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Serendipitous findings and studies on Tuber species suggest that some ectomycorrhizal fungi, beyond their complex interaction with ectomycorrhizal hosts, also colonise roots of nonectomycorrhizal plants in a loose way called endophytism. Here, we investigate endophytism of T. melanosporum and T. aestivum . We visualised endophytic T. melanosporum hyphae by fluorescent in situ hybridisation on nonectomycorrhizal plants. For the two Tuber species, microsatellite genotyping investigated the endophytic presence of the individuals whose mating produced nearby ascocarps. We quantified the expression of four T. aestivum genes in roots of endophyted, non‐ectomycorrhizal plants. Tuber melanosporum hyphae colonised the apoplast of healthy roots, confirming endophytism. Endophytic Tuber melanosporum and T. aestivum contributed to nearby ascocarps, but only as maternal parents (forming the flesh). Paternal individuals (giving only genes found in meiotic spores of ascocarps) were not detected. Gene expression of T. aestivum in non‐ectomycorrhizal plants confirmed a living status. Tuber species, and likely other ectomycorrhizal fungi found in nonectomycorrhizal plant roots in this study, can be root endophytes. This is relevant for the ecology (brûlé formation) and commercial production of truffles. Evolutionarily speaking, endophytism may be an ancestral trait in some ectomycorrhizal fungi that evolved from root endophytes.

Morels ( Morchella spp.) are of great economic and scientific value. Paecilomyces penicillatus can cause white mold disease (WMD) widely emerging on morel ascocarps and is also a potential factor causing morel fructification failure. 1-octen-3-ol is a mushroom volatile compound with broad-spectrum antimicrobial activities. This study aimed to control the morel disease caused by P. penicillatus through suppressing P. penicillatus in the soil cultivated with Morchella sextelata using 1-octen-3-ol. Safe concentration of 1-octen-3-ol was estimated by comparing its inhibitory effect against P. penicillatus and M. sextelata , respectively, with mycelium-growth experiments on agar plates. The results showed that M. sextelata possesses a higher tolerance to 1-octen-3-ol than P. penicillatus with a 1-octen-3-ol concentration between 0 and 200 µL/L. Based on that, a sandy soil was supplemented with low (50 µL/L) or high concentration (200 µL/L) of 1-octen-3-ol. The effects of 1-octen-3-ol on soil microbial communities, WMD incidence, and morel yield were investigated. Compared to the non-supplemented control group, the incidence of WMD and the proportion of Paecilomyces in the soils of low- and high-concentration treatment groups were significantly decreased, corresponding to a significant increase in morel ascocarp yield. It suggests that 1-octen-3-ol effectively suppressed P. penicillatus in the soil, thereby reducing the severity of WMD and improving the morel yield. The diversity of soil bacterial communities was also altered by 1-octen-3-ol supplement. The proportion of Rhodococcus spp. in the soil was positively correlated with the 1-octen-3-ol concentration and ascocarp yield, suggesting its potential role in improving morel yield. Key points • A novel method for morel disease suppression was proposed. • Paecilomyces in soil affects white mold disease and fructification yield of morel. • 1-Octen-3-ol suppresses Paecilomyces and alters bacterial community in soil.

The primary purpose of the study, as part of the planned conservation work, was to uncover all aspects of autochthonous biofilm pertaining to the formation of numerous deterioration symptoms occurring on the limestone Rožanec Mithraeum monument in Slovenia. Using state-of-the-art sequencing technologies combining mycobiome data with observations made via numerous light and spectroscopic (FTIR and Raman) microscopy analyses pointed out to epilithic lichen Gyalecta jenensis and its photobiont, carotenoid-rich Trentepohlia aurea , as the origin of salmon-hued pigmented alterations of limestone surface. Furthermore, the development of the main deterioration symptom on the monument, i.e., biopitting, was instigated by the formation of typical endolithic thalli and ascomata of representative Verrucariaceae family ( Verrucaria sp.) in conjunction with the oxalic acid-mediated dissolution of limestone. The domination of lichenized fungi, as the main deterioration agents, both on the relief and surrounding limestone, was additionally supported by the high relative abundance of lichenized and symbiotroph groups in FUNGuild analysis. Obtained results not only upgraded knowledge of this frequently occurring but often overlooked group of extremophilic stone heritage deteriogens but also provided a necessary groundwork for the development of efficient biocontrol formulation applicable in situ for the preservation of similarly affected limestone monuments.

