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Background The extremely hazardous metal cadmium (Cd) restricts plant growth and interferes with many morphological, biochemical and physiological functions. One biotechnology strategy is the application of fungi to eliminate harmful pollutants from the environment. Methodology Trichoderma viride strain RA1 is employed to investigate its prospective for Cd scavenging and promoting wheat ( Triticum aestivum L.) growth. The colony diameter and mycelial dry weights (dwt) of T. viride were examined. Also, a randomized pot experiment was conducted to mitigate the Cd harmful effects in wheat plants using T. viride RA1grown under Cd-stressed (200 mg/L) conditions. Results The results revealed that with increasing Cd conc., colony diameter and mycelial dwt of T. viride decreased. Also, various phenotypic, physiological and biochemical characteristics were evaluated. Cd content in T. aestivum shoots and roots were appraised. Regarding osmoprotectants, the highest increase in proline content (16.14%), glycine betaine (24.95%) and protein (28.07%) was detected in T. aestivum plants with T. viride RA1 beneath Cd imposition. The MP-AES results indicated a greater buildup of Cd in the roots of plants inoculated with T. viride RA1 than non-inoculated ones. Our data suggest that T. viride can be utilized to increase total antioxidant capacity and osmoprotectant levels while decreasing malondialdehyde, electrolyte leakage, in addition to H 2 O 2 levels in order to lessen the detrimental effects of Cd on T. aestivum plants. Conclusion According to our findings, T. viride RA1 may function as a bio inoculant, encouraging T. aestivum development under Cd stress, thereby assisting sustainable farming methods. Key points Increasing Cd concentration led to a decrement in colony diameter and mycelial dry weight of T. viride . Cd adversely affects T. aestivum growth and causes comparable changes in its physio-biochemical responses. T. viride RA1 function as a bio inoculant, encouraging T. aestivum development in the presence of Cd stress. The growth traits and chlorophyll of T. aestivum treated with Cd were significantly increased by T. viride RA1 inoculation. T. viride RA1 contributed to the increase of antioxidant capacity and alleviated the stress markers in T. aestivum.

Fungal pathogens cause over a billion human infections annually, leading to more than 1.6 million deaths each year. The scarcity of available antifungal drugs intensifies the public health threat posed by human pathogenic fungal infections. Therefore there is a critical demand for novel, safe, and effective antifungal agents. Although chitinases are established as effective antifungal agents against phytopathogenic fungi, research on their activity against human pathogenic fungi is limited. The present study seeks to investigate the anti-inflammatory and antifungal activity of bacterial and fungal chitinase against human pathogenic fungi. The antifungal efficacy of bacterial chitinase from Streptomyces griseus , fungal chitinase from Trichoderma viride , and a combination of both was determined by calculating the inhibition percentage in fungal growth, indicated by the reduction in the dry mass of the fungi. Additionally, the anti-inflammatory activity of these chitinases was assessed by measuring the inhibition of albumin denaturation. Results revealed that chitinases exhibited greater antifungal activity compared to the standard. Notably, bacterial chitinase demonstrated higher effectiveness than fungal chitinase against Aspergillus fumigatus , while the bacterial and fungal chitinase had similar effects against different Cryptococcus neoformans and Candida species . The combination of bacterial and fungal chitinase demonstrated the highest antifungal activity against all tested fungi. Furthermore, the anti-inflammatory activity indicated that chitinases prevented 98% of albumin denaturation, marking the first study reporting the anti-inflammatory role of chitinases in preventing albumin denaturation. Additional in-vivo studies are necessary to explore the antifungal activity of chitinases against human pathogenic fungi and investigate the anti-inflammatory mechanisms of chitinase.

Sesquiterpene synthases in Trichoderma viride have been seldom studied, despite the efficiency of filamentous fungi for terpenoid production. Using the farnesyl diphosphate-overexpressing Saccharomyces cerevisiae platform to produce diverse terpenoids, we herein identified an unknown sesquiterpene synthase from T. viride by genome mining and determined the structure of its corresponding products. One new 5/6 bicyclic sesquiterpene and its esterified derivative were characterised by GC–MS and 1D and 2D NMR spectroscopy. To the best of our knowledge, this is the first well-identified sesquiterpene synthase from T. viride to date.

