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Resumo O artigo discute o uso da inscrição em restos a pagar e seus impactos na credibilidade e na transparência do orçamento público. A partir de uma tipificação dos quatro tipos de carry-over existentes na regulação brasileira, articularam-se evidências do surgimento de um “orçamento paralelo” nos três níveis de governo com aspectos de regulamentação e controle externo. A evolução dos saldos e da inscrição de restos a pagar foi captada pelos saldos dessas contas no governo federal, nos governos de 26 estados e Distrito Federal, e em cerca de 4.100 municípios. Adicionalmente, observaram-se a interpretação dada pelos Tribunais de Contas para a inscrição em restos a pagar sem lastro financeiro, assim como o nível de transparência dada pelos governos com maiores saldos de restos a pagar. Os resultados indicam que, além do crescente endividamento, a fraca regulação sobretudo dos restos a pagar não processados está reduzindo seriamente a credibilidade e a transparência do orçamento em todos os níveis de governo.

Liberalized concealed carry laws test how firearm legislation affects crime in the US. This study analyzes the relationship between these laws, total homicide, and firearm homicide using panel data from 1980 to 2018 across all 50 states and the District of Columbia. The analysis uses multivariate regression with state and time-fixed effects and applies a general-to-specific procedure to select control variables. Robustness checks, including a generalized synthetic control model, confirm the findings. The results show no statistically significant relationship between shall-issue or permitless carry laws and homicide rates, even at the 10% level. The findings remain consistent across alternative model specifications. If these effects are truly null, liberalized concealed carry laws may have positive social implications, offering valuable insights for policymakers. 

This paper details the approach to the efficient design and optimization of a reversible adder and multiplier utilizing Peres gates, which is a three-input, three-output gate. Peres gates are recognized for their universality and energy-efficient properties and present an intriguing option for constructing reversible circuits. Reversible logic is characterized by its ability to uniquely determine input states from output states. The design methodology involves a cascading arrangement of Peres gates, each performing a reversible XOR operation on corresponding bits of the input numbers and the carry-out from the preceding stage. This paper presents a detailed schematic representation, simulation results, and analysis of the proposed designs of different adders and multipliers, showcasing their potential for reversible applications. The integration of Peres gates in the adder and multiplier design signifies a step forward in the exploration of reversible logic circuits and their applications in contemporary computing paradigms. The overall hardware reduction is the main achievement of this research in terms of quantum cost.

This research aims to fill up the research gap in energy‐efficient transistor‐level wide word‐length carry circuit generator by using ripple carry (RC) and carry look‐ahead (CLA) method‐based hybrid 4‐bit carry generation process for wide word‐length carry‐select adder (CSLA). Compared to the existing 4‐bit CLA architectures, the proposed 4‐bit RC‐CLA method‐based hybrid 4‐bit carry generator showed performance improvement in terms of power and power delay product (PDP). Later, the 4‐bit carry architectures (existing and proposed) were used as a base to implement 16‐bit carry select adder (CSA) in order to investigate and compare the effect of using the proposed hybrid RC‐CLA based 4‐bit carry generator in large structures. Unlike 4‐bit operation, the proposed design displayed the best performance in power and PDP for 16‐bit CSA extension, which proves its effectiveness in wide word‐length adder structures. This research aims to fill up the research gap in energy‐efficient transistor‐level wide word‐length carry circuit generator by using ripple carry (RC) and carry look‐ahead (CLA) method‐based hybrid 4‐bit carry generation process for wide word‐length carry‐select adder (CSLA). Compared to the existing 4‐bit CLA architectures, the proposed 4‐bit RC‐CLA method‐based hybrid 4‐bit carry generator showed performance improvement in terms of power and power delay product (PDP).

1. Many studies have reported honeybee colony losses in human-dominated landscapes.While bee floral food resources have been drastically reduced over past decades in humandominatedlandscapes, no field study has yet been undertaken to determine whether there is acarry-over effect between seasonal disruption in floral resource availability and high colonylosses.2. We investigated if a decline in the harvest of pollen by honeybees in spring affected managedhoneybee colony dynamics (brood size, adult population and honey reserves) and health(Varroa mite loads and colony survival) throughout the beekeeping season.3. A decline in pollen harvest was associated with a direct reduction in brood production,leading to a negative effect on the adult population size later in the season, and lower honeyreserves before the onset of winter. Furthermore, the decline in pollen harvest negativelyimpacted the health of the colony, resulting in higher Varroa mite loads and higher seasonaland winter colony losses.4. Early-warning signs of these carry-over effects were identified, showing that preferentialinvestment in honey reserves instead of brood production early in the season increased thedecline in pollen harvest and its associated carry-over effects.5. Synthesis and applications. The results suggest that the decline in pollen harvest may havebeen overlooked as a cause of pollen shortage and associated bee colony losses. Strategies toavoid such losses in intensive farmland systems include (i) limiting or avoiding honey harvestsin spring, (ii) monitoring colonies for early-warning signals of colony failure and (iii) increasingthe amount of floral resources available through wise land-use management.