Utilising the rhizosphere microbiota as a biological control agent is a promising strategy to protect plants against pathogens, although its efficacy in fungal hosts is uncertain. This study investigated the efficacy of Pseudomonas chlororaphis, a bacterial strain, in mitigating Paecilomyces penicillatus, a soil-borne pathogenic fungus responsible for white mould disease (WMD) in cultivated morels, such as Morchella importuna. Soils with chronic WMD, inoculated with or without P. chlororaphis, were utilised for M. importuna cultivation. In P. chlororaphis-inoculated morel soil beds, P. chlororaphis colonised both the mycelial surface and ascocarp matrix of M. importuna, increasing the abundance of Morchella in soil and the alpha-diversity of the soil fungal community. Additionally, P. chlororaphis inoculation decreased the abundance of detrimental P. penicillatus and mitigated the WMD incidence, which correspondingly increased the morel yield. Metagenomics revealed that increasing the pseudomonads in the M. importuna mycosphere altered the functionalities of the M. importuna soil microbiota, enhancing the abundances of genes encoding chitinase and alkaline protease and reducing the abundances of genes encoding glucanase and laccase. Under P. chlororaphis inoculation, pathways associated with pathogenic invasion were under-represented in the soil microbiota. These results enhance our understanding of bacterial-fungal interactions within soil ecosystems and demonstrate the potential for disease suppression through microbiota manipulation within the fungal mycosphere. These insights may lead to innovative approaches to combat fungal pathogens and enhance the health and productivity of valuable fungal crops such as morels.

Truffles are ectomycorrhizal species forming edible ascocarps. The Italian white truffle (Tuber magnatum Pico) is the most famous and expensive species harvested to date; it comes exclusively from natural habitats in European countries. The annual production of T. magnatum is generally insufficient to respond to the high demands making its cultivation a research hotspot. The first attempt to cultivate T. magnatum started in the 1970s without success; only recently have mycorrhized plants been successfully produced. The aims of this study were (1) to assess the persistence of T. magnatum in the soil of plantations realized with mycorrhized plants and (2) to characterize the first T. magnatum orchard that produced ascocarps outside the known natural geographic range of this species. In 2018, five orchards were sampled in France, and T. magnatum was investigated in the soil. We confirmed that T. magnatum survived in the soil 3 to 8 years after planting. The key finding of this study was the harvest of T. magnatum ascocarps in 2019 and 2020 from one orchard. The production of ascocarps started 4.5 years after planting, and the ascocarps were harvested under different trees and during two consecutive seasons. A detailed analysis of the productive orchards (i.e., soil features, soil water availability, cultivation techniques) is presented. These results demonstrate the feasibility of T. magnatum cultivation worldwide by planting mycorrhized plants. The cultivation of T. magnatum could therefore become a real opportunity for farmers and could respond to the high demand of this high-priced food.

In this study, we examined the effect of the presence of mycorrhiza and ascomata of summer truffle (Tuber aestivum) on the bacterial composition of roots from small trees growing in selected sites of the Nida Basin. Qualitative DNA sequencing methods such as Sanger and next-generation sequencing (NGS) were used.The Sanger method revealed different bacterial species compositions between the samples where summer truffle ascomata was recorded and control samples. Five genera of bacteria could be distinguished: Bacillus, Erwinia, Pseudomonas, Rahnella and Serratia, among which the most numerous were Pseudomonas (Gammmaproteobacteria class) at 32.9%. The results obtained by the NGS method also showed differences in species composition of the bacteria depending on the study sample. Seven genera of bacteria were distinguished: Rhizorhabdus, Methylotenera, Sphingomonas, Nitrosospira, Streptomyces, Methyloceanibacter and Niastella, which dominated in roots from the truffle sites. Telmatobacter, Roseiarcus, Granulicella, Paludibaculum, Acidipila, Acidisphaera and Aliidongia dominated in roots from the control sites. With the NGS method, it is possible to identify the microbiome of a whole root, while only a root fragment can be analysed by the Sanger method.These results extend the scope of knowledge on the preferences of certain groups of bacteria associated with truffles and their influence on the formation of ascomata in summer truffles. Our results may also be useful in selecting and monitoring sites that promote ascomata of Tuber aestivum.

