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The Arcanum mission is a proposed L-class mother-daughter spacecraft configuration for the Neptunian system, the mass and volume of which have been maximised to highlight the wide-ranging science the next generation of launch vehicles will enable. The spacecraft is designed to address a long-neglected but high-value region of the outer Solar System, showing that current advances make such a mission more feasible than ever before. This paper adds to a series on Arcanum and specifically provides progress on the study of areas identified as critical weaknesses by the 2013–2022 decadal survey and areas relevant to the recently published Voyage 2050 recommendations to the European Space Agency (ESA).

Background In fungal plant pathogens, genome rearrangements followed by selection pressure for adaptive traits have facilitated the co-evolutionary arms race between hosts and their pathogens. Pyrenophora tritici-repentis (Ptr) has emerged recently as a foliar pathogen of wheat worldwide and its populations consist of isolates that vary in their ability to produce combinations of different necrotrophic effectors. These effectors play vital roles in disease development. Here, we sequenced the genomes of a global collection (40 isolates) of Ptr to gain insights into its gene content and genome rearrangements. Results A comparative genome analysis revealed an open pangenome, with an abundance of accessory genes (~ 57%) reflecting Ptr’s adaptability. A clear distinction between pathogenic and non-pathogenic genomes was observed in size, gene content, and phylogenetic relatedness. Chromosomal rearrangements and structural organization, specifically around effector coding genes, were detailed using long-read assemblies (PacBio RS II) generated in this work in addition to previously assembled genomes. We also discovered the involvement of large mobile elements associated with Ptr’s effectors: ToxA , the gene encoding for the necrosis effector, was found as a single copy within a 143-kb ‘Starship’ transposon (dubbed ‘Horizon’) with a clearly defined target site and target site duplications. ‘Horizon’ was located on different chromosomes in different isolates, indicating mobility, and the previously described ToxhAT transposon (responsible for horizontal transfer of ToxA ) was nested within this newly identified Starship. Additionally, ToxB , the gene encoding the chlorosis effector, was clustered as three copies on a 294-kb element, which is likely a different putative ‘Starship’ (dubbed ‘Icarus’) in a ToxB-producing isolate. ToxB and its putative transposon were missing from the ToxB non-coding reference isolate, but the homolog toxb and ‘Icarus’ were both present in a different non-coding isolate. This suggests that ToxB may have been mobile at some point during the evolution of the Ptr genome which is contradictory to the current assumption of ToxB vertical inheritance. Finally, the genome architecture of Ptr was defined as ‘one-compartment’ based on calculated gene distances and evolutionary rates. Conclusions These findings together reflect on the highly plastic nature of the Ptr genome which has likely helped to drive its worldwide adaptation and has illuminated the involvement of giant transposons in facilitating the evolution of virulence in Ptr.

On 18 November 2023, SpaceX launched the Starship, the tallest and the most powerful rocket ever built. The Super Heavy engine separated from the Starship spacecraft and exploded at 90 km of altitude, while the main core Starship continued to rise up to 149 km and exploded after ∼8 min of flight. In this work, we used data from ground‐based GNSS receivers and we analyzed total electron content (TEC) response to the Starship flight and the two explosions. For the first time, we observed large‐distance northward propagation of intensive 2,000 km V‐shaped ionospheric disturbances from the rocket trajectory. The observed perturbations, most likely, represent shock waves propagating with the cone angle of ∼14° on the North and ∼7° on the South against the flight track that corresponds to the Mach angle of the shock waves in the lower atmosphere. The Starship explosion also produced a non‐chemical depletion in the ionospheric TEC. Plain Language Summary On 18 November 2023, SpaceX launched the Starship, the tallest and the most powerful rocket ever built. About 2 min and 40 s after the liftoff, the Super Heavy engine separated from the Starship spacecraft and exploded at an altitude of 90 km. The main core Starship continued to rise to 149 km and exploded as well. The rocket launch and explosion produced an unexpected response in the ionosphere—the ionized part of the Earth's atmosphere. The Starship flew at a velocity, exceeding the local sound speed, and generated cone‐like atmospheric shock‐acoustic waves. Most unexpectedly, the observed disturbances represented long and intensive multi‐oscillation wave structures that propagated northward, which is unusual for disturbances driven by a rocket launch. The Starship explosion also generated a large‐amplitude total electron content depletion that could have been reinforced by the impact of the spacecraft's fuel exhaust in the lower atmosphere. This study appears to be the first‐time detection of a non‐chemical ionospheric hole produced by a man‐made explosion. Key Points The 18 November 2023 Starship flight and explosions generated large‐scale multi‐oscillation supersonic conic waves in the ionosphere The cone angle of the V‐shaped ionospheric disturbances corresponds to the Mach angle of shock waves propagating in the lower ionosphere The shock waves from the Starship explosion caused a depletion in total electron content (TEC)

New outbreaks of fungal diseases are an ongoing threat to global agriculture. One known mechanism generating novel diseases is the horizontal transfer of genes between fungal species. Yet we have little understanding of how such transfers are mediated. Here, we raise the possibility that Starships, a recently discovered superfamily of giant transposable elements, might be responsible. To support this hypothesis, we discuss three potential cases where Starships may have mediated disease outbreaks. These are ToxA in wheat pathogens, genes underlying Glomerella leaf spot on apple trees, and the defoliating gene cluster of Verticillium dahliae on cotton. In the Verticillium example, we provide strong evidence for a Starship‐mediated mechanism: disease‐promoting genes reside in closely related Starships across distantly related species. We aim to spark interest in Starships' roles in fungal pathogens and how this knowledge could inform disease management strategies. Starships are massive transposons that can transfer virulence genes between fungal species, contributing to new plant disease outbreaks. We raise the possibility that Starships, a recently discovered superfamily of giant transposable elements, might be responsible for the horizontal transfer of genes between fungal species. Created in BioRender. https://BioRender.com/9ov40ep.

