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The aim of the research is to investigate the impact of laser operation parameters on the LIG (laser-induced graphene) process. It focuses on evaluating the feasibility of using the induced conductive layers to create antenna circuits that are dedicated to radio-frequency identification (RFID) technology. Given the specific design of textile RFIDtex transponders, applying the LIG technique to fabricate antenna modules on a flexible substrate (e.g., Kapton) opens new possibilities for integrating RFID labels with modern materials and products. The paper analyses the efficiency of energy and data transmission in the proposed innovative UHF RFIDLIG tags. The signal strength, read range, and effectiveness are estimated in the experimental setup, providing key insights into the performance of the devices. Based on the obtained results, it can be concluded that changes in laser cutting parameters, the size of the induced graphene layer, and the method of fixing the Kapton substrate significantly affect the quality of the cutting/engraving components and the conductivity of burned paths. However, these changes do not directly affect the correct operation of the RFIDLIG transponders, owing to the fact that these structures are resistant to external impacts. Nevertheless, an increased range of data readout from the RFIDLIG tags can be achieved by using graphene paths with higher conductivity. The obtained results confirm the validity of the proposed concept and provide a foundation for further research on adapting the LIG method to automated logistics, ultimately leading to the development of more versatile and innovative solutions for identification processes.

The results of the study of strip parametric passive radio transponders are presented. Stripline parametric passive radio transponders are made in the form of a system of three parametric generators connected to the pump signal strip antenna and the response signal strip antenna. The results of numerical and field experiments are presented for a pump signal frequency of 800 MHz and a response signal of 400 MHz, which are in good agreement.

A systematic review and meta-analysis was conducted to evaluate the appropriate tag:fish size ratio when tagging juvenile salmonids (genera Oncorhynchus, Salmo, and Salvelinus). The review yielded 18 publications with 211 control and treatment groups reporting results from laboratory studies on the effects of passive integrated transponder (PIT) tags, plus a small number of additional studies on acoustic transmitters. A meta-analysis of the PIT tagging studies showed significant heterogeneity among studies with respect to mortality. Meta-regression revealed that juvenile salmonid mortality increased curvilinearly with the tag:fish length ratio, indicating that mortality risk is rapidly enhanced as smaller fish or larger tag sizes are used. The tag:fish length ratio effect on daily length or mass gain increased linearly. The results provide an estimate of the effects of the tag:fish length ratio on mortality and growth parameters in juvenile salmonids. Based on this, we suggest that researchers can follow best practices for tagging juvenile salmonids with tags that are not greater than 17.5% of fish total length (TL). This equates a minimum size threshold of 131 mm TL for tagging salmonids with 23-mm PIT tags, and 69 mm TL with 12-mm PIT tags. This information can assist researchers, managers, and welfare agencies striving to use the best possible evidence to make informed decisions regarding fish tagging.

Body temperature is a valuable parameter in determining the wellbeing of laboratory animals. However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias. Here we compared two methods to assess body temperature in mice, namely implanted radio frequency identification (RFID) temperature transponders (method 1) to non-contact infrared thermometry (method 2) in 435 mice for up to 7 days during normothermia and lipopolysaccharide (LPS) endotoxin-induced hypothermia. There was excellent agreement between core and surface temperature as determined by method 1 and 2, respectively, whereas the intra- and inter-subject variation was higher for method 2. Nevertheless, using machine learning algorithms to determine temperature-based endpoints both methods had excellent accuracy in predicting death as an outcome event. Therefore, less expensive and cumbersome non-contact infrared thermometry can serve as a reliable alternative for implantable transponder-based systems for hypothermic responses, although requiring standardization between experimenters.

