger toxins (3FTx) were shown to dominate the venom proteome by 90.48% of total venom proteins. Other proteins in the venom comprised snake venom metalloproteinases, phospholipases A 2, cysteine-rich secretory proteins, venom nerve growth factors, L-amino acid oxidases and vespryn, which were present at much lower abundances. Among all, short-chain alpha-neurotoxins (SαNTX) were the most highly expressed toxin within 3FTx family, constituting 65.87% of the total venom proteins. The SαNTX is the sole neurotoxic component of the venom and has an intravenous median lethal dose (LD 50 ) of 0.18 μg/g in mice. The high abundance and low LD 50 support the potent lethal activity of N. samarensis venom. The hetero-specific antivenom, Philippine Cobra Antivenom (PCAV, raised against Naja philippinensis ) were immunoreactive toward the venom and its protein fractions, including the principal SαNTX. In efficacy study, PCAV was able to cross-neutralize the lethality of SαNTX albeit the effect was weak with a low potency of 0.20 mg/ml (defined as the amount of toxin completely neutralized per milliliter of the antivenom). With a volume of 5 ml, each vial of PCAV may cross-neutralize approximately 1 mg of the toxin in vivo . The findings support the potential para-specific use of PCAV in treating envenomation caused by N. samarensis while underscoring the need to improve the potency of its neutralization activity, especially against the highly lethal alpha-neurotoxins.

Little is known about the detailed clinical description, pathophysiology, and efficacy of treatments for ocular envenoming (venom ophthalmia) caused by venom of the spitting elapid and other snakes, as well as ocular complications caused by snake venom injection. In this paper, we review clinical information of case reports regarding venom ophthalmia and snake venom injection with associated ocular injuries in Asia, Africa, and the United States. We also review the literature of snake venom such as their compositions, properties, and toxic effects. Based on the available clinical information and animal studies, we further discuss possible mechanisms of venom ophthalmia derived from two different routes (Duvernoy’s gland in the mouth and nuchal gland in the dorsal neck) and the pathophysiology of snake venom injection induced ocular complications, including corneal edema, corneal erosion, cataract, ocular inflammation, retinal hemorrhage, acute angle closure glaucoma, as well as ptosis, diplopia, and photophobia. Finally, we discuss the appropriate first aid and novel strategies for treating venom ophthalmia and snake envenoming.

•Spitting is common in police use of force events, occurring in 3.6% of the time.•Spitting may present new illness risk to officers given the COVID pandemic.•Identification of encounters at high risk for spitting may allow for attempts at mitigation. Spitting is an occupational hazard of police work with increased risk due to the COVID-19 pandemic. We sought to calculate the prevalence of spitting on police officers in use of force incidents, as well as the demographic and situational factors associated with spitting. Data on spitting were compiled from more than 10,000 use of force incidents occurring at 81 agencies in eight different states in the US. Spitting occurred in 3.6% of cases. Female and younger subjects and those using drugs and/or alcohol are more likely to spit on officers. Spitting is more likely to occur in incidents of longer duration, when officers use less force relative to subject resistance, when subjects are assaultive or engage in self-harm, and when subjects are hobbled. Spitting on officers is common and may now constitute a significant work hazard. Implications for police practice are discussed.

Phospholipase A2 (PLA2) toxins are one of the main toxin families found in snake venom. PLA2 toxins are associated with various detrimental effects, including neurotoxicity, myotoxicity, hemostatic disturbances, nephrotoxicity, edema, and inflammation. Although Naja sumatrana venom contains substantial quantities of PLA2 components, there is limited information on the function and activities of PLA2 toxins from the venom. In this study, a secretory PLA2 from the venom of Malaysian N. sumatrana, subsequently named A2-EPTX-Nsm1a, was isolated, purified, and characterized. A2-EPTX-Nsm1a was purified using a mass spectrometry-guided approach and multiple chromatography steps. Based on LC-MSMS, A2-EPTX-Nsm1a was found to show high sequence similarity with PLA2 from venoms of other Naja species. The PLA2 activity of A2-EPTX-Nsm1 was inhibited by 4-BPB and EDTA. A2-EPTX-Nsm1a was significantly less cytotoxic in a neuroblastoma cell line (SH-SY5Y) compared to crude venom and did not show a concentration-dependent cytotoxic activity. To our knowledge, this is the first study that characterizes and investigates the cytotoxicity of an Asp49 PLA2 isolated from Malaysian N. sumatrana venom in a human neuroblastoma cell line.

This study examined news media reports published during the early months of the COVID‐19 pandemic that described people spitting, coughing, and otherwise spreading respiratory fluids to other people and objects. A search of a news archive yielded more than 800 news articles published during March, April, and May, 2020, from which N = 325 cases of intentional pathogen spreading were identified. This was during the early part of the pandemic when the world was still trying to reach an understanding of how to deal with the pandemic. Collected news articles showed that cases of intentional pathogen spreading were reported to have occurred in 14 countries on 5 continents on most days (78.3%) of the 3‐month period considered. In 43% of cases, perpetrators claimed to be infected with the SARS‐CoV‐2 virus. Frontline key workers, passersby, and retail workers were the most frequent targets. The findings suggest that more needs to be learned about intentional pathogen spreading behavior, with the goals of reducing its occurrence in future pandemics and protecting vulnerable targets.