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The emergence of monkeypox has become a global health threat after the COVID‐19 pandemic. Due to the lack of available specifically treatment against MPV, developing an available vaccine is thus the most prospective and urgent strategy. Herein, a programmable macrophage vesicle based bionic self‐adjuvanting vaccine (AM@AEvs‐PB) is first developed for defending against monkeypox virus (MPV). Based on MPV‐related antigen‐stimulated macrophage‐derived vesicles, the nanovaccine is constructed by loading the mature virion (MV)‐related intracellular protein (A29L/M1R) and simultaneously modifying with the enveloped virion (EV) antigen (B6R), enabling them to effectively promote antigen presentation and enhance adaptive immune through self‐adjuvant strategy. Owing to the synergistic properties of bionic vaccine coloaded MV and EV protein in defensing MPV, the activation ratio of antigen‐presenting cells is nearly four times than that of single antigen in the same dose, resulting in stronger immunity in host. Notably, intramuscular injection uptake of AM@AEvs‐PB demonstrated vigorous immune‐protective effects in the mouse challenge attempt, offering a promising strategy for pre‐clinical monkeypox vaccine development. The monkeypox‐specific bionic vaccine (AM@AEvs‐PB) is consists of IMV antigens (A29L, M1R), the EEV antigen (B6R), and MPV‐preactivated macrophagederived vesicles. AM@AEvs‐PB can induce enhanced innate immune responses, promote cross‐presentation of antigens to dendritic cells (DCs), and elicit robust adaptive immune responses, realizing immunization protection against Monkeypox Virus.

The erythrocyte membranes used in nanovaccines include high membrane stability, long circulation life, adaptability and extremely good bio compatibility. Nanoparticles encapsulated by erythrocyte membranes are widely used as ideal drug delivery vehicles because of their high drug loading, long circulation time, and excellent biocompatibility. The mannose modification of delivery materials can help target mannose receptors (MRs) to deliver antigens to antigen-presenting cells (APCs). In this study, the antigen gene gp90 of avian reticuloendotheliosis virus (REV) was encapsulated with carboxymethyl chitosan (CS) to obtain CSgp90 nanoparticles, which were coated with mannose-modied fowl erythrocyte membranes to yield CS-gp90@M-M nanoparticles. The physicochemical characterization and immune response of the CS-gp90@M-M nanoparticles were investigated and . CS-gp90@M-M nanoparticles were rapidly phagocytized in vitro by macrophages to induce the production of cytokines and nitric oxide. In vivo, CS-gp90@M-M nanoparticles increased cytokine levels, the CD4+/8+ ratio, REV-specific antibodies in the peripheral blood of chicks, and the mRNA levels of immune-related genes in the spleen and bursa of immunized chicks. CS-gp90@M-M nanoparticles could be targeted to lymphoid organs to prolong the retention time of the nanoparticles at the injection site and lymphatic organs, leading to a strong, sustained immune response. Moreover, the CS-gp90@M-M nano-vaccine showed a lasting immunoprotective effect and improved the body weight of chicks after the challenge. Overall, CS-gp90@M-M nanoparticles can be used in vaccine designs as an effective delivery carrier with immune response-enhancing effects.

This paper focuses on the formidable challenges that underwater robots encounter in complex marine environments. To address these issues, inspired by the cownose ray, an innovative scheme is proposed, utilizing four six-bar mechanisms to mimic its pectoral fin movement. Subsequently, the paper elaborates on the design, computation, and simulation of the bionic pectoral fin mechanism. A Watt-type six-bar mechanism is adopted, and by axially overlaying two scaled-identical mechanisms and setting a phase difference, the pectoral fin waving of the cownose rays is simulated. SolidWorks and ADAMS are employed for precise modeling and simulation. Following this, an experimental prototype is constructed, with the rod assembly produced by subtractive machining. Motion capture and six-dimensional force experiments are then conducted to evaluate its motion dynamics and propulsion efficacy. The experimental results demonstrate that when the two pectoral fins on either side flap synchronously or inversely, the robot can generate varying thrust, lift, and lateral forces, enabling smooth advancement and turning. These findings validate the feasibility and efficacy of bionic design, offering innovative concepts and methodologies for underwater robot development.

