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• Ectomycorrhizal symbiosis is essential for the nutrition of most temperate forest trees and helps regulate the movement of carbon (C) and nitrogen (N) through forested ecosystems. The factors governing the exchange of plant C for fungal N, however, remain obscure. • Because competition and soil resources may influence ectomycorrhizal resource movement, we performed a 10-month split-root microcosm study using Pinus muricata seedlings with Thelephora terrestris, Suillus pungens, or no ectomycorrhizal fungus, under two N concentrations in artificial soil. Fungi competed directly with roots and indirectly with each other. We used stable isotope enrichment to track plant photosynthate and fungal N. • For T. terrestris, plants received N commensurate with the C given to their fungal partners. Thelephora terrestris was a superior mutualist under high-N conditions. For S. pungens, plant C and fungal N exchange were not coupled. However, in low-N conditions, plants preferentially allocated C to S. pungens rather than T. terrestris. • Our results suggest that ectomycorrhizal resource transfer depends on competitive and nutritional context. Plants can exchange C for fungal N, but coupling of these resources can depend on the fungal species and soil N. Understanding the diversity of fungal strategies, and how they change with environmental context, reveals mechanisms driving this important symbiosis.

This article provides information about the macrofungus Suillus granulatus (L) Roussel which was new one for South Uzbekistan. The identified macrofungus was found near the trees of Pinus eldarica Medw. The hymenium layer, basidiosporous basidia layer, spores and spores form small bodies (subfusiform) in the inner part of the spores in the shape of a drop.

Lead (Pb) is a highly toxic metal and a contaminant of many soils across the world. Some fungi are known to be Pb tolerant, persisting in environments with high Pb levels. Here we investigate Pb tolerance in Suillus brevipes and S. tomentosus , two widespread ectomycorrhizal fungal species in the American West where soil contamination due to mining is common. We conducted in vitro growth assays, exposing previously studied isolates to a range of Pb concentrations. We found S. tomentosus to be more Pb tolerant compared to S. brevipes and that isolates from the two species showed both high and low Pb tolerance. There were no correlations between Pb tolerance and Pb soil concentration, isolate growth rate, or Zn tolerance. Lead tolerance was positively correlated with Cd tolerance in S. tomentosus. Our research contributes for understanding fungal metal tolerance variability and paves the way for future work addressing the mechanisms of Pb tolerance and the potential for using Suillus in the recovery of contaminated sites.

Background Mutualistic mycorrhiza fungi that live in symbiosis with plants facilitates nutrient and water acquisition, improving tree growth and performance. In this study, we evaluated the potential of mutualistic fungal inoculation to improve the growth and disease resistance of Scots pine ( Pinus sylvestris L.) against the forest pathogen Heterobasidion annosum . Results In co-inoculation experiment, Scots pine seedlings were pre-inoculated with mutualistic beneficial fungus ( Suillus luteus ) prior to H. annosum infection. The result revealed that inoculation with beneficial fungus promoted plant root growth. Transcriptome analyses revealed that co-inoculated plants and plants inoculated with beneficial fungus shared some similarities in defense gene responses. However, pathogen infection alone had unique sets of genes encoding pathogenesis-related (PR) proteins, phenylpropanoid pathway/lignin biosynthesis, flavonoid biosynthesis, chalcone/stilbene biosynthesis, ethylene signaling pathway, JA signaling pathway, cell remodeling and growth, transporters, and fungal recognition. On the other hand, beneficial fungus inoculation repressed the expression of PR proteins, and other defense-related genes such as laccases, chalcone/stilbene synthases, terpene synthases, cytochrome P450s. The co-inoculated plants did not equally enhance the induction of PR genes, chalcone/stilbene biosynthesis, however genes related to cell wall growth, water and nutrient transporters, phenylpropanoid/lignin biosynthesis/flavonoid biosynthesis, and hormone signaling were induced. Conclusion S. luteus promoted mutualistic interaction by suppressing plant defense responses. Pre-inoculation of Scots pine seedlings with beneficial fungus S. luteus prior to pathogen challenge promoted primary root growth, as well as had a balancing buffering role in plant defense responses and cell growth at transcriptome level.