Fungi belonging to the genus Tuber produce edible ascocarps known as truffles. Tuber magnatum Picco may be the most appreciated truffle species given its peculiar aroma. While its life cycle is not yet fully elucidated, some studies demonstrated an active role of microorganisms. The main goal of this study was to determine how the T. magnatum microbiome varies across space and time. To address this, we characterized microbial communities associated with T. magnatum through high-throughput amplicon sequencing of internal transcribed spacer (ITS) and 16S rDNAs in three productive natural sites in Italy across 2 years. At each site, four truffles were sampled as well as the soil underneath and at 40, 100, and 200 cm from the harvesting points, to assess for microbial variation between substrates, years, and sites. A statistically significant site-related effect on microbial communities was identified, whereas only the prokaryotic community was significantly affected by the distance of soil from the truffle. Significant differences between sampling years were also found, demonstrating a possible relation among rainfall precipitation and Firmicutes and Actinobacteria . Thirty-six bacterial OTUs in truffles and 11 bacterial OTUs in soils beneath truffles were identified as indicator taxa. As shown for other truffle species, the dominance of Bradyrhizobium , Rhizobium , and Ensifer spp. within the truffle fruiting body suggests an evolutionary adaptation of this microorganism to the genus Tuber . The present work offers novel and relevant insights into the microbial ecology of T. magnatum ecosystems and fruiting bodies. The function and role of these bacteria in the truffle microbiome and life cycle are in need of further investigation.

Although the lifestyle of Geoglossales remains largely unknown, recent advancements have established a hypothesis regarding the ericoid mycorrhizal lifestyle of geoglossoid fungi. In this study, we focused on one isolate of Geoglossales sp. obtained from surface-sterilized roots of potted Rhododendron transiens. We aimed to reveal the phylogenetic position and in vitro colonizing ability of this species in the hair roots of ericoid mycorrhizal plants. Based on our multigene phylogenetic tree, this species is a sister of the genus Sarcoleotia which has not been reported from either other studies or field environment. Its ascocarps could not be obtained, and conspecific sequences were not found in the databases and repositories examined. The Geoglossales sp. colonized the vital rhizodermal cells of blueberries in vitro with hyphal coils. There were relatively large morphological variations of coils consistent with extraradical hyphae; however, overall, the colonization morphologically resembled those by Sarcoleotia globosa and representative ericoid mycorrhizal fungi. The taxonomy and ecological significance of the species remain to be resolved; nevertheless, our results suggest that the ericoid mycorrhizal lifestyle may be widespread within Geoglossales.

A revisionary synopsis is presented for the family Trypetheliaceae, based on a separately published phylogenetic analysis of a large number of species, morpho-anatomical and chemical study of extensive material, and revision of numerous type specimens. A total of 418 species is formally accepted in this synopsis, distributed among 15 genera as follows: Aptrootia (3), Architrypethelium (7), Astrothelium (242), Bathelium (16), Bogoriella (29), Constrictolumina (9), Dictyomeridium (7), Distothelia (3), Marcelaria (3), Nigrovothelium (2), Novomicrothelia (1), Polymeridium (50), Pseudopyrenula (20), Trypethelium (16), and Viridothelium (10). All accepted genera, including new genera described separately in this issue, are keyed out and briefly described and discussed, and keys are provided for all accepted species within each