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Using low-quality maize, resulting from insect pests and fungal attack, for formulating feed reduces chicken performance. This study evaluated the effectiveness of hermetic storage bags to keep insect pest and mycotoxin levels in check in yellow maize. The study was conducted in storehouses at three poultry farms in Dormaa Ahenkro, Bono Region, Ghana. The experiment was set up in a Randomized Complete Block Design with ZeroFly® Hermetic (ZFH), Purdue Improved Crop Storage (PICS), and Polypropylene (PP) bags as treatments. In each treatment, twelve 50 kg samples of untreated maize were each put in 100 kg capacity bags. Two bags in each treatment were destructively sampled monthly for 6 months. The number of insects was significantly higher in the PP bag (161.00 ± 4.25), compared to the PICS and ZFH bags: 7.00 ± 0.29 and 4.50 ± 0.76, respectively. The PICS and ZFH bags had less insect damage and lower weight loss than the PP bags. Aflatoxin and fumonisin levels were below the recommended safe thresholds of 15 ppb and 4 ppm, respectively, in all the bags. With the exception of ash, proximate analyses were higher for all variables in the PICS and ZFH bags. The study showed that PICS and ZFH bags conserved maize quality better than the PP bag.

This study combined moist airtight storage of moist grain with pig feed fermentation. Starter cultures with the potential to facilitate both technologies were added to airtight stored moist crimped cereal grain, and the impact on storage microflora and the quality of feed fermentations generated from the grain was investigated. Four treatments were compared: three based on moist barley, either un-inoculated (M), inoculated with Wickerhamomyces anomalus (W), or inoculated with W. anomalus and LAB starter culture, containing Pediococcus acidilactici DSM 16243, Pediococcus pentosaceus DSM 12834 and Lactobacillus plantarum DSM 12837 (WLAB); and one treatment based on dried barley (D). After 6 weeks of storage, four feed fermentations FM, FW, FWLAB, and FD, were initiated from M, W, WLAB, and D, respectively, by mixing the grain with water to a dry matter content of 30%. Each treatment was fermented in batch initially for 7 days and then kept in a continuous mode by adding new feed daily with 50% back-slop. During the 6 week storage period, the average water activity decreased in M, W and WLAB from 0.96 to 0.85, and cereal pH decreased from approximately 6.0 at harvest to 4.5. Feed fermentation conferred a further pH decrease to 3.8-4.1. In M, W and WLAB, molds and Enterobacteriaceae were mostly below detection limit, whereas both organism groups were detected in D. In fermented feed, Enterobacteriaceae were below detection limit in almost all conditions. Molds were detected in FD, for most of the fermentation time in FM and at some sampling points in FW and FWLAB. Starter organisms, especially W. anomalus and L. plantarum comprised a considerable proportion of the yeast and LAB populations, respectively, in both stored grain and fermented feed. However, autochthonous Pichia kudriavzevii and Kazachstania exigua partially dominated the yeast populations in stored grain and fermented feed, respectively.

Grain storage loss is a major contributor to post-harvest losses and is one of the main causes of food insecurity for smallholder farmers in developing countries. Thus, the objective of this review is to assess the conventional and emerging grain storage practices for smallholder farmers in developing countries and highlight their most promising features and drawbacks. Smallholder farmers in developing countries use conventional grain storage structures and handling systems such as woven bags or cribs to store grain. However, they are ineffective against mold and insects already present in the grain before storage. Different chemicals are also mixed with grain to improve grain storability. Hermetic storage systems are effective alternatives for grain storage as they have minimal storage losses without using any chemicals. However, hermetic bags are prone to damage and hermetic metal silos are cost-prohibitive to most smallholder farmers in developing countries. Thus, an ideal grain storage system for smallholder farmers should be hermetically sealable, mechanically durable, and cost-effective compared to the conventional storage options. Such a storage system will help reduce grain storage losses, maintain grain quality and contribute to reducing food insecurity for smallholder farmers in developing countries.

