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Targeted profiling of polyphenols in trees may reveal valuable sources of natural compounds with major applications in pharmacology and disease control. The current study targeted the profiling of polyphenols using HPLC-DAD in Quercus robur, Q. macrocarpa and Q. acutissima bark extracts. Free radical scavenging of each extract was investigated using antioxidant assays. Antimicrobial activities against a wide spectrum of bacteria and fungi were explored, as well as anticancer activities against different cancer cell lines. The HPLC-DAD analyses revealed the availability of several polyphenols in high amounts, including ellagic acid (in Q. robur) and caffeic acid (in Q. macrocarpa) in all three species. The bioactivity assay revealed high antioxidant activity in Q. robur compared to that of the other species, as well as phenolic standards. The three oak bark extracts showed clear antibacterial activities against most bacteria tested, with the highest antibacterial activities in the extracts of Q. robur. In addition, the three extracts showed higher antibacterial activities against Pseudomonas aeruginosa, Micrococcus flavus, and Escherichia coli compared to that of other bacteria. There were strong antifungal activities against some fungi, such as Aspergillus flavus, Penicillium funiculosum, and Penicillium ochrochloron. There were also noticeable anticancer activities against MCF-7, HeLa, Jurkat, and HT-29 cell lines, with the highest anticancer activity in the extracts of Q. robur. This is the first study that reveals not only novel sources of important polyphenols (e.g., ellagic acid) in Q. robur, Q. macrocarpa and Q. acutissima bark but also their anticancer activities against diverse cancer cell lines.

Key messageThe leaf and bark extract of Trema orientalis has antioxidant and antibacterial properties. Its bark contains considerable amount of tannin and showed potential for leather tanning. The wood of this species is an excellent source of pulp production.Trema orientalis L. is a fast growing tree in tropic and sub-tropic regions whose leaf, bark and root extract has traditionally been used in therapeutic purposes in different parts of the world. Along with having antioxidant and antibacterial activities within the different plant tissue extracts, it also contains 22% tannin material that is suitable for leather tanning as well as biobased resin preparation. Wood from T. orientalis had good amount of α-cellulose (45–50%) and moderate amount of lignin (20–25%) leading to much research on T. orientalis pulping, where pulp yield in the kraft and soda-anthranquinone pulping processes was about 50% with kappa number around 18-25 that made it comparable to tropical hardwood pulp. Dissolving grade pulp with high purity could also be produced from this species which makes it a good alternative for pulpwood production in tropical countries. This review addresses the potential of complete utilization of the T. orientalis plant in biobased product development.

In recent years, phytofunctionalized AgNPs have attracted great interest due to their remarkable biological activities. In the present study, AgNPs were synthesized using Abies alba and Pinus sylvestris bark extracts. The chemical profile of these bark extracts was analyzed by LC-HRMS/MS. As a first step, the synthesis parameters (pH, AgNO3 concentration, ratio of bark extract and AgNO3, temperature, and reaction time) were optimized. The synthesized AgNPs were characterized by ATR-FTIR spectroscopy, DLS, SEM, EDX, and TEM. Their antioxidant, cytotoxic, and antibacterial properties were evaluated by the DPPH, ABTS, MTT, and broth microdilution assays, respectively. Abies alba and Pinus sylvestris bark extract-derived AgNPs were well-dispersed, spherical, small (average particle size of 9.92 and 24.49 nm, respectively), stable (zeta potential values of −10.9 and −10.8 mV, respectively), and cytotoxic to A-375 human malignant melanoma cells (IC50 = 2.40 ± 0.21 and 6.02 ± 0.61 μg/mL, respectively). The phytosynthesized AgNPs also showed antioxidant and antibacterial effects.

The aim of the study was to compare the effect of adding different sources of hydrolysable tannins to the sheep diet on protozoa population and carbohydrate digestion in the rumen. The study was performed in 3 Polish Lowland ewes fistulated to the rumen in a 3 × 3 Latin square design. Control sheep (CON) received (g/d): meadow hay (600), barley meal (300), soybean meal (100) and vitamin-mineral premix (20). Sheep from the experimental groups were additionally administered 12.6 g/kg DM oak bark extract (OAK) and 3.91 g/kg DM tannic acid (TAN ). The net consumption of tannins was approx. 0.4% DM for both additives. Regarding the count of protozoa, a significant interaction between diet and sampling time was documented for all ciliates (P<0.01), with a significant effect of both factors when considered separately. Experimental diets reduced the number of total protozoa and spp. (before feeding, 2 and 4 h after feeding; P<0.01), while increasing the abundance of spp. population (4 h after feeding; P<0.01) in the rumen. Interestingly, the count of spp. after feeding the TAN diet increased before feeding and 2 h after feeding in comparison to the CON and OAK groups, respectively, and subsequently decreased compared to the CON diet (4 and 8 h after feeding, P<0.01). A significant interaction between the diet and sampling time was observed for xylanolytic activity (P<0.01) in the rumen, with a significant effect of sampling time, which decreased its activity in CON (after feeding) and OAK sheep (2 h after feeding; P<0.01). For amylolytic activity (P<0.10), there was a trend towards a significant interaction between experimental factors, with a significant effect on both diet and sampling time. Detailed analysis showed that the TAN diet significantly reduced amylolytic activity 2 h after feeding compared to the CON group (P<0.05). In conclusion, the TAN diet significantly reduced the number of total protozoa and spp., which consequently reduced amylolytic activity in the rumen, without any significant effect on pH and carbohydrate fermentation in the rumen.