genus. Entries with full synonymy and brief descriptions, and in part also discussions, are provided for all accepted species, except those newly described elsewhere in this issue, which are cross-referenced in the corresponding keys. The description of the newly defined genera takes into account phylogeny in combination with morpho-anatomical features with the result that they are mostly recognizable by a combination of thallus, ascoma and ascospore features. Most species previously assigned to the genera Astrothelium, Campylothelium, Cryptothelium, and Trypethelium, based on a schematic concept of ascoma morphology and ascospore septation, are now included in a single genus, Astrothelium, with highly variable ascoma morphology and ascospore septation but invariably with astrothelioid ascospores (at least when young), that is diamond-shaped lumina, and a well-developed, corticate, usually olive-green thallus that often covers the ascomata. While the genera Aptrootia (large, brown, muriform ascospores), Architrypethelium (large, mostly 3-septate ascospores), and Pseudopyrenula (ecorticate, white thalli and astrothelioid ascospores) are maintained, Trypethelium is redefined to include species with raised, pseudostromatic ascomata and multiseptate ascospores with thin septa. The sister group of Trypethelium is the genus Marcelaria, with brightly coloured pseudostromata and muriform ascospores. Bathelium is now limited to species with strongly raised, fully exposed pseudostromata and septate to muriform ascospores with thin septa. Several genera are recognized for more basal lineages with mostly ecorticate, white thalli and solitary, exposed ascomata previously assigned to Arthopyrenia, Mycomicrothelia and Polymeridium, viz. Bogoriella, Constrictolumina, Dictyomeridium, and Novomicrothelia. In addition, separate genera are accepted for the Trypethelium tropicum (Nigrovothelium) and T. virens (Viridothelium) groups. In addition, a refined species concept resulting from phylogenetic studies is employed which pays particular attention to morphological features of the thallus and ascomata. Of a total of 526 names checked, 107 remain synonyms of accepted names and a further eight are newly excluded from the family. Based on these redispositions, the following 146 new combinations are proposed, including reinstatement of numerous names previously subsumed into synonymy: Architrypethelium columbianum (Nyl.) Aptroot & Lücking comb. nov., A. grande (Kremp.) Aptroot & Lücking comb. nov., Astrothelium aeneum (Eschw.) Aptroot & Lücking comb. nov., A. alboverrucum (Makhija & Patw.) Aptroot & Lücking comb. nov., A. amazonum (R. C. Harris) Aptroot & Lücking comb. nov., A. ambiguum (Malme) Aptroot & Lücking comb. nov., A. andamanicum (Makhija & Patw.) Aptroot comb. nov., A. annulare (Spreng.) Aptroot & Lücking comb. nov., A. aurantiacum (Makhija & Patw) Aptroot & Lücking comb. nov., A. auratum (R. C. Harris) Aptroot & Lücking comb. nov., A. aureomaculatum (Vain.) Aptroot & Lücking comb. nov., A. basilicum (Kremp.) Aptroot & Lücking comb. nov., A. bicolor (Taylor) Aptroot & Lücking comb. nov., A. buckii (R. C. Harris) Aptroot & Lücking comb. nov., A. calosporum (Müll. Arg.) Aptroot & Lücking comb. nov., A. cartilagineum (Fée) Aptroot & Lücking comb. nov., A. cecidiogenum (Aptroot & Lücking) Aptroot & Lücking comb. nov., A. ceratinum (Fée) Aptroot & Lücking comb. nov., A. chapadense (Malme) Aptroot & Lücking comb. nov., A. chrysoglyphum (Vain.) Aptroot & Lücking comb. nov., A. chrysostomum (Vain.) Aptroot & Lücking comb. nov., A. cinereorosellum (Kremp.) Aptroot & Lücking comb. nov., A. cinereum (Müll. Arg.) Aptroot & Lücking comb. et stat. nov., A. confluens (Müll. Arg.) Aptroot & Lücking comb. nov., A. consimile (Müll. Arg.) Aptroot & Lücking comb. nov., A. deforme (Fée) Aptroot & Lücking comb. nov., A. defossum (Müll. Arg.) Aptroot & Lücking comb. nov., A. degenerans (Vain.) Aptroot & Lücking comb. nov., A. dissimilum (Makhija & Patw.) Aptroot & Lücking comb. nov., A. effusum (Aptroot & Sipman) Aptroot & Lücking comb. nov., A. endochryseum (Vain.) Aptroot & Lücking comb. nov., A. exostemmatis (Müll. Arg.) Aptroot & Lücking comb. nov., A. feei (C. F. W. Meissn.) Aptroot & Lücking comb. nov., A. ferrugineum (Müll. Arg.) Aptroot & Lücking comb. nov., A. galligenum (Aptroot) Aptroot & Lücking comb. nov., A. gigantosporum (Müll. Arg.) Aptroot & Lücking comb. nov., A. indicum (Upreti & Ajay Singh) Aptroot & Lücking comb. nov., A. infossum (Nyl.) Aptroot & Lücking comb. nov., A. infuscatulum (Müll. Arg.) Aptroot & Lücking comb. nov., A. irregulare (Müll. Arg.) Aptroot & Lücking comb. nov., A. keralense (Upreti & Ajay Singh) Aptroot & Lücking comb. nov., A. kunzei (Fée) Aptroot & Lücking comb. nov., A. leioplacum (Müll. Arg.) Aptroot & Lücking comb. nov., A. lugescens (Nyl.) Aptroot & Lücking comb. nov., A. luridum (Zahlbr.) Aptroot & Lücking comb. nov., A. macrocarpum (Fée) Aptroot & Lücking comb. nov., A. macrosporum (Makhija & Patw.) Aptroot & Lücking comb. nov., A. marcidum (Fée) Aptroot & Lücking comb. nov., A. megaleium (Kremp.) Aptroot & Lücking comb. nov., A. megalophthalmum (Müll. Arg.) Aptroot & Lücking comb. nov., A. megalostomum (Vain.) Aptroot & Lücking comb. nov., A. megaspermum (Mont.) Aptroot & Lücking comb. nov., A. meiophorum (Nyl.) Aptroot & Lücking comb. nov., A. meristosporoides (P. M. McCarthy & Vongshew.) Aptroot & Lücking comb. nov., A. meristosporum (Mont. & Bosch) Aptroot & Lücking comb. nov., A. neogalbineum (R. C. Harris) Aptroot & Lücking comb. nov., A. nigratum (Müll. Arg.) Aptroot & Lücking comb. et stat. nov., A. nigrorufum (Makhija & Patw.) Aptroot & Lücking comb. nov., A. nitidiusculum (Nyl.) Aptroot & Lücking comb. nov., A. octosporum (Vain.) Aptroot & Lücking comb. nov., A. oligocarpum (Müll. Arg.) Aptroot & Lücking comb. nov., A. olivaceofuscum (Zenker) Aptroot & Lücking comb. nov., A. papillosum (P. M. McCarthy) Aptroot & Lücking comb. nov., A. papulosum (Nyl.) Aptroot & Lücking comb. nov., A. peranceps (Kremp.) Aptroot & Lücking comb. nov., A. phaeothelium (Nyl.) Aptroot & Lücking comb. nov., A. phlyctaenua (Fée) Aptroot & Lücking comb. nov., A. porosum (Ach.) Aptroot & Lücking comb. nov., A. praetervisum (Müll. Arg.) Aptroot & Lücking comb. nov., A. pseudoplatystomum (Makhija & Patw.) Aptroot & Lücking comb. nov., A. pseudovariatum (Upreti & Ajay Singh) Aptroot & Lücking comb. nov., A. puiggarii (Müll. Arg.) Aptroot & Lücking comb. nov., A. pulcherrimum (Fée) Aptroot & Lücking comb. nov., A. pupula (Ach.) Aptroot & Lücking comb. nov., A. purpurascens (Müll. Arg.) Aptroot & Lücking comb. nov., A. pustulatum (Vain.) Aptroot & Lücking comb. nov., A. rufescens (Müll. Arg.) Aptroot & Lücking comb. et stat. nov., A. sanguinarium (Malme) Aptroot & Lücking comb. nov., A. santessonii (Letr.-Gal.) Aptroot & Lücking comb. nov., A. saxicola (Malme) Aptroot & Lücking comb. nov., A. scoria (Fée) Aptroot & Lücking comb. nov., A. scorizum (Müll. Arg.) Aptroot & Lücking comb. nov., A. sierraleonense (C. W. Dodge) Aptroot & Lücking comb. nov., A. sikkimense (Makhija & Patw.) Aptroot & Lücking comb. nov., A. spectabile (Aptroot & Ferraro) Aptroot & Lücking comb. nov., A. sphaerioides (Mont.) Aptroot & Lücking comb. nov., A. stramineum (Malme) Aptroot & Lücking comb. nov., A. straminicolor (Nyl.) Aptroot & Lücking comb. nov., A. subcatervarium (Malme) Aptroot & Lücking comb. nov., A. subdiscretum (Nyl.) Aptroot & Lücking comb. nov., A. subdisjunctum (Müll. Arg.) Aptroot & Lücking comb. nov., A. subdissocians (Nyl. ex Vain.) Aptroot & Lücking comb. et stat. nov., A. superbum (Fr.) Aptroot & Lücking comb. nov., A. tenue (Aptroot) Aptroot & Lücking comb. nov., A. thelotremoides (Nyl.) Aptroot & Lücking