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The present article applies Judith Butler’s notion of “grievable life” to reflect on the manner in which selected US-American television series engaged in the work of mourning and memorializing the loss of life in the first two years of the COVID-19 pandemic, with the aim of noting which lives were deemed “lose-able or injurable” (Butler, Frames 1), and how precarity of life was reflected by fictional narratives that were conceived and produced during the first waves of the pandemic. The article focuses in particular on the way in which network scripted programming operating within the melodramatic convention, namely This Is Us, Grey’s Anatomy and Station 19, incorporated pandemic storylines and which aspects of pandemic reality were highlighted or, conversely, avoided scrutiny.

Innovator Award for Edited Collection from the Central States Communication Association (CSCA)Shonda Rhimes is one of the most powerful players in contemporary American network television. Beginning with her break-out hit series Grey's Anatomy, she has successfully debuted Private Practice, Scandal, How to Get Away with Murder, The Catch, For The People, and Station 19. Rhimes's work is attentive to identity politics, \"post-\" identity politics, power, and representation, addressing innumerable societal issues. Rhimes intentionally addresses these issues with diverse characters and story lines that center, for example, on interracial friendships and relationships, LGBTIQ relationships and parenting, the impact of disability on familial and work dynamics, and complex representations of womanhood. This volume serves as a means to theorize Rhimes's contributions and influence by inspiring provocative conversations about television as a deeply politicized institution and exploring how Rhimes fits into the implications of twenty-first century television.  

Astroglia play active and diverse roles in modulating neuronal/synaptic functions in the CNS. How these astroglial functions are regulated, especially by neuronal signals, remains largely unknown. Exosomes, a major type of extracellular vesicles (EVs) that originate from endosomal intraluminal vesicles (ILVs), have emerged as a new intercellular communication process. By generating cell-type-specific ILVs/exosome reporter (CD63-GFP f/f ) mice and immuno-EM/confocal image analysis, we found that neuronal CD63-GFP + ILVs are primarily localized in soma and dendrites, but not in axonal terminals in vitro and in vivo. Secreted neuronal exosomes contain a subset of microRNAs (miRs) that is distinct from the miR profile of neurons. These miRs, especially the neuron-specific miR-124-3p, are potentially internalized into astrocytes. MiR-124-3p further up-regulates the predominant glutamate transporter GLT1 by suppressing GLT1-inhibiting miRs. Our findings suggest a previously undescribed neuronal exosomal miR-mediated genetic regulation of astrocyte functions, potentially opening a new frontier in understanding CNS intercellular communication. Our current understanding of exosome signaling among CNS cells is mostly limited to culture models. In this study, authors generated a new cell-type specific exosome reporter mouse line which allows the first in vivo investigation of the localization of neuronal exosomes in the CNS, and also potentially highlights the role of exosomally transferred miR-124-3p in mediating astroglial glutamate uptake function

Microgravity is associated with immunological dysfunction, though the mechanisms are poorly understood. Here, using single-cell analysis of human peripheral blood mononuclear cells (PBMCs) exposed to short term (25 hours) simulated microgravity, we characterize altered genes and pathways at basal and stimulated states with a Toll-like Receptor-7/8 agonist. We validate single-cell analysis by RNA sequencing and super-resolution microscopy, and against data from the Inspiration-4 (I4) mission, JAXA (Cell-Free Epigenome) mission, Twins study, and spleens from mice on the International Space Station. Overall, microgravity alters specific pathways for optimal immunity, including the cytoskeleton, interferon signaling, pyroptosis, temperature-shock, innate inflammation (e.g., Coronavirus pathogenesis pathway and IL-6 signaling), nuclear receptors, and sirtuin signaling. Microgravity directs monocyte inflammatory parameters, and impairs T cell and NK cell functionality. Using machine learning, we identify numerous compounds linking microgravity to immune cell transcription, and demonstrate that the flavonol, quercetin, can reverse most abnormal pathways. These results define immune cell alterations in microgravity, and provide opportunities for countermeasures to maintain normal immunity in space. The phenotype and function of immune cells could change during spaceflight. Here the authors use simulated microgravity, coupled to validation with spaceflight data, to assess whether there are distinct gene expression changes in resting and TLR 7/8 stimulated PBMCs and found conserved changes in IFN signalling, the cytoskeleton, IL-6 and sirtuin signalling.

Aotearoa New Zealand (Aotearoa), like many countries, experienced widespread demand for health services, threatening to collapse the health system. In addition to stringent border control, isolation policies for those with COVID-19, and instituting lockdowns, the government imposed a COVID-19 vaccine mandate for groups of essential workers, including healthcare professionals. Some literature argues that the COVID-19 vaccine mandates restrict individuals' freedoms through the loss of employment, income, and status as a healthcare professional. This qualitative research explored how COVID-19 vaccine mandates impacted healthcare professionals. Data from eight in-depth interviews with former healthcare professionals who experienced termination of their employment, and four managers or business owners were thematically analysed. The theme, Mandate-Induced Traumatic Decision-Making and Loss and two sub-themes, A Change in Attitudes and Ongoing Impacts on Lives, were identified. We found the COVID-19 vaccine mandates had detrimental impacts on those healthcare professionals affected by their decision not to have or complete COVID-19 vaccinations. Despite what participants believed were legitimate reasons for not being vaccinated, they experienced ongoing trauma and psychological, unemployment, and financial harm. The findings question the public good benefits of the vaccine mandate when it restricts the freedom, autonomy, and agency of much-needed healthcare professionals, which provide useful insights.