This current work revealed a single-step fabrication of tungsten oxide nanoflakes (WO3 NFs) with the help of Terminalia arjuna bark extract. Bioactive phytoconstituents of T. arjuna bark extract were involved in the nucleation process and promoted the material crystalline growth in a particular direction. The as-prepared sample thermal decomposition was analyzed by TG/DTG. The as-prepared sample was annealed at 300 °C for 2 h, and the annealed sample was characterized by UV-Vis-DRS, FTIR, Raman, XRD, SEM, EDX, and TEM. Synthesized WO3 samples showed a monoclinic phase of the flake-like structure with lengths of 25~230 nm and diameters of 25~120 nm. The WO3 NFs were evaluated against S. aureus and E. coli. Over 3 mg concentrations of WO3 NFs outperform the positive control in antibacterial activity. The pseudo-first-order kinetics of the WO3 NFs enhanced the photocatalytic performance of methylene blue (MB). These results prove that WO3 NFs have sustainable performance in antibacterial and MB degradation applications.

Background Excessive ultraviolet exposure triggers skin pigment deposition, potentially leading to conditions such as melasma, freckles, and post‐inflammatory hyperpigmentation. The skin of individuals with these dermatological conditions exhibits reduced levels of nicotinamide nucleotide transhydrogenase (NNT). While many seek skin‐whitening products to address pigmentation, existing options often fail to achieve optimal efficacy. To accomplish a synergistic whitening effect, we developed PPV, a composite formulation incorporating three depigmenting agents: Pterocarpus marsupium bark extract (PMBE), Pinus strobus bark extract (PSBE), and ascorbyl tetraisopalmitate (VC‐IP). Aims This study aimed to investigate the effects of PPV on skin whitening and elucidate its mechanisms. Methods After administering PPV to B16F10 melanoma cells, we measured melanin content and gene expression of TYR, TRP‐1, and TRP‐2 using a microplate reader and qPCR. Western blotting was used to assess p‐CREB, CREB, MITF, and NNT expression. The Jin's formula was used to evaluate the combined effect of the three components of PPV on NNT at the genetic level. Oxidative stress levels were evaluated with ROS, GSH/GSSG, and NADP+/NADPH assay kits, along with confocal microscopy. Transmission electron microscopy and ELISA were employed to quantify the eumelanin/pheomelanin ratio and melanosome maturation. Finally, immunodetection was performed to assess epidermal basal pigmentation, tyrosinase, and NNT expression in a human ex vivo skin model. Results PPV significantly influenced melanin production in melanocytes and human ex vivo skin. This effect was due to the inhibitory effect of PPV on the CREB‐MITF signaling pathway and the synergistic activation effect of PMBE, PSBE, and VC‐IP on the NNT gene expression in B16F10 cells. Activation of NNT led to a reduction in ROS levels and the NADP+/NADPH ratio, ultimately inhibiting melanogenesis, tyrosinase activity, and melanosome maturation in B16F10 cells. Furthermore, PPV treatment reduced pigmentation and increased NNT expression in the aged ex vivo skin model. Conclusion PPV, synthesized from PMBE, PSBE, and VC‐IP, exhibited an anti‐melanogenic effect. This effect was attributed to the inhibition of CREB‐MITF‐mediated melanogenesis and the regulation of oxidative stress by NNT. The findings suggest PPV as a potential therapeutic agent for skin pigmentation disorders.

Birch outer bark extract (BBE), containing pentacyclic triterpenes such as betulin, lupeol, and betulinic acid, is a widely recognized natural product renowned for its diverse pharmacological effects. However, its limited water solubility restricts its bioavailability. Therefore, the main objective is to enhance the bioavailability of BBE for pharmaceutical use. In this study, we aimed to develop a dispersion system utilizing a unique oleogel-producing method through the recrystallization of BBE from an ethanol solution in the oil phase. We generated an oleogel that demonstrates a notable 42–80-fold improvement in betulin and lupeol peroral bioavailability from BBE in Wistar rats, respectively. A physical paste-like BBE hydrogel developed with antisolvent precipitation showed a 16–56-fold increase in the bioavailability of betulin and lupeol from BBE in rat blood plasma, respectively. We also observed that the repeated administration of the BBE oleogel did not exhibit any toxicity at the tested dose (38.5 mg/kg betulin, 5.2 mg/kg lupeol, 1.5 mg/kg betulinic acid daily for 7 days). Betulin and betulinic acid were not detected in rat heart, liver, kidney, or brain tissues after the peroral administration of the oleogel daily for 7 days. Lupeol was found in rat heart, liver, and kidney tissues.

