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4 result(s) for "ceratoconjuntivite"
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Molecular characterization of bacterial microbiota associated with infectious bovine keratoconjunctivitis in Michoacán, Mexico
Background: The most common ocular disease affecting cattle worldwide is infectious bovine keratoconjunctivitis (IBK), which has been associated with Moraxella bovis bacterium. Objective: To report the molecular characterization of the ocular bacterial microbiota and its relation to IBK in cattle in two dairy regions in Michoacán, Mexico. Methods: A total population of 761 bovines were evaluated, of which 17 (2.23%) showed symptoms of IBK. Thirtyeight bacterial isolates from ocular samples of bovines with IBK were characterized by Gramstaining and antimicrobial sensitivity. In addition, isolates were identified by sequence comparisons of the 16S ribosomal gene. Results: The genus Moraxella was one of the most abundant bacteria and M. bovoculi was the most predominant species. Conclusion: The bacterial isolates identified in eye lesions of cattle and associated to IBK are diverse. To the author´s knowledge, this is the first study on the subject in Mexico; therefore, more research is needed to estimate the incidence of IBK and determine its associated microbiota.
Characterization and antimicrobial resistance of Moraxella ovis isolates from clinical cases of contagious ovine keratoconjunctivitis in Mexico State, Mexico
Antecedentes: La queratoconjuntivitis contagiosa ovina (OKC) causa ceguera temporal o permanente en ovinos y caprinos y está asociada a un conjunto de géneros bacterianos, algunos de los cuales muestran resistencia antimicrobiana. Objetivo: Identificar la relación fenotípica-genotípica de la resistencia antimicrobiana de aislamientos Moraxella ovis obtenidos de casos clínicos de queratoconjuntivitis contagiosa ovina (OKC) en México. Métodos: Se obtuvieron un total de 209 muestras de casos clínicos de OKC en ovinos y se identificaron 60 aislamientos de Moraxella ovis por técnicas bacteriológicas y amplificación de genes 16s rRNA y rtxA por PCR. En todos los aislamientos se evaluó resistencia antimicrobiana mediante prueba de susceptibilidad de difusión en disco y amplificación de genes de resistencia por PCR. Resultados: Se encontraron 14 aislamientos de Moraxella ovis con resistencia antimicrobiana (AMR) y cinco multirresistentes (MDR). Los genes de resistencia antimicrobiana sul1, sul2, tetB, qnrA, qnrB, BlaTEM fueron amplificados, mientras que el gen floR no fue amplificado. Conclusión: Este es el primer reporte de aislamiento de M. ovis en lesiones oculares en ovinos en el Estado de México, con seis genes de resistencia antimicrobiana identificados. Nuestros resultados sugieren que Moraxella ovis juega un papel importante en el curso de la enfermedad y brinda un panorama para su vigilancia epidemiológica molecular y resistencia bacteriana. Background: Contagious ovine keratoconjunctivitis (OKC) causes blindness in sheep and goats and it is associated with a set of bacterial genera of which some species show antimicrobial resistance. Objective: To identify phenotypic-genotypic relationship of antimicrobial resistance from Moraxella spp. isolates obtained from clinical cases of contagious ovine keratoconjunctivitis (OKC) in Mexico. Methods: A total of 209 samples were obtained from clinical cases of OKC in sheep and 60 Moraxella ovis isolates were identified by bacteriological techniques and amplification of 16s rRNA and rtxA genes by PCR. All isolates were evaluated in terms of antimicrobial resistance by the disk diffusion susceptibility test and amplification of resistance genes by PCR. Results: We found 14 Moraxella ovis isolates with antimicrobial resistance (AMR) and five multiresistant (MDR). The sul1, sul2, tetB, qnrA, qnrB, BlaTEM genes of antimicrobial resistance were amplified, while gene floR was not amplified. Conclusion: This is the first isolation report of Moraxella ovis from ocular lesions in sheep in the State of Mexico, with six antimicrobial resistance genes identified. Results suggest that Moraxella ovis plays an important role in the course of the disease and provides a panorama for molecular epidemiological surveillance and bacterial resistance. Antecedentes: A ceratoconjuntivite contagiosa ovina (OKC) é uma doença infeccioso contagioso que causa cegueira temporária ou permanente em ovinos e caprinos, esta doença está associada a um conjunto de gêneros bacterianos dos quais alguns deles relataram resistência antimicrobiana. Objetivo: O objetivo deste estudo foi identificar a relação fenotípica-genotípica da resistência antimicrobiana de Moraxella spp. isolados obtidos de casos clínicos de ceratoconjuntivite contagiosa ovina (OKC) no estado do México. Métodos: Um total de 209 amostras foram obtidas de casos clínicos de OKC em ovinos.e obtidos e 60 isolados de Moraxella ovis foram identificados por técnicas bacteriológicas e amplificação dos genes 16s rRNA e rtxA por PCR. Todos os isolados foram avaliados quanto à resistência antimicrobiana pelo método de teste de suscetibilidade à difusão em disco e pela amplificação de genes de resistência por PCR respectivamente. Resultados: Determinamos 14 isolados de Moraxella ovis com resistência antimicrobiana (AMR) e cinco multirresistentes (MDR) e amplificou os genes de resistência antimicrobiana sul1, sul2, tetB, qnrA, qnrB, BlaTEM e não amplificou o gene floR. Conclusão: É o primeiro relato de isolamento de Moraxella. ovis em lesões oculares em ovinos no Estado do México e a identificação de seis genes de resistência antimicrobiana. Sugere-se que Moraxella ovis. desempenha um papel importante no curso da doença e fornece um panorama de interesse em vigilância epidemiológica molecular e resistência bacteriana.