Mortality and shifts in species distributions are among the most obvious consequences of extreme climatic events. However, the sublethal effects of an extreme event can have persistent impacts throughout an individual’s lifetime and into future generations via within-generation and transgenerational phenotypic plasticity. These changes can either confer resilience or increase susceptibility to subsequent stressful events, with impacts on population, community, and potentially ecosystem processes. Here, we show how a simulated extreme warming event causes persistent changes in the morphology and growth of a foundation species (eelgrass, Zostera marina) across multiple clonal generations and multiple years. The effect of previous parental exposure to warming increased aboveground biomass, shoot length, and aboveground–belowground biomass ratios while also greatly decreasing leaf growth rates. Long-term increases in aboveground–belowground biomass ratios could indicate an adaptive clonal transgenerational response to warmer climates that reduces the burden of increased respiration in belowground biomass. These transgenerational responses were likely decoupled from clonal parent provisioning as rhizome size of clonal offspring was standardized at planting and rhizome starch reserves were not impacted by warming treatments. Future investigations into potential epigenetic mechanisms underpinning such clonal transgenerational plasticity will be necessary to understand the resilience of asexual foundation species to repeated extreme climatic events.

1. Early arrival at breeding grounds is of prime importance for migrating birds as it is known to enhance breeding success. Adults, males and higher quality individuals typically arrive earlier, and across years, early arrival has been linked to warmer spring temperatures. However, the mechanisms and potential costs of early arrival are not well understood. 2. To deepen the understanding of arrival date differences between individuals and years, we studied them in light of the preceding spring migration behaviour and atmospheric conditions en route. 3. GPS and body acceleration (ACC) data were obtained for 35 adult white storks (Ciconia ciconia) over five years (2012-2016). ACC records were translated to energy expenditure estimates (overall dynamic body acceleration; ODBA) and to behavioural modes, and GPS fixes were coupled with environmental parameters. 4. At the interindividual level (within years), early arrival was attributed primarily to departing earlier for migration and from more northern wintering sites (closer to breeding grounds), rather than to migration speed. In fact, early-departing birds flew slower, experienced weaker thermal uplifts and expended more energy during flight, but still arrived earlier, emphasizing the cost and the significance of early departure. Individuals that wintered further south arrived later at the breeding grounds but did not produce fewer fledglings, presumably due to positive carryover effects of advantageous wintering conditions (increased precipitation, vegetation productivity and daylight time). Therefore, early arrival increased breeding success only after controlling for wintering latitude. Males arrived slightly ahead of females. Between years, late arrival was linked to colder temperatures en route through two different mechanisms: stronger headwinds causing slower migration and lower thermal uplifts resulting in longer stopovers. 5. This study showed that distinct migratory properties underlie arrival time variation within and between years. It highlighted (a) an overlooked cost of early arrival induced by unfavourable atmospheric conditions during migration, (b) an important fitness trade-off in storks between arrival date and wintering habitat quality and (c) mechanistic explanations for the negative temperature–arrival date correlation in soaring birds. Such understanding of arrival time can facilitate forecasting migrating species responses to climate changes.

