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To better understand the relationship between the environmental variables and microbial communities of activated sludge, we took winter samples from different biological treatment units (anaerobic, oxic, etc) from the WWTP’s of a number of Chinese cities. Differences in influent organic components and activated sludge microbial communities were identified by gas chromatography-mass spectrometry and high-throughput sequencing technology, respectively. Liquid nitrogen grinding pretreatment of samples was found to aid in the obtaining of a more bio-diversified sample. Influent type and dissolved oxygen concentration influenced the activated sludge microbial community structure. Nitrospira , Caldilineaceae and Anaerolineaceae were highly related to domestic wastewater treatment systems, whereas Thauera was the most abundant putative refractory aromatic hydrocarbon decomposer found in industrial wastewater treatment systems. Within the influent composition, we speculate that Thauera, Macellibacteroides and Desulfomicrobium are the key functional genera of the anaerobic environment of the textile dyeing industry wastewater treatment systems, whilst Thauera and Thiobacillus are key functional microbes in fine chemical wastewater treatment systems.

Organic dyes are one of the most commonly discharged pollutants in wastewaters; however, many conventional treatment methods cannot treat them effectively. Over the past few decades, we have witnessed rapid development of nanotechnologies, which offered new opportunities for developing innovative methods to treat dye-contaminated wastewater with low price and high efficiency. The large surface area, modified surface properties, unique electron conduction properties, etc. offer nanomaterials with excellent performances in dye-contaminated wastewater treatment. For examples, the agar-modified monometallic/bimetallic nanoparticles have the maximum methylene blue adsorption capacity of 875.0 mg/g, which are several times higher than conventional adsorbents. Among various nanomaterials, the carbonaceous nanomaterials, nano-sized TiO 2 , and graphitic carbon nitride (g-C 3 N 4 ) are considered as the most promising nanomaterials for removing dyes from water phase. However, some challenges, such as high cost and poor separation performance, still limit their engineering application. This article reviewed the recent advances in the nanomaterials used for dye removal via adsorption, photocatalytic degradation, and biological treatment. The modification methods for improving the effectiveness of nanomaterials are highlighted. Finally, the current knowledge gaps of developing nanomaterials on the environmental application were discussed, and the possible further research direction is proposed.

To understand the seasonal variation of the activated sludge (AS) bacterial community and identify core microbes in different wastewater processing systems, seasonal AS samples were taken from every biological treatment unit within 4 full-scale wastewater treatment plants. These plants adopted A2/O, A/O and oxidation ditch processes and were active in the treatment of different types and sources of wastewater, some domestic and others industrial. The bacterial community composition was analyzed using high-throughput sequencing technology. The correlations among microbial community structure, dominant microbes and process performance were investigated. Seasonal variation had a stronger impact on the AS bacterial community than any variation within different wastewater treatment system. Facing seasonal variation, the bacterial community within the oxidation ditch process remained more stable those in either the A2/O or A/O processes. The core genera in domestic wastewater treatment systems were Nitrospira , Caldilineaceae , Pseudomonas and Lactococcus . The core genera in the textile dyeing and fine chemical industrial wastewater treatment systems were Nitrospira , Thauera and Thiobacillus .

With the unique advantages of cost-effectiveness and low energy consumption, constructed wetlands (CWs) are commonly used for treatment of secondary municipal wastewaters. Over the last decades, CWs have gained increased popularity for treating agricultural runoff and agro-industrial wastewater. This review highlights the practice, application, and research on wetland technology, placing them in the overall context of the need for reliable and sustainable solutions to managing agricultural runoff and agro-industrial wastewater. A critical assessment of the performance and effectiveness of wetland systems for removing various contaminants of importance to agriculture is presented. The design parameters and operational conditions affecting the efficiency of contaminant removal in CWs receiving agricultural runoff and agro-industrial wastewater are also discussed. The role of proper pretreatment, artificial aeration, effluent recirculation, in-series design, and microbial dynamics on the enhancement of treatment is provided. Challenges and perspectives for future research on agricultural treatment wetlands are also addressed.