An acute, placebo-controlled, single-blind, crossover, dose-response, exploratory study was designed to investigate the hypoglycaemic effects of New Zealand pine bark extract (Enzogenol®). Twenty-five healthy participants categorised into having a monophasic or complex (biphasic or triphasic) glucose curve shape at the control visit consumed a placebo and Enzogenol® (50 and 400 mg) on three separate occasions before an oral glucose tolerance test (OGTT). In the monophasic group, 50 and 400 mg of Enzogenol® significantly reduced the mean glucose incremental area under the curve (iAUC) compared to control 241.3 ± 20.2 vs. 335.4 ± 34.0 mmol/L·min, p = 0.034 and 249.3 ± 25.4 vs. 353.6 ± 31.5 mmol/L·min, p = 0.012, respectively. The 400 mg dose further reduced the percentage increment of postprandial glucose (%PG) 31.4% ± 7.9% vs. 47.5% ± 8.6%, p = 0.010, glucose peak 7.9 ± 0.3 vs. 8.9 ± 0.3 mmol/L, p = 0.025 and 2h-OGTT postprandial glucose (2hPG) 6.1 ± 0.3 vs. 6.7 ± 0.3 mmol/L, p = 0.027. Glucose iAUC was not significantly different in the complex group, except for reductions in %PG 28.7% ± 8.2% vs. 43.4% ± 5.9%, p = 0.012 after 50 mg dose and 27.7% ± 5.4% vs. 47.3% ± 7.2%, p = 0.025 after 400 mg dose. The results suggest that Enzogenol® may have hypoglycaemic effects in healthy participants, especially those exhibiting monophasic shapes.

The aim of the study was to compare two sources of tannins on fatty acids (FA) composition in rumen. Treatments were (g tannins/kg diet as-feed-basis) as follows: (1) no supplemental tannin addition (CON), (2) addition of 13 g of oak bark extract (OAK), and (3) 4 g of tannic acid (TAN). The basal diet contained 55:45 forage to concentrate ratio. Net consumption of tannins (g/d) was 4 g for both tannins sources. The study was performed on three Polish Mountain ewes fitted with rumen cannulas, and was divided into three experimental periods (I, II, and III). Both sampling time and animal diet had a significant effect on FA profile in the rumen fluid. In general, FA concentrations were higher before feeding in comparison to samples collected 2 and 4 h after feeding. In terms of dietary effect, it was shown that TAN addition had a greater influence on FA profile in the ruminal fluid than the OAK diet. Briefly, in the TAN group significantly increased concentrations of C18:2 c9c12 (linoleic acid, LA) 8 h after feeding (vs. control, CON and OAK), C18:3 c9c12c15 (α-linolenic acid, LNA) 4 h after feeding (vs. OAK), C20:3 n-6 before feeding (vs. CON), C20:4 before feeding (vs. CON and OAK) and 8 h after feeding (vs. OAK) were recorded. In contrast, OAK addition significantly reduced C20:3 n-6 concentration 2 h after feeding (vs. CON). In conclusion, increased concentrations of both LA and LNA in the rumen indicated that supplemental tannic acid may inhibit the initial stage of FA biohydrogenation in the rumen.

Background Cervical cancer remains one of the significant causes of mortality in women due to the limitations of current treatment strategies and their associated side effects. Investigation of alternative medicine, including phytomedicine, has shown effective anti-cancer potential with fewer side effects. Azadirachta indica (commonly known as neem) is known for its medicinal properties. The present study investigated the anti-cancer potential of methanolic neem stem bark extract (MNBE) against cervical cancer using HeLa, SiHa, and ME-180 cell lines. Methods Cytotoxic effect of MNBE on cultured cell lines was evaluated by MTT and clonogenic assay. The growth-inhibiting effect of MNBE was further confirmed by performing cell cycle analysis and apoptosis assay using flow cytometry. The anti-migratory effect of MNBE was evaluated by using wound healing and Boyden chamber assay. Real-time PCR was used to determine the mRNA expression, and western blot and flow cytometry was used to determine the protein levels of growth and migration-related genes. Results MNBE significantly suppressed the growth and survival of cervical cancer cells in a dose-dependent manner by inducing cell cycle arrest and apoptosis. In addition, the growth inhibitory effect of MNBE was specific to cervical cancer cells than normal cells. Cell cycle arrest was correlated to transcriptional downregulation of cyclin dependent kinase 1 (CDK1), cyclin A, and cyclin B. Additionally, MNBE treatment resulted in the upregulation of active caspase-3 protein and downregulation of prosurvival genes, Bcl2, and survivin at mRNA level and NFkB-p65 at the protein level. Furthermore, MNBE inhibited the migration of cervical cancer cells accompanied by modulation of migration-related genes, including zona occludens-1 (ZO-1), matrix metalloproteinase 2 (MMP2), focal adhesion kinase (FAK), N-cadherin, snail, and E-cadherin. Conclusion In summary, the present study provides the first evidence of MNBE in restricting cervical cancer cell growth and migration, which warrants further investigation for developing novel anti-cancer drugs.