Boreal forest soils store a major proportion of the global terrestrial carbon (C) and below‐ground inputs contribute as much as above‐ground plant litter to the total C stored in the soil. A better understanding of the dynamics and drivers of root‐associated fungal communities is essential to predict long‐term soil C storage and climate feedbacks in northern ecosystems. We used 454‐pyrosequencing to identify fungal communities across fine‐scaled soil profiles in a 5000 yr fire‐driven boreal forest chronosequence, with the aim of pinpointing shifts in fungal community composition that may underlie variation in below‐ground C sequestration. In early successional‐stage forests, higher abundance of cord‐forming ectomycorrhizal fungi (such as Cortinarius and Suillus species) was linked to rapid turnover of mycelial biomass and necromass, efficient nitrogen (N) mobilization and low C sequestration. In late successional‐stage forests, cord formers declined, while ericoid mycorrhizal ascomycetes continued to dominate, potentially facilitating long‐term humus build‐up through production of melanized hyphae that resist decomposition. Our results suggest that cord‐forming ectomycorrhizal fungi and ericoid mycorrhizal fungi play opposing roles in below‐ground C storage. We postulate that, by affecting turnover and decomposition of fungal tissues, mycorrhizal fungal identity and growth form are critical determinants of C and N sequestration in boreal forests.

• While there has been significant progress characterizing the ‘symbiotic toolkit’ of ectomycorrhizal (ECM) fungi, how host specificity may be encoded into ECM fungal genomes remains poorly understood. • We conducted a comparative genomic analysis of ECM fungal host specialists and generalists, focusing on the specialist genus Suillus. Global analyses of genome dynamics across 46 species were assessed, along with targeted analyses of three classes of molecules previously identified as important determinants of host specificity: small secreted proteins (SSPs), secondary metabolites (SMs) and G-protein coupled receptors (GPCRs). • Relative to other ECM fungi, including other host specialists, Suillus had highly dynamic genomes including numerous rapidly evolving gene families and many domain expansions and contractions. Targeted analyses supported a role for SMs but not SSPs or GPCRs in Suillus host specificity. Phylogenomic-based ancestral state reconstruction identified Larix as the ancestral host of Suillus, with multiple independent switches between white and red pine hosts. • These results suggest that like other defining characteristics of the ECM lifestyle, host specificity is a dynamic process at the genome level. In the case of Suillus, both SMs and pathways involved in the deactivation of reactive oxygen species appear to be strongly associated with enhanced host specificity.

Belowground biota can deeply influence plant invasion. The presence of appropriate soil mutualists can act as a driver to enable plants to colonize new ranges. We reviewed the species of ectomycorrhizal fungi (EMF) that facilitate pine establishment in both native and non-native ranges, and that are associated with their invasion into nonforest settings. We found that one particular group of EMF, suilloid fungi, uniquely drive pine invasion in the absence of other EMF. Although the association with other EMF is variable, suilloid EMF are always associated with invasive pines, particularly at early invasion, when invasive trees are most vulnerable. We identified five main ecological traits of suilloid fungi that may explain their key role at pine invasions: their long-distance dispersal capacity, the establishment of positive biotic interactions with mammals, their capacity to generate a resistant spore bank, their rapid colonization of roots and their long-distance exploration type. These results suggest that the identity of mycorrhizal fungi and their ecological interactions, rather than simply the presence of compatible fungi, are key to the understanding of plant invasion processes and their success or failure. Particularly for pines, their specific association with suilloid fungi determines their invasion success in previously uninvaded ecosystems.