The performance of catalytic ability of MFe 2 O 4 /MoS 2 in the ozonation process was investigated in this work. The synthesized MnFe 2 O 4 /MoS 2 was optimize prepared and then characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photo-electron spectroscopy, and magnetic saturation strength. The results showed that when C phenol  = 200 mg/L, initial pH = 9.0, Q  = 0.10 L/min, and C MnFe2O4/MoS2  = 0.10 g/L, MnFe 2 O 4 /MoS 2 addition improved the degradation efficiency of phenol by 20.0%. The effects of pH, catalyst dosage, and inorganic ions on the phenol removal by the MnFe 2 O 4 /MoS 2 catalytic ozonation were investigated. Five cycle experiments proved that MnFe 2 O 4 /MoS 2 had good recyclability and stability. MnFe 2 O 4 /MoS 2 also showed good catalytic performance in the treatment of coal chemical wastewater pesticide wastewater. The MnFe 2 O 4 doped with MoS 2 could provide abundant surface active sites for ozone and promote the stable cycle of Mn 2+ /Mn 3+ and Fe 2+ /Fe 3+ , thus generating large amounts of •OH and improving the degradation of phenol by ozonation. The MnFe 2 O 4 /MoS 2 /ozonation treatment system provides a technical reference and theoretical basis for industrial wastewater treatment.

Industrial waste as novel conductive mediator was applied for wastewater treatment as a novel strategy for both waste recycling and sustainable development of wastewater treatment. In this study, nanoscale zero valent iron-loaded fly ash-based activated carbon (nZVI@FABAC) was prepared and applied to enhancing activated sludge (AS) process for coal chemical wastewater (CCW) treatment. The results demonstrated that the removal efficiencies of COD and total phenols (TPh) in nZVI@FABAC/AS process reached about 83.96 and 85.17%, which increased 52.51 and 31.52% compared with the single AS process, respectively. And the acute toxic unit value of CCW was reduced by 88.24% after nZVI@FABAC/AS process treatment. The various functional bacteria including phenol-degrading bacteria ( Comamonas and Acinetobacter ), electroactive bacteria ( Geobacter ), and iron reduction bacteria ( Geothrix ) were enriched in the nZVI@FABAC/AS process, which provided various electron transfer pathways to improve the degradation of toxic organics in CCW. Accordingly, nZVI@FABAC/AS process provided a promising and sustainable way for industrial wastewater treatment.

The milk processing industry is one of the world's staple industries, thus the treatment possibilities of dairy effluents have been attracting more and more attention. The purpose of the paper is to review contemporary research on dairy wastewater. The origin, categories, as well as liquid by-products and general indicators of real dairy wastewater are described. Different procedures applied for dairy wastewater management are summarised. Attention is focused on in-factory treatment technologies with the emphasis on biological processes. Aerobic and anaerobic methods with both their advantages and disadvantages are discussed in detail. Consecutive anaerobic and aerobic systems are analysed, too. Finally, future research niches are identified.

