Explore the vast range of titles available.

Résumé L’extraction de l’huile essentielle de Teucrium polium L. par hydrodistillation a permis d’obtenir un rendement moyen de 1,66 ± 0,12 %. Sa caractérisation par chromatographie en phase gazeuse couplée à la spectrométrie de masse montre une dominance des composés hydrocarbonés (77,85 %), avec 43,41 % de carbures monoterpéniques et 34,44 de carbures sesquiterpéniques. Les monoterpènes et les sesquiterpènes oxygénés révèlent un taux global de 20,31 %. L’activité antibactérienne montre une importante efficacité vis-à-vis des souches testées (75 % de sensibilité). Staphylococcus aureus enregistre le plus haut taux de sensibilité (100 %); Escherichia coli , Proteus mirabilis et Pseudomonas aeruginosa exhibent une résistance (33,33 %) vis-à-vis de l’huile essentielle. La concentration minimale inhibitrice est de 31,5 μl/ml, notée pour la souche Staphylococcus aureus . Cette inhibition est considérée comme très forte.

Teucrium polium L, known as felty germander or Palestinian Ja’adeh, primarily flourishes in Mediterranean countries such as Palestine, Jordan, and Turkey. There has been a significant increase in the investigation and analysis of essential oils derived from medicinal plants due to their potential biological and therapeutic applications. This study will investigate the chemical composition of essential oil derived from the dried leaves of T. polium L. and evaluate its in vitro antioxidant, anticancer, α -amylase, and lipase inhibitory properties. The essential oil extracted from the dried leaves of T. polium L. via hydrodistillation was analyzed using gas chromatography-mass spectrometry (GC-MS) to determine their chemical composition. Sixteen compounds were identified in the essential oil of T. polium L., with E-nerolidol (27.11%), geranyl acetone (23.26%), germacrene D (19.08%), β -caryophyllene (17.78%), α -caryophyllene (3.35%), and bicyclogermacrene (3.08%) constitute the principal components. This essential oil, abundant in oxygenated sesquiterpenes, demonstrated notable antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) at a concentration of 20.08 ± 1.00 µg/mL. Furthermore, it exhibited significant anticancer effectiveness against the MCF-7 and B16F10 cell lines, with IC 50 values recorded at 30.56 µg/mL and 32.75 µg/mL, respectively. Nevertheless, it demonstrated restricted lipase activity and insufficient α -amylase activity. The findings suggest that T. polium L. essential oil is a potential source of natural antioxidants and has anticancer properties, making it a viable candidate for pharmaceutical and cosmetic use.

Oxaliplatin (Oxa)-associated adverse side effects have considerably limited the clinical use of the drug in colon cancer therapy. Mutant p53 has diverse mutational profiles in colon cancer, and it influences the potencies of various chemotherapeutic drugs, including Oxa. Thus, it would be highly beneficial to identify an alternative therapeutic strategy that not only reduces the toxicity of Oxa, but also exerts a synergistic effect against colon cancers, regardless of their p53 profiles. The present study was aimed at preparing and optimizing Teucrium polium L. essential oil nanoemulsion (TPO-NANO) and investigating its effect on the sensitivity of colon cancer cells with differences in p53 status (HCT116 wild-type and HT-29 mutant-type) to Oxa. The viability of treated cells was determined and the combination index (CI) was calculated. Morphological changes were determined under inverted microscopy, while percentage apoptosis was assayed using flow cytometry. Intracellular ROS and the protein levels of p53 and Bax were measured. The colony-forming potential of treated cells was determined using colony assay. The size of TPO-NANO was markedly increased from 12.90 ± 0.04 nm to 14.47 ± 0.53 nm after loading Oxa (p ≤ 0.05). The combination (Oxa + TPO-NANO) produced a synergetic effect in HCT116 and HT-29, with CI of 0.94 and 0.88, respectively. Microscopic examination and flow cytometric analysis revealed that cells treated with Oxa + TPO-NANO had a higher percentage of apoptosis than cells exposed to monotherapy. Cumulatively, Oxa exerted an apoptotic effect on wild or mutant p53 colon cancer cells when combined with TPO-NANO, through a mechanism involving ROS-mediated mitochondrial apoptosis.

Teucrium polium L. is commonly used in folk medicine to treat hypertension and diabetes and to heal wounds. The present work aimed to evaluate the different biological activities of T. polium hydroalcoholic extract, its total phenol and flavonoid content, and its mineral elements. Results showed that T. polium extract showed significant antioxidant potential in 2-diphenyl-1-picrylhydrazyl (DPPH) assay with IC50 equal to 8.68 μg/mL but with moderate activity in galvinoxyl assay with IC50 of 21.82 μg/mL and mild activity in the β-carotene assay. It also showed a pronounced anti-hyperglycemic activity using α-amylase inhibitory assay (IC50 = 111.68 µg/mL) and exceeds that of acarbose. T. polium showed excellent activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with IC50 values of 28.69 and 4.93 μg/mL, respectively, postulating its promising anti-Alzheimer potential. The plant extract exhibited a strong anti-inflammatory effect with Bovine Serum Albumin (BSA) denaturation inhibitory potential estimated by 97.53% at 2 mg/mL, which was further confirmed by the in vivo carrageen-induced edema model. The extract revealed its richness in flavonoids and phenols, evidenced by its polyphenols content (36.35 ± 0.294 μg GAE/mg) and flavonoids (24.30 ± 0.44 μg QE/mg). It is rich in minerals necessary for human health, such as calcium, potassium, iron, sodium, magnesium, manganese and zinc. Molecular docking performed for previously identified compounds on human α-amylase, 5-lipoxygenase (5-LOX) and acetylcholine esterase confirmed the results. Thus, it can be concluded that T. polium can be a good candidate for alleviating many health-debilitating problems and can be highly beneficial in the pharmaceutical industry and medical research.

