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Recent intensive livestock production has made domestic animals vulnerable to infectious diseases such as foot and mouth disease. Infected animals and nearby animals are culled and then buried or incinerated to prevent the spread of the disease in most countries, including South Korea. The burial of animal carcasses in the soil may produce side effects, such as the production of leachate and odors, and could potentially spread infectious diseases. This study tested YM microorganisms, which mainly contain hyper-thermophilic organisms that can degrade pig carcasses and inactivate pathogenic microorganisms. An experimental burial system installed with an aeration facility was developed, and the decomposition effects of YM microorganisms were verified using pig carcasses. Pig carcasses were almost completely decomposed in 30 days without leachate, and all experimentally inoculated pathogens were completely inactivated by YM microorganisms. The final composites were satisfied with all inspection criteria for using the byproduct as fertilizer after an additional 34 days of composting. In conclusion, the newly developed hyper-thermophilic YM microorganism system may produce biosecure fertilizers from animal carcasses.

The correct management of high-moisture organic waste (HMOW) is crucial to minimize its environmental impact and take advantage of its potential as a valuable resource, thus linking it to the circular economy, sustainable production and recycling. Processes such as anaerobic digestion, composting and, more recently, biodrying have been applied to support the sustainable management of HMOW. However, the latter has not yet been well characterized, so this study focuses on elucidating the behavior of microbial populations and their relationship with physical and chemical conditions during biodrying. In a greenhouse, a semi-static pile with an initial water content of 88%, composed of orange peel waste (80%), sugarcane bagasse (16.5%) and mulch (3.5%) was biodried for 50 days. Biodrying went through three stages: (1) the mesophilic stage, when different microbial populations decompose some organic matter, causing a temperature increase from 25 to 40 °C; (2) the thermophilic stage, in which the highest microbial counts were found, most of which corresponded to the highest temperatures reached and maintained between 40 and 62 °C, and, consequently, to the greatest decrease in water content (from 78 to 41%); and (3) the cooling phase, when the temperature dropped to 23–25 °C. The aeration and mainly the microbial activity were responsible for most of the water evaporation. Microbial activity in biodrying of HMOW ended on day 32, when the humidity was lower than 30% and the water activity (aw) was below 0.8. After that, moisture loss was carried out only by convection and radiation. Obtained biodried organic waste (10% water-content) could be used as an alternative fuel in many industries.

Composting is a promising source of mesophilic and thermophilic microorganisms directly involved in the decay of organic matter. However, there is a paucity of information related to bacterial and fungal diversity in compost and their enzymatic activities during the composting process. In this work, bacterial and fungal diversity during the mesophilic and thermophilic phases of textile waste composting was investigated as a way to explain the physical–chemical results obtained during the composting process. This was accomplished using a next-generation sequencing approach that targets either the 16S rRNA or ITS genomic regions of bacteria and fungi, respectively. It was observed that Proteobacteria, Bacteroidetes, and Actinobacteria were the dominant bacterial phyla present at the mesophilic phase but not at the thermophilic one. Composting textile waste exhibits a sustained thermophilic profile (above 55 °C) that usually precludes fungal activity. Nonetheless, the presence of fungi at the thermophilic phase was observed. Rozellomycota, Basidiomycota, and Ascomycota were the most dominant phyla during both composting phases. Such thermophilic fungi with great ability to decay organic matter could be isolated as pure cultures and used for the bioaugmentation of textile waste composting to achieve an advanced maturity level of textile waste compost.