Colonization of gut microbiomes during early life can shape metabolism and immunity of adult animals. However, most data are derived from antibiotic‐treated or germ‐free laboratory mammals. Furthermore, few studies have explored how microbial colonization during critical windows influences a suite of other fitness‐related traits in wild animals. This study tested whether hatching constitutes a critical developmental window for gut microbiome colonization in wild‐caught amphibians and whether perturbations to gut microbiota at hatching shape fitness‐related traits of larval growth, metabolism, metamorphosis and disease susceptibility. We sterilized wood frog eggs and then inoculated them with microbes from differing sources, including from another species (bullfrogs) that differ in disease resistance and life history. We measured development, growth and metabolic rates through metamorphosis among individuals from each microbial treatment. A separate group was exposed to an LD50 dose of ranavirus—an emerging disease—to test for microbiome effects on disease susceptibility. We also quantified rates of deformities to test for microbial treatment effects on overall health. Manipulation of microbiota on eggs altered the trajectory of gut microbiome communities across larval ontogeny, though disruption appeared to be transitory. While microbiome structure converged among the treatments by metamorphosis, the effects of disruption on host phenotypes persisted. Larvae inoculated with the bullfrog gut microbiota exhibited accelerated growth and development rates compared to controls. By contrast, sterilized larvae maintained in sterile water for several days after hatching exhibited greater disruption to their gut microbiota across ontogeny, as well as altered metabolism, more tail deformities, and were more likely to die when exposed to an LD50 dose of ranavirus compared to the other treatments. These results suggest perturbations to the microbiota during critical developmental windows can alter the trajectory of the gut microbiome, and have long‐term effects on fitness‐related traits in larval amphibians. These results suggest that explicit tests of how changes in the composition and abundance of the microbial community shape phenotypes across ontogeny in amphibians could shed light on host–microbe interactions in wildlife, as well as inform conservation efforts to mitigate emerging diseases. This study shows that hatching is apparently a critical developmental window for bacterial colonization of larval amphibian guts. Perturbation at hatching alters the trajectory of gut microbiomes across ontogeny and shapes host phenotypes. Altered gut microbiota influenced larval amphibian growth, development, metabolism, morphological asymmetry and susceptibility to an emerging disease.

Birth timing is a key life-history characteristic that influences fitness and population performance. For migratory animals, however, appropriately timing birth on one seasonal range may be constrained by events occurring during other parts of the migratory cycle. We investigated how the use of capital and income resources may facilitate flexibility in reproductive phenology of migratory mule deer in western Wyoming, USA, over a 5-yr period (2015–2019). Specifically, we examined how seasonal interactions affected three interrelated life-history characteristics: fetal development, birth mass, and birth timing. Females in good nutritional condition at the onset of winter and those that migrated short distances had more developed fetuses (measured as fetal eye diameter in March). Variation in parturition date was explained largely by fetal development; however, there were up to 16 d of plasticity in expected birth date. Plasticity in expected birth date was shaped by income resources in the form of exposure to spring green-up. Although individuals that experienced greater exposure to spring green-up were able to advance expected birth date, being born early or late with respect to fetal development had no effect on birth mass of offspring. Furthermore, we investigated the trade-offs migrating mule deer face by evaluating support for existing theory that predicts that births should be matched to local peaks in resource availability at the birth site. In contrast to this prediction, only long-distance migrants that paced migration with the flush of spring green-up, giving birth shortly after ending migration, were able to match birth with spring green-up. Shorter-distance migrants completed migration sooner and gave birth earlier, seemingly trading off more time for offspring to grow and develop over greater access to resources. Thus, movement tactic had profound downstream effects on birth timing. These findings highlight a need to reconsider classical theory on optimal birth timing, which has focused solely on conditions at the birth site.

The tackle contest is the most common and most injurious match contact event in rugby and is an indicator of performance. Tackle Ready is World Rugby's tackle technique education program. Limited research has characterized the tackle contest in women's rugby. The purpose of this study is to: (1) identify the match situational characteristics, ball‐carrier and tackler technical actions demonstrated in elite women's Rugby Union and (2) to determine the extent to which Tackle Ready recommended tackle techniques were exhibited. Technical characteristics for 1500 tackle events in the 2022–2023 Women's Six Nations Championship were visually assessed according to a predefined coding framework and the Tackle Ready program. Tackles lacked full completion (0.2%) of the 22 coded Tackle Ready techniques with 47% of the recommended techniques demonstrated in each tackle on average (range 15%–98%). A high proportion of tackles involved two defenders (48%), approaching ball‐carriers from the side (38%) or oblique angles (39%), in an upright position (30%), and with initial contact made with the arm (51%). Incorrect pre‐contact head positioning and head placement upon contact accounted for 50% and 15% of tackles, respectively, and there was a mean of 14 (95% CI 11–18) head and neck contacts to a tackler and 18 (95% CI 14–22) head and neck contacts to a ball‐carrier per game. Targeted interventions to encourage adoption of recommended techniques are needed to reduce tackle‐related injury risk in women's rugby. This study provides valuable context for future discussion across law enforcement, coach education and gender‐specific tackle coaching in the women's game. Highlights Retrospective video analysis of a random subset of 1500 tackles from all 15 games in the 2022–2023 Women's Six Nations revealed low overall completion of recommended Tackle Ready techniques with 47% of the recommended techniques exhibited in each tackle on average. Incorrect pre‐contact head positioning and head placement upon contact accounted for 50% and 15% of tackles, respectively, and there was a mean of 14 head and neck contacts to a tackler and 18 head and neck contacts to a ball‐carrier per game. A high proportion of tackles involved two defenders (48%) and upright body positions (30%).