The selective application of insecticides is one of the cornerstones of integrated pest management (IPM) and management strategies for pest resistance to insecticides. The present work provides a comprehensive overview of the traditional and new methods for the application of gas, liquid, gel, and solid physical insecticide formulations to control stored-product and food industry urban pests from the taxa Acarina, Blattodea, Coleoptera, Diptera, Hymenoptera, Lepidoptera, Psocoptera, and Zygentoma. Various definitions and concepts historically and currently used for various pesticide application formulations and methods are also described. This review demonstrates that new technological advances have sparked renewed research interest in the optimization of conventional methods such as insecticide aerosols, sprays, fumigants, and inert gases. Insect growth regulators/disruptors (IGRs/IGDs) are increasingly employed in baits, aerosols, residual treatments, and as spray-residual protectants for long-term stored-grain protection. Insecticide-impregnated hypoxic multilayer bags have been proven to be one of the most promising low-cost and safe methods for hermetic grain storage in developing countries. Insecticide-impregnated netting and food baits were originally developed for the control of urban/medical pests and have been recognized as an innovative technology for the protection of stored commodities. New biodegradable acaricide gel coatings and nets have been suggested for the protection of ham meat. Tablets and satchels represent a new approach for the application of botanicals. Many emerging technologies can be found in the form of impregnated protective packaging (insect growth regulators/disruptors (IGRs/IGDs), natural repellents), pheromone-based attracticides, electrostatic dust or sprays, nanoparticles, edible artificial sweeteners, hydrogels, inert baits with synthetic attractants, biodegradable encapsulations of active ingredients, and cyanogenic protective grain coatings. Smart pest control technologies based on RNA-based gene silencing compounds incorporated into food baits stand at the forefront of current strategic research. Inert gases and dust (diatomaceous earth) are positive examples of alternatives to synthetic pesticide products, for which methods of application and their integration with other methods have been proposed and implemented in practice. Although many promising laboratory studies have been conducted on the biological activity of natural botanical insecticides, published studies demonstrating their effective industrial field usage in grain stores and food production facilities are scarce. This review shows that the current problems associated with the application of some natural botanical insecticides (e.g., sorption, stability, field efficacy, and smell) to some extent echo problems that were frequently encountered and addressed almost 100 years ago during the transition from ancient to modern classical chemical pest control methods.

Bisphenol A (BPA) and polystyrene (PS) microplastics have attracted much attention because they are widely distributed in the environment, while their combined toxicity to aquatic organisms has rarely been studied. Therefore, this study explored the impact of microplastics on the toxic effects and biodegradation of BPA to the microalgae Chlorella pyrenoidosa. The results indicated that during the 16 days culture, PS (5 mg/L) increased the growth inhibition of BPA (1 mg/L and 10 mg/L) on C. pyrenoidosa compared to without PS. Similarly, PS (5 mg/L and 100 mg/L) also increased the degradation efficiency of BPA (1 mg/L and 10 mg/L) by algae. However, the changes of the chlorophyll content and the Fv/Fm value were opposite due to the hermetic and shading effect. Moreover, this study also found that five intermediates were formed during BPA degradation process because of the presence of oxidoreductase and glycosyltransferase. The results of the study provided vital information on the effect of PS on the toxicity and biodegradation of BPA to microalgal.