Background Bolete cultivation is economically and ecologically valuable. Ectomycorrhizae are advantageous for plant development and productivity. This study investigated how boletes affect the formation of Pinus thunbergii and Quercus acutissima ectomycorrhizae using greenhouse-based mycorrhizal experiments, inoculating P. thunbergii and Q. acutissima with four species of boletes ( Suillus bovinus , Suillus luteu s, Suillus grevillei , and Retiboletus sinensis ). Results Three months after inoculation, morphological and molecular analyses identified S. bovinus , S. luteu s, S. grevillei and R. sinensis ectomycorrhizae formation on the roots of both tree species. The mycorrhizal infection rate ranged from 40 to 55%. The host plant species determined the mycorrhiza morphology, which was independent of the bolete species. Differences in plant growth, photosynthesis, and endogenous hormone secretion primarily correlated with the host plant species. Infection with all four bolete species significantly promoted the host plants’ growth and photosynthesis rates; indole-3-acetic acid, zeatin, and gibberellic acid secretion increased, and the abscisic acid level significantly decreased. Indole-3-acetic acid was also detected in the fermentation broths of all bolete species. Conclusions Inoculation with bolete and subsequent mycorrhizae formation significantly altered the morphology and hormone content in the host seedlings, indicating growth promotion. These findings have practical implications for culturing pine and oak tree species.

This paper reports the results of the study of the efficiency of accumulation and distribution of mercury (Hg) in the fruiting bodies of fungus Suillus bovinus and the probable dietary intake of Hg and the potential health risk. Fungal fruiting bodies and soil materials were collected from 13 background areas in the northern part of Poland between 1993 and 2013. Mercury in the caps of fruiting bodies varied from 0.10 ± 0.06 to 0.79 ± 0.40 mg kg −1 dry biomass and in the stipes from 0.083 ± 0.028 to 0.51 ± 0.22 mg kg −1 dry biomass. The mean values of cap to stipe Hg content quotient varied from 1.3 ± 0.2 to 2.6 ± 0.6. The Hg content in the upper 0–10 cm layer of soil substrate varied from 0.015 ± 0.004 to 0.031 ± 0.019 mg kg −1 dry biomass. S. bovinus could be considered as an efficient accumulator of Hg, at least from low level polluted soils, and the values of Hg bioconcentration factor (BCF) varied from 6.4 ± 2.2 to 45 ± 20 for caps and from 3.8 ± 1.4 to 29 ± 11 for stipes. A conventional meal (300 g) portion of S. bovinus foraged from background areas provides Hg dose far below the provisionally tolerable weekly intake or recommended reference dose set for this element by authorities. An examination of published data on Hg in fruiting bodies of fungi genus Suillus showed low contamination of specimens foraged from background areas. Also reviewed are published data on Hg in fungi genus Suillus collected worldwide.

Masson pine is an important afforestation species in southern China, where seasonal drought is common. The present study focused on the effects of Suillus placidus, an ectomycorrhizal fungus, inoculation on the growth and physiological and biochemical performance of masson pine seedlings under four different watering treatments (well-watered, mild drought, moderate drought, and severe drought) to evaluate the symbiotic relationship between S. placidus and masson pine seedlings. Ectomycorrhizal-inoculated (ECM) and non-inoculated (NM) seedlings were grown in pots and maintained for 60 days using the weighing method. Results showed that seedlings’ growth, dry weight, RWC, chlorophyll content, PSII efficiency, and photosynthesis decreased as drought stress intensified in both ECM and NM plants. This suggests that drought stress significantly limits the growth and photosynthetic performance of masson pine seedlings. Nevertheless, increased An/gs and proline contents in both NM and ECM prevented oxidative damage caused by drought stress. In addition, increased peroxidase (POD) activity is an essential defense mechanism of ECM seedling under drought stress. Compared with NM, ECM seedlings showed faster growth, higher RWC, and photosynthetic performance, and lower lipid peroxidation in cell membranes under drought stress, as indicated by higher POD activity and lower proline and malondialdehyde (MDA). Our experiment found that S. placidus inoculation can enhance the drought resistance of masson pine seedlings by increasing antioxidant enzyme activity, water use efficiency, and proline content, thereby enhancing growth under water-deficiency conditions. S. placidus can be used to cultivate high-quality seedlings and improve their survival in regions that experience seasonal droughts.