This paper details the design and implementation of a harmonic frequency-modulated continuous-wave (FMCW) radar system, specialized in detecting harmonic tags and achieving precise range estimation. Operating within the 2.4–2.5 GHz frequency range for the forward channel and 4.8–5.0 GHz for the backward channel, this study delves into the various challenges faced during the system’s realization. These challenges include selecting appropriate components, calibrating the system, processing signals, and integrating the system components. In addition, we introduce a single-layer passive harmonic tag, developed specifically for assessing the system, and provide an in-depth theoretical analysis and simulation results. Notably, the system is characterized by its low power consumption, making it particularly suitable for short-range applications. The system’s efficacy is further validated through experimental evaluations in a real-world indoor environment across multiple tag positions. Our measurements underscore the system’s robust ranging accuracy and its ability to mitigate self-interference, showcasing its significant potential for applications in harmonic tag detection and ranging.

This paper presents a novel passive Schottky-diode frequency doubler equipped with an on-off keying (OOK) modulation port to be used in harmonic transponders for both identification and sensing applications. The amplitude modulation of the second-harmonic output signal is achieved by driving a low-frequency MOSFET, which modifies the dc impedance termination of the doubler. Since the modulation signal is applied to the gate port of the transistor, no static current is drained. A proof-of-concept prototype was manufactured and tested, operating at 1.04 GHz. An on/off ratio of 23 dB was observed in the conversion loss of the doubler for an available input power of −10 dBm. The modulation port of the circuit was excited with a square wave (fm up to 15 MHz), and the measured sidebands in the spectrum featured a good agreement with the theory. Then, the doubler was connected to a harmonic antenna system and tested in a wireless experiment for fm up to 1 MHz, showing an excellent performance. Finally, an experiment was conducted where the output signal of the doubler was modulated by a reed switch used to measure the rotational speed of an electrical motor. This work opens the door to a new class of frequency doublers, suitable for ultra low-power harmonic transponders for identification and sensing applications.

Modern infrastructure, such as dense urban areas and underground tunnels, can effectively block all GPS signals, which implies that effective position triangulation will not be achieved. The main problem that is addressed in this project is the design and implementation of an accurate vehicle location system using radio-frequency identification (RFID) technology in combination with GPS and the Global system for Mobile communication (GSM) technology, in order to provide a solution to the limitation discussed above. In essence, autonomous vehicle tracking will be facilitated with the use of RFID technology where GPS signals are non-existent. The design of the system and the results are reflected in this paper. An extensive literature study was done on the field known as the Internet of Things, as well as various topics that covered the integration of independent technology in order to address a specific challenge. The proposed system is then designed and implemented. An RFID transponder was successfully designed and a read range of approximately 31 cm was obtained in the low frequency communication range (125 kHz to 134 kHz). The proposed system was designed, implemented, and field tested and it was found that a vehicle could be accurately located and tracked. It is also found that the antenna size of both the RFID reader unit and RFID transponder plays a critical role in the maximum communication range that can be achieved.

In laboratory fish research, the zebrafish Danio rerio (Cyprinidae) represents the equivalent of the mouse in mammalian research. This species has become a major model for studies in developmental and behavioural genetics, neurophysiology, biomedicine, ecotoxicology, and behavioural and evolutionary ecology. To meet the need for accurate and reproducible data in both fundamental and applied sciences, it is of primary importance to be able to tag and/or recognize individual zebrafish. However, classic methods used in fish ecology and aquaculture are generally difficult to apply to such small fish. Recently, various new tagging methods have been developed. This paper presents a first review of current identification and marking methods applied to zebrafish, from external observation methods (such as skin pattern recognition, fin clipping, scale regeneration, colour and transgenic methods) to the most advanced technological developments in electronic (low- and high- radio-frequencies PIT tags, microchip) and image analysis methods (video tracking). This review aims to help researchers and zebrafish facility managers select the identification method (ID) best adapted to their needs. The main characteristics of each ID method are examined (including detection range, durability, speed and repetitiveness, ID code combination, size dependence and ethical considerations), and their pros and cons are summarized in a decision table to help select the most appropriate option for a research or management program. Finally, contextual applications of these ID methods and future developments are discussed.