comb. nov., A. trypethelizans (Nyl.) Aptroot & Lücking comb. nov., A. tuberculosum (Vain.) Aptroot & Lücking comb. nov., A. ubianense (Vain.) Aptroot & Lücking comb. nov., A. variatum (Nyl.) Aptroot & Lücking comb. nov., A. vezdae (Makhija & Patw.) Aptroot & Lücking comb. nov., Bathelium austroafricanum (Zahlbr.) Aptroot & Lücking comb. nov., B. nigroporum (Makhija & Patw.) Aptroot & Lücking comb. nov., Bogoriella alata (Groenh. ex Aptroot) Aptroot & Lücking comb. nov., B. annonacea (Müll. Arg.) Aptroot & Lücking comb. nov., B. apposita (Nyl.) Aptroot & Lücking comb. nov., B. captiosa (Kremp.) Aptroot & Lücking comb. nov., B. collospora (Vain.) Aptroot & Lücking comb. nov., B. confluens (Müll. Arg.) Aptroot & Lücking comb. nov., B. conothelena (Nyl.) Aptroot & Lücking comb. nov., B. decipiens (Müll. Arg.) Aptroot & Lücking comb. nov., B. exigua (Müll. Arg.) Aptroot & Lücking comb. nov., B. fumosula (Zahlbr.) Aptroot & Lücking comb. nov., B. hemisphaerica (Müll. Arg.) Aptroot & Lücking comb. nov., B. lateralis (Sipman) Aptroot & Lücking comb. nov., B. leuckertii (D. Hawksw. & J. C. David) Aptroot & Lücking comb. nov., B. macrocarpa (Komposch, Aptroot & Hafellner) Aptroot & Lücking comb. nov., B. megaspora (Aptroot & M. Cáceres) Aptroot & Lücking comb. nov., B. miculiformis (Nyl. ex Müll. Arg.) Aptroot & Lücking comb. nov., B. minutula (Zahlbr.) Aptroot & Lücking comb. nov., B. modesta (Müll. Arg.) Aptroo

The following seven new species of pyrenocarpous lichens are described from Monte Pascoal in Bahia (Brazil): Astrothelium citrisporum Aptroot, Oliveira-Junior & M.Cáceres, with thallus ochraceous, UV-negative, ascomata fused in hemispherical, concolorous pseudostromata, hamathecium not inspersed, and ascospores submuriform, 5 × 1–2-septate, 35–40 × 18–20 µm, citriform, both ends pointed; A. eustominspersum Aptroot & Oliveira-Junior, with thallus pale greyish olivaceous, UV-negative, ascomata fused, ostiole UV+ yellow, hamathecium inspersed, and ascospores 3-septate, 25–27 × 7–7.5 µm; A. flavogigasporum Aptroot, with thallus olivaceous, UV-negative, ascomata single, ostioles apical, hamathecium yellowish (K-negative) inspersed, and ascospores 4/ascus, hyaline, densely muriform, 240–260 × 33–38 µm, long-ellipsoid, without thickened central septum; A. medioincrassatum Aptroot & M.Cáceres, with thallus olivaceous, UV-negative, ascomata fused in inconspicuous groups, ostioles lateral, hamathecium not inspersed, and ascospores 9–11-septate, 98–115 × 23–27 µm, long-ellipsoid, with thickened central septum; Pseudopyrenula gelatinosa Aptroot, with thallus UV-negative, ascomata solitary, ostioles apical, hamathecium not inspersed, and ascospores 3-septate, 34–37 × 9–10.5 µm, wall 1 µm thick, surrounded by a 9–10.5 µm thick gelatinous sheath; Pyrenula salmonea Aptroot, with thallus salmon pink, ascomata solitary, ostioles apical, hamathecium densely hyaline inspersed, and ascospores 3-septate, uniseriate, 24–27 × 13–16 µm, ellipsoid, lumina oval to somewhat angular, broader than long, without endospore between the outer lumina and the ascospore wall; and P. sanguineoastroidea Aptroot with thallus olivaceous, UV-negative, ascomata fused, deeply immersed in the bark, ostioles lateral, hamathecium not inspersed, and ascospores 3-septate, 24–27 × 10–12 µm, long-ellipsoid, lumina rhomboid, with thick endospore layer between the outer lumina and the ascospore wall. A further 353 species are reported, of which 12 are first records for Brazil and 192 are first records for the state of Bahia, despite it being one of the states of Brazil that is best investigated lichenologically. A graph is presented with the cumulative number of species collected after a certain time of fieldwork. It does not significantly level off, suggesting that many more species occur in the area. A key to the Pyrenula species known from Brazil is presented.