Recombination suppression can evolve in sex or mating-type chromosomes, or in autosomal supergenes, with different haplotypes being maintained by balancing selection. In the invasive chestnut blight fungus Cryphonectria parasitica, a genomic region was suggested to lack recombination and to be partially physically linked to the mating-type (MAT) locus based on segregation analyses. Using hundreds of available C. parasitica genomes and generating new high-quality genome assemblies, we show that a ca. 1.2 Mb genomic region proximal to the mating-type locus lacks recombination, with the segregation of two highly differentiated haplotypes in balanced proportions in invasive populations. High-quality genome assemblies further revealed an inversion in one of the haplotypes in the invaded range. The two haplotypes were estimated to have diverged 1.5 million years ago, and each harboured specific genes, some of which likely belonging to Starships. These are large transposable elements, mobilized by tyrosine recombinases, able to move accessory genes, and involved in adaptation in multiple fungi. The MAT-proximal region carried genes upregulated under virus infection or vegetative incompatibility reaction. In the native range, the MAT-proximal region also appeared to have a different evolutionary history than the rest of the genome. In all continents, the MAT-Proximal region was enriched in nonsynonymous substitutions, in gene presence/absence polymorphism, in tyrosine recombinases and in transposable elements. This study thus sheds light on a case of a large nonrecombining region partially linked to a mating compatibility locus, likely maintained by balancing selection on differentiated haplotypes, possibly involved in adaptation in a devastating tree pathogen.

Gaeumannomyces tritici is responsible for take-all disease, one of the most important wheat root threats worldwide. High-quality annotated genome resources are sorely lacking for this pathogen, as well as for the closely related antagonist and potential wheat take-all biocontrol agent, G. hyphopodioides . As such, we know very little about the genetic basis of the interactions in this host–pathogen–antagonist system. Using PacBio HiFi sequencing technology we have generated nine near-complete assemblies, including two different virulence lineages for G. tritici and the first assemblies for G. hyphopodioides and G. avenae (oat take-all). Genomic signatures support the presence of two distinct virulence lineages in G. tritici (types A and B), with A strains potentially employing a mechanism to prevent gene copy-number expansions. The CAZyme repertoire was highly conserved across Gaeumannomyces , while candidate secreted effector proteins and biosynthetic gene clusters showed more variability and may distinguish pathogenic and non-pathogenic lineages. A transition from self-sterility (heterothallism) to self-fertility (homothallism) may also be a key innovation implicated in lifestyle. We did not find evidence for transposable element and effector gene compartmentalisation in the genus, however the presence of Starship giant transposable elements may contribute to genomic plasticity in the genus. Our results depict Gaeumannomyces as an ideal system to explore interactions within the rhizosphere, the nuances of intraspecific virulence, interspecific antagonism, and fungal lifestyle evolution. The foundational genomic resources provided here will enable the development of diagnostics and surveillance of understudied but agriculturally important fungal pathogens.

The work presented here expands our understanding of a novel group of mobile genetic elements called Starships that facilitate the horizontal exchange of numerous genes between fungal pathogens. Our analysis shows that Sanctuary and ToxTA are both active transposons within the Bipolaris sorokiniana genome. We also show that the smaller ToxTA transposon has been independently acquired by two different Starships , namely Sanctuary in B. sorokiniana and Horizon in Pyrenophora tritici-repentis and Parastagonospora nodorum . Outside of ToxTA, these two Starships share no sequence identity. The acquisition of ToxTA by two different mobile elements in three different fungal wheat pathogens demonstrates how horizontal transposon transfer is driving the evolution of virulence in these important wheat pathogens.

A high-precision online trajectory optimization method combining convex optimization and Radau pseudospectral method is presented for the large attitude flip vertical landing problem of a starship-like vehicle. During the landing process, the aerodynamic influence on the starship-like vehicle is significant and non-negligible. A planar landing dynamics model with pitching motion is developed considering that there is no extensive lateral motion modulation during the whole flight. Combining the constraints of its powered descent landing process, a model of the fuel optimal trajectory optimization problem in the landing point coordinate system is given. The nonconvex properties of the trajectory optimization problem model are analyzed and discussed, and the advantages of fast solution and convergence certainty of convex optimization, and high discretization precision of the pseudospectral method, are fully utilized to transform the strongly nonconvex optimization problem into a series of finite-dimensional convex subproblems, which are solved quickly by the interior point method solver. Hardware-in-the-loop simulation experiments verify the effectiveness of the online trajectory optimization method. This method has the potential to be an online guidance method for the powered descent landing problem of starship-like vehicles.

The concept of a circular economy has become a significant school of thought in sustainable economics over the last 10 years. This paper critically analyses the key principles underpinning the concept of the circular economy, specifically examining the thermodynamic and ecological foundations upon which these principles are apparently rooted. We examine issues related to recycling, biological and technical nutrients, restoration, energy use, elimination of waste, eco-efficiency, product lifetime and economic growth under three headings: the pyramid of waste, short cycles and eco-inefficiency. We reflect on how the economy of nature is based on an open system, not a closed system, that nature operates using short cycles, not extended lifetimes, that nature is sub-optimal, not optimal and that nature is eco-inefficient, not eco-efficient. Findings are then discussed, and we explore what we can learn from the natural world in terms of sustainability.