The obstacle associated with continuous cropping is an important problem in the production of muskmelon (Cucumis melo L.). The allelochemicals from root exudates play an active role in root–microbe communication. The primary objective of this study was to delve into the impact of root exudates and the continuous cultivation of muskmelon on the growth and colonization patterns of Trichoderma viride T23. It was observed that the root exudates of muskmelon significantly promoted mycelial growth and the sporulation of Trichoderma viride T23 at concentrations of 0.05, 0.1 g·mL−1, while at a concentration of 0.05 g·mL−1, the enzyme activities of β-glucosidase, chitinase and cellulase were 12.34, 13.23, and 17.85 U·mL−1, respectively, which were higher than those of the control. With increasing concentrations of root exudates, the hyphal growth, spore germination, and the three enzyme activities of Trichoderma viride T23 were decreased. The findings from the pot experiments revealed that the total phenolic acid content in the soil of replanted muskmelon demonstrated a trend of escalating over the course of the first growth cycle of continuous cropping to the fourth growth cycle of continuous cropping. The population density in the rhizosphere soil of Trichoderma viride T23 in the first growth cycle and the second growth cycle of continuous cropping shows a significant difference compared with other treatments, which led to statistically significant increments of stem diameter, leaf area, fresh weight, dry weight and SPAD index. It is necessary to increase the dose of the beneficial microorganism or degrade the phenolics in the rhizosphere soil to promote effectiveness while increasing the growth cycles of continuous cropping.

With the increasing use of chemical fertilizers, negative environmental impacts have greatly increased as a result from agricultural fields. The fungus Trichoderma viride used as a biofertilizer can efficiently reduce nitrous oxide (N 2 O) emissions from subtropical tea fields in southern China. In this paper, it was further found that T. viride biofertilizer could alleviate nitrogen (N) leaching in tea fields. Gross N leaching was 1.51 kg ha −1  year −1 with no external fertilizer input, but when 225 kg N ha −1  year −1 was applied, it increased to 12.38 kg ha −1  year −1 using T. viride biofertilizer but 53.46 kg ha −1  year −1 using urea. Stepwise linear regression analysis identified the factors responsible for N leaching to be soil nitrate concentration and soil interflow, simulated here using the water balance simulation model (WaSiM-ETH). Finally, mass-scale production of T. viride biofertilizer from waste reutilization using sweet potato starch wastewater and rice straw was found to be cost-effective and feasible. These procedures could be considered a best management practice to reduce N leaching from tea fields in subtropical areas of central China and to reduce pollution from other agricultural waste products.

Environmental pollution and the exploitation of fossil-based products are topical issues that should be a matter of concern to the global population. The production of bio-based substances from waste biomass is a way to reduce the consumption of fossil fuels and limit environmental pollution. Enzymatic catalysed saccharification of cellulose is an important step for the bio-conversion of biomass such as waste paper into glucose that could be utilized as a feedstock for the production of value added bioproducts and this process can also be considered as an alternative route of waste management. During this study, fresh cellulase enzyme from Trichoderma viride was incubated separately with seven different waste paper materials during twelve successive incubation periods of 2 h each. The amount of sugar released from each paper material during each incubation period was determined. The highest sugar concentration released from each paper materials was produced during the first incubation period except the filter paper for which the highest amount of sugar was produced during the 9th period of incubation. During these optimum sugar producing incubation periods the highest total sugar concentration was released from brown envelope paper (3.3 mg.mL-1 followed by foolscap paper (3.0 mg.mL-1) and office paper (2.8 mg.mL-1) while the lowest amount of sugar was released from Pick ’n Pay paper (0.6 mg.mL-1). The relative saccharification percentage was also calculated which showed that filter paper produced the highest amounts of sugar followed by newspaper, and foolscap paper with advertising paper from a retailer. Pick ’n Pay offered the highest resistance towards cellulase catalysed bio-conversion into sugar.