The phytotoxicity effect of olive mill wastewater (OMWW) treated in a combined system regrouping pretreatment by filtration on olive stones and coagulation-flocculation, and anaerobic digestion (AD) on seed germination of maize and tomato was evaluated through germination tests in petri dishes and growth tests in pots. Three samples, referenced as AD-40, AD-60, and AD-80, were collected from the anaerobic reactor operating with an influent at 40, 60, and 80% OMWW/water (% v/v). Concentrations between 25 and 100% were used for maize and between 5 and 25% were used for tomato using raw and pretreated samples, while anaerobic samples were used without dilution. For maize, 100% and 75% OMWW were very phytotoxic and completely prohibited seed germination, while phytotoxicity was decreased following dilution at 25% and 50% OMWW. Maize germinability was found highly enhanced when watered with anaerobic samples. For tomato, high dilution was required to reduce the phytotoxicity of raw and pretreated OMWW and a high relative germination percentage was registered at 5, 10, and 15% OMWW, while for samples anaerobically treated, a high phytotoxicity is still observed. Growth tests, showed more favorable results for maize watered with raw and pretreated samples at 25% OMWW and with biological samples. For tomato and with the exception of 25% OMWW and AD-80, seeds respond positively to all samples. It was concluded that if the OMWW will be used for irrigating maize, it could be directly used after anaerobic digestion, while for tomato further dilution is required. The phenolic profile analysis of the tested samples coupled with the results of the germination tests showed that the OMWW phytotoxicity appears to be determined by not only the monomeric phenols but also by other toxic components unaffected by the applied treatments.

Surgical cotton production has drastically been increased in the past few years due to excessive use by medical health professionals especially in countries like India, which is among the top three exporters of cotton worldwide. The effluent generated from surgical cotton industries differ from textile effluents by the conspicuous absence of dyeing chemicals. This wastewater has a high concentration of suspended particles, COD, dissolved ions, organic carbon, and alkaline pH. Several studies have been published on the treatment of textile effluents and the degradation of dyeing chemicals, while the treatment studies on surgical cotton wastewater have been rarely reported in spite of their potential to cause pollution in receiving land/water bodies. Activated sludge microbes have been extensively studied and well documented in the treatment of several industrial effluent but does not match to the production of valuable biomass from algae. The global energy demand has prompted the scientific community to investigate and explore the possibility of using algae for energy production with simultaneous wastewater treatment. To the best of the authors’ knowledge, no research articles have been published which compare the effectiveness of activated sludge microorganisms, microalgae, and macroalgae in removing contaminants from real wastewater. To date, there is a knowledge gap in understanding and selecting the right choice of biological system for effective and economical effluent treatment. In an attempt to minimize this gap, carbon removal by microalgae, macroalgae, and activated sludge microbes were investigated on real effluent from surgical cotton industries. It was observed that the strain of Chlorella vulgaris could dissipate 83% of COD from real wastewater, while consortia of macroalgae (consisting predominantly of Ulvaceae and Chaetomorpha ) and activated sludge microbes could remove 81% and 69% of the carbon, respectively. The microalgal growth (in terms of wet weight) increased from 0.15 to 0.3 g, whereas the macroalgal wet weight increased from 1.5 to 3 g in over 7 days of batch experiments conducted in triplicates. This indicated the superlative performance of microalgae over activated sludge microbes in carbon dissipation.

This study aims to investigate the feasibility of treating olive mill waste water (OMWW) by activated sludge pilot (AS) after its high dilution (1%) by urban waste water (UWW) and to study the effect of polyphenol compounds on the biomass during the treatment. Specific oxygen uptake rate (SOUR), mixed liquor volatile suspended solids (MLVSS), chemical oxygen demand (COD) and total polyphenols, were followed up over 100 days. In spite of the polyphenols' high concentration (up to 128 mg·L−1), successful biomass growth of 7.12 g MLVSS.L −1 and activity were achieved. Most of the bacteria (Pseudomonas sp., Klebsiella oxytoca, Citrobacter fereundii, Escherichia coli and Staphylococcus sp.) and fungi (Trichoderma sp., Rhizopus sp., Aspergillus niger, Penicillium sp., Fusarium sp., Alternaria) identified in the aerobic basin during the stabilization stage were known to be resistant to OMWW and showed effective adaptation of the biomass to polyphenols in high concentration. COD and polyphenols were highly eliminated (90%, 92% respectively). The sludge volume index in the pilot settling tank was almost constant at around 120 mL.g −1. This suggests the possibility of managing OMWW by simple injection at a given percentage in already functioning conventional AS treating UWW.