Microbial communities exhibit intricate interactions underpinned by metabolic dependencies. To elucidate these dependencies, we present a workflow utilizing random matrix theory on metagenome-assembled genomes to construct co-occurrence and metabolic complementarity networks. We apply this approach to a temperature gradient hot spring, unraveling the interplay between thermal stress and metabolic cooperation. Our analysis reveals an increase in the frequency of metabolic interactions with rising temperatures. Amino acids, coenzyme A derivatives, and carbohydrates emerge as key exchange metabolites, forming the foundation for syntrophic dependencies, in which commensalistic interactions take a greater proportion than mutualistic ones. These metabolic exchanges are most prevalent between phylogenetically distant species, especially archaea-bacteria collaborations, as a crucial adaptation to harsh environments. Furthermore, we identify a significant positive correlation between basal metabolite exchange and genome size disparity, potentially signifying a means for streamlined genomes to leverage cooperation with metabolically richer partners. This phenomenon is also confirmed by another composting system which has a similar wide range of temperature fluctuations. Our workflow provides a feasible way to decipher the metabolic complementarity mechanisms underlying microbial interactions, and our findings suggested environmental stress regulates the cooperative strategies of thermophiles, while these dependencies have been potentially hardwired into their genomes during co-evolutions. Microbial communities rely on metabolic interactions to survive in extreme environments. This study shows that rising temperatures increase these interactions, particularly between less related species, highlighting the role of metabolic cooperation in microbial adaptation to thermal stress.

It has been proposed that early bacteria, or even the last universal common ancestor of all cells, were thermophilic. However, research on the origin and evolution of thermophily is hampered by the difficulties associated with the isolation of deep-branching thermophilic microorganisms in pure culture. Here, we isolate a deep-branching thermophilic bacterium from a deep-sea hydrothermal vent, using a two-step cultivation strategy (“Subtraction-Suboptimal”, StS) designed to isolate rare organisms. The bacterium, which we name Zhurongbacter thermophilus 3DAC, is a sulfur-reducing heterotroph that is phylogenetically related to Coprothermobacterota and other thermophilic bacterial groups, forming a clade that seems to represent a major, early-diverging bacterial lineage. The ancestor of this clade might be a thermophilic, strictly anaerobic, motile, hydrogen-dependent, and mixotrophic bacterium. Thus, our study provides insights into the early evolution of thermophilic bacteria. Thermophilic microorganisms can live at high temperatures, but the origin and evolution of this ability are unclear. Here, the authors isolate a thermophilic bacterium from a deep-sea hydrothermal vent, and show it belongs to a major early-diverging lineage whose ancestor was likely also a thermophilic bacterium.

Thermophilic cyanobacteria are prokaryotic photoautotrophic microorganisms capable of growth between 45 and 73 °C. They are typically found in hot springs where they serve as essential primary producers. Several key features make these robust photosynthetic microbes biotechnologically relevant. These are highly stable proteins and their complexes, the ability to actively transport and concentrate inorganic carbon and other nutrients, to serve as gene donors, microbial cell factories, and sources of bioactive metabolites. A thorough investigation of the recent progress in thermophilic cyanobacteria reveals a significant increase in the number of newly isolated and delineated organisms and wide application of thermophilic light-harvesting components in biohybrid devices. Yet despite these achievements, there are still deficiencies at the high-end of the biotechnological learning curve, notably in genetic engineering and gene editing. Thermostable proteins could be more widely employed, and an extensive pool of newly available genetic data could be better utilised. In this manuscript, we attempt to showcase the most important recent advances in thermophilic cyanobacterial biotechnology and provide an overview of the future direction of the field and challenges that need to be overcome before thermophilic cyanobacterial biotechnology can bridge the gap with highly advanced biotechnology of their mesophilic counterparts. Key points • Increased interest in all aspects of thermophilic cyanobacteria in recent years • Light harvesting components remain the most biotechnologically relevant • Lack of reliable molecular biology tools hinders further development of the chassis Graphical Abstract

This study was conducted to evaluate the effect of the sewage sludge treatment method using bio-drying with Ultra-Thermophilic Aerobic Microorganisms (UTAMs). Twelve specific odorous compounds and various sources of bacteria were tested using the sewage sludge treatment method. Sewage sludge was mixed with a seed material and was composted for 47 days. During composting, the temperature was maintained at 80-90oC. The concentrations of the 12 specific odorous compounds after composting did not exceed the allowable exhaust standard for odor. In terms of the bacterial community number after composting, the thermophile bacterial number was 60% of the total bacterial number. The thermophile bacterial ratio after composting increased by 23% compared to the initial composting. The 16S rRNA gene demonstrated that the change in the bacterial community structure was coupled with shifts in the bio-drying process. Therefore, both stable composting operation and economic benefit can be expected when an ultra-thermophilic composting process is applied to sewage sludge.

