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This paper presents the development of an add-on for CAD system generating assembly structure automatically based on a given Bill of Materials (BOM). The BOM in Excel format is scanned by the system and the system arrange 3D CAD files into assembly at in CATIA V5 CAD system automatically. This program has been tested and succeeded well at compiling 3D assembly based on 3D part file data that is available or not available. If the 3D file is not available then the 3D assembly is still generated according to the assembly structure listed on Excel file but its 3D representation does not appear.

No detailed description available for \"Mexican Women in American Factories\".

The 4th industrial revolution (Industry 4.0, I4.0) is based upon the penetration of many new technologies to the industrial world. These technologies are posed to fundamentally change assembly lines around the world. Assembly systems transformed by I4.0 technology integration are referred to here as Assembly 4.0 (A4.0). While most I4.0 new technologies are known, and their integration into shop floors is ongoing or imminent, there is a gap between this knowledge and understanding the form and the impact of their full implementation in assembly systems. The path from the new technological abilities to improved productivity and profitability has not been well understood and has some missing parts. This paper strives to close a significant part of this gap by creating a road map to understand and explore the impact of typical I4.0 new technologies on A4.0 systems. In particular, the paper explores three impact levels: strategic, tactical, and operational. On the strategic level, we explore aspects related to the design of the product, process, and the assembly system. Additionally, the paper elaborates on likely changes in assembly design aspects, due to the flexibility and capabilities that these new technologies will bring. Strategic design also deals with planning and realizing the potential of interactions between sub-assembly lines, kitting lines, and the main assembly lines. On the tactical level, we explore the impact of policies and methodologies in planning assembly lines. Finally, on the operational level, we explore how these new capabilities may affect part routing and scheduling including cases of disruptions and machine failures. We qualitatively assess the impact on performance in terms of overall flow time and ability to handle a wide variety of end products. We point out the cases where clear performance improvement is expected due to the integration of the new technologies. We conclude by identifying research opportunities and challenges for advanced assembly systems.

The study of ecological networks has progressively evolved from a mostly descriptive science to one that attempts to elucidate the processes governing the emerging structure of multitrophic communities. To move forward, we propose a conceptual framework using trait-based inference of ecological processes to improve our understanding of network assembly and our ability to predict network reassembly amid global change. The framework formalizes the view that network assembly is governed by processes shaping the composition of resource and consumer communities within trophic levels and those dictating species’ interactions between trophic levels. To illustrate the framework and show its applicability, we (1) use simulations to explore network structures emerging from the interactions of these assembly processes, (2) develop a null model approach to infer the processes underlying network assembly from observational data, and (3) use the null model approach to quantify the relative influence of bottom-up (resource-driven) and top-down (consumer-driven) assembly modes on plant–frugivore networks along an elevational gradient. Simulations suggest that assembly processes governing the formation of pairwise interactions have a greater influence on network structure than those governing the composition of communities within trophic levels. Our case study further shows that the mode of network assembly along the gradient is mainly bottom-up controlled, suggesting that the filtering of plant traits has a larger effect on network structure relative to the filtering of frugivore traits. Combined with increasingly available trait and interaction data, the framework provides a timely toolbox to infer assembly processes operating within and between trophic levels and to test competing hypotheses about the assembly mode of resource–consumer networks along environmental gradients and among biogeographic regions. It is a step toward a more process-based network ecology and complete integration of multitrophic interactions in the prediction of future biodiversity

• High-efficiency methods for DNA assembly have enabled the routine assembly of synthetic DNAs of increased size and complexity. However, these techniques require customization, elaborate vector sets or serial manipulations for the different stages of assembly. • We have developed Loop assembly based on a recursive approach to DNA fabrication. The system makes use of two Type IIS restriction endonucleases and corresponding vector sets for efficient and parallel assembly of large DNA circuits. Standardized level 0 parts can be assembled into circuits containing 1, 4, 16 or more genes by looping between the two vector sets. • The vectors also contain modular sites for hybrid assembly using sequence overlap methods. - Loop assembly enables efficient and versatile DNA fabrication for plant transformation. We show the construction of plasmids up to 16 genes and 38 kb with high efficiency (> 80%). We have characterized Loop assembly on over 200 different DNA constructs and validated the fidelity of the method by high-throughput Illumina plasmid sequencing. • Our method provides a simple generalized solution for DNA construction with standardized parts. The cloning system is provided under an OpenMTA license for unrestricted sharing and open access.

Long-read de novo genome assembly continues to advance rapidly. However, there is a lack of effective tools to accurately evaluate the assembly results, especially for structural errors. We present Inspector, a reference-free long-read de novo assembly evaluator which faithfully reports types of errors and their precise locations. Notably, Inspector can correct the assembly errors based on consensus sequences derived from raw reads covering erroneous regions. Based on in silico and long-read assembly results from multiple long-read data and assemblers, we demonstrate that in addition to providing generic metrics, Inspector can accurately identify both large-scale and small-scale assembly errors.