The objective of this study was to evaluate the impact of different 1-butanol vapor doses and its interaction with storage temperature on ‘Midori’ cabbage pigments, color changes and overall quality over two months of storage at 0.5 and 3 °C, plus 7 days shelf life at 20 °C. The cabbages were harvested and immediately treated with 1-butanol, as follows: (1) Control, chambers with samples were closed only; (2) 80 mg L −1 of 1-butanol; (3) 160 mg L −1 of 1-butanol; (4) 240 mg L −1 of 1-butanol; and (5) 320 mg L −1 of 1-butanol vapor. The cabbage heads were exposed to 1-butanol vapor during 48 h in 80 L hermetically sealed chambers. After treatment, cabbages were stored for two months at 0.5 and 3 °C. Subsequently, the heads were held for 7 days at 20 ºC, to simulate shelf life period, before pigments, color changes, and overall quality evaluations. The treatment with 1-butanol vapor resulted in little effect on quality at 0.5 °C. In contrast, at 3 °C, 1-butanol at 160 mg L −1 maintained overall quality during storage and after 7 days shelf life at 20 °C, preserving pigment, green color and overall quality. However, increasing the dose to 240 or 320 mg L −1 resulted in higher decay incidence and reduced quality. Conversely, without 1-butanol, storing at 0.5 °C preserved chlorophyll levels, green color, firmness, and minimized decay. So, it can be concluded that 160 mg L −1 1-butanol suppressed the pigment degradation and allows cabbage quality maintenance at a higher storage temperature (3 °C), being a promising technology to reduce losses of heads (decay and biochemical quality) at this storage temperature.

The performance of six grain storage technologies for the control of insect pests in maize was evaluated over a 36-week (9-month) storage period. The six technologies used were: two ZeroFly® hermetic bag brands (laminated and non-laminated); Purdue Improved Crop Storage (PICS) bag; non-hermetic ZeroFly® bag; woven polypropylene (PP) bag containing maize grain treated with Actellic Gold® Dust (pirimiphos-methyl 1.6% + thiamethoxam 0.3%) and woven PP bag containing untreated grain. Each bag was filled with 50 kg maize grain and four replicates of each were set up. With the exception of the non-hermetic ZeroFly® bag, 50 live adults of the larger grain borer Prostephanus truncatus and of the maize weevil Sitophilus zeamais, were introduced into all the bags. Insects were not introduced into the non-hermetic ZeroFly® bag to assess its effectiveness in repelling infestation from outside. Parameters recorded were gas composition (oxygen and carbon dioxide) levels inside the bags; weight of flour generated by insect feeding activities; grain moisture level; live adult insect counts; grain damage and weight loss; grain germination rate and aflatoxin level. At termination, the plastic liners of the hermetic bags were examined for perforations. Results show that oxygen depletion and carbon dioxide evolution were faster in ZeroFly® hermetic compared to PICS bags. Throughout the 36-week storage trial, grain damage remained below 4% and weight loss below 3% in all the treatments except in the untreated PP bags in which it increased to 81.1 and 25.5%, respectively. The hermetic PICS, ZeroFly® and Actellic Gold dust-treated PP bags maintained grain germination at 60%, which was lower than the initial 90%, while in untreated control, it reduced to 4.7%. The mean aflatoxin levels fluctuated between 0.39 and 3.56 parts per billion (ppb) during 24 weeks of storage in all the technologies tested, which is below the acceptable maximum level of 10 ppb in maize. Based on the evaluation results, it can be concluded that hermetic PICS and ZeroFly® bags and woven PP bag with Actellic Gold dust-treated grain effectively protected stored maize grain from insect attack and weight losses. Appropriate strategies and mechanisms for the effective and efficient adoption of hermetic storage bag technology at scale would contribute towards global food security.