Thermal stability is one of the most desirable characteristics in the search for novel lipases. The search for thermophilic microorganisms for synthesising functional enzyme biocatalysts with the ability to withstand high temperature, and capacity to maintain their native state in extreme conditions opens up new opportunities for their biotechnological applications. Thermophilic organisms are one of the most favoured organisms, whose distinctive characteristics are extremely related to their cellular constituent particularly biologically active proteins. Modifications on the enzyme structure are critical in optimizing the stability of enzyme to thermophilic conditions. Thermostable lipases are one of the most favourable enzymes used in food industries, pharmaceutical field, and actively been studied as potential biocatalyst in biodiesel production and other biotechnology application. Particularly, there is a trade-off between the use of enzymes in high concentration of organic solvents and product generation. Enhancement of the enzyme stability needs to be achieved for them to maintain their enzymatic activity regardless the environment. Various approaches on protein modification applied since decades ago conveyed a better understanding on how to improve the enzymatic properties in thermophilic bacteria. In fact, preliminary approach using advanced computational analysis is practically conducted before any modification is being performed experimentally. Apart from that, isolation of novel extremozymes from various microorganisms are offering great frontier in explaining the crucial native interaction within the molecules which could help in protein engineering. In this review, the thermostability prospect of lipases and the utility of protein engineering insights into achieving functional industrial usefulness at their high temperature habitat are highlighted. Similarly, the underlying thermodynamic and structural basis that defines the forces that stabilize these thermostable lipase is discussed.Key points• The dynamics of lipases contributes to their non-covalent interactions and structural stability.• Thermostability can be enhanced by well-established genetic tools for improved kinetic efficiency.• Molecular dynamics greatly provides structure-function insights on thermodynamics of lipase.

The geosphere and the microbial biosphere have co-evolved for ~3.8 Ga, with many lines of evidence suggesting a hydrothermal habitat for life’s origin. However, the extent that contemporary thermophiles and their hydrothermal habitats reflect those that likely existed on early Earth remains unknown. To address this knowledge gap, 64 geochemical analytes were measured and 1022 metagenome-assembled-genomes (MAGs) were generated from 34 chemosynthetic high-temperature springs in Yellowstone National Park and analysed alongside 444 MAGs from 35 published metagenomes. We used these data to evaluate co-variation in MAG taxonomy, metabolism, and phylogeny as a function of hot spring geochemistry. We found that cohorts of MAGs and their functions are discretely distributed across pH gradients that reflect different geochemical provinces. Acidic or circumneutral/alkaline springs harbor MAGs that branched later and are enriched in sulfur- and arsenic-based O 2 -dependent metabolic pathways that are inconsistent with early Earth conditions. In contrast, moderately acidic springs sourced by volcanic gas harbor earlier-branching MAGs that are enriched in anaerobic, gas-dependent metabolisms (e.g. H 2 , CO 2 , CH 4 metabolism) that have been hypothesized to support early microbial life. Our results provide insight into the influence of redox state in the eco-evolutionary feedbacks between thermophiles and their habitats and suggest moderately acidic springs as early Earth analogs. Life may have originated in a hydrothermal habitat, but the extent that contemporary thermophilic microbes and their environments reflect those on early Earth is unclear. Here, Colman et al. evaluate covariation in microbial taxonomy, metabolism and phylogeny as a function of hot spring geochemistry, suggesting moderately acidic springs as early Earth analogs.