Nitroaromatic and nitroamine compounds such as 2,4,6-trinitrotoluene (TNT) are teratogenic, cytotoxic, and may cause cellular mutations in humans, animals, plants, and microorganisms. Microbial-based bioremediation technologies have been shown to offer several advantages against the cellular toxicity of nitro-organic compounds. Thus, the current study was designed to evaluate the ability of Trichoderma viride to degrade nitrogenous explosives, such as TNT, by microbiological assay and Gas chromatography–mass spectrometry (GC–MS) analysis. In this study, T. viride fungus was shown to have the ability to decompose, and TNT explosives were used at doses of 50 and 100 ppm on the respective growth media as a nitrogenous source needed for normal growth. The GC/MS analysis confirmed the biodegradable efficiency of TNT, whereas the initial retention peak of the TNT compounds disappeared, and another two peaks appeared at the retention times of 9.31 and 13.14 min. Mass spectrum analysis identified 5-(hydroxymethyl)-2-furancarboxaldehyde with the molecular formula C6H6O3 and a molecular weight of 126 g·mol−1 as the major compound, and 4-propyl benzaldehyde with a formula of C10H12O and a molecular weight of 148 g mol−1 as the minor compound, both resulting from the biodegradation of TNT by T. viride. In conclusion, T. viride could be used in microbial-based bioremediation technologies as a biological agent to eradicate the toxicity of the TNT explosive. In addition, future molecular-based studies should be conducted to clearly identify the enzymes and the corresponding genes that give T. viride the ability to degrade and remediate TNT explosives. This could help in the eradication of soils contaminated with explosives or other toxic biohazards.

Trichoderma reesei is the main industrial source of cellulases and hemicellulases used to depolymerize biomass to simple sugars that are converted to chemical intermediates and biofuels, such as ethanol. We assembled 89 scaffolds (sets of ordered and oriented contigs) to generate 34 Mbp of nearly contiguous T. reesei genome sequence comprising 9,129 predicted gene models. Unexpectedly, considering the industrial utility and effectiveness of the carbohydrate-active enzymes of T. reesei , its genome encodes fewer cellulases and hemicellulases than any other sequenced fungus able to hydrolyze plant cell wall polysaccharides. Many T. reesei genes encoding carbohydrate-active enzymes are distributed nonrandomly in clusters that lie between regions of synteny with other Sordariomycetes. Numerous genes encoding biosynthetic pathways for secondary metabolites may promote survival of T. reesei in its competitive soil habitat, but genome analysis provided little mechanistic insight into its extraordinary capacity for protein secretion. Our analysis, coupled with the genome sequence data, provides a roadmap for constructing enhanced T. reesei strains for industrial applications such as biofuel production.

Encapsulated bioactive agents applied to the Lactuca sativa L. present an innovative approach to stimulate the production of plant secondary metabolites increasing its nutritive value. Calcium and copper ions were encapsulated in biopolymeric microparticles (microspheres and microcapsules) either as single agents or in combination with biocontrol agents, Trichoderma viride spores, a fungal plant growth mediator. Both, calcium and copper ions are directly involved in the synthesis of plant secondary metabolites and alongside, Trichoderma viride can provide indirect stimulation and higher uptake of nutrients. All treatments with microparticles had a positive effect on the enhancement of plant secondary metabolites content in Lactuca sativa L. The highest increase of chlorophylls, antioxidant activity and phenolic was obtained by calcium-based microparticles in both, conventionally and hydroponically grown lettuces. Non-encapsulated fungus Trichoderma viride enhanced the synthesis of plant secondary metabolites only in hydroponics cultivation signifying the importance of its encapsulation. Encapsulation proved to be simple, sustainable and environmentally favorable for the production of lettuce with increased nutritional quality, which is lettuce fortified with important bioactive compounds.

Straw is one of the most abundant stock of renewable biomass from crop production. However, its utilization efficiency is still very low. Although co-cultivation of fungi increases the degrading rate, the co-cultivation condition needs to be optimized. To optimize the co-culture condition of Phanerochaete chrysosporium and Trichoderma viride degrading rice straw, we first tested the antagonistic characteristic between the fungi. The results showed that the best co-culture pattern was to first inoculate P. chrysosporium and culture for 4 days, then inoculate T. viride , and co-culture the two fungi for 4 days. The optimum fermentation condition was 14% (w/v) of inoculum concentration, the equivalent inoculation of the fungi, culture temperature at 30 °C, and 1:1.4 for solid-liquid ratio. Under the optimum condition, the degradation ratios of lignin and cellulose were 26.38% and 33.29%, respectively; the soluble carbon content in the culture product was 23.07% (w/v). The results would provide important reference information for the efficient utilization of rice straw to produce more accessible energy resources, such as ethanol and glucose.