A vital question in neuroscience is how neurons align their postsynaptic structures with presynaptic release sites. Although synaptic adhesion proteins are known to contribute in this process, the role of neurotransmitters remains unclear. Here we inquire whether de novo biosynthesis and vesicular release of a noncanonical transmitter can facilitate the assembly of its corresponding postsynapses. We demonstrate that, in both stem cell-derived human neurons as well as in vivo mouse neurons of purely glutamatergic identity, ectopic expression of GABA-synthesis enzymes and vesicular transporters is sufficient to both produce GABA from ambient glutamate and transmit it from presynaptic terminals. This enables efficient accumulation and consistent activation of postsynaptic GABA A receptors, and generates fully functional GABAergic synapses that operate in parallel but independently of their glutamatergic counterparts. These findings suggest that presynaptic release of a neurotransmitter itself can signal the organization of relevant postsynaptic apparatus, which could be directly modified to reprogram the synapse identity of neurons. Neuronal communication relies on matching different neurostransmitter types with their appropriate receptors. The authors here demonstrate that release of a novel neurotransmitter from presynaptic terminals can induce both the accumulation and activation of its corresponding receptors on postsynaptic neurons.

To assess the impact of different non‐pharmaceutical interventions (NPIs) on COVID‐19 cases across Victoria and South Australia. Poisson regression models were fit to examine the effect of NPIs on weekly COVID‐19 case numbers. Mask‐wearing in Victoria had a pronounced lag effect of two weeks with an incidence rate ratio (IRR) of 0.27 (95%CI 0.26–0.29). Similarly, the effect of border closure (IRR 0.18; 95%CI 0.14–0.22) in South Australia and lockdown (IRR 0.88; 95%CI 0.86–0.91) in Victoria showed a decrease in incidence two weeks after the introduction of these interventions. With the ongoing COVID‐19 pandemic, varying levels of vaccination coverage rates and threats from variants of concern, NPIs are likely to remain in place. It is thus important to validate the effectiveness and timing of different interventions for disease control, as those that are more restrictive such as border control and lockdown can have an enormous impact on society. Low case numbers and deaths in Australia's first wave of COVID‐19 are thought to be due to the timely use of interventions. The observed two‐week lag effect associated with a decrease in incidence provides justification for early implementation of NPIs for COVID‐19 management and future pandemics.

This research applied secondary data collection methods to predict passenger flows, which involved analyzing traffic flows at rail stations and statistical methods, which involved assessing the relationship between variables and regression. Observation methods were used to measure current passenger flows at the rail station entrances. Passengers' safety perception was assessed to understand the customer value of transport services. This assessment was based on an online survey, an analysis of official requests from passengers to the station directorates, and feedback from passengers and station visitors posted on the Internet. Traditional and content analysis methods were used to study passengers' requests and feedbacks. It was substantiated that due to the COVID-19 pandemic, the concept of transportation security and the role of health safety as a customer value in passenger service have become more prominent. The possibility for passenger flow simulation to ensure people's safe passage through a disinfection gateway is clearly shown. Public space management structures in transportation hubs can use the simulation results of this paper to solve the problem of passenger flow control along with installation of disinfection gateways at the station entrances for additional security. This is one of the few studies that explores the impact of using disinfection gateways to manage passenger flows in train stations.

Health screening or examinations are of paramount importance from medical perspective as prevention is better than cure. In current pandemic situation, effective health screening is essential to detect COVID-19 in its early stage even when there have been no symptoms or signs of such disease. To prevent the transmission of such contagious infection, source control measures should be implemented for everyone in the facility, regardless of symptoms. Recent literatures stated that infected people having no symptoms also likely to perform a crucial role in the spread of corona virus in the society. To restrict the transmitting of such disease in community, it is imperative to identify the CORONA positive patient at each and every entry point of the market places, railway stations, airport premises, hospital buildings etc. and isolate them in an efficient manner. Rapid qualitative detection of such disease is made through body temperature measurement with IR thermal gun and RT-PCR test. Detection of COVID- 19 positive patient early means getting immediate treatment, restrict & control the transmission of such disease within the facility and limit the exposures for other patients and healthcare personnel.

Evidence indicates that astronauts experience significant bone loss in space. We previously showed that simulated microgravity (μXg) using the NASA developed rotary cell culture system (RCCS) enhanced bone resorbing osteoclast (OCL) differentiation. However, the mechanism by which μXg increases OCL formation is unclear. RANK/RANKL signaling pathway is critical for OCL differentiation. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) has been shown to increase osteoclastogenesis. We hypothesize that TRAIL may play an important role in μXg enhanced OCL differentiation. In this study, we identified by RT profiler PCR array screening that μXg induces high levels of TRAIL expression in murine preosteoclast cells in the absence of RANKL stimulation compared to ground based (Xg) cultures. We further identified that μXg elevated the adaptor protein TRAF-6 and fusion genes OC-STAMP and DC-STAMP expression in preosteoclast cells. Interestingly, neutralizing antibody against TRAIL significantly reduced μXg induced OCL formation. We further identified that over-expression of pTRAIL in RAW 264.7 cells enhanced OCL differentiation. These results indicate that TRAIL signaling plays an important role in the μXg increased OCL differentiation. Therefore, inhibition of TRAIL expression could be an effective countermeasure for μXg induced bone loss.