In this study, we investigate the phytochemical profile, anticancer, and antioxidant activities of Teucrium polium methanolic extract using both in vitro and in silico approaches. The results showed the identification of 29 phytochemical compounds belonging to 13 classes of compounds and 20 tripeptides using High Resolution-Liquid Chromatography Mass Spectrometry (HR-LCMS). 13R-hydroxy-9E,11Z octadecadienoic acid, dihydrosamidin, valtratum, and cepharantine were the main compounds identified. The tested extract showed promising antioxidant activities (ABTS-IC50 = 0.042 mg/mL; 1,1-diphenyl-2-picrylhydrazyl (DPPH)-IC50 = 0.087 mg/mL, β-carotene-IC50 = 0.101 mg/mL and FRAP-IC50 = 0.292 mg/mL). Using both malignant Walker 256/B and MatLyLu cell lines, T. polium methanolic extract showed a dose/time-dependent antitumor activity. The molecular docking approach revealed that most of the identified molecules were specifically binding with human peroxiredoxin 5, human androgen, and human progesterone receptors with high binding affinity scores. The obtained results confirmed that T. polium is a rich source of bioactive molecules with antioxidant and antitumor potential.

The chemical profile of Teucrium polium L. (T. polium) methanolic extract was tested using liquid chromatography coupled with high resolution mass spectrometry (HR-LCMS). Disc diffusion and microdilution assays were used for the antimicrobial activities. Coxsackievirus B-3 (CVB3) and Herpes simplex virus type 2 (HSV-2) were used for the antiviral activities. Chromobacterium violaceum (ATCC 12472 and CV026) and Pseudomonas aeruginosa PAO1 were used as starter strains for the anti-quorum sensing tests. Isoprenoids are the main class of compounds identified, and 13R-hydroxy-9E,11Z-octadecadienoic acid, valtratum, rhoifolin, sericetin diacetate, and dihydrosamidin were the dominant phytoconstituents. The highest mean diameter of growth inhibition zone was recorded for Acinetobacter baumannii (19.33 ± 1.15 mm). The minimal inhibitory concentrations were ranging from 6.25 to 25 mg/mL for bacterial strains, and from 6.25 to 25 mg/mL for Candida species. The 50% cytotoxic concentration on VERO (African Green Monkey Kidney) cell lines was estimated at 209 µg/mL. No antiviral activity was recorded. Additionally, T. polium extract was able to inhibit P. aeruginosa PAO1 motility in a concentration-dependent manner. However, the tested extract was able to inhibit 23.66% of the swarming and 35.25% of swimming capacities of PAO1 at 100 µg/mL. These results highlighted the role of germander as a potent antimicrobial agent that can interfere with the virulence factors controlled by the quorum-sensing systems.

The current work is devoted to using essential oils isolated from two wild-growing plants of Matricaria chamomilla and Teucrium polium in resistance to food spoilage, considering their antioxidant and antibacterial activities. Hydro-distillation extraction (HD) method was used to obtain essential oils and gas chromatography–flame ionization detection and gas chromatography–mass spectrometry were used to identify oils. Thus, the antioxidant activity of the oils was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS) radical scavenging method. In all, 45 constituents, such as 1,8-cineole, germac-rene-D, and artemisia ketone, were found in M chamomilla oil, contributing 89.06% of the total composition. T. polium L. oil contained 37 compounds, contributing 93.84% of the total composition; the two major compounds being beta-pinene at 74.81% and alpha-pinene at a proportion of 14.62%. The significant antioxidant and antibacterial properties of the above essential oils may affect the food sector to serve as natural and safe food preservatives. Thus, the study demonstrated that the identified essential oils of M. chamomilla L. and T. polium L. could be used as natural bioactive materials to form useful and prospective food products with enriched healthy properties.