Ocular lesions in HTLV-1 infected patients from Salvador, State of Bahia: the city with the highest prevalence of this infection in Brazil Lesões oculares em pacientes infectados pelo HTLV-1 em Salvador, Estado da Bahia: a cidade com maior prevalência desta infecção no Brasil
In order to determine the prevalence of ocular lesions in HTLV-1 infected patients in Salvador Bahia, a transversal study was conducted on 140 HTLV-1 infected patients (90 asymptomatic and 50 tropical spastic paraparesis/HTLV-1-associated myelopathy) between June 2004 and November 2005. The ophthalmological examination included visual acuity measurement, ocular motility, biomicroscopy of the anterior and posterior chambers, intraocular pressure and evaluation of lachrymal secretion. Observation verified 4 (2.8%) out of 140 patients with uveitis (two patients had intermediate uveitis and two had pan-uveitis) and 39 (36.4%) out of 107 patients with keratoconjunctivitis sicca. The prevalence of Keratoconjunctivitis sicca was significantly higher among the TSP/HAM patients (OR age adjusted=3.64; 95%CI 1.59-8.32). Uveitis and corneal opacities were also important findings, indicating the strong need for periodic ophthalmological follow-up in all HTLV-1 subjects.Com o objetivo de determinar a prevalência de lesões oculares, em portadores de HTLV-1 em Salvador, Bahia, foi realizado um estudo transversal em 140 pacientes (90 assintomático e 50 com paraparesia espática tropical/mielopatia associada ao vírus linfotrópico de células T humanas) entre junho de 2004 e novembro de 2005. O exame oftalmológico incluiu medida da acuidade visual, exame da motilidade ocular, biomicroscopia anterior e posterior, pressão intraocular e avaliação do filme lacrimal. Observamos 4.0 (2.8%) pacientes com uveites (dois com uveíte intermediária e dois com panuveíte) e 39 (36,4%) pacientes com ceratoconjuntivite seca. A prevalência de Ceratoconjuntivite seca foi significantemente mais elevada entre os pacientes com TSP/HAM (RC ajustada para idade = 3,64; IC 95% 1,59-8,32). As uveítes e opacidades corneanas foram também, patologias importantes, indicando a necessidade de acompanhamento oftalmológico periódico nos portadores de HTLV-1.
Antibiotic susceptibility of Moraxella bovis recovered from outbreaks of Infectious Bovine Keratoconjunctivitis in Argentina, Brazil and Uruguay between 1974 and 2001
Antibiotic susceptibility of thirty Moraxella bovis strains recovered from outbreaks of Infectious Bovine Keratoconjunctivitis (IBK) in Argentina, Brazil and Uruguay between 1974 and 2001 was determined using the Kirby-Bauer and the Minimal Inhibitory Concentration (MIC) methods. Our results suggest that most strains were susceptible to the antibiotics used in the treatment of IBK, and that the antibiotic susceptibility of M. bovis varied with the geographical region and period of recovery. A susceptibilidade a antimicrobianos de trinta cepas de Moraxella bovis recuperadas entre 1974 e 2001 em surtos de Ceratoconjuntivite Infecciosa Bovina (CIB) ocorridos na Argentina, Brasil e Uruguai foi determinada pelos métodos de Kirby-Bauer e Concentração Inibitória Mínima. Nossos resultados indicam que a maioria das cepas é susceptível aos antibióticos utilizados no tratamento da CIB e que a susceptibilidade antimicrobiana da M. bovis variou conforme a região geográfica e período de recuperação.