Gene engineering and combinatorial approaches with other cancer immunotherapy agents may confer capabilities enabling full tumor rejection by adoptive T cell therapy (ACT). The provision of proper costimulatory receptor activity and cytokine stimuli, along with the repression of inhibitory mechanisms, will conceivably make the most of these treatment strategies. In this sense, T cells can be genetically manipulated to become refractory to suppressive mechanisms and exhaustion, last longer and differentiate into memory T cells while endowed with the ability to traffic to malignant tissues. Their antitumor effects can be dramatically augmented with permanent or transient gene transfer maneuvers to express or delete/repress genes. A combination of such interventions seeks the creation of the ultimate bionic T cell, perfected to seek and destroy cancer cells upon systemic or local intratumor delivery.

The intracellular delivery of messenger (m)RNA holds great potential for the discovery and development of vaccines and therapeutics. Yet, in many applications, a major obstacle to clinical translation of mRNA therapy is the lack of efficient strategy to precisely deliver RNA sequence to liver tissues and cells. In this study, we synthesized virus-like mesoporous silica (V-SiO 2 ) nanoparticles for effectively deliver the therapeutic RNA. Then, the cationic polymer polyethylenimine (PEI) was included for the further silica surface modification (V-SiO 2 -P). Negatively charged mRNA motifs were successfully linked on the surface of V-SiO 2 through electrostatic interactions with PEI (m@V-SiO 2 -P). Finally, the supported lipid bilayer (LB) was completely wrapped on the bionic inspired surface of the nanoparticles (m@V-SiO 2 -P/LB). Importantly, we found that, compared with traditional liposomes with mRNA loading (m@LNPs), the V-SiO 2 -P/LB bionic-like morphology effectively enhanced mRNA delivery effect to hepatocytes both in vitro and in vivo , and PEI modification concurrently promoted mRNA binding and intracellular lysosomal escape. Furthermore, m@V-SiO 2 -P increased the blood circulation time (t 1/2 = 7 h) to be much longer than that of the m@LNPs (4.2 h). Understanding intracellular delivery mediated by the V-SiO 2 -P/LB nanosystem will inspire the next-generation of highly efficient and effective mRNA therapies. In addition, the nanosystem can also be applied to the oral cavity, forehead, face and other orthotopic injections.

Inspired by the vertebrate immune system, artificial immune system (AIS) has emerged as a new branch of computational intelligence. This paper explores the application of AIS in the problem of assembly planning and proposes a novel approach, called the immune optimization approach (IOA), to generate the optimal assembly plan. Based on the bionic principles of AIS, IOA introduces manifold immune operations including immune selection, clonal selection, inoculation and immune metabolism to derive the optimal assembly sequence. Maintenance of population diversity, attention to the local as well as the global search, and employment of heuristic knowledge to direct the search of optimized assembly sequences are the major concerns of IOA. The details of IOA are presented and the immune operations are discussed. Two practical products are taken as examples to illustrate the validity of IOA in assembly planning, and encouraging solutions in quality and efficiency are achieved. Comparisons with genetic algorithm demonstrate that IOA finds the optimal assembly solution or near-optimal ones more reliably and more efficiently, indicating that IOA has potential and advantages in dealing with assembly planning.

The Centre has many uses for the steam that comes from its two Miura boilers, explains Kevin Edwardson, project engineering manager at Bioniche Life Sciences Inc. Our vaccine production facility uses air conditioned clean rooms where we grow and harvest cells. Since the Centre is new, we have no way of knowing how much our Miura boilers will save us in fuel, but I can report that we are happy with these boilers and look forward to seeing savings in the future.