Post-harvest storage of wheat in grain storage bags is commonly adopted practice in many developing countries, which offers a viable chemical-free approach to control storage insects. The damage potential of Tribolium castaneum in wheat grains stored in different storage bags at various storage periods was studied. A highly significant effect (P < 0.01) of the storage bags and storage periods was observed in variables measured (insect growth rate, damaged grains, grain weight loss and weight of insect feeding residues). The maximum mean insect growth rate (85.20 ± 2.80), percent damaged wheat grains (73.24 ± 0.17%), percent grain weight loss (21.14 ± 0.21%) and weight of insect feeding residues (2.15 ± 0.01 g) were recorded in wheat grains stored in cotton cloth bag at 150 d, whereas minimum mean insect growth rate (30.00 ± 0.00), percent damaged wheat grains (10.44 ± 0.16%), percent grain weight loss (0.82 ± 0.06%) and weight of insect feeding residues (0.22 ± 0.02 g) were recorded on wheat grains stored in hermetic bag due to 100% mortality of T. castaneum in hermetic bag at 30 d. A significant and positive correlation between storage periods, insect growth rate, damaged wheat grains, grain weight loss and weight of insect feeding residues was observed, except for the storage bags which were significantly and negatively correlated with other factors. In addition, the simple and linear regressions analysis were significant (P < 0.01), and all the equations for these analyses were fitted for the regression models and perfectly described the association between the dependent and independent variables. Hermetic bag proved resistance to external environment fluctuations in abiotic factors such as temperature and relative humidity. While grain moisture in all other bags fluctuated with external environment. The results showed that quantitative and qualitative losses of stored wheat grains depends not only the type of storage bags, but also on the storage periods. T. castaneum can have a negative impact on wheat grains stored in all storage bags except hermetic bag. So, it is recommended that hermetic bag be used at farmers’ level to achieve greater benefits by reducing T. castaneum infestation.

Na,K-ATPase is a member of the P-type ATPase family, which transforms the energy of ATP to the transmembrane Na/K gradient that is used to create membrane potential, support the excitability of neurons and myocytes, control pH, and transport substances. The regulation of the Na,K-ATPase function by physiological regulators also comprises a central role in the adaptation of organisms to different conditions. H2O2 is one of the main signaling molecules in redox metabolism and plays important function in cellular physiology. H2O2 also regulates signaling pathways via the specific oxidation of proteins harboring redox-sensitive moieties, like metal centers or cysteine residues, which control their activity. The Na,K-ATPase is redox-sensitive with an “optimal redox potential range,” where the reactive oxygen species (ROS), levels beyond this “optimal range” are responsible for enzyme inhibition. Thus reactive oxygen species manifest a hermetic effect, which is expressed by biphasic action; stimulation by low doses and inhibition by high doses. This study was aimed to reveal redox-sensitivity of brain synaptic membrane fractions Na,K-ATPase via H2O2 effects. Different concentrations of H2O2 change the kinetic parameters of the enzyme system for MgATP complex, Na+, and K+ differently. Moreover, H2O2 changes p-chloromercuribenzoic acids (PCMB) affinity. H2O2 targets thiols of the Na,K-ATPase – low and high concentrations of H2O2 change the oxidative state of thiolate (S‐) from Cys differently, resulting in the corresponding activation or inhibition of the enzyme. Targeting thiols of the Na,K-ATPase tunes the activity of the Na,K-ATPase to the cellular demands and sustains the enzyme activity at the “optimal” level.

During the last decade, post-harvest losses (PHL) reduction has been topping the agenda of governments as a pathway for addressing food security, poverty, and nutrition challenges in Africa. Using survey data from 579 households, we investigated the factors that affect farmers’ decisions to adopt post-harvest technologies: mechanized shelling, drying tarpaulins, and airtight storage validated for reducing PHL in Tanzania’s maize-based systems, and the impacts on households’ food security and welfare. Mechanized shelling addressed a labor issue, while tarpaulins and airtight storage addressed product quality and quantity concerns. The results revealed large farm sizes and location in higher production potential zones (proxies for higher production scale) and neighbors' use of the technologies as universal drivers for adoption. Access to credit and off-farm income were unique determinants for airtight storage, while group membership increased the probability of adopting drying tarpaulin and airtight storage. The technologies have positive impacts on food security and welfare: drying tarpaulins and airtight storage significantly increased food availability (18–27%), food access (24–26%), and household incomes (112–155%), whereas mechanized shelling improved food and total expenditures by 49% and 68%, respectively. The share of total household expenditure on food decreased by 42%, 11%, and 51% among tarpaulin, mechanized shelling, and airtight storage adopter households, signaling significant improvements in food security and reductions in vulnerability. The results point to the need for policy support to enhance the adoption of these technologies, knowledge sharing among farmers, and financial resources access to support investments in the technologies.