Explore the vast range of titles available.

Background Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field, has critical limitations for detecting and quantifying structural variants such as large deletions (greater than approximately 100 base pairs), inversions, and translocations. Results To overcome these limitations, we have developed a Uni-Directional Targeted Sequencing methodology, UDiTaS, that is quantitative, removes biases associated with variable-length PCR amplification, and can measure structural changes in addition to small insertion and deletion events (indels), all in a single reaction. We have applied UDiTaS to a variety of samples, including those treated with a clinically relevant pair of S. aureus Cas9 single guide RNAs (sgRNAs) targeting CEP290 , and a pair of S. pyogenes Cas9 sgRNAs at T-cell relevant loci. In both cases, we have simultaneously measured small and large edits, including inversions and translocations, exemplifying UDiTaS as a valuable tool for the analysis of genome editing outcomes. Conclusions UDiTaS is a robust and streamlined sequencing method useful for measuring small indels as well as structural rearrangements, like translocations, in a single reaction. UDiTaS is especially useful for pre-clinical and clinical application of gene editing to measure on- and off-target editing, large and small.

My home in Mumbai. And when I'm in my hometown, Dehradun, it's a place called 'Machan' which is very peaceful and quiet.

The story revolves around three best friends, Sid ([Arshad Warsi]), John ([Aashish Choudhary]) and Amit (Yash Tonk). Their life is full of fun, frolic and adventure, but it takes a twist when John meets Sheetal ([Udita Goswami]), who wishes to hook him for all his wealth. Sid and Amit understand her intentions and decide to bring John back. From there begins the roller coaster of rib-tickling comedy.

The musical about starcrossed lovers is a slapstick comedy loaded with (mostly) mindless gags. Those who are looking for something intelligent and a story that makes sense should avoid this film, which stars Tusshar Kapoor and a host of familiar supporting actors. This is a movie that will appeal more to those who enjoy the fast-food films like Housefull, No Entry and Yamla Pagla Deewana. The funny moments and nice musical numbers balance out a silly story and over-acting. Watch this, but with your brain switched off.