Background/Objectives: Teucrium polium L. is widely used in traditional medicine and has been proposed as a source of antimicrobial adjuvants in the context of antimicrobial resistance. Here, we characterized the essential oil (EO) and polar extracts of T. polium and evaluated their antioxidant activity, antimicrobial potency against clinical multidrug-resistant (MDR) isolates, and the interaction of the EO with conventional antibiotics using a chequerboard assay (FICI); further, we investigated in silico molecular interactions with some targets related to resistance. Methods/Results: The EO, which was hydrodistilled and subsequently analyzed by GC–MS, is characterized by dominant limonene content (24.13%) and contents of oxygenated sesquiterpenes such as β-eudesmol (10.48%) and α-muurolol (8.10%). HPLC/UV–ESI–MS characterization of the extracts (decoction and Soxhlet) demonstrated that they were rich in polyphenolic compounds and flavonoids, which matched the standard phytochemical characteristics of this species. The extracts exhibited significant reducing capabilities, and the hydroethanolic extract exhibited the highest antioxidant activity (DPPH IC50 = 15.41 μg/mL; FRAP EC50 = 30.65 μg /mL), while the EO revealed at most moderate capacity in these tests. In antimicrobial assays, the EO inhibited fungi more effectively than the extracts (MIC of 1.17 mg/mL against Aspergillus niger; 4.69 mg/mL against Candida spp.), while antibacterial MICs for both the EO and extracts were generally high (up to 50 mg/mL). Combination testing nevertheless identified synergistic or additive effects of the EO with selected antibiotics, notably with ceftazidime against ESBL-producing Escherichia coli (FICI = 0.141) and Staphylococcus aureus (FICI = 0.039) and with amikacin against Klebsiella pneumoniae (FICI = 0.313); the EO–ceftriaxone pairing against ESBL E. coli was additive (FICI = 0.516). Docking simulations further supported these observations by showing the favorable predicted binding of oxygenated sesquiterpenes, most notably β-eudesmol and α-muurolol (up to −8.6 kcal/mol), to resistance-related targets such as RND efflux pumps, β-lactamases, and porins. Conclusions: Taken together, the in vitro and in silico data suggest that T. polium could be explored as a natural antimicrobial option and as an adjuvant to enhance antibiotic activity against multidrug-resistant pathogens.

Dental caries is a highly prevalent chronic condition globally. In recent years, scientists have turned to natural compounds such as plant extracts as an alternative to address concerns related to biofilm‐mediated disease transmission, increasing bacterial resistance, and the adverse impacts of antibiotics. Consequently, this study investigated the antimicrobial properties of ethanolic, hydroethanolic, and aqueous extracts of Teucrium polium L. (T. polium), which belongs to the Lamiaceae family, at different concentrations (0.1, 1, 10, 100, 500, 1000 ppm) against seven bacteria commonly associated with dental decay. The hydroethanolic extract demonstrated the highest efficacy against S. mutans (minimum inhibitory concentration (MIC) = 1.24 mg/mL), while the ethanolic extract exhibited the most potent activity against S. sanguinis (MIC = 1.55 mg/mL). For S. sobrinus, the ethanolic extract was the most effective (MIC = 1.52 mg/mL), whereas the hydromethanolic extract displayed the highest efficacy against S. salivarius (MIC = 1.52 mg/mL). S. aureus was most susceptible to the ethanolic extract (MIC = 1.9 mg/mL), whereas the aqueous extract demonstrated the strongest antimicrobial effect against S. epidermidis (MIC = 2.03 mg/mL). Finally, the ethanolic extract exhibited the maximum efficacy against L. fermentum (MIC = 1.36 mg/mL). Overall, the ethanolic extract demonstrated the highest efficacy against all tested bacteria, followed by the hydroethanolic extract, while the aqueous extract showed comparatively lower effectiveness. Therefore, depending on the specific target bacteria, it is suggested to combine the antibacterial extract of T. polium with the most effective solvent to effectively combat the bacteria responsible for dental decay. The study found that mouthwashes containing ethanolic and hydroethanolic extracts, at a concentration of 2.44 mg/L, effectively inhibited the growth of all oral bacteria contributing to dental caries. Future research should explore T. polium extracts' mechanisms of action against oral pathogens, their practical applications, and their efficacy against conventional treatments, paving the way for innovative dental therapies. Teucrium polium extracts effectively inhibit oral bacteria causing dental caries, with alcoholic extracts outperforming aqueous extracts. Mouthwashes containing T. polium's effective component prevent tooth decay.

The susceptibility of Teucrium polium essential oil (EO) as an antioxidant for canola oil was studied. Major compounds of the EO were 11-acetoxyeudesman-4-α-ol (26.3%) and α-bisabolol (24.6%). Different concentrations (200, 600 and 1200 ppm) of EO and synthetic antioxidant BHA (200 ppm) were added to canola oil and incubated for 60 days at room temperature. Acid value (AV), peroxide value (PV), carbonyl value (CV), iodine value (IV), total phenolics (TP), total polar compounds (TPC) and oxidative stability index (OSI) of canola oil were determined. Antioxidant capacity of the EO was measured by DPPH and β-carotene–linoleic acid assays. Results exhibited that DPPH and β-carotene–linoleic acid experiment detections on the EO were analogous in high concentrations to those detected on BHA. Moreover, incorporated EO samples had better AV, PV, CV, IV, TP and TPC than control. EO at concentration of 600 ppm indicated higher antioxidant activity in canola oil compared with BHA.