Summary Brassica rapa comprises many important cultivated vegetables and oil crops. However, Chiifu v3.0, the current B. rapa reference genome, still contains hundreds of gaps. Here, we presented a near‐complete genome assembly of B. rapa Chiifu v4.0, which was 424.59 Mb with only two gaps, using Oxford Nanopore Technology (ONT) ultralong‐read sequencing and Hi‐C technologies. The new assembly contains 12 contigs, with a contig N50 of 38.26 Mb. Eight of the ten chromosomes were entirely reconstructed in a single contig from telomere to telomere. We found that the centromeres were mainly invaded by ALE and CRM long terminal repeats (LTRs). Moreover, there is a high divergence of centromere length and sequence among B. rapa genomes. We further found that centromeres are enriched for Copia invaded at 0.14 MYA on average, while pericentromeres are enriched for Gypsy LTRs invaded at 0.51 MYA on average. These results indicated the different invasion mechanisms of LTRs between the two structures. In addition, a novel repetitive sequence PCR630 was identified in the pericentromeres of B. rapa. Overall, the near‐complete genome assembly, B. rapa Chiifu v4.0, offers valuable tools for genomic and genetic studies of Brassica species and provides new insights into the evolution of centromeres.

Polymerization-induced self-assembly (PISA) is a powerful and versatile technique for producing colloidal dispersions of block copolymer particles with desired morphologies. Currently, PISA can be carried out in various media, over a wide range of temperatures, and using different mechanisms. This method enables the production of biodegradable objects and particles with various functionalities and stimuli sensitivity. Consequently, PISA offers a broad spectrum of potential commercial applications. The aim of this review is to provide an overview of the current state of rational synthesis of block copolymer particles with diverse morphologies using various PISA techniques and mechanisms. The discussion begins with an examination of the main thermodynamic, kinetic, and structural aspects of block copolymer micellization, followed by an exploration of the key principles of PISA in the formation of gradient and block copolymers. The review also delves into the main mechanisms of PISA implementation and the principles governing particle morphology. Finally, the potential future developments in PISA are considered.

Key Points The dynamic regulation of chromatin involves four subfamilies of ATP-dependent nucleosome-remodelling complexes: imitation switch (ISWI), chromodomain helicase DNA-binding (CHD), switch/sucrose non-fermentable (SWI/SNF) and INO80. Each subfamily is specialized to preferentially achieve particular chromatin outcomes: assembly, access or editing. Diversity in the protein composition of remodellers enables their specific interaction with particular transcription activators, repressors and histone modifications, which together specify targeting. Although diverse in protein composition, all remodellers have a similar ATPase 'motor' that translocates DNA from a common location within the nucleosome, which breaks histone–DNA contacts. The diverse specialized proteins and domains in each remodeller subfamily are also involved in detecting nucleosome epitopes, which differentially regulate the conserved ATPase–translocase motor to achieve the various chromatin-remodelling outcomes. We propose an 'hourglass' model of chromatin remodelling that involves convergence on a DNA translocation mechanism, which is preceded and followed by remodeller diversity, in terms of differential remodeller targeting and remodelling outcomes, respectively. Remodellers are emerging as 'smart' machines that are informed about whether or how to utilize DNA translocation to conduct chromatin remodelling. Nucleosome-remodelling complexes can slide or eject histones, or incorporate histone variants, but they share an ATPase–translocase 'motor' and a common DNA translocation mechanism. In a unifying 'hourglass' model of remodeller function, the different remodeller subfamilies use different modules for targeting to nucleosomes but converge on a DNA translocation mechanism and then diverge again to achieve various outcomes. Cells utilize diverse ATP-dependent nucleosome-remodelling complexes to carry out histone sliding, ejection or the incorporation of histone variants, suggesting that different mechanisms of action are used by the various chromatin-remodelling complex subfamilies. However, all chromatin-remodelling complex subfamilies contain an ATPase–translocase 'motor' that translocates DNA from a common location within the nucleosome. In this Review, we discuss (and illustrate with animations) an alternative, unifying mechanism of chromatin remodelling, which is based on the regulation of DNA translocation. We propose the 'hourglass' model of remodeller function, in which each remodeller subfamily utilizes diverse specialized proteins and protein domains to assist in nucleosome targeting or to differentially detect nucleosome epitopes. These modules converge to regulate a common DNA translocation mechanism, to inform the conserved ATPase 'motor' on whether and how to apply DNA translocation, which together achieve the various outcomes of chromatin remodelling: nucleosome assembly, chromatin access and nucleosome editing.

The history of themaquiladorashas been punctuated by workers' organized resistance to abysmal working and living conditions. Over years of involvement in such movements, Rosemary Hennessy was struck by an elusive but significant feature of these struggles: the extent to which organizing is driven by attachments of affection and antagonism, belief, betrayal, and identification. What precisely is the \"affective\" dimension of organizing for justice? Are affects and emotions the same? And how can their value be calculated?Fires on the Bordertakes up these questions of labor and community organizing-its \"affect-culture\"-on Mexico's northern border from the early 1970s to the present day. Through these campaigns, Hennessy illuminates the attachments and identifications that motivate people to act on behalf of one another and that bind them to a common cause. The book's unsettling, even jarring, narratives bring together empirical and ethnographic accounts-of specific campaigns, the untold stories of gay and lesbian organizers, love and utopian longing-in concert with materialist theories of affect and the critical good sense of Mexican organizers. Teasing out the integration of affect-culture in economic relations and cultural processes, Hennessy provides evidence that sexuality and gender as strong affect attractors are incorporated in the harvesting of surplus labor. At the same time, workers' testimonies confirm that the capacities for bonding and affective attachment, far from being entirely at the service of capital, are at the very heart of social movements devoted to sustaining life.